A method of typing of EsD-PCM_1-CLOI on the same gel electrophoresis has been devel- oped by improving and mixing the individual tests of typing EsD, PGM_1and GLOI. The method supplies a reliable, economical and practical means for more wide-spread use of EsD, PGM_1 and GLOI in the forensic medicine field.

Objective To analyze the clinical characteristics of coronary artery bypass graft sur-gery for elderly patients over the age of 70 years. Methods The clinical data of 232 elderly patients ( age﹥70 years)who underwent coronary artery bypass graft surgery were collected for analysis,including 208 cases of simple coronary artery bypass graft surgery,11 cases of additional mitral valve replacement or annuloplasty,6 cases of additional aortic valve replacement,1 case of additional mitral and aortic valve re-placement,5 cases of additional mediastinal or pericardial tumor resection and 1 case of additional aneu-rysm resection. Results There were eight cases of postoperative respiratory failure,six cases of poor wound healing,4 cases of secondary exploration for postoperative bleeding,2 cases of perioperative myocar-dial infarction,1case of third-degree atrioventricular block and 1 cases of cardiac tamponade. Deaths oc-curred in 2 cases. Conclusion For elderly patients over the age of 70 years,overall effect of coronary ar-tery bypass graft surgery is satisfied. It can improve the quality of life and prolong survival time.

Objective To study the bioequivalence of domestic ropivacaine mesylate in compared with imported ropivacaine hydrochloride in Beagle dogs.Methods Ropivacaine mesylate and ropivacaine hydrochloride at a dose of 10 ?mol/kg was administered intravenously in a randomized cross-over test.The plasma level of ropivacaine was measured by high performance liquid chromatography(HPLC).The pharmacokinetic parameters of ropivacaine were determined by the soft pack of Practical Pharmacokinetic Program Version 97(3P97).Results The pharmacokinetic parameters of ropivacaine mesylate were T_(1/2?)(3.83?1.89) min,T_(1/2?)(41.08?4.47) min,AUC_(0-200)(148.67?87.51) ?g?ml~(-1)?min~(-1),C_(max)(3.96?1.50) ?g/ml,CL(s)(0.030?0.014) L?kg~(-1)?min~(-1),and these parameters of ropivacaine hydrochloride were(3.09?1.19) min,(38.43?4.41)min,(125.80?81.06) ?g?ml~(-1)?min~(-1),(3.72?1.31) ?g/ml,(0.030?0.013) L?kg~(-1)?min~(-1) respectively.Conclusion The bioavailability of ropivacaine mesylate is equal to that of ropivacaine hydrochloride.

This paper discusses the structure,functions,characteristics and the convenience of individual drugs parcel for seawise soldier.The parcel can be used for peacetime training and wartime service.

Objective To study the absorption and transport mechanism of ursolic acid(UA) by using Caco-2 monolayer model.Methods Evaluating the transport characteristic through studying whether P-glycoprotein(P-gp) transporter inhibitor Verapamil(VER) exists or not by using Caco-2 cell monolayer as an intestinal epithelial cell model and investigating the effects of uptake time,drug concentration,system temperature,and pH value of culture media on the uptake of UA.The drug concentration was measured by HPLC coupled with UV detector.Transport parameters and apparent permeability coefficients (P_(app)) were then calculated.Results In the concentration range of 10—40?mol/L,the uptake of UA by Caco-2 cell all linearly increased.The P_(app) of UA transported on Caco-2 cell monolayer model significantly changed when the specificity inhibitory of P-gp was added to model and the apparent permeability ratio decreased from 3.445 to 1.386.Conclusion The intestinal absorption of UA is passive diffusion as the dominating process and active transportation mediated by P-gp transporter in Caco-2 cell monolayer model.

Objective To improve patients' satisfaction degree with nursing work,understand the psychological characteristics and demands of primipara during parturition period.Methods From January 2008 to January 2010,480 cases of primipara from department of obstetrics of our hospital were selected and divided into the observation group and the control group with 240 cases in each group.The control group adopted routine nursing,while the observation group was given psychological nursing and the Chinese medical nursing.The incidence of urinary retention and satisfaction degree of both groups were compared.Results The psychological characteristics and demands of primipara during primipara period showed no remarkable difference.The incidence of urinary retention was lower and satisfaction degree with nursing work was higher in the observation group than the control group.Conclusions The primiparas have anxiety because of fearing pain during parturition period,psychological nursing combined with Chinese medical nursing can reduce the occurrence of postpartum urinary retention and improve the satisfaction degree with nursing work.

“The second class” in medical college is an important carrier of humanities education.In order to give full play to its function,we must effectively rationalize the organization and management of medical humanities education institutions,not only to broaden the content and form of“ the second class”,but also to establish appropriate evaluation incentives.

There are huge amount of TCM ancient books in libraries of TCM college, which are not only thousands of years of academic essence, but also are guidance for modern TCM theory and clinical practice. At the same time, they reflect Chinese traditional ideology and culture, and are significant to studying of traditional culture connotation. How to integrate ancient books resource and development resource service, which is key point of ancient books working in library. In this paper, some studying ideas are discussed from four aspects:Booklist recording objectively and information perfecting;Compilation of classified catalogue and construction of resource library;Introducing “Comprehensive ancient books” concept to promote integration and configuration of library resource;Investigation and comparison of relative edition for providing information service.

Objective: Discuss different sterilization solution on the disinfection effect of fiberoptic bronchoscopy biopsy forceps. Methods:To ethylene oxide sterilization, glutaraldehyde immersion disinfection and high pressure steam sterilization of three methods of fiberoptic bronchoscopy biopsy forceps disinfection sterilization rate, the contamination rate and use fixed number of year for effect comparison. Results: Qualification rate of epoxy ethane sterilizing method were higher than in glutaraldehyde soaking method and high pressure steam sterilization disinfection method, and the pollution rate is significantly lower than the other two groups, the service life of the equipment increase. Conclusion:Low-temperature ethylene oxide sterilization of fiberoptic bronchoscopy biopsy forceps sterilization effect is good, and the pollution rate is low, service life is longer.

98%.Thick myofibers were observed by transmission electron microscopy after induction with 5-aza.Four weeks after transplantation,the left ventricle ejection fraction,the movement extent of left ventricle,left ventricle systolic wall thickening and dp/dt of rabbits were all higher than those of the control group and cardiac infarction model group(P