Objective] To observe the use of large-connecting-meridians therapy(agitate blood) for the treatment of two cases of Guillain-Barre syndrome for clinical efficacy. [Method] Two cases of Guillain-Barre syndrome differentiation apply the principlesfrom yin to yang,from yang to yin,according to the meridians order, sequentially taking acupuncture, once daily treatment, continuous treatment for 5 days, rest for two days, surrounded by a continuous course of treatment, continuous treatment of four cycles. [Results] After stomach deficiency syndrome type of patients treated, appetite and sprint of improvement,the muscle gradually was full, muscle strength increased significantly, while blood cells and cerebrospinal fluid protein level were restored to normal range;After stagnation context type of patient treated,numbness feeling relieved a little, numbness narrowed, cerebrospinal fluid protein level was reduced to normal level;two cases of Guillain-Barre syndrome after treatment achieved better results.[Conclusion]Large-connecting-meridians method can be applied not only to stroke, used in Guillain-Barre syndrome can also get good results.

Chronic obstructive pulmonary disease ( COPD) is a group of chronic airway inflammatory disease characterized by sustained airflow limitation and pulmonary function damage.Inflammation and tissue repair repeatedly result in airway remodeling.Now the mechanisms of airway remodeling remain unclear.More and more researches indicated that epithelial mesenchymal transition ( EMT) is crucial in the airway remodeling of COPD.EMT could be induced by various cell factors and signal channels in the airway epithelium, thus the airway remodeling happened.This review systematically introduces EMT and focuses on the relationship of EMT and airway remodeling of COPD and the probable mechanisms.

BACKGROUND:Scientists began to use stem cells to treat Alzheimer’s disease since human beings achieved embryonic stem cells successful y in 1998.
OBJECTIVE:To review the research progress in stem cells treatment for Alzheimer’s disease.
METHODS:An online search of CNKI and PubMed databases was performed for articles published from 1998 to 2012 (CNKI) using key words of“Alzheimer’s disease, stem cells, neural stem cells, mesenchymal stem cells, induced pluripotent stem cells”in Chinese and English, respectively. The articles were included which related to Alzheimer’s disease, neural stem cells, mesenchymal stem cells and induced pluripotent stem cells, and for articles in the same field, those published recently or in authorized journals were selected. A total of 85 articles were searched. According to inclusion and exclusion criteria, 29 were included.
RESULTS AND CONCLUSION:The possible mechanisms of Alzheimer’s disease include differentiation of neural-like cells, secretion of nutrition factor, improvement of endogenous repair, stimulation of vascular regeneration and action on inflammatory cells. This review highlighted research status of neural stem cells, mesenchymal stem cells and induced pluripotent stem cells in the treatment of Alzheimer’s disease. There were advantages and disadvantages for al of them. The low immunogenicity and oncogenicity of neural stem cells are advantages, but limited source reduces their application. Mesenchymal stem cells also have low immunogenicity and oncogenicity, but their differentiation potential is not good as embryonic stem cells. Induced pluripotent stem cells also have many unsolved problems, but they have been regarded as promising stem cells.

Acupuncture Points ; Acupuncture Therapy ; Adolescent ; Adult ; Female ; Humans ; Joints ; injuries ; Male ; Meridians ; Middle Aged ; Sprains and Strains ; therapy ; Young Adult

This paper presents a surgical optical navigation system with non-invasive, real-time, and positioning characteristics for open surgical procedure. The design was based on the principle of near-infrared fluorescence molecular imaging. The in vivo fluorescence excitation technology, multi-channel spectral camera technology and image fusion software technology were used. Visible and near-infrared light ring LED excitation source, multi-channel band pass filters, spectral camera 2 CCD optical sensor technology and computer systems were integrated, and, as a result, a new surgical optical navigation system was successfully developed. When the near-infrared fluorescence was injected, the system could display anatomical images of the tissue surface and near-infrared fluorescent functional images of surgical field simultaneously. The system can identify the lymphatic vessels, lymph node, tumor edge which doctor cannot find out with naked eye intra-operatively. Our research will guide effectively the surgeon to remove the tumor tissue to improve significantly the success rate of surgery. The technologies have obtained a national patent, with patent No. ZI. 2011 1 0292374. 1.
Computer Systems ; Fluorescence ; Humans ; Infrared Rays ; Lymph Nodes ; Neoplasms ; Optical Imaging ; Software ; Surgery, Computer-Assisted

Objective Clinical efficacies of angiogenesis and improvement in lower limb ischemia are compared for the transplantation of both the peripheral blood stem cells(PBSC)and bone marrow stem cells(BMSC).Methods A total of 170 patients suffering from lower limb ischemia who received stem cell transplantation were monitored for random comparison study during September 2004 to October 2006.Among them 94 patients were treated with PBSC and 76 were BMSC.All the clinical data and laboratory findings before and after the transplantation in the 1st.12th and 24th weeks were comprehensively evaluated and tested by SPSS 12.0 software package.Results The total number of MNC,CD34+ cells and CD133+ cells in PBSC group was greatly more than BMSC group and had prominent or very prominent variances(P

[Objective] To explore the surgery protocol of the serious dislocation of acromio-clavieular joint,and compare the outcome of ORIF with AO/ASIF clavicle hook plate only to ORIF with eoraco-clavieular ligament reconstruction.[Method]Fity-two cases of the dislocation of acromio-clavicular joint were reviewed,Group A was ORIF only including 29 cases(male 20,female 9,mean age 45),according to the system of Rockwood,as TypeⅢ in 12 patients,TypeⅣ in 15 patients and TypeⅤ in 2 patients,the other 23 cases ORIF with eoraeo-clavieular l ligament reconstruction was Group B(male 15,female 8,mean age 50.6),according to the system of Rockwood,as TypeⅢ in 9 patients,TypeⅣ in 13 patients and TypeⅤ in 1 patients,AO/ASIF claviadar hook plates were used for all of the ORIF,and modified Weaver's method was used to reconstruct eoraco-clavieular ligament.Outcome was evaluated by Lazzcano scale after 8~12 months,and data was analyzed by SPSS 10.0.[Result]Good outcome was achieved in all of the patients,there was no significanty statistical difference between the two groups.[Conclusion]Clavicle hook plate is a kind of perfect internal fixation.Reconstruction of eoraco-clavleular ligament is not necessary if the AO/ASIF plate is used,unless serious avulsion of deltoid and trapezius muscle involved.

Objective:To explore the 5-HT mechanism of stress . Methods:The method of polymerase chain reaction (PCR) was used to detect the presence of 5-HT receptor subtypes in the hippocampus,hypothalamic and midbrain of the rat after 30days of movement restrict compared with normal cage control groups (CC) . The specific oligonucleotide primers were synthesized based on the complementary DNA sequence for each of the rat 5-HT 1A and 5-HT 2A receptor subtypes . Results : The expression of 5-HT 1A receptor subtypes in each regions of the rat brain after movement restrict were signifcantly lower than that of the CC groups, and the expression of 5-HT 2A receptor subtype were signifcantly higher( P

Objective To investigate women's psychological o utcome and its effect factors before and after hysterectomy. Methods In the clinical prospective study, 83 women were investigated for the ir psychiatric status with BDI, HAMD and SCL-90 questionnaires at the time of a dmission and 3 months after hysterectomy. Results The posto perative scales of BDI, HAMD, and SCL-90 were significantly lower than the preo perative ones. The morbidity of depression was decreased from 33.7% to 10.1%. Th e changes of SCL-90 were varied among different age groups. Before hysterectomy , HAMD scales were related negatively to women's educational level, but positiv ely to chief complaints. However, after hysterectomy, HAMD scales were related p ositively to parity, having other physical diseases, but negatively related to p atient's age. Conclusion Hysterectomy is beneficial to wom en's psychiatric and somatic health. Depression occurring before and after hyst erectomy is seen more often in women younger, having more parity, less-educate d, complaining of dysmenorrhea and/or menorrhagia and having other diseases.

Objective To observe the clinical efficacy of autologous peripheral blood stem cells (PBSC) transplantation in 26 cases of diabetic foot.Methods From 2003-11 to 2004-07,26 patients with diabetic foot received rhG-CSF 450~600ug/d by hypodermic injection for 5 days to mobilize stem cells.On the sixth day,PBSC were collected by COBE 6.1 Spectra Version for 82~148ml.The PBSC were injected into the ischemic lower limbs and foot intramuscularly for 3?3 cm distance.The clinical and laboratory findings were monitored.Results In 26 patients receiving PBSC transplantation,obvious pain-relief was shown in 23 cases for 3~14 days,foot cold-feeling changed in 24 patients after 2~7 days,foot ulcer improved in 4 cases after 4~12 weeks.ABI increased in 6 cases.Digital subtraction angiographic scores were peformed in 2 patients after 8 weeks,and there was new collateral vessels formation.No related complication or adverse effects were observed in all process.Conclusion Autologous PBSC transplantation might be a safe and effctive method for diabetic foot.