Objective To investigate the changes and clinical significance of cerebrospinal fluid (CSF) non-protein-bound iron and malondialdehyde(MDA) in preterm infants with severe intraventricular hemorrhage (IVH) after serial lumbar punctures.Methods Non-protein-bound iron and MDA of CSF in twenty severe IVH preterm infants with first and end lumbar puncture were examined respectively.The control samples were determined for once from first to second week after birth.Results Both Non-protein-bound iron and MDA levels of the first CSF sample in twenty severe IVH preterm infants were higher than that in control[(0.66±0.38)μmol/L vs (0.24±0.12)μmol/L(P<0.01);(1.21±0.41)μmol/L vs (0.89±0.35)μmol/L(P<0.05)].Non-protein-bound iron levels of first and end CSF sample in severe IVH with posthemorrhagic ventricular dilatation (PHVD) were both higher than those without PHVD [(0.75±0.34)μmol/L vs (0.54±0.31)μmol/L;(0.57±0.21)μmol/L vs (0.35±0.18)μmol/L](P<0.05).However,there was no significant difference in MDA levels between both groups.The levels of non-protein-bound iron of end CSF in severe IVH with and without PHVD were significantly lower than those of the first CSF samples (P<0.05),while,there was no significant difference in MDA levels between the end and first CSF samples.Conclusion The non-protein-bound iron and MDA of CSF in severe IVH preterm infants were both significantly increased,non-protein-bound iron and oxygen free radicals may be involved in the preterm brain injury.Serial lumbar punctures can lower the levels of non-protein-bound iron in CSF.

Objective To investigate clinical features,diagnosis and treatment of congenital adrenal hyperplasia (CAH) and to improve the knowledge of 21-hydroxylase deficiency form in CAH.Methods Ninteen neonatal CAH cases admitted to the neonatal intensive care unit of Shenzhen Children's Hospital from Jan 2004 to Aug 2011 were reviewed retrospectively.According to the early clinical manifestations,the serum levels of 17-hydroxyprogesterone,testosterone,progesterone,cortisol and adrenocorticotropic hormone (ACTH),early diagnosis was drawn and they were given to the glucocorticoid therapy.The alterations of blood sodium and potassium were compared before and after glucocorticoid therapy.Results Fourteen cases of 21-hydroxylase deficiency were salt-losing form who all were complicated with adrenal crisis and five cases were simple virilizing form.All CAH newborns(19 cases) appeared dark color of skin and mucosa.Fifteen cases had resistence to milk,poor response and feeding problem.The weight of eight cases at admission were lower than birth weight.Five cases suffered from repeating vomiting and diarrhea.After glucocorticoid therapy,there were significant improvement of hyponatremia[(110.2 ±9.5) mmol/L vs (138.7 ±5.2) mmol/L] and hyperkalemia[(7.40 ± 1.59) mmol/L vs (4.40 ±0.69) mmol/L] (P both ＜0.05).Conclusion We should raise the knowledge and level of diagnosis of neonatal CAH.We should diagnose the salt-losing form of 21 hydroxylase deficiency which is highly complicated with adrenal crisis through specific clinical manifestations and necessary biochemical assay as early as possible.The glucosteroid therapy is effective.

Objective To develop an advanced two-cuff technique orthotopic liver transplantation model in rats. Methods On the basis of the previous two-cuff-technique, some hypercoagulable blood was released from the recipient's PV and IVC before the cuffed donor PV and IVC were inserted into the lumen of the recipient's PV and IVC. The anastomosis of recipient's SVC was between the donor's diaphragm venaccaval hiatus and recipient's SVC. Results Every anastomosis was effective. The operative successful rate and one-week survival rate were 96.7?% and 90.0?% respectively. Conclusion This model could be used as an effective measure for the study of liver transplantation in rats.

Objective To evaluate the efficacy and safety of ultrasound-guided percutaneous ethanol injection(PEI)in the treatment of solitary benign thyroid adenoma.Methods One hundred and twelve patients with thyroid adenoma underwent PEI.The amount of anhydrous ethanol injected was approximately one third of the volume of thyroid nodule and used once a week,the injection was repeated two to five times.Results The average volume of pure adenoma and adenoma associated with cystic degeneration is ( 4.93 ? 0.48 )cm 3 and ( 7.89 ? 0.91 )cm 3;the nodules decreased markedly to ( 3.22 ? 0.40 )cm 3 and ( 3.85 ? 0.55 )cm 3 3 months after PEI treatment (P

Objective To study the clinical effects of low dose mifepristone together with methyltestosterone on the treatment of climacteric dysfunctional uterine bleeding(DUB).Methods Seventy patients with DUB were underwent dilatation and curettage of the uterus and divided into treatment group(n=35) and control group(n=35).The patients in the treatment group were treated with mifepristone and methyltestosterone,and those of the control group were treated with norlutin.The amenorrhea,the changes of endocrine and the pathology of endometrium were observed.Results All the patients were amenorrhea during the period of taking medicine and the regular menses was recovered after the treatment.Eight patients were menopause after the treatment.The levels of FSH,LH and P were decreased.The endometrium were atrophied and the anemia was improved.Conclusion Low dosage of mifepristone with methyltestosterone can prevent endometrial hyperplasis and it is safe and effective for the treatment of climacteric dysfunctional uterine bleeding.

The abnormal proliferation of vascular smooth muscle cells after endothe-lial injury is postulated to be the main pathophysiological process in atherosclerosis (AS). The effects of propylene glycol mannate sulfate(PGMS) on the proliferation of bovine cerebral microvessel smooth muscle cslls (BCMSMCs) induced by 10% fetal calf serum (FCS) and interleukin l(IL-1) were investigated in culture. 5 - 8 stage subcultured BCMSMCs were incubated into 96-well dish- With either 10% FCS or IL-1 (50u/ml) to produce BCMSMCs proliferation , the inhibitory effects of PGMS on proliferation of BCMSMCs were investigated. The results shows that PGMS could inhibit the proliferation of quiescent BCMSMCs induced by 10% PCS. The growth of cells was inhibited, comparing with normal control 72hours after the serum addition as determined by crystal violet stainning and MTTmethod. The proliferation of quiescent BCMSMCs induced by IL-1 (50u/ml) was also inhibited by PGMS as determined by crystal violet stainning and MTT method. The results suggested that PGMS inhibit the proliferation of BCMSMCs induced by 10% FCS and IL-1 ,and the use of PGMS may probably play an important role in the treatment of cerebrovascular disease.

The effects of propylene glycol mannate sulfate (PGMS) on the lipid peroxide (MDA) , superoxide dismutase (SOD) , glutathion peroxidase (GSH-Px), nitric oxide (NO)and water content in the whole cerebral ischemia/reperfusion injury rabbits induced by four-vessel occlusion,The results show that PGMS can decrease the brain water and MDA, and can increase the SOD and GSH-Px level. No significant effect on the NO level has been detected. The results suggest that the protective effects of PGMS on ischemia/reperfusion injury may be related to its antioxidation.

OBJECTIVE To study the quality of the related articles published in a core biomedical journal Chinese Journal of Nosocomiology in the latest five years. METHODS Statistical analysis and quality evaluation were performed based on the 2 612 related papers which selected from its 48 issues published during the period from 2000 to 2004. RESULTS(1) Five year average publication capacity index was 0.57.(2) The majority authors were from medical universities or their affiliated hospitals and provincial or military medical units.(3) The majority types of the papers were scientific research articles,theses and reviews.(4) There were 160 grant based projects.(5) The average quotations were 6.0 in each paper,among them: 68.6% were Chinese literature,31.4% were from foreign sources,while 34.2% were self-citation.(6) The average issues time lag was 273.5 days in the latest five years. CONCLUSIONS Chinese Journal of Nosocomiology has the superiority in the above aspects,which confirmed its quality as a core journal.

Objective To investigate the effects of Yun-Pi Prescription on hemolysin antibody,and approach its mechanism of regulating immune function.Methods Forty-eight KM mice were randomly divided into high,medium and low dose of Yun-Pi Prescription groups,which were given 20,10,5 mL/kg Yun-Pi Prescription respectively,and one control group with no intervention.From the seventh day,all groups were injected with chicken red blood cell for seven days.Levels of serum hemolytic optical density were measured in all groups.Results Compared with control group,levels of serum hemolytic optical density in Yun-Pi Prescription groups increased significantly(P

Objective To study the signal transduction pathway of norepinephrine (NE)-induced expressions of oncogenes c-fos and c-myc in the rat heart. Methods mRNA levels of c-fos and c-myc in the left ventricle of the Langendorff-perfused rat heart was quantitated by reverse transcription and polymerase chain reaction (RT-PCR). Results NE increased the expressions of c-fos and c-myc while the effects of NE on the expressions of c-fos and c-myc were blocked by ? 1-adrenergic blocker prazosin (5?10 -6 mol/L) completely. Neomycin (5 mmol/L) and ruthenium red (10 ?mol/L) decreased the NE-induced c-fos and c-myc mRNA levels. Conclusion NE can induce the expressions of oncogenes c-fos and c-myc in the ventricle mediated by ? 1-adrenergic receptor. The signal transduction pathway of NE-induced c-fos and c-myc expressions may be associated with inositol phosphates-Ca 2+ system and diacylglycerol-protein kinase C system.