Objective To explore the application of rapid rehabilitation concept in perioperative period of colorectal cancer.Methods A total of 110 patients with rectal cancer admitted in our hospital from June 2012 to July 2015 were enrolled in the research group(55 cases)and control group(55 cases).The observation group was treated with rapid rehabilitation method,and the control group was treated by traditional perioperative method.Observation of the two groups of patients was in the hands of the amount of bleeding,operation time,the first time the patient exhaust time,postoperative time to get out of bed and other indicators.We observed the two groups of patients with surgical incision infection,intestinal obstruction,pulmonary infection and other complications.Results The difference of the amount of bleeding and the length of the incision in the two groups was statistically significant(t=9.618,7.846,P<0.01).The first time of exhaustive time,the first defecation time,the time of eating,the time of getting out of bed and the postoperative hospital stay of research group were significantly lower than those of the control group(P<0.01).The incidence of complications such as incision infection,intestinal obstruction and pulmonary infection of the research group was lower than those in the control group,and the difference was statistically significant(χ2=4.767,P<0.05).Conclusion The application of rapid recovery concept in perioperative period of colorectal cancer can reduce the incidence of surgical complications.

Rheumatic diseases are characterized by immunological dysfunction.T-cell medicated immune response plays a very important role in the development and persistence of rheumatic disease.CTLA-4 is an important co-stimulatory molecules expressed on T cells.It has inhibitory effect on T cell-mediated immune response.Plenty of evidences have demonstrated abnormalities in CTLA-4 alleles and proteins in patients with rheumatic diseases.Therefore,CTLA-4 has become an target in treating rheumatic diseases.CTLA-4Ig has been developed as an fusion protein which is composed of extrA-cellular domain of human CTLA-4 and the Fc fragment of human IgG1.So far,only data about CTLA-4Ig in treating rheumatoid arthritis are available.These data showed that CTLA-4Ig could relieve the symptoms of rheumatoid arthritis and retard the development of the disease.It is safe and effective.In addition,effectiveness was demonstrated even in patients who failed to response to anti-TNF monoclonal antibodies.Therefore,CTLA-4Ig is an encouraging therapy for rheumatic diseases.However,because of very short history,long-term study is needed to fully understand its role in the treatment of rheumatoid arthritis.

The osteoclast is a hematopoietic cell derived from CFU GM, which is regulated by systemic hormones, soluble factors in the phase of differentiation, fusion and proliferation. The major singnaling pathways involved in osteoclastic bone resorption and formation in response to cytokine and hormones include cAMP and R PTK. The advances in our understanding of the osteoclast make it possible for us to comprehend the development and the treatment of the bone metabolic diseases.

Methamphetamine is a powerful stimulant of amphetamine type in the central nervous system (CNS),and recently it has become the major drugs of abuse.A lot of research results show that MA may induce dopaminergic neurotoxicity in the animal and human striatum.The mechanisms include effects on dopaminergic signaling and dopamine oxidation,glutamate induced excitotoxicity,oxidative stress and inflammatory cytokines,disruption of mitochondfia,apoptosis,activation of glial cells and hyperthermia.However the mechanisms by the MA-induced dopaminergic neurotoxicity are not completely understood.The paper reviewed recent progress of study on them to provide reference materials for related research.

Objective To explore the diagnostic value of anti cyclic citrullinated peptide (CCP) antibody (anti CCP) detected by ELISA in patients with rheumatoid arthritis (RA).Methods The synthesized CCP deduced from the known cDNA sequence was used as substrate in ELISA.The anti CCP was detected by ELISA in 294 RA,132 rheumatic diseases other than RA and 135 non rheumatic diseases.To compare the correlation among the anti CCP and APF (antiperinuclear factor),AKA (anti keratin antibody),RF (rheumatoid factor) and HLA DR4.Results One hundred and thirty seven (46 6%) RA sera,7 (5 3%) other rheumatic disease sera and 2 (1 8%) non rheumatic disease were found to be anti CCP positive by ELISA.The sensitivity of anti CCP was 46 6% with high specificity (96 9%) in RA.Anti CCP was associated with APF,AKA and HLA DR4 except RF.Conclusions Anti CCP presents a reasonable sensitivity (46 6%) with high specificity (96 6%) to RA and it is valuable to RA diagnosis.Anti CCP is associated with APF,AKA and HLA DR4 but not completely overlaps them.Anti CCP could be regarded as a new diagnostic marker in RA.

Objective To establish the method for extracting the cytokeratin filament aggregating protein (filaggrin) from human epidermis,to detect the anti filaggrin antibody (AFA) in rheumatoid arthritis (RA) and to determine the diagnostic value of AFA in RA.Methods Filaggrin was extracted from human epidermis and partially purified.AFA in 103 patients with RA and 140 controls was detected by Western blotting.Results Filaggrin extracted from the epidermis could be used as the antigen in AFA test by Western blotting.AFA test resulted in diagnosis of 35 9% of 103 RA samples,with a specificity of 93 7%.The result was significantly higher compared with patients′ and normal controls ( P

Objective To further understand the etiology,definition,clinical manifestation,prognosis and treatment of cutaneous leukocytoclastic vasculitis.Method Retrospective study of23patients with leuko-cytoclastic vasculitis followed up at Peking Union Medical College Hospital from1990to2001.Results Twenty patients were classified as hypersensitive vasculitis,one of whom was diagnosed as Wegeners granulo-matosis2.5years later,two were diagnosed as urticarial vasculitis,and one was systemic lupus erythematosus.Conclusion Cutaneous leukocytoclastic vasculitis,which is usually a benign syndrome,may be caused by in-fection or drugs.Its main clinical manifestaions are skin symptoms.

Objective To determine the diagnostic value of antifilaggrin antibodies(AFA) in rheumatoid arthritis(RA) and to compare the correlation among AFA,anti-perinuclear factor(APF) and anti-keratin antibodies(AKA). Methods Filaggrin was extracted from human epidermis and then partially purified. AFA in 103 cases of RA and 140 cases of controls were detected by western blot. APF and AKA were detected using indirect immunofluorescence. Results AFA test resulted in diagnosis of 35.9% of 103 RA samples, with a specificity of 93.7%. The result was significantly higher than the controls (P

[Objective]To investigate the effect of a single subcutaneous dose of an antisense oligonucleotide(ASO) on particle-induced osteolysis.[Method]The murine calvaria osteolysis model was utilized in ICR mice.Bone resorption was measured with the toluidine blue staining.Osteoclasts were detected by tartrate resistant acid phosphatase(TRAP) staining assay and were quantified by a TRAP quantification kit.[Result]Bone resorption was 0.347 ? 0.09 mm2 in animals with particle implantation,and decreased to 0.123 ? 0.05 mm2 and 0.052 ? 0.02 mm2 after ASO treatment in low and high doses,respectively.The bone resorption was reestablished in animals given an additional TNF-?.The number of osteoclasts in animal calvaria treated with ASO was reduced obviously compared with those untreated animals and the quantification results indicated that about 90% osteoclastgenesis was suprressed by the ASO.Additionally,the osteoclastgenesis was reestablished by the addition of TNF-?.[Conclusion]An antisense oligonucleotide targeting an inflammatory factor,TNF-?,has been to suppress the osteolysis induced by particle for the first time.This new finding holds a great promise.It is a therapeutic strategy for the component loosening.

Methamphetamine ( METH ) , a powerful stimulant of amphetamine type in the central nervous system ( CNS ) , is a new kind of synthetic drugs mainly in psychological dependence. Long-time abuse will lead to strong neurotoxicity and drug de-pendence. Recently it has become the abuse problem in public health. Autophagy is a process of degradation and utilization of the cellular components by the lysosome, which widely exists in eukaryotic cells. Many studies show that methamphetamine can iduce autophagy,but the underlying mechanism is not fully un-derstood yet . Therefore,the mechanisms and signaling pathways of autophagy by methamphetamine and the roles of autophagy in methamphetamine induce neurotoxicity are reviewed in this pa-per, so as to provide reference materials for further research of methamphetamine and autophagy.