1.A STUDY ON THE HEMOSTATIC AND HEALING EFFECTS OF CHITOSAN ON INCISED WOUND
Qingyang GU ; Yabing GAO ; Xiaohu CHEN
Medical Journal of Chinese People's Liberation Army 1981;0(06):-
Incised wounds were made in rabbits, and some of the wounds were dressed with chitosan, and others with gauze. After bleeding stopped, hemoglobin in dressings was measured to determine the hemostaic effect. Wounds were redressed, and healing process was studied histologically. The results showed that less Hb was detected in chitosandressing than in gauze. On day 9 after wounding, the content of granulation tissue and collagen formation in the chitosan dressed wounds was more than that in Vaseline gauze dressed group. It suggests that chitosan dressing is effective in hemostasis for incised wounds and may be helpful in wound healing process.
2.Effect of Biglycan and FAK signal pathway on the proliferation of colon cancer cells and its mechanisms
Xiaojing XING ; Xiaohu GU ; Jingdong XIAO
Practical Oncology Journal 2015;29(5):428-431
Objective To investigate the effect of Biglycan and FAK signal pathway on the proliferation of colon cancer cells in vitro and its possible mechanisms.Methods Biglycan expression vector was constructed and transfected into the colon cancer cell line HCT116.FAK inhibitor was used for cell treatment as well.Cells were divided into 5 groups:control group(HCT116),control group transfected with empty plasmid(Vector),con-trol group with empty plasmid transfected and inhibitor treatment(Vector+PF-562271),group transfected with Biglycan expression vector(Biglycan),group with Biglycan expression vector transfected and inhibitor treatment ( Biglycan+PF-562271) .Treatment duration was 24 hours.The expressions of FAK,p-FAK,PCNA and p53 were detected by Western Blot.The proliferation of cells was detected by MTT.Results The overexpression of Biglycan significantly promoted the proliferation of HCT116 and the phosphorylation of FAK(P<0.01).It signif-icantly up-regulated PCNA and down-regulated p53(P<0.01).The FAK inhibitor PF-562271 treatment could obviously inhibit the proliferation of HCT116,and the regulation of Biglycan on the expression of p-FAK, PCNA.p53 proteins was reversed(P<0.01).Conclusion Biglycan regulates the proliferation of colon cancer cells by promoting the activation of FAK signal pathway.
3.Effects and mechanism of Biglycan and FAK signaling pathway on the invasion and metastasis of colon cancer cells
Xiaohu GU ; Jingdong XIAO ; Xiaojing XING
Practical Oncology Journal 2015;29(5):444-449
Objective To observe the influence of Biglycan on the focal adhesion kinase( FAK) activa-tion and to explore whether it regulates the invasion and metastasis of colon cancer through FAK signaling path-way.Methods The overexpressive plasmid of Biglycan was constructed and then transfected into the colon canc-er cell line HCT116.Meanwhile,FAK inhibitor was used to treat cells.Control group(HCT116),empty plasmid group(Vector),empty plasmid and inhibitor treatment group(Vector +PF -562271),Biglycan overexpression group( Biglycan) ,Biglycan overexpression and inhibitor treatment group( Biglycan+PF-562271) were set.Twen-ty four hours after exposure to inhibitor,the expression of FAK and p-FAK in each group was detected by West-ern Blot.The invasion and metastasis of colon cancer cells was detected by transwell assay.Results Overexpres-sion of Biglycan significantly enhanced the phosphorylation level of FAK and promoted the invasion and metastasis of HCT116(P<0.01).The inhibitor of FAK PF-562271 could significantly reduce the expression of p-FAK and reverse the effect of Biglycan on the invasion and metastasis of colon cancer cells.Conclusion Biglycan reg-ulates the invasion and metastasis of colon cancer cells through activation FAK signaling pathway.
4.Treatment of ankylosing spondylitis with a recombinant human tumor necrosis factor receptor-Fc fusion protein: a multicenter, randomized, double blind, controlled trial
Feng HUANG ; Xiaohu DENG ; Yamei ZHANG ; Jieruo GU ; Chunde BAO ; Jianglin ZHANG ; Dawei HU ; Zhiming LIN ; Chunhua YANG ; Dongfeng LIANG ; Junhua GUO ; Zetao LIAO
Chinese Journal of Rheumatology 2008;12(5):314-320
Objective To evaluate the efficacy and safety profile of a recombinant human tumor necrosis factor receptor: Fc fusion protein in ankylosing spondylitis (AS). Methods This was a multicenter,randomized, double-blind, placebo-controlled trial in the first 6 weeks and then followed by an open-labeled trial in the next 6 weeks. One hundred and forty-three patients of active AS were randomly assigned to receive 25 mg twice-weekly subcutaneous injections of rhTNFR:Fc or placebo for 6 weeks. The primary endpoint was proportion of ASAS20 responders at week 6. The secondary endpoints were the proportion of subjects achieving a BASDAI 20%, BASDAI 50% and BASDAI 70% improvement at week 6. Other secondary endpoints, related to reducing signs and symptoms of AS and improving range of motion and physical function, were evaluated.Results Treatment with rhTNFR:Fc resulted in significant improvement. At 6 weeks, 68% of the 71 patients in the rhTNFR: Fc group had a treatment response, as compared with 28% of those in the placebo group(P<0.01). Improvements over base-line values for other measures of disease activity were significantly greater in the rhTNFR:Fc group, rhTNFR:Fc was well tolerated, The most frequently treatment related adverse event was injection site reaction. Conclusion rhTNFR:Fc has demonstrated consistent evidence of efficacy and is well tolerated in the treatment of active AS.
5.Comparison of preliminary effects of mitral valve replacement and mitral valve repair in hypertrophic obstructive cardiomyopathy
Shuai PANG ; Zonghao CHEN ; Pengchao SANG ; Tengfei GU ; Xiaohu HAN ; Jiahui LI ; Jinda YUAN ; Peipei LIU
Clinical Medicine of China 2020;36(5):460-464
Objective:To compare the preliminary clinical effect of mitral valve replacement and mitral valvuloplasty on hypertrophic obstructive cardiomyopathy with mitral regurgitation.Methods:From January 2010 to December 2013, the patients undergoing cardiac surgery at Bakulev Cardiovascular Surgery Research Center in Russia were randomly divided into two groups: Forty-one patients received left ventricular outflow tract hypertrophy myocardial resection (Morrow operation) combined with mitral valve replacement (MVR) as MVR group; Forty-seven patients received Morrow surgery combined with mitral valve repair (MVr) as MVr group.The primary end point: death, secondary end point: thrombosis complications (cerebral infarction, peripheral arterial embolism), recurrence of mitral regurgitation and left ventricular outflow tract pressure difference were compared between the two groups.Results:In the MVr group, 6 cases were converted to MVR and were excluded from the study.The survival rates of MVR group and MVR group were 78.9% and 96.6%, respectively , and the thromboembolic free survival rates of MVR group and MVr group were 83.2% and 100%, respectively. The differences were statistically significant( P=0.034, 0.026, respectively). There was no significant difference in mitral regurgitation and left ventricular outflow tract pressure difference between MVR group and MVR group 24 months after operation( P=1.000, 0.934, respectively). Conclusion:Operation combined with MVR or MVr is an effective method to relieve left ventricular outflow tract obstruction and mitral regurgitation. Morrow operation combined with MVr can improve survival rate and reduce thrombosis complications.
6.Artificial Intelligence Quantitative Parameters in Predicting Invasion of Lung Adenocarcinoma with Diameter≤2 cm of Ground-Glass Density
Yuhai XIE ; Xiaohu LI ; Weishu HOU ; Xiaoyan GU ; Yinfeng QIAN ; Xu GAO ; Dong HU ; Lidong YOU
Chinese Journal of Medical Imaging 2023;31(12):1288-1292
Purpose To investigate the clinical value of artificial intelligence(AI)quantitative parameters in predicting the invasion degree of lung adenocarcinoma with diameter≤2 cm of ground-glass density.Materials and Methods A total of 80 patients with lung adenocarcinoma with diameter≤2 cm ground-glass density confirmed by pathology from March 2019 to April 2022 were retrospectively analyzed.A total of 90 nodules were rerolled,including 8 adenocarcinomas in situ(AIS),34 minimally invasive adenocarcinomas(MIA)and 48 invasive adenocarcinomas(IAC).They were divided into the experimental group(IAC)and the control group(AIS and MIA).The differences of the AI quantitative parameters such as volume,three-dimensional length diameter,maximum area,maximum CT value,minimum CT value and average CT value were compared between two groups,and the predictive values of AI quantitative parameters for the invasion degree of lung adenocarcinoma was evaluated.Results There were statistically significant differences with age,volume,three-dimensional length diameter,maximum area,maximum CT value and average CT value between the two groups(all P<0.05),but no statistically significant differences in gender and minimum CT value(both P>0.05).Binary Logistic regression analysis showed that the three-dimensional length diameter(odd ratio=2.020,P=0.034)and the maximum CT value(odd ratio=1.008,P=0.013)were independent predictors for lung adenocarcinoma with diameter≤2 cm of ground-glass density.The regression model based on the three-dimensional length diameter and the maximum CT value had the best predictive performance,and its AUC was 0.901.When the critical value was 2.432,its sensitivity and specificity were 93.75%and 71.43%,respectively.Conclusion AI quantitative parameters have a high value in predicting the degree of invasion of lung adenocarcinoma with diameter≤2 cm of ground-glass density,and the combined model with three dimensional long diameter and maximum CT value has the highest diagnostic efficiency.
7.Diagnostic value of CT for head and neck pilomatricoma.
Changliang YU ; Rongjuan GU ; Wen SONG ; Xiaohu LI ; Wanqin WANG ; Bin LIU ; Yongqiang YU ; Email: SHENYUXI@MAIL.HF.AH.CN.
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2015;50(7):594-596
OBJECTIVETo evaluate the CT and pathological features of head and neck pilomatricoma.
METHODSWe retrospectively analyzed the CT findings of 13 patients with head and neck pilomatricoma which were pathologically confirmed. Of them 7 patients received plain CT, 2 patients received contrast-enhancement, and 4 patients received plain CT plus contrast-enhancement.
RESULTSAll the patients presented with solitary tumors, 9 of them occurred in the parotid gland region, one in the left occipital, one in the right forehead, one in the right parietal, and another one in the left eyelid. The maximal diameter of the tumors ranged from 0.6 to 2.3 cm with a mean of 1.5 cm. All lesions were well-circumscribed, with partial attachment to the overlying skin. Most of lesions were isodense relative to adjacent skeletal muscle on non-enhanced CT scans, sand-like or nodular calcifications within the masses were showed in 8 cases, and no cystic degeneration was found. On contrast-enhanced imaging, mild to moderate homogenous enhancement was showed in 3 cases, heterogeneous moderate enhancement with patchy nonenhancing necrosis area in one case, marked enhancement in one case, and complete calcification with no enhancement in one case.
CONCLUSIONHead and neck pilomatricoma presents with certain characteristics on CT, which are useful in the clinical diagnosis and differential diagnosis.
Diagnosis, Differential ; Hair Diseases ; diagnosis ; Head and Neck Neoplasms ; diagnosis ; Humans ; Magnetic Resonance Imaging ; Pilomatrixoma ; diagnosis ; Retrospective Studies ; Skin Neoplasms ; diagnosis ; Tomography, X-Ray Computed
8.Automated Pre-delineation of CTV in Patients with Cervical Cancer Using Dense V-Net.
Wen GUO ; Zhongjian JU ; Wei YANG ; Shanshan GU ; Jin ZHOU ; Xiaohu CONG ; Jie LIU ; Xiangkun DAI
Chinese Journal of Medical Instrumentation 2020;44(5):409-414
We use a dense and fully connected convolutional network with good feature learning in small samples, to automatically pre-deline CTV of cervical cancer patients based on CT images and evaluate the effect. The CT data of stage IB and IIA postoperative cervical cancer with similar delineation scope were selected to be used to evaluate the pre-sketching accuracy from three aspects:sketching similarity, sketching offset and sketching volume difference. It has been proved that the 8 most representative parameters are superior to those with single network and reported internationally before. Dense V-Net can accurately predict CTV pre-delineation of cervical cancer patients, which can be used clinically after simple modification by doctors.
Automation
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Female
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Humans
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Machine Learning
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Patients
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Tomography, X-Ray Computed
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Uterine Cervical Neoplasms/diagnostic imaging*
9.Management of cytokine release syndrome related to CAR-T cell therapy.
Hongli CHEN ; Fangxia WANG ; Pengyu ZHANG ; Yilin ZHANG ; Yinxia CHEN ; Xiaohu FAN ; Xingmei CAO ; Jie LIU ; Yun YANG ; Baiyan WANG ; Bo LEI ; Liufang GU ; Ju BAI ; Lili WEI ; Ruili ZHANG ; Qiuchuan ZHUANG ; Wanggang ZHANG ; Wanhong ZHAO ; Aili HE
Frontiers of Medicine 2019;13(5):610-617
Chimeric antigen receptor T (CAR-T) cell therapy is a novel cellular immunotherapy that is widely used to treat hematological malignancies, including acute leukemia, lymphoma, and multiple myeloma. Despite its remarkable clinical effects, this therapy has side effects that cannot be underestimated. Cytokine release syndrome (CRS) is one of the most clinically important and potentially life-threatening toxicities. This syndrome is a systemic immune storm that involves the mass cytokines releasing by activated immune cells. This phenomenon causes multisystem damages and sometimes even death. In this study, we reported the management of a patient with recurrent and refractory multiple myeloma and three patients with acute lymphocytic leukemia who suffered CRS during CAR-T treatment. The early application of tocilizumab, an anti-IL-6 receptor antibody, according to toxicity grading and clinical manifestation is recommended especially for patients who suffer continuous hyperpyrexia, hypotensive shock, acute respiratory failure, and whose CRS toxicities deteriorated rapidly. Moreover, low doses of dexamethasone (5-10 mg/day) were used for refractory CRS not responding to tocilizumab. The effective management of the toxicities associated with CRS will bring additional survival opportunities and improve the quality of life for patients with cancer.