Objective To study the absorption and transport mechanism of ursolic acid(UA) by using Caco-2 monolayer model.Methods Evaluating the transport characteristic through studying whether P-glycoprotein(P-gp) transporter inhibitor Verapamil(VER) exists or not by using Caco-2 cell monolayer as an intestinal epithelial cell model and investigating the effects of uptake time,drug concentration,system temperature,and pH value of culture media on the uptake of UA.The drug concentration was measured by HPLC coupled with UV detector.Transport parameters and apparent permeability coefficients (P_(app)) were then calculated.Results In the concentration range of 10—40?mol/L,the uptake of UA by Caco-2 cell all linearly increased.The P_(app) of UA transported on Caco-2 cell monolayer model significantly changed when the specificity inhibitory of P-gp was added to model and the apparent permeability ratio decreased from 3.445 to 1.386.Conclusion The intestinal absorption of UA is passive diffusion as the dominating process and active transportation mediated by P-gp transporter in Caco-2 cell monolayer model.

Purpose;To observe curative effect and side reaction ,histopathology change of dose - dense combinated pharmorubicin with paclitaxel in advanced breast cancer treatment. Methods ;38 patients with advanced breast cancer diagnosed by pathology, which had e-valuation index, pharmorubicin 50mg/m2 ,d1 , intravenous injection,paclitaxel 175mg/m2,d2,intravenous injection,every 2 weeks as 1 course of treatment,after accepted 2 - 4 course of treatment,all patients were evaluated. Results: All patients were followed up,clinical complete remission( CR) 10. 5% , clinical partial remission 71. 1% , total effective rate 81. 6% . effective rate in patents of initial treatment was much higher than that in patients of retreatmen; effective rate in patents of III stage also was much higher than that in patients of IV stage; low leucocyte and Neutropenia appeared III ,IV side reaction,occupying respectively 63.2% and 71. 1% ;7.9% patients febricity owing to low leucocyte were cured by G - CSF and antibiotics,all patients successfully accomplish chemotherapy. Other side reaction,for instance thrombocytopenia.anaemia,nausea and vomiting etc,mostly displayed I , II toxic reaction,but they can be tolerated. After chemotherapy,cancer cells degeneration . cellular necrosis . retrogression and interstitial fibrosis .inflammatory cell infiltration are obviously changed. Conclusion: dose -dense chemotherapy combinated pharmorubicin with paclitaxel in advanced breast cancer treatment, remission rate was much higher and remission rate can be tolerated.

Objective To study the bioequivalence of domestic ropivacaine mesylate in compared with imported ropivacaine hydrochloride in Beagle dogs.Methods Ropivacaine mesylate and ropivacaine hydrochloride at a dose of 10 ?mol/kg was administered intravenously in a randomized cross-over test.The plasma level of ropivacaine was measured by high performance liquid chromatography(HPLC).The pharmacokinetic parameters of ropivacaine were determined by the soft pack of Practical Pharmacokinetic Program Version 97(3P97).Results The pharmacokinetic parameters of ropivacaine mesylate were T_(1/2?)(3.83?1.89) min,T_(1/2?)(41.08?4.47) min,AUC_(0-200)(148.67?87.51) ?g?ml~(-1)?min~(-1),C_(max)(3.96?1.50) ?g/ml,CL(s)(0.030?0.014) L?kg~(-1)?min~(-1),and these parameters of ropivacaine hydrochloride were(3.09?1.19) min,(38.43?4.41)min,(125.80?81.06) ?g?ml~(-1)?min~(-1),(3.72?1.31) ?g/ml,(0.030?0.013) L?kg~(-1)?min~(-1) respectively.Conclusion The bioavailability of ropivacaine mesylate is equal to that of ropivacaine hydrochloride.

Combined operation and irradiation therapy has been performed on 22 patients with primary liver cancer in our hospital.All these patients were mal s.Age ranged from 15-49 years old with an average of 44.5. Histological dingnoais were. hepatocellular carcinoma in 21 cases and cholangiocarcinoma in 1 case. Seventeen (77.3%) patients were associated with liver cirrhosis, nineteen(86.4%)patients had positive AFP by counter- immunoelectrophoretic method.Clinically, 6 patients were stage I, 11 patients were stage II and 5 patients were stage III. The operative procedures consisted of liver resection in 17 patients (local resection 5, right hemihepatectomy 5, left lateral lobectomy 2, left hemihepatectomy 2, total right hepatectomy 2,and left medial lobectomy 1)and palliative operation in other 5 patients (hepatic artery ligation or embolization in 4 patients and hepatic artery infusion in 1 patient) .Radiotherapy comprised local or whole-liver irradiation with 60Co.Up to May 1983, 19 patients were followed-up, all of them survived more than 6 months.The 1, 2, 3, 4 year survival rates after treatment were 78.9%, 66.7%, 64.3% and 44.4% respectively with a median survival duration of 36 months.Data showed that the encouraging results of this method which were better than liver resection and radiotherapy or any other cmbined methods at present.

98%.Thick myofibers were observed by transmission electron microscopy after induction with 5-aza.Four weeks after transplantation,the left ventricle ejection fraction,the movement extent of left ventricle,left ventricle systolic wall thickening and dp/dt of rabbits were all higher than those of the control group and cardiac infarction model group(P

A method of typing of EsD-PCM_1-CLOI on the same gel electrophoresis has been devel- oped by improving and mixing the individual tests of typing EsD, PGM_1and GLOI. The method supplies a reliable, economical and practical means for more wide-spread use of EsD, PGM_1 and GLOI in the forensic medicine field.

Objective:To clarify the expression and significance of coxsackie-adenovirus receptor (CAR) in myocardia with dilated car- diomyopathy (DCM). Methods:Myocardium specimens derived from 20 patients with DCM underwent orthotopic cardiac transplantation and 5 nor- mal myocardium specimens were collected at autopsy from sudden deaths.In the myocardia,coxsackievirus B (CVB)and adeno- virus (ADV) genes were detected by polymerase chain reaction (PCR),and the expression levels of CAR mRNA and protein were examined by reverse transcription (RT)-PCR and immunohistochemistry,respectively. Results:Viral genomes could be amplified from the myocardia of 12 DCM patients (CVB=7,ADV=5).Viral genomes failed to be detected from myocardia of 5 controls.Therefore,the present experiment had four groups:eoxsackievirus,adenovi- rus,the other and the control.There were no CAR mRNA and protein expression in the other group or normol control group. However,the expression levels of CAR mRNA and protein were remarkedly elevated in coxsackievirus and adenovirus groups, There were no differences between coxsackievirus group and adenovirus group. Conclusion:Upregulated CAR expression can increase the susceptibility of CVB or ADV,which makes viral myoearditis pro- gressing to dilated cardiomyopathy.

OBJECTIVE:To improve the monitoring capability and reporting rate of the adverse drug reactions (ADR) in Shanghai Pudong new area. METHODS: By a retrospective study,a total of 1 906 ADR reports collected from 2006 to 2008 in Shanghai Pudong new area were analyzed statistically. RESULTS: The ADR were more often induced by Chinese patent medicines and antibiotics and in which different organs or systems were involved,and 69.52% were induced intravenously. CONCLUSION: It is urgent to intensify clinic publicity and training on ADR,establish effective ADR monitoring system and thoroughly motivate medical staff's enthusiasm to raise the reporting rate of ADR.

Objective To discuss the clinical effect of intravenous thrombolysis combined with psychological intervention in the treatment of acute cerebral infarction. Methods 84 patients with acute cerebral infarction were randomly selected at the June 2015 to September 2016 in our hospital were divided into experimental group 42 cases, 42 cases in the control group, the control group was given aspirin and Plavix treatment, experimental group received alteplase intravenous thrombolytic therapy combined with psychological intervention, each stage of the NISS scores of the two groups were compared, the two groups of patients with SDS self rating scale score, Barthel index and NDS score were compared. Results The experimental group of stage NISS was significantly better than the control group, the differences between groups was statistically significant (P<0.05); the experimental group with SDS self rating scale score, Barthel index and NDS score was significantly better than the control group, the differences between groups was statistically significant (P< 0.05). Conclusion In the treatment of acute cerebral infarction, the effect of intravenous thrombolysis combined with psychological intervention is obvious, and it is worthy of clinical promotion and application.