This study was aimed to analyze the volatile oil of Mentha crispata Schrad. ex Willd. in order to provide evidence for its chemotype and guidance for its production application. The chemical analysis was detected by headspace GC-MS. The results showed that 64 chemical compounds were detected. It was concluded that the volatile oil of M. crispata Schrad. ex Willd. mainly contained eucalyptol (35.58%), limonene (16.92%) and pinene (15.33%). It was concluded that the analysis on composition characteristics and main compounds of M. crispata Schrad. ex Willd. can provide evidences in its production application and chemotype.

To study the effects of intraperitoneal chemotherapy (IPC) plus Shenmai Injection (SMI) in treating advanced colorectal cancer following radical resections.

Objective To identify the species classification of an ornamental Planorbidae from a flower market in Shanghai and analyze its potential distribution in China. Methods In August 2013,six freshwater snail specimens were collected from the Wanshang flower market. The species was identified by morphology and molecular biology. An ecological niche model was constructed based on the native geographic presence occurrence data,and projected onto the whole of China to predict the poten?tial distribution. Results Their shell external morphology suggested that the specimens belonged to Planorbella trivolvis(Say 1817)of Planorbidae,which is native in North America. The sequence data of a fragment of the mitochondrial cytochrome c oxi?dase subunit I(COI)confirmed its identification. A total of 2 294 georeferenced occurrence points in North America were car?ried out from the Global Biodiversity Information Facility databases and 614 records with coordinates were used to produce a North American native niche model by a maximum entropy method(Maxent). The projection on China results suggested high probabilities of occurrence mostly in Henan Province and its borderland with nearby provinces. Conclusions P. trivolvis is sim?ilarly with Biomphalaria species from shell morphology. It is the first records of the species in China,and the field dispersal is not clear.

Objective To evaluate the effects of different concentrations of parecoxib sodium on the rat sperm motility,capacitation and acrosome reaction in vitro.Methods The sperm samples from Sprague-Dawley rat epididymis were collected by Klinefelter diffusion method and randomly divided into 4 groups (n =18 each) using a random number table:control group (group C),and parecoxib sodium 100,500,1 000 μmol/L groups (P1-3 groups).Parecoxib sodium with the final concentrations of 100,500 and 1 000 μmol/L was added to the culture medium.The samples were then incubated for 5 h in an airtight container filled with 5 ％ CO2 at 37 ℃.Then sperm motility was examined in vitro at 37 ℃ and analyzed by the computer-assisted sperm analysis,including the sperm motility ((a + b)％),average path velocity (VAP),straight line velocity (VSL),curvilinear velocity (VCL) and amplitude of lateral head displacement (ALH).The capacitation effect was assessed by using the chlortetracycline staining and phase-contract microscopy.The acrosome reaction was evaluated by coomassie brilliant blue staining.Results The VAP,VSL,VCL and capacitation ability of the sperm were gradually decreased in C and P1-3 groups (P ＜ 0.05).Compared with group C,(a + b)％ in P2,3 groups and ALH in P2 group were significantly decreased (P ＜ 0.05).There was no significant difference in the acrosome reaction between groups (P ＞ 0.05).Conclusion Parecoxib sodium has significant inhibitory effects on the rat sperm motility and capacitation in a dose-dependent manner,while has no effect on the acrosome reaction in vitro.

Epidermal growth factor receptor tyrosine kinase inhibitor (EGFR-TKI) targeted treatment has been a standard therapy for advanced non-small cell lung cancer (NSCLC), but it is not tolerated well by all patients. In China, some studies have reported that traditional Chinese medicinal herbs (TCMHs) may increase efficacy and reduce toxicity when combined with EGFR-TKI, but outside of China few studies of this kind have been attempted.

Objective: To prove the clinical therapeutic effect of acute and chronic bronchial asthma with Chuankezhi(CKZ) Injection. Methods: 40 cases of bronchial asthma, including inpatients and outpatients were selected, divided into the attack period group (20) and the relief period group (20). The former was intramuscularly injected CKZ injection 4mL, twice a day, 14 days being a course of treatment. The latter was intramuscularly injected CKZ injection 4mL, twice a day for 15～30 days and 2mL, every other day for 31～90 days; 90 days being a course of treatment. The symptoms and signs, such as asthma, cough, expectroation, pulmonary wheeze were observed. and pulmonary function, absolute eosinophilic granulocyte count and immunogobulin E were measured before and after treatment. Result: By the use of CKZ injection to treat bronchial asthma, the total effective rate amounted to 85% and obvious difference before and after treatment in the two groups ( P

Laser-induced breakdown spectroscopy ( LIBS) was used to detect the compositions of soil in the air, and the quantitative analysis model with genetic algorithm-partial least squares ( GA-PLS ) was established. A total of fifty-eight soil samples were split into calibration, monitoring and prediction sets. Eleven soil compositions including Mn, Cr, Cu, Pb, Ba, Al2 O3 , CaO, Fe2 O3 , MgO, Na2 O, and K2 O were quantitatively analyzed. The results demonstrated that, as a pretreatment method for optimizing the selection of spectral lines, GA could be effectively used to reduce the number of spectral lines for use in building PLS model, and hence simplify the quantitative analysis model. More importantly, for most of the soil compositions, GA-PLS could significantly improve the prediction ability compared with the conventional PLS model. Take Mn as an example, the root-mean-square error of prediction ( RMSEP ) was decreased from 0. 0215% to 0 . 0167%, and the mean percent prediction error ( MPE ) was decreased from 8 . 10% to 5 . 20%. The research provides an approach for further improving the accuracy of LIBS quantitative analysis in soil.

OBJECTIVETo study effects of different harvest times and processing methods on the quality of Gardeniae Fructus.

METHODThe content of asminoidin and the similarity of the fingerprint of Gardeniae Fructus were determined and applied for assessment of the quality.

RESULTGardeniae Fructus harvested in October with yellow-green appearance was the better time point for harvest. Drying in the sun and boiling in the water is the better processing method for Gardeniae Fructus.

CONCLUSIONDifferent harvest times and processing methods can affect the quality of Gardeniae Fructus.

Drugs, Chinese Herbal ; chemistry ; standards ; Fruit ; chemistry ; growth & development ; Gardenia ; chemistry ; growth & development ; Quality Control

Objective To explore the efficacy ofTiaogan-Bushen-Xiaoji recipe (TGBSXJ Recipe) on overall survival and progression free survival (PFS) in patients with advanced breast cancer.Methods A total of 105 patients with advanced breast cancer, who received the first-line treatment, were divided into two groups, 56 cases in the control group and 49 in the experimental group. The control group received standard therapy according to guidelines, including chemotherapy, targeted therapy, endocrine therapy and treatment of bisphosphonates.The experimental group received the treatment of TGBSXJ Recipe besides the standard therapy. The overall survival (OS), progression-free survival (PFS) and Karnofsky (KPS) of the patients in two groups were observed and compared. The treatment ended with the sighs of the observation ending, the second progress for the disease or death.Results The overall survival of the experimental group was significantly higher than that of the control group [OS: 58.2(50.7-65.8)/monthsvs. 43.8(30.6-51.6)/months,P=0.040]. The PFS of the experimental group was significantly higher than the control group [PFS: 30.7(23.8-37.7)/monthsvs. 15.2(11.3-19.1)/months,P=0.001]. The KPS of the experimental group was significantly higher than the control group (88.6 ± 10.0vs. 80.5 ± 19.0,t=2.654). The PFS of the triple negative breast cancer (TNBC) in the experimental group was significantly higher than control group [(25.1(12.1-38.0)/monthsvs. 9.9(4.7-15.0)/months,P=0.038].Conclusions The TGBSXJ recipe could extend the OS and PFS and improve the life quality of the patients with advanced breast cancer. In this study, no severe adverse effects had been found in the experimental group.

Objective To investigate the effects of 20％ fluor-hydroxyapatite(FHA) on proliferation and osteogenic differentiation of human MG63 osteosarcoma cells.Methods FHA was prepared by chemical precipitation method,and its structure and surface features were tested by scanning microscope,Xray diffraction(XRD) and Fourier transform infrared spectroscopy.MG63 cells were cultured and divided into FHA,hydroxyapatite(HA) and control groups(n=3).The proliferation of the cells was evaluated using methylthiazol tetrazolium(MTT) assay.ALP activity of the cells was assessed.Osteogenic differentiation was evaluated based on the reverse transcription PCR(RT-PCR) of differentiation-related genes,namely,collagen type Ⅰ(Col Ⅰ),alkaline phosphatase(ALP),osteocalcin(OCN) and core binding factor α1(Cbfα1).The data were analyzed statistically by one-way analysis of variance using SPSS 13.0 software.Results XRD test showed that the main crystalline phase of FHA was similar to that of HA.Absorptance value of cells exposed to FHA(1.87±0.06) measured by MTT was higher than that of the control(1.25±0.02) on the third day(P＜ 0.05),and there was no statistically significant difference between the cells exposed to FHA and HA(1.84± 0.03)(P＞0.05).ALP activity of the cells exposed to FHA (4.62±0.09) was higher than that of the control (1.92 ± 0.05)(P＜0.05).RT-PCR tests showed that compared with the control,FHA up-regulated the expression of Col Ⅰ,ALP and OCN mRNA,down-regulated the expression of Cbfα1 mRNA.Conclusions FHA enhances the proliferation and osteogenic differentiation-related gene expression,and has good biocompatibility.