To investigate the mechanism of Xiefei Dingchuan injection (XFDCI,泻肺定喘注射液) in treating pulmonary hypertension by means of measuring the activity of Ca2＋Mg2＋ ATPase and Na＋K＋ATPase of red blood cell(RBC) membrane.Methods:The pulmonary hypertension models induced by monocrotaline in 18 rats were established then treated with XFDCI.Using cardiac catheter and chromatometry respectively the pulmonary artery pressure (PAP) and the activities of Ca2＋Mg2＋ATPase and Na＋K＋ATPase of RBC membrane were measured.Results:The activities of Ca2＋Mg2＋ATPase and Na＋K＋ATPase of RBC membrane in XFDCI group were obviously higher than those in pulmonary hypertension group.Conclusions:XFDCI is able to reduce the pulmonary hypertension and its mechanisms may be related to increasing the activities of Ca2＋Mg2＋ATPase and Na＋K＋ATPase of RBC membrane.

Rapid development of personalized medicine in cancer treatments needs urgent support of proper tumor biomarkers.Comparing to tissue tests,serum/plasma tumor biomarkers show advantages in easy sample collection and flexibility of detecting methods.Nowadays,large scales of investigations have discovered sufficient blood tumor biomarkers for preclinical diagnosis and evaluation of clinical treatment or prognosis,with the development of screening techniques and strategies.Moreover,tremendous promotion would appear in cancer diagnosis and treatment with future profound investigation and rigorous verification from clinical practice.

Objective To investigate the effect of community nursing intervention on hypertension combined with diabetes in elderly patients.Methods Five hundred and twenty elderly hypertensive patients with diabetes aged over 60 years in 10 communities of Shenzhen were randomly divided into a control group and a study group,260 cases in each group.All the patients in the two groups were given regular follow-ups in community clinics after discharge.Then the control group received instructions on relevant knowledge by the nurses in the community clinics,while the study group received nursing intervention by phone calls or visits by professional nurses once a month.The two groups were compared in terms of blood pressure,blood sugar and blood lipids 12 months after intervention.Results There were no significant differences in blood pressure,blood sugar and blood lipids between the two groups before the intervention(all P>0.05).After intervention,however,the differences were significant(all P<0.05).Conclusion Community nursing intervention may effectively improve hypertension and diabetes in the elderly patients by improving the levels of blood pressure,blood glucose and blood lipid,thereby reducing the incidence of cardiovascular and cerebrovascular events.

Objective To establish the healthcare management mode for pregnant women adapting to the characteristics of transient population which can safeguard the maternal and infant health or reduce the maternal mortality rate and prenatal mortality rate. Methods Five suburban communities of Chaoyang District, Haidian District and Fengtai District of Beijing were chosen as intervention group, which received systemic healthcare management of pregnant women in transient population, another five suburban communities were selected as control group. Results Both maternal mortality rate and prenatal mortality rate in intervention group were lower than those in control group. To evaluate the effect of systemic healthcare for pregnant women, an average of four antenatal clinic visits were completed in intervention group and eight visits in control group. There was no statistical difference in occurrence of maternal complications, newborn weight and occurrence rate of neonatal asphyxia between two groups. The expense of hospital vaginal delivery was no more than 1000 yuan in intervention group and more than 1000 yuan in control group, while the outcomes of mothers and neonates in two groups had no significant difference. Conclusion The systemic healthcare and community management mode for pregnant women in transient population can safeguard the maternal and infant health with scientific, practical, and replica-ble significances.

Objective To study the effect of Qi-Gong relaxation training on the operation-related psychological stress and the post-operative psychosomatic adjustment in upper-abdominal surgery patients. Methods Forty-two patients undergoing upper-abdominal surgery in intervention group were instructed to do Qi-Gong relaxation training, and forty patients matched were controls without Qi-Gong relaxation training. On the day before surgery, a self-developed patients' pre-operative appraisal questionnaire, a self-developed patients' pre-operative behavior rating questionnaire for completion by medical workers, and State Anxiety Inventory (SAI) of State -Trait Anxiety Inventory (STAI) were used in two groups. In an hour before operation，the shift of the mean value of pulse and systolic pressure of every case compared with the base line measured at his (or her) admission was also recorded. After surgery, patients were assessed with a self-developed patients' post-operative appraisal questionnaire, a self-developed patients' post-operative behavior rating questionnaire for completion by medical workers and SAI of STAI. Besides, the indexes of physical rehabilitation, including intestinal aerofluxus, total dosage of analgesia and so on, were recorded. Data were analysed in t-test with SPSS. Results Compared with controls, the patients in intervention group had more positive anticipation about the following operation, lower pre- and post-operative anxiety, and lighter post-operative pain experience. There was no difference in the indexes of physical rehabilitation between two groups. Conclusions Qi-Gong relaxation training could lead patients to better psychological adjustment both before and after operation. This finding suggests that Qi-Gong relaxation training could be used as an effective psycho-behavior intervention in abdominal surgery patients.

As the precision medicine has been proposed , personalized therapy in cancer steps into a new stage.Since the development of gene molecular biology in lung cancer , numerous molecular targets were discovered.However, selecting patients who will be the most likely to benefit from the therapy is still a great challenge in clinical practice .Companion diagnosis could help evaluate the status of patients , which may provide better suggestions for health care professionals during treatment decision , in order to benefit the patients and decline the side effects risk together .Besides, high-throughput sequencing technics and diverse detection samples has provided a novel direction to understand the tumorigenesis and find more potential gene targets.

Objective To investigate the relationship between adenosine triphosphate-binding cassette superfamily G member 2 (ABCG2) mRNA and protein expressions and chemotherapeutic efficacy and drug resistance in breast cancer patients. Methods 24 patients with breast cancer were selected as study subjects, all patients were treated with anthracycline chemotherapy after chemotherapy, according to WHO criteria, the patients were divided into effective group, stable group and advanced group according to chemotherapy effect. The levels of ABCG2 mRNA and protein in the breast tissue were detected by qPCR and ELISA before and after chemotherapy. Results After chemotherapy, the total effective rate was 41.67%, 58.33% of patients still developed drug resistance. The expressions of ABCG2 mRNA and protein in patients after chemotherapy were significantly higher than those before chemotherapy (P<0.05). The expressions of ABCG2 mRNA and protein were significantly lower in the effective group than in the stable group and advanced group (P<0.05). The expressions of ABCG2 mRNA and protein in the stable group was significantly lower than that in the advanced group (P<0.05). Conclusion The expression level of ABCG2 was correlated with the chemotherapeutic efficacy and drug resistance of breast cancer patients. The higher the ABCG2 expression level, the stronger the ability of promoting the excretion of drugs, the more significant the drug resistance of tumor cells and the worsen the chemotherapy effect.

Objective:To investigate the expression of TLR4 mRNA?TLR2 mRNA in the peripheral blood mononuclear cells (PBMC) from the patients with Gram positive and Gram negative infections.Methods:TLR4 and TLR2 mRNA were detected in the patients with Gram negative infections ( n =20) and the patients with Gram positive infections ( n =15) and the normal persons as control ( n =24) with the method of Taqman Real-time PCR.Results:TLR4 mRNA expression in the patients with Gram negative infections were significantly higher than Gram positive infections patients and the normals ( P 0.05).Conclusion:The results showed that the increased expression levels of TLR4 mRNA in PBMC from the Gram negative infectious patients are associated with Gram negative infections and the detection of TLR4 mRNA expression may be a method of diagnosis for Gram negative infections in the early stage.

The effects of L-arginine (L-Arg), the physiological NO precureor and NO synthetase inhibitor NG-nitro-L-arginine (L-NNA) on endogenous endothelin (ET) secretion and renal function were observed in a model of glycerol-induced acute renal failure (ARF) in rat. It was found that endogenous ET secretion was significantly increased, while serum NO was markedly decreased in ARF rats. The administration of L-NNA to the ARF rats induced significant increases in plasma ET and positive immunoreactive particles of ET in renal tubular epithelial cells(EP cells), and the impairment of renal function was exaggerated. L-Arg might effectively decrease the level of plasma ET and the positive immunoreactive particles of ET in renal tubular EP cells, suggesting the improvement of the renal function. It is suggested that the ability of NO in improving renal function may relatedto the inhibition of endogenous ET secretion in glycerol-induced ARF rats.