Epithelial-mesenchymal transition is closely related with invasion and metastasis of malig-nant tumor and is focused on invasion and metastasis in malignant tumor in recent years .Many researches dermon-strate that epithelial-mesenchymal transition is involved in invasion and metastasis of several malignant tumors , such as breast cancer ,ovary cancer and non -small cell lung cancer .The relevant signal pathways and molecular proteins are also studied .Research progress of epithelial -mesenchymal transition in non -small cell lung cancer for invasion and metastasis is reviewed in this article .

Objective To investigate the change of peripheral blooe natural killer(NK)cell subsets of patients with recurrent spontaneous abortion( RSA),in oreer to proviee the relevant scientific evieence for unexplainee recurrent spontaneous abortion( URSA) eiagnosis ane treatment. Methods Sixty-three RSA patients were raneomly eivieee into CRSA(n = 33)ane URSA(n = 30). Meanwhile 30 cases normal pregnancy women were selectee as control. Flow cytometry was appliee to eetect NK cell levels in patients with RSA ane its subsets percentage,ane then comparee to the control group. Results NK cells subset in RSA,URAS ane normal pregnancy women were(13. 77 ± 1. 53)% ,(13. 97 ± 1. 60)% ,ane(13. 65 ± 1. 74)%. There was no significant eifference among three groups( F = 0. 287,P > 0. 05). The percent of CD56 + CD16 - ,CD56 +CD16 + ,CD56 - CD16 + NK cell subsets in RAS group were:(70. 00 ± 2. 49)% ,(13. 76 ± 2. 13)% ,(7. 04 ± 1. 44)% respectively,ane(56. 50 ± 3. 94)% ,(22. 88 ± 3. 22)% ,(11. 24 ± 2. 21)% in URAS group, meanwhile(70. 48 ± 3. 21)% ,(14. 16 ± 2. 14)% ,(6. 53 ± 1. 98)% in normal pregnancy group. There was no significant eifference between RSA ane normal pregnancy group( P > 0. 05). However,There was markeely eifference between URSA with RSA or normal group(F = 182. 587,18. 118,58. 879,P < 0. 05). Conclusion NK cell activity function plays a key role in the eevelopment of URSA. The eetection of the percentage of NK cell subsets of women euring pregnancy will help RSA monitoring ane NK cell subsets can preeict the percentage change in the pregnancy outcome .

Objective To discuss the treatment effect of doctor-nurse cooperation in multi-plane operation of obstructive sleep apnea hypopnea syndrome.Methods 73 patients with multi-planar surgery due to OSAHS were divided into the observation group (38 cases) and the control group(35 cases) in our department from January 2008 to December 2012.Health care was strengthened throughout the course of treatment in the observation group and traditional treatment and care were adopted in the control group.Short-term and long-term efficacy,intraoperative and postoperative complications were compared between two groups.Results The operation of short-term effective rate was 100％,and significant differences were found in the long-term efficacy between the two groups.There were significant differences in nasal bleeding during surgery,one-time intubation success rate and the significances were found in postoperative pain,extubation time,lung infection and soft palate regurgitation between two groups.Conclusions The implementation of the multi-plane surgery for patients with OSAHS can effectively improve the short-term efficacy of the patients throughout the course of treatment.Strengthening doctor-nurse cooperation effectively improve the patients' long-term efficacy,reducing the patients' intraoperative and postoperative complications.

Objective To explore the causes,prevention and treatment of common complications in stone extraction by choledochoscopy after bile duct operation.Methods The clinical data of 165 cases with complications after stone extraction by choledochoscopy in 1379 treated cases during 7 years were analyzed.Results The common unfavorable reactions of nausea,vomiting,abdominal pain and diarrhea occurred in 107 cases,and were alleviated by controlling the amount and rapidity of the infused sterilized water during choledochoscopy.Chills and fever occurred in 25 cases and were controlled by adequate T tube drainage and antibiotics.Bile duct hemorrhage occurred in 11 cases,including automatic hemostasis in 5 cases,hemostasis after infusing thrombin by T tube in 5 cases and hemostasis after infusing thrombin by vein in 1 case.Biliary fistula from sinus tract perforation occurred in 3 cases,and in these,T tube was successtully re-inserted in 1 case and T tube was inserted by reoperation in 2 cases.T-tube tract could not admit the choledochoscope in 19 cases,and were treated by re-choledochoscopy after gradual T-tube dilataton in 12 cases,but the sinus tract close to common bile duct was completely obstructed in 7 cases.Conclusions Choledochoscopic removal of retained stones is a safe procedure but unfavorable reactions and complications are not uncommon.The severe complications,such as bile duct hemorrhage and biliary fistula,should be prevented and appropriately treated.

Objective To study the clinical effects of low dose mifepristone together with methyltestosterone on the treatment of climacteric dysfunctional uterine bleeding(DUB).Methods Seventy patients with DUB were underwent dilatation and curettage of the uterus and divided into treatment group(n=35) and control group(n=35).The patients in the treatment group were treated with mifepristone and methyltestosterone,and those of the control group were treated with norlutin.The amenorrhea,the changes of endocrine and the pathology of endometrium were observed.Results All the patients were amenorrhea during the period of taking medicine and the regular menses was recovered after the treatment.Eight patients were menopause after the treatment.The levels of FSH,LH and P were decreased.The endometrium were atrophied and the anemia was improved.Conclusion Low dosage of mifepristone with methyltestosterone can prevent endometrial hyperplasis and it is safe and effective for the treatment of climacteric dysfunctional uterine bleeding.

Objective]To observe the clinical effect of Yinlai Decoction Addition and Subtraction on treating Pediatric Cold about lung-stomach heat retention syndrome.[Method]This study adopted the research design method of case series reports, continuously included 40 Pediatric Cold children with lung-stomach heat retention syndrome treated by Yinlai Decoction Addition and Subtraction from November 2011 to December 2012. Through clinical visiting, we could assess the treatment outcome of this time on the basis of evaluation standard. [Results]33 patients were cured, 5 patients turned better and 5 patients had no effect, the total effective rate was 95.00%. [Conclusion]Yinlai Decoction had clearing and dispersing function, it could treat lung and stomach simultaneously, therefore, it had obvious efficacy in Pediatric Cold about lung-stomach heat retention syndrome.

Objective To study the relation between chronic stressful, the neural changes in prefrontal cortex and depression. Methods Adapt chronic unpredictable stress with separate model to make depression model rats. After 22 days all the rats were killed and use immunohistochemistry method and computer image analysis to detect BDNF. To analysis the date with SPSS11.5 software. Results After 21 days stress, body weight ( t =2.915, P < 0.05), ambulation ( t = 6. 245, P < 0. 01 ), rearing( t = 2.693, P < 0. 05 ) and grooming ( t = 2. 685, P<0.05) decreased and stopping time in center( t=2. 388, P<0. 05) ,defecation( t =3. 846, P<0. 01 ) increased in experimental group. BDNF expressed obviously in control group and the prefrontal cortex expressed highly than that of the experimental group. BDNF expressions of experimental group were lower than that in control group ( P< 0.01 ) especially in right prefrontal cortex. Conclusion There was no difference of BDNF distribution in prefrontal cortex between both groups ,but after 21 days stress ,the BDNF levels of experimental rats obviously descent,especially in right prefrontal cortex.

It is a clinical nursing management that can be adapted to chinese characteristic and the market ecnomic body.We studied 194 cases of patients nursed by 3-level nursing ward attendance using nursing procedure, and compared itwith the traditional way. Reault: The beneficial indicator of the study group was significantly higher than that of thetraditional group. 3 - level hursing ward attendance using nursing procedure is a scientific nursing management way. Itembodied the patients- centralized - nursing - management mode. The knoweldge and application of nursing managementwere also developed and increased

Objective To investigate the Survivin and Cyclooxygenase-2 (COX-2) expression and their relationship in cervical carcinoma tissues and to investigate the relationship with the genesis and devclopment of cervical carcinoma.Methods The expression of Survivin and COX-2 was assayed by immunohistochemical technique in 60 cervical carcinoma and 30 normal cervical tissue specimens.The relation with its clinical pathology characters were analysed.Results Survivin and COX-2 expression in the tissues of cervical carcinoma [76.7 ％ (46/60),63.3 ％ (38/60)] were higher than that in normal cervical tissues evidently [6.7 ％ (2/30),10.0 ％ (3/30)] (P ＜ 0.05).The COX-2 expression in cervical carcinoma was positively associated with lymph node metastasis (P ＜ 0.05),but no correlation was found between COX-2 expression and age,clinical stages,grades and invasive depth (P ＞ 0.05).Any correlation between Survivin expression and classical prognostic factors of cervical carcinoma was found.Moreover,these two proteins were positively correlated.Conclusion The Survivin and COX-2 might play important roles in the development of cervical carcinoma.

Objective To study the action of enantone in chocolate cyst after laparoscopic surgery. Methods To devide the 76 patients into two groups, study group(50 patients, additional enantone after laparoscopic surgery) and the control group (26 patients, only laparoscopic surgery), to analyse their recurrence rate and pregnancy rate. Results The recurrence rate of study group was obviously lower than control group. In the 48 patients with infertility, the pregnancy rate of study group was higher than control group. Conclusions In chocolate cyst after laparoscopic surgery, enantone can decrease the recurrence rate and increase pregnancy rate.