Purpose To investigate the correlation of CT features with peripheral blood eosinophil count and proportion in eosinophilic gastroenteritis (EG).Materials and Methods Seventeen cases of clinically confirmed EG were retrospectively analyzed.The lesions were observed based on CT findings,and the count and proportion of peripheral blood eosinophil were calculated.Results Among 17 cases of EG patients,2 cases had one lesion,the peripheral blood eosinophil count and proportion of which were (0.28±0.22)× 109/L and (4.85 ± 2.19)％,respectively;5 cases had two lesions,the eosinophil count and proportion of which were (3.19± 1.82)× 109/L,(37.04± 11.36)％,respectively;6 cases had three lesions,the eosinophil count and proportion of which were (3.93 ±2.37)× 109/L and (45.22±22.99)％,respectively;3 cases had four lesions,the eosinophil count and proportion of which were (6.48±0.34)× 109/L and (75.83± 9.33)％,respectively;1 case had seven lesions,the eosinophil count and proportion of which were 8.87× 109/L and 75.60％,respectively.Conclusion There is a certain correlation of the peripheral blood eosinophil count and proportion with EG lesions.When the count and proportion of peripheral blood eosinophil increase significantly,the range of lesions may become more extensive,which suggests the scope of endoscopy or imaging examinations should be expanded in clinical work.

The factor analysis method applied in imaging mass spectrometry data analysis was studied. The imaging mass spectrometric data were obtained by air flow-assisted ionization imaging mass spectrometry method. The sample contained some symbols which were drawn on slides using three different inks ( red, blue, black) . The imaging data analyzed by factor analysis method were divided into the background, black, blue and red factor. The results showed that the scores of m/z=443. 2, 478. 4, 322. 2(344. 2) in red, blue, black factor respectively were much larger than others. Therefore, they were markers of three inks. The results accorded with actual condition well and proved that the application of factor analysis in imaging mass spectrometric data analysis was feasible. The data analysis results of factor analysis and principal component analysis were compared. The results showed that the target sample markers could be extracted by factor analysis simply and quantitatively. It was of great potential in biomarker extraction, diseases diagnose and pharmacological analysis.

Purpose To explore the clinical features and multi-slice spiral CT (MSCT) imaging characteristics of nodule-type and mass-type parotid eosinophilic lymphogranuloma,in order to improve the understanding of the disease.Materials and Methods The clinical and imaging data of 6 patients with nodule-type or mass-type parotid eosinophilic lymphogranuloma confirmed by surgery pathology in Chongqing Jiulongpo Chinese Medicine Hospital from June 2007 to March 2016 (experimental group) were retrospectively analyzed.Another 32 synchronous patients with Warthin tumor confirmed by surgery pathology were enrolled as the control group.MSCT conventional and enhanced scanning were performed on all these patients,and the clinical features and MSCT characteristics were compared between the two groups.Results Compared with patients with Warthin tumor,the value and percentage of peripheral eosinophil and serum IgE level increased in all the 6 patients (age ＜ 50) with eosinophilic lymphogranuloma,and the difference was statistically significant (P＜0.01).Compared with Warthin tumor,eosinophilic lymphogranuloma was often accompanied by ipsilateral parotid enlargement,neck deep fascia thickening and edge blurring,lesions reducing,and neck lymph node enlargement.The enhanced scanning of eosinophilic lymphogranuloma showed delayed enhancement,and the enhancement in venous phase and delayed-phase was significant;while Warthin tumor showed significant enhancement in arterial phase and contrast clearing features in delayed-phase.Conclusion The MSCT findings of eosinophilic lymphogranuloma characterized by nodules or masses shows features of inflammatory lesions,in which mass density is relatively uniform and presents delayed enhancement.Combining clinical manifestation with laboratory tests will contribute to distinguish eosinophilic lymphogranuloma and Warthin tumor.

Objective To analyze the deep venous thrombosis(DVT)after plasma infusion in a patient with congenital dysfibrinogene-mia(CD),and explore the relationship between the CD and DVT.Methods The clinical data were collected and the pedigree was investigated(3 subjects of 2 generations in total).The relevant indexes of coagulation factors of the patient and her family members were detected.The genomic DNA of peripheral blood was extracted for PCR amplification.All the exons,flanking sequences,5'and 3'untranslated regions of FGA,FGB and FGG genes of fibrinogen(Fg)of the patient were analyzed by direct sequencing.The corre-sponding mutation site was subjected to sequence in the other members of this family.The PyMol software was used to construct the pro-tein model before and after gene mutation.Results The patient was admitted to hospital for hysteromyomectomy.DVT appeared in 3 days after surgery.The prothrombin time(PT),thrombin time(TT),Fg activity(Fg∶C)and Fg antigen(Fg∶Ag)of the patient was 14.9 s,33.3 s,0.94 g/L and 2.10 g/L,respectively.The above four indicators in her mother were 14.7 s,32.8 s,0.97 g/L and 2.35 g/L,respectively.Gene sequencing revealed that both the patient and her mother had a heterozygous missense mutation c.2185G＞A(p.Glu729Lys)in exon 6 of the FGA gene.The protein model analysis demonstrated that p.Glu729Lys mutation changed the amino acid side chain and reduced the number of hydrogen bonds originally formed with Arg854.Conclusion A heterozygous missense mutation c.2185G＞A(NM_000508)in exon 6 of the FGA gene should be responsible for the low fibrinogen level in this pedigree,which might be the main reason for DVT after plasma infusion in this patient.