OBJECTIVE: To establish the preparation process and TLC for Rutong Capsules. METHODS: The preparation process was optimized by orthogonal experiment design. The components in the preparation were identified qualitatively by TLC, and the quality standard of the preparation was established. RESULTS: Radix Angelicae Sinensis, Fructus Aurantii, Radix Bupleuri were able to be identified by TLC, and the physical and chemical characteristics of Poria and Rhizoma Atractylodes macrocephala were able to be identified. CONCLUSION: The method adopted in the study is simple, accurate and reproducible, and it is applicable for the preparation and TLC of Rutong Capsules.

OBJECTIVE:To optimize the technologic conditions for separation and purification process of Astragaloside Ⅳ using macroreticular resins.METHODS:The optimal extraction process and purification technology using macroreticular resins were investigated by orthogonal experiment design with extract yield and content of Astragaloside Ⅳ as evaluation indexes,and the influencing factors were also investigated.RESULTS:The optimal conditions for extraction were as follows:sixfold amount of water was added to extract Astragaloside Ⅳ for 3 times with 1 h each time.The isolation and purification of Astragaloside Ⅳ were influenced by relative density,elution dosage and velocity.CONCLUSION:The established optimal technologic conditions for the extraction,separation and purification of Astragaloside Ⅳ using macroreticular resins proved to be feasible.

In recent years, great progress has been made in the pathogenesis of psoriasis, which has provided multiple new targets for the treatment of psoriasis, and ushered the treatment, especially topical treatment, of psoriasis to a new era. Some new targeted topical drugs with action mechanisms different from those of traditional drugs have been applied in clinical practice, which are mainly small -molecule inhibitors targeting cell signaling pathways or enzymes involved in the pathogenesis of psoriasis, such as the Janus kinase/signal transducer and activator of transcription(JAK/STAT) pathway, phosphodiesterase type 4 (PDE4), mitogen-activated/extracellular-signal-regulated kinase kinase (MEK), vascular endothelial growth factor receptor (VEGFR), tropomyosin receptor kinase A (TrKA), retinoic acid receptor-related orphan receptor γ (RORγ), and so on. At present, many phaseⅠ and Ⅱ clinical trials are conducted for these drugs. Some completed trials have shown that they have good effectiveness, safety and tolerance, providing more options for the topical treatment of psoriasis.

Objective To investigate the correlation between the anti-nuclear antibody(ANA)pattern and the specific ANA spectrum.Methods 974 cases of detected ANA results in our hospital were analyzed retrospectively.The ANA pattern was detec-ted by the indirect immunofluorescence(IIF)and the specific ANA spectrum was tested by the line immunoassay(LIA).199 cases of both simultaneous positive results by IIF and LIA were analyzed and the correlation between ANA patterns and specific ANA spectrum was analyzed.Results Among 974 cases of specimen,249 cases(25.6%)were positive by IIF and 237 cases(24.3%) were positive by LIA,the difference in the positive rate between IIF and LIA had no statistical significance(P >0.05 ).287 cases (29.5%)were positive by single IIF or LIA or both IIF and LIA,which was higher than the positive rate by single IIF or LIA (P <0.05).Among 249 cases of positive by IIF,119 cases(79.9%)were positive by LIA,among 725 cases of negative by IIF,687 (94.8%)cases were negative by LIA.The anti-Ro-52 antibody was frequently detected in the nuclear speckled pattern,and the an-ti-M2 antibody was detected more frequently in the cytoplasmic speckled pattern,anti-dsDNA antibody and anti-nucleosome anti-body were more frequently detected in the nuclear homogeneous pattern,anti-centromere antibody was more frequently detected in the centromere pattern and anti-PM-Scl antibody was more frequently detected in the nucleolus pattern.Conclusion The ANA de-tection has better correlation between IIF and LIA,but also has certain difference.Their combined detection can reduce the missed detection rate and has the important significance to the diagnosis,disease condition monitoring and prognosis judgment of autoim-mune diseases.

Objective To analyze the application rules of Jiaotong Xinshentreating principle in modern clinic based on TCM inheritance auxiliary platform.MethodsJiaotong Xinshen treating principle related prescriptions in CNKI and Wanfang databases were analyzed.“Jiaotong Xinshen”and “Xinshen Bujiao” were set as key words to retrieve relevant prescriptions in the databases. TCM inheritance auxiliary platform (V2.5) software was used to establish database. Data mining methods, such as frequency statistics and association rules were used to analyze new prescriptions aboutJiaotong Xinshen treating principle guided medication rules.ResultsTotally186 prescriptions were collected and analyzed. Medicine frequency and association rules among medicines in the prescriptions were confirmed. 12 medical combinations and 6 new prescriptions were mined.ConclusionThe data management platform and data mining methods can be applied in discovering the prescription patterns and medications rules ofJiaotong Xinshen treating principle in clinical application.

OBJECTIVE To assess the adherence ability of Candida albicans to two denture base resins with different surface roughness.METHODS In adhesion assays,two denture base resins were made into standard test samples with different surface roughness.Suspension of C.albicans(ATCC 10261) strain was used to adhere on the test samples,and cultured to observe the adhesive effect of a mixed salivary pellicle with Candida to these surfaces.RESULTS In glass surface group,the amount of C.albicans adhering to heat-curing denture base resins was 15.33?2.80,and the amount of C.albicans adhering to Valplast flexible denture resin was 13.67?2.94;In gypsum surface group,the amount of C.albicans adhering to heat-curing denture base resins was 22.67?4.84,and the amount of C.albicans adhering to Valplast flexible denture resin was 21.17?3.31.Significantly greater adhesion of C.albicans to rough surfaces rather than smooth surfaces of the same material was found,at the same time C.albicans had different adhesive ability on different denture base resins(P

[Objective]To investigate the reasons of misdiagnosis of colon cancer complicated with appendicitis and to sum up the experience of diagnosis. [Methods] Between May 1995 and September 2005, 1060 cases with appendicitis were undergone appendectomy. However, of them, 9 cases complicated with colon cancer had confirmative diagnosis during the operation or after, performed successfully suitable operations. [Results] All of 9 cases were misdiagnosed as appendicitis and undertook appendectomy for similar clinical traits. According to the signs of exploration in the appendectomy and the analysis of patient history, clinic characteristic and some radiologic features, we acquired confirmative signs of carcinoma and put up reasonable surgical intervention. [Conclusion] There are some imperative measures to decrease misdiagnosis by analyzing clinical manifestations comprehensively, observation dynamically, exploration in the appendectomy, and effective examination. Among them, the key to reduce misdiagnosis rate is exploration during the period of appendectomy.

Objective To study the imaging diagnostic methods of fibrous dysplasia.Methods 31 patients with fibrous dysplasia proved pathologically were studied. In addition to CT scan in 11 cases and MRI in 6 cases,plain films were performed in all 31 cases. The compartive analysis was made between the features of plain film and CT.Results Of 31cases, 18 patients had monostotic fibrous dysplasia and 13 polyostotic lesions. Roentgenologically, they were divisible into 4 type: cystic type(18 lesions),diffuse type(30 lesions),sponge groud-flesh type(2 lesions) and moth-eaten type(2 lesions). CT features of lesions were classified into 2 type: cystic expanded type(38 lesions) and osteosclerotic type(7 lesions).Conclusion X-ray film should be used as the first choice in diagnosis of fibrous dysplasia. CT is hightly sensitive in displaying the craniofacial lesions.The lesions can be correctly diagnosed in most instances,if plain film combined with CT.

Objective To determine the clinical and serological characteristics of autoimmune hemolytic anemia (AIHA) in systemic lupus erythematosus (SLE) patients.Methods Seventeen patients were studied who had clinical features of AIHA among 222 hospitalized SLE patients in the Department of Rheumatology of Beijing Chaoyang Hospital were studied.Among the SLE patients without AIHA,34 patients were chosen by random sampling as controls.The patients' demographic data,clinical manifestations,laboratory test results and other examinations were analyzed retrospectively.Student's t test was used for the comparison of continuous variables,and chi-square test was used for the comparison of proportions.Results The prevalence of AIHA was 7.6％ in cohort of lupus patients.The positive rate of IgG anticardiolipin antibody in the AIHA group was significantly higher than that in the control group (71％ vs 15％,x2=15.366,P＜0.01 ).The occurrence of antiphospholipid antibody syndrome between the two groups had no statistical significant difference (x2=0.000,P=1.000).The frequencies of arthritis (12％,x2=4.554,P=0.033),malar rash (6％,x2=4.443,P=0.033),leukocytopenia (12％,x2=8.061,P=0.005) and lymphocytopenia (41％,x2=5.075,P=0.024) were lower in the AIHA group than those in the control group.Three patients in the AIHA group had alveolar hemorrhage in the course of SLE,while there was none in the control group (x2=3.586,P=0.058).Conclusion When lupus patients with AIHA are compared with those without AIHA,the positive rate of anticardiolipin antibody is higher,but the occurrence of antiphospholipid antibody syndrome does not increase in SLE patients with AIHA.And the frequencies of arthritis,malar rash,leukocytopenia and lymphocytopenia are lower in those patients with AIHA,however,patients with AIHA are prone to develop alveolar hemorrhage.

Objective To investigate the mechanism of repression of testosterone biosynthesis in rats exposed to dibutyl phthalate (DBP). Methods Immature male rats were randomly divided into 5 groups involving 4 experimental groups and one control group. Animals were administered orally either corn oil only (vehicle control) or DBP (0.25, 0.50, 1.00, 2.00 g/kg) for 30 days. After 30 days exposure, the half of animals were sacrificed with testis, epididymis and adrenal selected and weighed. The ratio of organ to body weight were calculated and histopathological examination were examined. Testosterone (T), luteinizing hormone (LH), 17?-estradiol (17?-E2) and corticosterone (CORT) were detected by radioimmunoassay. After 15 days, the rest of animals were sacrificed and above-mentioned indexes were measured. Results The ratio of testis and epididymis to body weight reduced significantly at doses of 0.50 g/kg DBP and above. The testicle atrophy was observed, germinal epithelium became thin and the amount of reproductive cells obviously decreased. All of those were not repaired in convalescence stage. In above 1.00 g/kg DBP groups, the level of T, LH and 17?-E2 decreased (P