An established FTA/PCR protocol was applied to detect Cryptosporidium oocysts which were added in beverage, and proved to be as sensitive as 1.0 oocyst/ml detected within 6 hours. The study indicates that this technique is sensitive, time-saving and easy to perform.

Objective By checking on the government employees and teachers.measuring the blood pressure according to WHO,and sending the inquires papers on dangerous fact.Methods The civil servants and teachers in Dangling dis- trict were examined in October 2004.Results The result was made according to the statisitics analysis.It shows that occupation,sex,age,BMI,the history of smoking,the history of family.All these can cause the independent dan- gerous fact to the hypertensive disease.Conclusion Fat,high salt diet,smoking and drinking were all risk factors of high blood pressure.

Based on a retrospective review of researches on military stress home and abroad,this paper elaborates on the following4important issues:the promptness of selected literature,the advance of stress theories,the objectivity of the methods and the practicability of the results. [

Objective: To study various preparation methods for Tongmai Oral Liquid. Methods: Tongmai Oral Liquid with ultrafiltration method, water alcohol method, talcum powder method, and method were prepared respectively; using impurity clearing efficency, clarity, total saponin content as parameters for quality control. Results: Tongmai Oral Liquid prepared by water alcohol method (alcohol concentration being 75%) was better than that by other methods in claerity, and stability and has highest total saponin. Conclusions: Quality of Tongmai Oral Liquid prepared by water alcohol method was available.

Object To study the chemical constituents from leaves of Diplopanax stachyathus Hand Mazz aiming at searching for bioactive natural products Methods The compounds Ⅰ-Ⅹ were isolated by a combination of Sephedex LH 20 and silica gel column chromatographies, and their structures were identified by spectral methods Results Ten known compounds were isolated from leaves of D stachyathus Their structures were identified by means of spectroscopic analysis as: ? sitosterol (Ⅰ), betulinic acid (Ⅱ), oleanolic acid (Ⅲ), ursolic acid (Ⅳ), daucossterol (Ⅴ), quercetin 3 O ? D glucopyranoside (Ⅵ), 3,3′,4′ tri O methyellagic acid (Ⅶ), 3, 3′ di O methylellagic acid 4 O ? D xylopyranoside (Ⅷ), 3′ O methylellagic acid 4 O ? D xylopyranoside (Ⅸ), and 3, 3′,4′ tri O methylellagic acid O ? D glucopyranoside (Ⅹ) Conclusion Compounds Ⅰ-Ⅵ and Ⅸ are isolated from this plant for the first time

ObjectiveTo investigate the prevalence rate and risk factors of inpatients with coronary atherosclerotic heart disease (CHD) combined chronic kidney disease (CKD).MethodsAll people who underwent CHD combined CKD in CHD department of the First Affiliated Hospital of Xinjiang Medical University during January to December 2009 were enrolled in the retrospective study.Results A total of 960 hospitalized patients with CHD were enrolled during the observation period. The prevalence of proteinuria and reduced eGFR were 11.04％ and 10.52％,respectively.The total CKD prevalence rate was 16.77％,with male of 16.67％ and female of 17.11％.There was no significant difference in prevalence rate between male and female( P ＞ 0.05 ).The multi-factors logistic regression analysis showed that diabetes mellitus(OR 2.60,95％CI 1.17-3.29) was risk factor for CHD combined proteinuria.Ten-years older in age( OR1.55, 95％CI1.31-1.83 ), diabetesmellitus ( OR1.69, 95％CI1.15-2.47 ),hypercholesterolemia ( OR 2.89,95％ CI 1.49-5.61 ),and hyperuricemia( OR 1.49,95％ CI 0.96-2.33 )were risk factors for CHD combined CKD. Conclusion More attention should be paid to the high prevalence of CHD combined CKD.

Objective: To research the activity of antibacterial proteins isolated from Musca domestica during larvae-pupae metamorphosis. Methods: The 5-day-old larvae of Musca domestica were injured with a needle. 24h later, the larvae which had changed into pupae were selected. The antibacterial protein was isolated by a four-step protocol including grinding, heating, CM-sepharose F. F. cationexchange chromatography and another CM-sepharose F. F. chromatography. Antibacterial activities of samples were tested by an ultrasensitive radial diffusion assay using Staphylococcus aureus as the indicator organism. The molecular weight of the isolated protein was determined by SDS-PAGE.Results: The molecular weight of this isolated protein was 43kDa, and its antibacterial activity was strong. Conclusions: A kind of protein with strong antibacterial activity can be produced in the Musca domestica during larvae-pupae metamorphosis.

Objective To study the antileukemia effect of Carboxymethyl pachymaran (CMP) on p388 leukemia mice and its molecular mechanism. Methods After establishing leukemia normal model, they were randomly divided into groups and treated at the same time. FCM and mRNA in situ hybridization(ISH) and S- P assay immunocytochemistry were used for apoptosis and the expression of bcl- 2 mRNA and bcl- 2 protein in peripheral blood lymphocyte samples. Results CMP could prolong the span time of tumor- bearing mice 35.88 %, it was significant for CMP group compared with model group( P

[Objective]To study the effect of T cell groups of CD4+,CD8+,CD4+/CD8+,IL-4,IFN-? in the hypoimmunitymice induced by hydrocortisone to discuss its function of regulating the immune function and CD4+TH cell’s inferior change of group,have offered the experiment basis for clinical practice of TAF.[Method] FACS measured the T lymphocyte phenotype;the level of cytokine IFN-r and IL-4 was detected by ELISA.[Result] TAF can raise CD4+T lymphocyte of hypoimmunitymice,suppress CD8+ T lymphocyte expression,raise CD4+/CD8+ ratio;And can make the level of serum IFN-r obviously enhanced in hypoimmunitymice,and the level of IL-4 was very low.[Conclusion] TAF can rebuild immune function of hypoimmunitymice,promote organism Th0 to split up to Th1,maintain the advantage state of TH1,and strengthen the function of cellular mediated immunoresponse.