Objective To investigate the clinical significance of the rate of spontaneous apoptosis and the expression of p27kip1 in transitional cell carcinoma of bladder (BTCC). Methods Immunohistochemical analysis of p27kip1 was performed on paraffin embedded tissue sections in 50 cases of BTCC, by immunohistochemical method S-P. The terminal deoxynucleotidyl transferase- mediated dUTP- biotin nick end labeling (TUNEL) was used to examine the level of apoptotic cells in 50 cases of BTCC. Results In BTCC, the spontaneous apoptosis index AI was (3.0?1.5)%, the positive rate of p27kip1 was 58.0 %(29/50). Both of them decreased with the escalation of the clinicopathologic grade and stage. The positive expression of p27kip1 protein were significantly associated with higher spontaneous apoptosis (P

AIM: In order to observe the curative effect of Fugan Tablet on chronic hepatitis B and hepatic fibrosis,we held clinical observation of 120 cases of chronic hepatitis B and hepatic fibrosis. METHODS: 120 cases of chronic hepatitis B and hepatic fibrosis were divided into curative group with Fugan Tablet and control group with Hepatic Ling Injection,and the change of symptom,hepatic function,iconography index,hepatic fibrosis in serum and the hepatic histology were observed before and after the treatment,then the curative result of the two groups was compared. RESULTS: The total effective percentage of curative group was 88.33%,and the total effective percentage of control group was 68.33%.Comparing the two groups,the difference was obvious(P

Unfolded protein response(UPR) is a protective response in cell endoplasmic reticulum(ER) under stress condition.Three ER transmembrane proteins,IRE1,PERK,and ATF6,coordinately regulate the UPR function in mammalian cells through their signaling pathways.In addition,some proteins and transcription factors during the UPR can provide negative and positive feedback loops to maintain the normal function of ER.UPR can trigger cell death or apoptosis and eventually cause related diseases if the ER stress persists.Several key mediators of UPR are candidates for therapeutic targets in many studies.Up to now progress has been made in the area,which provides new ideas for clinical practice and holds a great potential for future application.

By using thrombolysis therapy,34 patients suffering from actue myocardial infarction were treated .And by comparing chromium,cobalt,nickel,vanadium and molybdenum contents in serum of 34 patients suffering from acute myocardial infarction with that of contrast group and the contents between before-treatment and after-treatment,following results were revealed;treatment before chromium,cobalt,nickel,vanadium and molybdenum contents in serum of the patients were low and there is great difference between the patients and control groups(P0 05).

Longnon-codingRNAs(lncRNAs)areagroupofRNAslongerthan200bpwithoutprotein-coding capacity and play an important role in epigenetics , transcription regulation and post-transcription regulation .Recently, studies have shown that dysregulation of lncRNAs caused cell cycle disorders , and abnormal activities of cell apoptosis and autophagy , contributing to a variety of diseases .Therefore , the role of lncRNAs in regulating cell death is expected to lead to the discovery of potential novel diagnostic markers and therapeutic targets .

Objective: To explore the high dose atorvastatin reducing contrast induced nephropathy (CIN) rate in patiens with emergent percutaneous coronary intervention (PCI) via improveing heat shock protein-90 (HSP90) expression with its possible mechanism. Methods: A total of 158 STEMI patients with emergent PCI in our hospital were studied. The patients were randomly divided into 2 groups: High dose atorvastatin group, the patients received pre-operative atorvastatin 40 mg,n=80 and Control group, the patients received pre-operative placebo,n=78. The serum creatinin (Scr), creatinine clearance rate (Ccr), blood urea nitrogen (BUN), superoxide dismutase (SOD), malondialdehyde (MDA), nitrogen monoxide (NO), HSP90 mRNA expression and protein concentration and urine α1-microglobulin were examined in all patients and the incidence rates of CIN were compared between 2 groups. Results: Compared with Control group, High dose atorvastatin group had drcreased Scr (68.92 ± 8.80) μmol/L vs (77.25 ± 13.36) μmol/L, MDA (3.88 ± 0.53) nmol/L vs (4.08 ± 0.52) nmol/L and urine α1-micrglobulin (1.38 ± 0.36) mg/dl vs (1.89 ± 1.13 ) mg/dl; increased Ccr (89.71 ± 9.85) ml/min vs (77.28 ± 13.78) ml/ min, SOD (129.52 ± 30.63) U/ml vs (117.66 ± 27.98) U/ml, NO (66.23 ± 29.26) μmol?gprot vs (55.12±27.43) μmol?gprot, allP<0.05. Compared with Control group, High dose atorvastatin group presented higher post-operative HSP90 mRNA expression (0.466 ± 0.158) vs (0.224 ± 0.278 ) and protein concentration (1259.83 ± 121.17) pg/ml vs (1195.0 ± 127.65) pg/ml, allP<0.05. The incidence rate of CIN was lower in High dose atorvastatin group (2.5%) than Control group (10.3%),P<0.05. Conclusion: A high dose atorvastatin administration before emergent PCI may decrease CIN occurrence rate. Atorvastatin may promote HSP90 expression, increase NO produciton, then improve the vascular endothelial function and anti-oxidative ability to protect the renal function in STEMI patients.

Many genetic events have been described to be involved in the development of tumor,but a lot of recent researches proved that epigenic events also played an important role in it.DNA methylation is one of the common modification of epigenic events,the binding of methyl-CpG binding domain proteins has been shown to lead to transcriptional repression of many tumor repressor genes.

To investigate the relationship between esophageal motility and GI hormones in patients suffering from gastroesophageal reflux disease (GERD), the esophageal motility in 20 GERD patients with esophagitis and 25 GERD patients without esophagitis was examined by means of SGY-3 Digestive Tract Motility Measuring Instrument. Meanwhile,Serum gastrin,motilin,glucagon,estradiol were measured with RIA.The data from 15 healthy volunteers served as control.The results showed that ①GERD patients without esophagitis had lower LESP(2 12?1 00kPa) than control(3 23?0 72kPa P

To construct a cDNA library of gerbil′s cochlea, the mRNA was separated from the cochlea of gerbil and the first strand cDNA was synthesized through reverse transcription by a modified oligo(dt) primer. The double strand cDNA was amplified by Ld pcR. After cDNA size fractionation , the ds cDNA was ligated in the ? TripIEx2 vector . The cDNA library was identified with special primers of prestin genes of cochlea by PCR .The results showed that the titer of library was 1.8?10 6 pfu and the percentage of recombinant clones was 80% . Prestin gene was contained in the library , the size of the fragment was 863bp . The results suggest that the established cDNA library has a high titer, recombinant percentage and large insert fragments of genes . The study lays the foundation of molecular biological study of the cochlea.

Objective To assess the effect of rhein on diabetic nephropathy in rats with type 2 diabetes mellitus. Methods Diabetes was induced by high-lipids and high-sucrose diets (20% sucrose, 10% lard, 2.5% cholesterol, 1% cholic acid and 66.5 % regular chow, w/w) for 1 month and then intraperitoneal injection with 25 mg/kg streptozoticin(STZ) in female inbred Wistar rats. Rhein was continuously given 100 mg?kg-1?day-1 for 6 months 1 week after STZ injection in rhein-prevented group, and 1 month after STZ injection in rhein-treated group. Blood and urinary biochemistry and renal morphology were evaluated at divers times. Results Rhein ameliorated hypertriglyceridemia and hypercholesterolemia, but had few effect on the level of plasma glucose in both rhein-prevented group and rhein-treated group. Excretion of urinary protein reduced markedly and stable-state plasma glucose (SSPG) level decreased in both groups as compared with diabetic group. Examination of kidney sections from both rhein-prevented group and rhein-treated group showed that the structural lesions of glomeruli including mesangial expansion, diffuse glomerulosclersis and thickening of glomerular basement membrane, as well as artery injury such as arteriolar hyalinosis, atherosclerosis were markedly improved as compared with diabetic group. Morphometry of glomeruli from both rhein-prevented group and rhein-treated group showed that glomerular grow and mesangial hypertrophy occurred in diabetic group were ameliorated. The accumulation of fibronectin examined by immunostaining in glomeruli was reduced in both groups. Conclusion Therapeutic intervention with rhein can halt the progression of diabetic nephropathy and prevent the development of glomerulosclerosis and vascular injuries in this animal model of type 2 diabetes mellitus.