Objective To analyze the correlation of C -reactive protein level and acute cerebral hemorrhage prognosis,provide the evidence for determining the condition and prognosis.Methods 163 patients with acute spon-taneous intracerebral hemorrhage met selection criteria in Yantai Economic and Technology Development Area Hospi-tal were selected as the study objects,the CRP were detected by immunoturbidimetry in the 1 day,3 days,7 days, 14 days after admission,the NIHSS were calculated,48 cases of healthy people were selected as the control group,the CRP between hemorrhage group and the healthy control group,patients with different brain hemorrhage,patients with different neurological deficit were compared.Results (1 )The C -reactive protein of cerebral hemorrhage group when admission were (8.92 ±1.0)mg/L,the control group were (4.25 ±0.23)mg/L,the C -reactive protein of cer-ebral hemorrhage group were significantly higher than that of the control group,the difference was statistically signifi-cant,t =-3.683,P <0.01.(2)The mean serum CRP after admission admission 1,3,7,14d were (9.34 ±1.65) mg/L,(9.56 ±1.58)mg/L,(6.58 ±1.17)mg/L,(4.98 ±1.26)mg/L,the CRP was a large rise in the course of the disease 1d,3d peak,F =7.487,P <0.05,the CRP had difference between large amount of brain hemorrhage,moder-ate amount of brain hemorrhage,and small amount of brain hemorrhage,a large amount of patients with cerebral hem-orrhage CRP levels were higher than a moderate amount of patients with cerebral hemorrhage and a small amount of patients with cerebral hemorrhage 1,3 and 7d,during which the difference was statistically significant(F =3.31, 4.62,4.21,P <0.01 or P <0.05).(3)The CRP peak levels of different degrees of neurological deficits in patients with acute cerebral hemorrhage decreased in 3d,7d,14d,the CRP of 3d were higher than that in 1d,the CRP of 7,14d were lower than that in 1d,CRP levels between different neurological deficits degree of acute cerebral hemorrhage were also significantly different (F =4.29,5.32,3.62,3.21,P <0.05 or P <0.01),the CRP levels of Severe neurological deficit in 1d,3d,7d,14d were significantly higher in mild and moderate neurological deficit (t =6.567,4.241, 4.010,3.511 and 4.965,3.031,3.104,3.009,P <0.05 or P <0.01 ).Conclusion The serum CRP levels of patients with acute cerebral hemorrhage significantly increased,the serum CRP level shows dynamic changes in the first three days of the onset of peak,the CRP levels significantly correlated with the prognosis of cerebral hemorrhage, the more severe neurological deficit,the greater the amount of cerebral hemorrhage,higher serum CRP levels.

Objective To develop a radiology algorithm and test its the accuracy in distinguish pacing in the septum from the other parts. Methods One hundred patients were implanted with double-chamber pacemakers. Sites of the leads were verified by two-dimensional echocardiography, and the patients were divided into 4 groups according to the echocar?diography:septal right ventricular outflow tract group(RVOT), RVOT anterior free wall group, mid septum group, and anteri?or septum group (near to the anterior free wall ). An algorithm was developed according to radiological characteristics in the 45° left anterior oblique (LAO45° ) view and the 30° right anterior oblique (RAO30° ) view. Then its sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) were tested . Results The algorithm has high sensitivi?ty and specificity, which were 90%and 97%respectively. The positive predictive value and negative predictive value were 90% and 97% respectively. Conclusion The radiology algorithm we developed have a high sensitivity and specificity in identifying the site of the leads.

10.3969/j.issn.2095-4344.2013.23.014

BACKGROUND:In recent years, embryonic hepatic stem cel s have attracted more attention, but there are few reports on the potential of embryonic hepatic stem cel s to differentiate into cardiomyocyte-like cel s as wel as the related differentiation conditions. OBJECTIVE:To investigate the moderate condition to induce mice embryonic hepatic stem cel s to differentiate into cardiomyocyte-like cel s in vitro with chemical reagents. METHODS:Dimethylsulfoxide in combination with 5-azacytidine with different concentrations and time were used to induce the embryonic hepatic stem cel s of 13.5 days mice and to observe the differentiation effect. RESULTS AND CONCLUSION:Under in vitro conditions, 0.8%dimethylsulfoxide+5μmol/L 5-azacytidine could induce the mouse embryonic hepatic stem cel s to express the specific markers of myocardial cel s, while increasing the concentration of the inducer and extending the induction time could not improve the induction efficacy.

BACKGROUND: Fetal liver cells can have stronger abilities to proliferation and differentiation and lower immunogenicity compared to bone marrow stem cells. However, there are few studies on direct isolation and culture of embryonic hepatic stem cells (EHSCs). OBJECTIVE: To isolate and cultivate EHSCs in vitro and to identify their biological features. DESIGN, TIME AND SETTING: The cytology in vitro controlled study was performed at the Chongqing Key Laboratory of Neurology from March to June 2008. MATERIALS: A total of 9 SPF Kunming fetal mice aged 13.5 days were obtained from Animal Experimental Center of Chongqing Medical University. METHODS: Collagenase + EDTA digestion and differential adherence method were used to isolate EHSCs, which were then incubated at 2?108 /L. Cells were digested and passaged when 80%-90% cells were confluent. Using streptavidin-biotin-peroxidase complex technique, adhered cells following 5 days of incubation were labeled with various EHSC surface marker. MAIN OUTCOME MEASURES: Morphology, passage, amplification and surface marker surface of EHSCs were measured. RESULTS: The isolated EHSCs adhered to the culture plastic and presented pykno-round cells and distinct borderline 24 hours after cultivation in vitro. Cells grew spindle-shaped 3 days. After 7 days they grew like epithelium. Cell amplified speed following passage did not have significant changes. Cells still presented epithelium-like shape at the passage 5. The adhered cells at day 5 following primary incubation were positively for human stem cell factor receptor and alpha fetoprotein, and negatively for albumin and cytokeratin 19. CONCLUSION: EHSCs were positively for human stem cell factor receptor and alpha fetoprotein, and negatively for albumin and cytokeratin 19 in early primary culture. This indicated that the cultivated cells are proved to be primordial progenitor cells and still in undifferentiated early phase.

Objective The expression of anti-inflammatory cytokine interleukin(IL)-10 in protrusion and extrusion tissue of intervertebral disc and its correlation with clinical features.Methods The specimens were from 30 cases of disc herniation in single level.The patients were enquired about the medical histories and evaluated by JOA index preoperation before operation,which were all completed by one trained doctor.The contents of IL-10 in the inter=43vertebral disc were detected by enzyme-linked immunosorbent assay after operation.Results The level of IL-10 had no statistical significance between protrusion and extrusion tissue of intervertebral disc (t =0.032,P =0.725).Patient's JOA index has negative correlation with IL-10(r =-0.377,P =0.040),and positive correlation with Patient's history (r =0.449,P =0.013).Conclusion Protrusion and extrusion tissue of intervertebral disc have abilities of excreting anti-inflammatory cytokine IL-10.The level of cytokine has correlation with patient's clinical features.

BACKGROUND: Hundreds of mouse embryonic stem cell lines are established presently. Most of these cell lines are collected from 129, C57BL/6J and BALB/C mice. Success rate of creating embryonic stem cell lines with Kunming mice is very low. OBJECTIVE: To explore the method of isolation and culture of murine embryonic stem cells of kunming species to increase the efficiency of establishment of embryonic stem cell lines from Kunming species mouse. DESIGN, TIME AND SETTING: The cell experiment was performed at the Chongqing Key Laboratory of Neurology from April 2006 to June 2007. MATERIALS: Blastocysts of 3.5 days and 4 days from Kunming species mouse were collected. METHODS: The blastocysts of 3.5 days and 4 days from Kunming species mouse were cultured on the fibroblast cell feeder layers for 4-5 days, and the cells from inner cell mass were isolated and subsequently cultured in vitro in 2.5 g/L trypase-0.4 g/L ethylenediamine tetraacetic acid (EDTA) and 1 g/L trypase-0.2 g/L EDTA at room temperature for 2-4 minutes. Embryonic stem cell colony was mechanically straggled. MAIN OUTCOME MEASURES: Colony growth was observed and determined by alkaline phosphatase staining, OCT-4 staining and karyotype analysis. RESULTS: Adherence rate, inner cell mass frequency and cloning efficiency of 4 day post coitum (dpc) embryo were higher than those of 3.5 dpc embryo (P

Objective To explore the effect of embryonic stem cells(ESCs) differentiating into cardiomyocytes(CM) by cardiomyocytes.Methods The blastocytes of 3.5 d from Kunming mouse,cultured on mouse embryonic fibroblaste cell feeder layers for 4～5 d,the mouse ESCs were isolated and subsequently cultured.Day 2～3 embryoid bodies(EBs) were derived from ESCs of passage 3 to 5 and then incubated with neonatal cardiomyocyte(CM) to induce into cardiomyocytes and the expression of cardiac specific cardiac troponin-T(TnT) and ?-actin were detected by immunofluoresence.Results The rhythmic beating embryoid bodies were observed on day 3 and reached 93% of rhythmic beating embryoid bodies on 12 day in group 2.All the beating cardiomyocytes derived from ESCs expressed cardiac-specific proteins for TnT and ?-actin,and the group induced by direct cell contact acquired the highest differentiating ratio as 56.5%,and was the highest differentiating ratio compared with other groups(P

Objective To investigate the changes in metabolic indexes and anesthesia administration during perioperation in patients undergoing orthotopic liver transplantation. Methods Eleven patients who underwent orthotopic liver transplantation were selected. Their pH, BE, HCO 3 -, K +, Ca 2+, blood glucose, PT, APTT and FIB were assessed at 60 min of pretransplantation, 20 min and 40 min of liver transplantation, 20 min and 40 min of new liver reperfusion. Results The pH, BE, HCO 3 -, K +, Ca 2+ during 20 min and 40 min of liver transplantation decreased more significantly than those during pretransplantation 60 min, PT and APTT extended markedly, the level of blood glucose did not change markedly; pH, BE, HCO 3-, K +, Ca 2+ and the level of blood glucose during new liver reperfusion 20 min and 40 min increased significantly compared to those during liver transplantation 20 min and 40 min; PT, APTT and FIB did not change markedly. Conclusion Metabolic indexes during liver transplantation change more markedly than those during pretransplantation or new liver reperfusion.

Invasion of glioma is a complex process with multiple steps and multiple factors,including the inhibition of tumor cell adhesion,the degradation of extracellular matrix,the promotion of tumor cells migration and angiogenesis.The abnormal activation of Wnt/β-catenin signaling pathway is closely related to the invasion of glioma.The study of Wnt/β-catenin pathway affecting invasion mechanism of glioma will provide new ideas and targets for the treatment of glioma.