1.Research progress of epithelial-mesenchymal transition in Non-small cell lung cancer invasion and me-tastasis
Practical Oncology Journal 2013;(6):559-562
Epithelial-mesenchymal transition is closely related with invasion and metastasis of malig-nant tumor and is focused on invasion and metastasis in malignant tumor in recent years .Many researches dermon-strate that epithelial-mesenchymal transition is involved in invasion and metastasis of several malignant tumors , such as breast cancer ,ovary cancer and non -small cell lung cancer .The relevant signal pathways and molecular proteins are also studied .Research progress of epithelial -mesenchymal transition in non -small cell lung cancer for invasion and metastasis is reviewed in this article .
2.The clinical significance of detecting peripheral blood natural killer cell in the unexplained recurrent spontaneous abortion
Clinical Medicine of China 2014;(6):574-576
Objective To investigate the change of peripheral blooe natural killer(NK)cell subsets of patients with recurrent spontaneous abortion( RSA),in oreer to proviee the relevant scientific evieence for unexplainee recurrent spontaneous abortion( URSA) eiagnosis ane treatment. Methods Sixty-three RSA patients were raneomly eivieee into CRSA(n = 33)ane URSA(n = 30). Meanwhile 30 cases normal pregnancy women were selectee as control. Flow cytometry was appliee to eetect NK cell levels in patients with RSA ane its subsets percentage,ane then comparee to the control group. Results NK cells subset in RSA,URAS ane normal pregnancy women were(13. 77 ± 1. 53)% ,(13. 97 ± 1. 60)% ,ane(13. 65 ± 1. 74)%. There was no significant eifference among three groups( F = 0. 287,P > 0. 05). The percent of CD56 + CD16 - ,CD56 +CD16 + ,CD56 - CD16 + NK cell subsets in RAS group were:(70. 00 ± 2. 49)% ,(13. 76 ± 2. 13)% ,(7. 04 ± 1. 44)% respectively,ane(56. 50 ± 3. 94)% ,(22. 88 ± 3. 22)% ,(11. 24 ± 2. 21)% in URAS group, meanwhile(70. 48 ± 3. 21)% ,(14. 16 ± 2. 14)% ,(6. 53 ± 1. 98)% in normal pregnancy group. There was no significant eifference between RSA ane normal pregnancy group( P > 0. 05). However,There was markeely eifference between URSA with RSA or normal group(F = 182. 587,18. 118,58. 879,P < 0. 05). Conclusion NK cell activity function plays a key role in the eevelopment of URSA. The eetection of the percentage of NK cell subsets of women euring pregnancy will help RSA monitoring ane NK cell subsets can preeict the percentage change in the pregnancy outcome .
3.Effect of doctor-nurse cooperation in multi-plane operation of obstructive sleep apnea hypopnea syndrome
Chinese Journal of Practical Nursing 2013;29(20):17-19
Objective To discuss the treatment effect of doctor-nurse cooperation in multi-plane operation of obstructive sleep apnea hypopnea syndrome.Methods 73 patients with multi-planar surgery due to OSAHS were divided into the observation group (38 cases) and the control group(35 cases) in our department from January 2008 to December 2012.Health care was strengthened throughout the course of treatment in the observation group and traditional treatment and care were adopted in the control group.Short-term and long-term efficacy,intraoperative and postoperative complications were compared between two groups.Results The operation of short-term effective rate was 100%,and significant differences were found in the long-term efficacy between the two groups.There were significant differences in nasal bleeding during surgery,one-time intubation success rate and the significances were found in postoperative pain,extubation time,lung infection and soft palate regurgitation between two groups.Conclusions The implementation of the multi-plane surgery for patients with OSAHS can effectively improve the short-term efficacy of the patients throughout the course of treatment.Strengthening doctor-nurse cooperation effectively improve the patients' long-term efficacy,reducing the patients' intraoperative and postoperative complications.
4.Expression of Survivin and cyclooxygenase-2 and their relationship in cervical carcinoma
Jing HE ; Jieqiang LYU ; Xiaohong HOU
Cancer Research and Clinic 2012;24(8):518-520
Objective To investigate the Survivin and Cyclooxygenase-2 (COX-2) expression and their relationship in cervical carcinoma tissues and to investigate the relationship with the genesis and devclopment of cervical carcinoma.Methods The expression of Survivin and COX-2 was assayed by immunohistochemical technique in 60 cervical carcinoma and 30 normal cervical tissue specimens.The relation with its clinical pathology characters were analysed.Results Survivin and COX-2 expression in the tissues of cervical carcinoma [76.7 % (46/60),63.3 % (38/60)] were higher than that in normal cervical tissues evidently [6.7 % (2/30),10.0 % (3/30)] (P < 0.05).The COX-2 expression in cervical carcinoma was positively associated with lymph node metastasis (P < 0.05),but no correlation was found between COX-2 expression and age,clinical stages,grades and invasive depth (P > 0.05).Any correlation between Survivin expression and classical prognostic factors of cervical carcinoma was found.Moreover,these two proteins were positively correlated.Conclusion The Survivin and COX-2 might play important roles in the development of cervical carcinoma.
5.Risk factors of premature behavioral neurological assessment
Xiaohong HUANG ; Juan HE ; Xiaowen CHEN
Chinese Pediatric Emergency Medicine 2011;18(2):116-119
Objective To analyze the characteristic of twenty-item premature behavioral neurological assessment,and to assess the risk factors of neonatal behavioral neurological assessment(NBNA) in premature infants. Methods Total two hundred and seventy-four premature infants hospitalized in Guangzhou Children's Hospital were assessed with the NBNA after correcting age 40 weeks,premature infants were divided into two groups:normal group (n =221 ) and abnormal group (n =53) according to NBNA. The birth history, mother pregnancy situation, the disease during neonatal period and results in hospital were reviewed,and the risk factors of NBNA in premature infants were assessed. Results There were significant differences in gestational age, birth weight, head circumference, body height, Apgar score, resuscitation time, hospitalization time and total gastrointestinal nutrition time between the two groups( P < 0. 05 ). The incidences of superfoetation,hyaline membrane disease, hemorrhagic and hypoxic brain injury, severe infection, hyperbilirubinemia, hypoglycemia and chronic lung disease were different between the two groups( P < 0. 05 ). There were no differences in gender, antenatal hormone use, anaemia, hypotension and evoked potential abnormality (P >0. 05 ). Multivariate logistic regression analysis showed that the risk factors of NBNA score in premature infants would include low Apgar score, low birth weight, hyaline membrane disease, hemorrhagic and hypoxic brain injury, severe infection, hyperbilirubinemia, hypoglycemia and chronic lung disease. Conclusion Although the premature infants corrected age 40 weeks,but the capability and initiative muscular tension are still lower than term infant. Low Apgar score, low birth weight, hyaline membrane disease, hemorrhagic and hypoxic brain injury ,severe infection ,hyperbilirubinemia,hypoglycemia and chronic lung disease are important risk factors related to premature behavioral neurological assessment.
6.The impact of chronic stress on the behaviors and the expression of brain-derived neurotrophic factor in the prefrontal cortex of rats
Ru HE ; Yun XIAO ; Xiaohong FENG
Chinese Journal of Behavioral Medicine and Brain Science 2008;17(6):523-524
Objective To study the relation between chronic stressful, the neural changes in prefrontal cortex and depression. Methods Adapt chronic unpredictable stress with separate model to make depression model rats. After 22 days all the rats were killed and use immunohistochemistry method and computer image analysis to detect BDNF. To analysis the date with SPSS11.5 software. Results After 21 days stress, body weight ( t =2.915, P < 0.05), ambulation ( t = 6. 245, P < 0. 01 ), rearing( t = 2.693, P < 0. 05 ) and grooming ( t = 2. 685, P<0.05) decreased and stopping time in center( t=2. 388, P<0. 05) ,defecation( t =3. 846, P<0. 01 ) increased in experimental group. BDNF expressed obviously in control group and the prefrontal cortex expressed highly than that of the experimental group. BDNF expressions of experimental group were lower than that in control group ( P< 0.01 ) especially in right prefrontal cortex. Conclusion There was no difference of BDNF distribution in prefrontal cortex between both groups ,but after 21 days stress ,the BDNF levels of experimental rats obviously descent,especially in right prefrontal cortex.
7.Investigation of 3 - Level Nursing Ward Attendance Using Nursing Procedure
Xiaohong GUO ; Qiaoying GUO ; Chisheng HE
Chinese Journal of Practical Nursing 2001;17(3):49-50
It is a clinical nursing management that can be adapted to chinese characteristic and the market ecnomic body.We studied 194 cases of patients nursed by 3-level nursing ward attendance using nursing procedure, and compared itwith the traditional way. Reault: The beneficial indicator of the study group was significantly higher than that of thetraditional group. 3 - level hursing ward attendance using nursing procedure is a scientific nursing management way. Itembodied the patients- centralized - nursing - management mode. The knoweldge and application of nursing managementwere also developed and increased
8.Case Series Reports of 40 Pediatric Cold Children with Lung-stomach Heat Retention Syndrome Treated by Yinlai Decoction Addition and Subtraction
Guokai LV ; He YU ; Xiaohong GU
Journal of Zhejiang Chinese Medical University 2014;(8):973-975
Objective]To observe the clinical effect of Yinlai Decoction Addition and Subtraction on treating Pediatric Cold about lung-stomach heat retention syndrome.[Method]This study adopted the research design method of case series reports, continuously included 40 Pediatric Cold children with lung-stomach heat retention syndrome treated by Yinlai Decoction Addition and Subtraction from November 2011 to December 2012. Through clinical visiting, we could assess the treatment outcome of this time on the basis of evaluation standard. [Results]33 patients were cured, 5 patients turned better and 5 patients had no effect, the total effective rate was 95.00%. [Conclusion]Yinlai Decoction had clearing and dispersing function, it could treat lung and stomach simultaneously, therefore, it had obvious efficacy in Pediatric Cold about lung-stomach heat retention syndrome.
9.Therapeutic Effect of Guishen Pill Combined with Acupuncture for Male Immune Infertility and Influence on Acid Phosphatase in Seminal Plasma
Yanping HE ; Yang SONG ; Xiaohong ZHANG
Journal of Guangzhou University of Traditional Chinese Medicine 2004;0(05):-
【Objective】To observe therapeutic effect of Guishen Pill(GP) combined with acupuncture(AP) for male immune infertility(MII) and to investigate influence on acid phosphatase(ACP) in seminal plasma.【Methods】One hundred and five MII patients were equally randomized into three groups.Group A received GP combined AP,group B received GP and group C received prednisone.The therapeutic effect was evaluated in the three groups after treatment,and the changes of ACP were observed before and after treatment.【Results】The total effective rate was 91.43%,88.57% and 68.57%,and the cure rate was 45.71%,22.86% and 11.43% in groups A,B and C respectively.The difference of total effective rate was significant in groups A and B as compared with that in group C(P
10.Changes of IL-2, TNF, TGF and IL-2R mRNA of Menopausal Rats and Effects of Preventive Acupuncture on Guanyuan Point
Yuwei HE ; Xiaohong LI ; Lufen ZHANG
Chinese Journal of Information on Traditional Chinese Medicine 2006;0(04):-
Objective To observe the effect on IL-2,TNF,TGF in uterus and serum,and IL-2RmRNA in spleen of menopausal rats with preventive accupuncture on Guanyuan point,and analysis the mechanism.Methods 112 ten month-aged SD female rats examined by vaginal smear were chosen and divided into seven groups.And another 16 three point five month-aged SD female rats were another group.Radio-immunity and hybridiztion in situ methods were applied to observe the effect on IL-2,TNF,TGF in uterus and serum and IL-2R mRNA in spleen of 12,14 and 16 month-aged menopausal rats with preventive accupuncture on Guanyuan point(twice a week,eight weeks continued) in 10th month.Results Compared with the normal group,IL-2,TGF in uterus and serum and TNF in uterus of menopausal rats reduced,TNF in serum came down in the 12th month and went up in 14th and 16th month,IL-2R mRNA in spleen decreased.Compared with the same-aged model group,IL-2,TGF of serum and uterus and TNF in uterus increased.TGF in serum of 14 and 16 month-aged group,IL-2 in uterus of 12 and 16 month-aged group,TNF in uterus of 12 month-aged group and TGF in uterus of 12 and 16 month-aged group rised significantly(P