Objective:To investigate the expression and clinical significance of glycosylated albumin(GA) and glycosylated hemoglobin(HbA1c) in patients with diabetic microangiopathy(MVCD).Methods:A total of 295 patients with type 2 diabetes admitted to Chaoyang Central Hospital of Liaoning Province from May 2017 to July 2019 were selected as case group, and they were divided into simple diabetes group (217) and microvascular disease group (78) according to whether the patients had microvascular lesions.Two hundred healthy people were selected as normal control group.The levels of GA and HbA1c were compared between the case group and the normal control group, and the levels of GA and HbA1c in the diabetic group and the microvascular disease group were compared.The risk factors of microangiopathy in patients with type 2 diabetes mellitus were analyzed by logistic regression analysis.Results:The levels of GA and HbA1c were (23.9±10.1)% and (8.6±2.3)% in the case group, which were (13.4±1.2)% and (5.0±0.6)% in the normal control group.The differences between the two groups were statistically significant( t=14.628, 21.636, all P<0.01). The levels of GA and HbA1c were (22.8±9.7)% and (8.4±2.0)% in the simple diabetes group and (25.4±10.3)% and (9.2±2.4)% in the microvascular disease group.The differences between the two groups were statistically significant( t=1.997, 2.869, P=0.047, 0.004). Logistic regression analysis showed that GA and HbAlc were risk factors for microangiopathy in patients with type 2 diabetes ( OR (95% CI) values were 1.088 (1.030-1.157) and 15.251 (1.756-131.157), P=0.003 and 0.014). Conclusion:The occurrence of MVCD is related to GA and HbA1c.Logistic regression analysis showed that GA and HbA1c levels were risk factors of MVCD, and could be used as predictive factors of MVCD.

Objective To study the expression of clock genes in Parkinson's disease(PD) and also the molecular clock machinery of PD.Methods Seventeen PD patients(nine men,eight women)and sixteen agematched controls(nine men,seven women) were investigated in this study.Bload samples were collected over a 12h span at 21:00,00:00,06:00 and 09:00.Using a real-time PCR assay,the peripheral molecular clock was examined by measuring Bmall and Bmal2 expression in total leukocytes during the dark span(from 21:00 to 09:00)in PD patients and age-matched healthy controls.Results At PD,the relative abundance of Bmall was significantly lower at 21:00,00:00 and 06:00(21:00:(22.17±4.09)vs(51.14±8.31),P=0.003,00:00:(30.30±5.45)vs(100.00±24.71),P=0.008,06:00:(19.02±3.33)vs(65.61±14.11),P=0.002).The relative Bmal2 levels in PD patients were significantly less abundant than controls at 21:00 and 00:00(21:00:(48.09±7.40)vs(84.96±9.34),P=0.005;00:00:((65.85±7.88)vs(100.00±11.78),P=0.025).Conclusion These results suggest that a peripheral molecular clock is altered in PD patients.In addition,the relative abundance of Bmall and Bmal2 was significantly lower in PD patients versus control subjects,which can provide a molecular basis to help monitor disease progression and response to investigational drugs.

Objective To study whether suprachiasmatic nucleus (SCN) slices are able to induce the molecular oscillations in NIH/3T3 fibroblast. Methods SCN slices from 10-day-old SD rat and NIH/3T3 cells were co-cultured in a serum-free condition. 24h mRNA profiles of Per1 and Rev-Erbα were measured in NIH/3T3 cells using real-time PCR. Results After co-cultured for 6 days, ten SCN slices can induce the significant daily oscillation of Per1 and Rev-Erba mRNA expression in NIH/3T3 cells (P<0.01). The peak time Rev-erbα and Per1 were at CT5 and CT11 respectively. Rev-Erbα oscillations were significant even with two SCN slices and 2 days co-culture (P<0.05). In contrast, Per1 expression fluctuation was not observed until more than 6 days of co-culture and with six SCN slices (P=0.031). Conclusion Diffusible signals release from SCN slices can regulate molecular rhythms in cultured fibroblasts. Rev-Erbα and Per1 don't start to oscillate at the same time, and Rev-Erbα is more sensitive to SCN signal.

Objective To investigate if basic fibroblast Growth factor (bFGF) can penetrate placental barrier, alleviate rat brain damage and stimulate fetal rat neurons proliferation. Methods BFGF labeled with 125 I was injected peritoneally to pregnant rats, and tissue distribution of radioactivity of 125 I in different organs was detected. Thirty two of pregnant Waister rats were randomly divided at 15 d of gestational age into four groups: normal control, uteri distressed control, bFGF treatment, and bFGF prevention. Fetal rats in the latter three groups suffered from distress in uteri in an animal model of perinatal asphyxia. Proliferated neurons in fetal rat brains were counted in each group. Results 125 I bFGF has been found in fetal brain, heart, lung and spleen, etc. Under high power microscope, proliferated neurons in fetal rat brains were counted in each group, they are: (4.5 ?2.4), (5.8?3.1), (17.2?5.4); (18.1?5.8), ( F=128,P

Objective: To investigate if bFGF can penetrate placental barrier. Methods: Sixteen day pregnant Wistar rats were selected. bFGF labeled with 125 I was injected peritoneally into the rats. The radioactivity of bFGF in different organs were determined in 30 min. Results: (1) 125 I bFGF was detected in the brain, heart, liver,lung and spleen. (2)With the same dose of 125 I bFGF, the concentration of it in the brain was at lowest level of all other organs.(3) In the range of safe dose, the permeability of bFGF through placental barrier was increased obviously. Conclusion: bFGF may penetrate placental barrier into rat's brain, which makes possible for the therapeutic intervention of bFGF in feotus. [

In order to study the changes of main oxidase and antioxidase in the pathological scars,the tissues of hypertrophic scar(10 cases),keloid(10 cases)and normal skin(8 cases)were obtained.The concentration of malonaldehyde(MDA)and the activities of xanthine oxidase(XO),copper,zinc-superoxide dismutase(CuZn-SOD),catalase(CAT)as well as glutathione peroxidase(GPX)were detected by spectrophotometric method.Compared with normal skin tissues,the concentration of malonaldehyde and xanthine oxidase activity were significantly higer in pathological scars(P

Objective To isolate and identify side population (SP) cells like cancer stem cell from human pancreatic cancer cell line SW1990, for the purpose of further evaluation of their biological characteristics. Methods Cell suspension was stained with Hoechst 33342 and PI. Then SP cells were analyzed in the fluorescence activated cell sorter. Cell growth viability was measured by MTT. Stem cell marker CD133 was determined by flow cytometry. Cloning forming efficiency was determined by cloning plating. Expression of ABCG2 protein was detected by Western blot analysis. Results The proportion of SP cells was 2.7%, however it could be completely blocked by verapamil. 9 days later, the value of A492 of SP cells was 2.1, the cloning forming efficiency was (38.7 ± 6.8) % , the positive rate of CD133 was 69.63%, which were significantly higher than cells 0. 5, ( 15.5 ± 2.8)%, 16.71% of corresponding non-SP( P <0.05). The expression of ABCG2 in SP cells was significantly higher than that in non-SP cells. Conclusions SP cells existed in human pancreatic cancer cells SW1990.

Objective To explore the association between late-onset sporadic Parkinson' s disease (PD) and single nucleotide polymorphisms (SNPs) of Ca2+-dependent protease calpain inhibitor calpastatin (CAST) gene in a Chinese Han population.Methods 370 evaluable patients (221 male,149 female) with PD (mean age 65.2 ± 8.5 years) and 390 neurologically healthy controls (208 male,182 female) matched for gender,ethnicity,and area of residence.PD cases were identified from the PD cohort of the Chinese National Consortium on Neurodegenerative Diseases (www.chinapd.cn).A total of 24 tag-SNPs were genotyped capturing 95％ of the genetic variation across the CAST gene.Results There was no association found between any of the polymorphisms and PD in all models tested (co-dominant,dominant-effect and recessive-effect (P ＞ 0.05)).Similarly,none of the common haplotypes was associated with a risk for PD(P ＞ 0.05).Conclusion Results show no significant association between the CAST gene polymorphisms and late onset sporadic PD in the present population.

OBJECTIVE To find out about the transverse section use rate of antimicrobials in our hospital. METHODS A transverse section survey was made from midnight on June 24,2004 to midnight on June 25,2004 within all the inpatients. RESULTS Among 1132 inpatients,the transverse section use rate of antimicrobials was 58.83%,the combined medication ratio being 34.08%.Of all antimicrobial takers 65.92% were given single drug,31.68% double,and 2.40% were given triple.Among all departments of the hospital,the universal ICU had the highest use rate which was 100.00%,next to which were those in the departments of nephrology and gynecology,being respectively 90.00% and 87.23%.The antimicrobial use rate in surgical department(69.68%) was remarkably higher than that in medical department(43.08%).The departments of ophthalmology and otorhinolaryngology had the highest prophylactic use rate which was 83.64%.Rate of etiological examination for therapeutic medication in the whole hospital was 35.97%. CONCLUSIONS The transverse section use rate of antimicrobial in our hospital is slightly higher than the national average use rate.The constituent ratio of prophylactic medication is on the high side and the rate of etiological examination for therapeutic medication is on the low side.Management should be further strengthened for rational use of antimicrobials.

Objective To study the possible relationship between human papillomaviruse (HPV) infection and external genital proliferative diseases. Methods HPV DNA was detected using polymerase chain reaction (PCR) with a consensus primer and typed by using restriction fragment length polymorphism (RFLP) method in 151 paraffin-embedded biopsy specimens from seven kinds of external genital epidermal proliferative lesions. Results In 30 cases of condyloma acuminatum, HPV DNA was positive for all cases, among which HPV6 and HPV11 accounted for 60% and 26.7%, respectively. In 40 cases of bowenoid papulosis, 5 cases of genital Bowen′s disease and 6 cases of erythroplasia of Queyrat, the positive rates of HPV DNA were 55%, 100% and 33.3%, respectively, and the predominant type was HPV16. In 18 cases of external genital invasive squamous cell carcinoma HPV DNA positive rate was 27.8%, and HPV16 was detected in all of the 5 HPV-positive cases. HPV DNA was negative in 32 cases of leukoplakia and 20 cases of extramammary Paget′s disease. Conclusions Condyloma acuminatum is mainly caused by HPV6 infection, followed by HPV11; HPV16 infection is closely associated with the pathogenesis of bowenoid papulosis and external genital squmous cell carcinoma including Bowen′s disease and erythroplasia of Queyrat. In external genital invasive squamous cell carcinoma, HPV16 infection may be one of the multiple carcinogenic factors. No clear relationship is found between HPV infection and leukoplakia or extramammary Paget′s disease.