BACKGROUND: Several studies have demonstrated that degradable polycaprolactone film has no apparent influences on cellular immune function of rat critical organs including heart, liver, and kidney, and possesses the prerequisite of anti-adhesion formation.OBJECTIVE: To investigate the role of degradable polycaprolactone film in preventing rat peritoneal adhesion following surgery.DESIGN, TIME AND SETTING: The present randomized, controlled animal experiment was performed at the Department of Physiology, Jilin University.MATERIALS: A total of 120 male Wistar rats, weighing 180-220 g, were included in this study. Peritoneal adhesion was induced in all rats. Non-bio-degradable film was sourced from Wuhan Boster Company, China. Polycaprolactone film was purchased from U.C.C Company. England.METHODS: All 120 rats were randomly divided into 3 groups, with 40 rats per group: control, non-bio-degradable film, and polycaprolactone film. Peritoneal adhesion was induced in all rats. The non-bio-degradahle film and polycaprolactone film groups were subjected to peritoneal wound cover with non-bio-degradable film and polycaprolactone film, respectively. From day 1 following surgery, each rat daily received an intraperitoneal injection of 4 mL 2 g/L pseudomonas enzyme, for a total of 3 successive days.MAIN OUTCOME MEASURES: At 1, 3, 7, and 30 days following surgery, 10 rats were selected from each group for observation of peritoneal adhesion.RESULTS: Totally 120 rats were included in the result analysis. Peritoneal adhesion was successfully induced in rats from the control group. From day 7 following surgery, the peritoneal adhesion rate was significantly lower in the polycaprolactone film group than in the control group (P<0.01). In the non-bio-degradable film group, the peritoneal adhesion rate exhibited decreasing followed by increasing tendency; by day 30, the adhesion rate was significantly higher compared to the polycaprolactone film group (P<0.01).CONCLUSION: Polycaprolactone film is degraded by pseudomonas enzyme to avoid the new adhesion caused by foreign stimulation.

Objective To investigate the effects of high molecular bio-degradable film on preventing peritoneal adhesion.Methods One hundred and twenty rats were divided into control group,non-bio-degradable film group and polycaprolactone(PCL) group.Animal models of peritoneal adhesion made by operations on rats serve as control group.In the study,non-bio-degradable film and bio-degradable,namely PCL films sensitive to pseudomonas enzyme,were put into the opening of peritoneum.The effect of the films on peritoneal adhesion was examined on 1,3,7 and 30 d after operation.Results Peritoneal adhesion was found in all the rats of control group and very low in PCL group and non-bio-degradable film group.Conclusion PCL film can effectively prevent the formation of postoperative peritoneal adhesion.

Objective To determine the relationship of bone mineral density (BMD) with age and sex hormone in elderly men. Methods The BMD in orthotope lumbar vertebra, femur neck, greater trochanter, Wards triangle region and shaft of femur were measured by dual-energy X-ray absorptiometry (DEXA), and serum testosterone (TT) and estradiol (E2) were determined by chemiluminescence in 360 elderly men. According to age and BMD, the elderly men were divided into several groups. The differences in BMD, serum TT and E2 among the groups were compared. Results There were 48 cases in osteoporosis group, 72 cases in bone mass reduction group and 240 cases in normal control group. The BMD in femur neck, greater trochanter of femur, Wards triangle region and shaft of femoral were decreased with ageing (F=3. 038,3. 029,3. 024,3. 021, respective, all P<0.05). In>80 age group, the BMD in femur neck, greater trochanter of femur, Wards triangle region and shaft of femoral were (0. 70±0. 140), (0. 682±0. 185), (0. 629±0.211), (0. 986± 0. 160)g/cm2, respectively;In 70-80 age group, they were (0. 829±0. 156), (0. 765±0. 170), (0. 698±0.187), (1. 042±0. 190) g/cm2, respectively;In 60-70 age group, they were (0. 875± 0. 138), (0. 800±0. 130), (0. 731±0. 145), (1. 071±0. 125)g/cm2, respectively. The differences in BMD of L1-4 had no statistical significance among different age groups(F=2. 988, P>0.05). There was no difference in level of serum TT among normal control group, bone mass reduction group an dosteoporosis group (F=3. 032, P>0. 05), while the level of serum E2 in the three groups were (180. 6±62. 3), (130. 5±39.9) pmol/L and (110. 5±68. 5) pmol/L, respectively. Bone mass was decreased with the decreased serum E2 level(F=3. 059,P<0. 05). Conclusions BMD is declined with ageing, and the level of serum E2 may affect the occurrence of osteoporosis in elderly men.

Objective To develop an advanced two-cuff technique orthotopic liver transplantation model in rats. Methods On the basis of the previous two-cuff-technique, some hypercoagulable blood was released from the recipient's PV and IVC before the cuffed donor PV and IVC were inserted into the lumen of the recipient's PV and IVC. The anastomosis of recipient's SVC was between the donor's diaphragm venaccaval hiatus and recipient's SVC. Results Every anastomosis was effective. The operative successful rate and one-week survival rate were 96.7?% and 90.0?% respectively. Conclusion This model could be used as an effective measure for the study of liver transplantation in rats.

OBJECTIVE: To study the curative efficacy of Shuxuetong injection for vascular dementia(VD).METHODS: A total of 60 patients with VD were randomized to receive Shuxuetong injection(6 mL) added into normal saline(250 mL)q.d by drip infusion for 21 days(treatment group) or citicoline injection(250 mg) added into 250 mL normal saline q.d by drip infusion for 21 days(control group).In addition,both groups received basic treatment and nursing care,and the antihypertensive,hypoglycemic and hypolipidemic therapies were adopted based on the patients' severity of disease and complications.The efficacy was evaluated before and after treatment by using mini-mental state examination(MMSE),activities of daily living(ADL),transcranial Doppler(TCD) and P300 test.RESULTS: Compared to control group,the treatment group had significantly higher MMSE score and ADL score(P

Objective To study the association between vitamin D status and bone mineral density(BMD) in aged people.Methods A total of 118 patients admitted in cadre department aged (77.7±11.2) years were selected from July 2010 to May 2011.The BMD at lumbar spine (L1-4) and femoral neck was measured by dual energy X-ray absorptiometry (DEXA).According to BMD,patients were divided into two groups:osteoporosis and non-osteoporosis group.The data of serum 25-hydroxy vitamin D3 [25(OH)D3]concentration,height,weight and age of patients were collected at the same time.Results The age and body mass index (BMI) in osteoporosis group were (81.6 ±5.6) years and (22.5 ± 4.0 ) kg/m2,while (79.4 ± 6.9 ) years and (24.1± 4.2 ) kg/m2 in nonosteoporosis group (t=1.80 and -2.01,P＞0.05).The concentrations of serum 25(OH)D3 in two groups were(21.6± 10.3)nmol/L and (32.0± 13.8) nmol/L,respectively(t=-4.20,P＜0.01).And there were 95.3 ％ (41/43 )and 81.3 ％ (61/75) of patients whose serum 25 (OH)D3 level were ≤50nmol/L in osteoporosis and non- osteoporosis group,respectively(x2 =4.58,P＜0.05).Furthermore,the 25 (OH) D3 level was positively correlated with BMD at femoral neck(r=0.22,P＜0.05),but not correlated with BMD at L1-4 ( r=0.18,P＞0.05).Conclusions Vitamin D status is correlated with BMD at femoral neck in aged people.

ObjectiveThe purpose of the present study was to discuss the method to avoid spillage by means of adding chemotherapeutic drugs into sealed transfusion bottles.Methods90 penicillin sealed bottles of the same batch number were randomly divided into the test group and the control group,each with 45 bottles.Standard method according to Basic Nursing was used to add chemotherapeutic drugs into transfusion bottles in the control group.Drugs were added into transfusion bottles under negative pressure in the test group.The operating time was measured,the spillage volume of puncture site was calculated and microbial detection of syringe was observed in the two groups.ResultsThere was a significant difference in the spillage volume of puncture site between the two groups (P<0.01),but no difference was seen in the operating time and microbial detection of syringe (P>0.05),ConclusionsThe spillage volume of puncture site was reduced significantly by means of adding chemotherapeutic drugs into transfusion bottles under negative pressure.This can decrease chemotherapeutic professional exposing risks and drug wastage.

The paper described the practice of contingency nursing management for ICU wards in case of power breakdown in the hospital The SICU wards have developed their power failure contingency plan in which the nurses on duty are divided into Group A,B and C according to their positions and qualifications.Respectively,they take charge of patient safety,coordination and command,and medical aid.We also established the procedure of emergency response for these duties.Training and examination familiarize the medical personnel with the procedure and their duties.Four practices proved that the implementation of the plan reduces risks exposure and improves the satisfaction.With the contingency plan,the nurses in ICU can now take care of critical incidents and avoid nursing risks in ICU efficiently.

The medical service payment system reform is one of core issues in medical and health system reform.The payment system can directly affect behavior of stakeholders and indirectly affect medical expense,quality of care,efficiency of medical services and efficiency of medical insurance funds.Through behavior analysis of stakeholders in fee for service,DRGs-PPS,global budget,DRGs-PPS is the best payment system that can control the irrational increase of medical expenses and ensure the level of health service and meets interest of stakeholders among three payment systems.In DRGs-PPS,stakeholders continuously make progress in filling in the front sheet of medical records,clinical pathway,construction of information system,cost calculation and restructure costs.

Objective To investigate imaging and clinical findings of severe adenoviral pneumonia in children.Methods The imaging and clinical findings of severe adenoviral pneumonia in three children were retrospectively analyzed. All cases underwent radiography and CT examinations of chest. Results Two patiens showed fairly homogeneous opacity with air bronchograms in segmental or lobar distribution in left lung,in company with volume decreased in one case. There was interstitial change in 1 case,pleural effusion in one case and heart shadow enlarged in 3 cases. All of patients had cough and fever (range,38.4℃～40.3℃). Physical examinations showed decreased breathing sound and crackles in all cases. Laboratory findings included abnormally elevated aspartate aminotransferase,lactic dehydrogenase, hydroxybutyric dehydrogenase and creatine kinase and positive adenovirus examination in all cases. Conclusion Lobar pneumonia in company with liver and heart dysfunction should be suspected as the severe adenoviral pneumonia in children.