Objective To detect the expression of protein kinase C(PKC) in multidrug resistant cells of colorectal carcinoma after ionizing irradiation and observe the effect of PKC on occurrence and development of the multidrug resistant.Methods The effect of PKC on multidrug resistant HCG-8 cells of colorectal carcinoma after treated with X-ray was detected by indirect immunofluorescence technique and flow cytometry.Results Compared with sham-irradiation group,the positive percentage of PKC was increased significantly(P

Objective To investigate the surgical treatment method for senile gastric cancer complicated with portal hypertension. Methods Retrospective analysis was used for 88 elderly patients who suffered from gastric cancer complicated with portal hypertension.Among the cases,35 cases were treated with standard radical resection,38 cases treated with cytoreductive surgery,9 cases with palliative operation,and 6 cases cured with jejunal fistula.Operation complications,death and mortality,the risk factors of postoperative complications were observed. Results For all the patients,the operation time was (170±33) min,and the intraoperative bleeding was (490±100) ml.In 35 patients (39.8％) occurred operation complications,among which the incidence of incision infection and anastomotic leakage was the highest, which accounted for 14.3％ and 11.4％,respectively.In 5 years,69 patients were in death,the mortality rate was 78.4％ which most resulted from liver failure (21.7％).Logistic regression analysis showed that age,tumor diameter,operation time and perioperation bleeding were the risk factors for death ( 95％ CI:6.92-13.33,7.39-18.26,11.34 24.85,8.69-19.27). Conclusions The surgical approach for elderly gastric cancer patients complicated with portal hypertension should be chosen carefully,otherwise it will bring serious operation complications,and even endanger the lives of patients.

Objective To explore clinical effect of self-made traditional Chinese medicine for hand-foot-mouth disease.Methods 118 cases with hand-foot-mouth disease were randomly divided into two groups.The control group(n=59)were treated by regular western medicines.On that basis,the study group(n=59)were treated bys elf-made traditional Chinese medicine.And the curative effects of two groups were observed.Results Fever subsidise time of the study group after treatment was(2.0±0.7)d,which was significantly shorter than that of the control group,with P＜0.05.Rash subsidise time of the study group after treatment was(3.9±0.9)d,was significantly shorter than that of the control group,with P＜0.05.Length of hospital stay of the study group after treatment was(3.5±1.3)d,was significantly shorter than that of the control group,with P＜0.05.Total effective rate of the study group after treatment was 94.9％,which was significantly higher than that of the control group(81.4％),with P＜0.05.Conclusion Self-made traditional Chinese medicine for hand-foot-mouth disease has good clinical curative effect,has high security and reliability.

[Objective]Exploring from the specific case analysis of Chinese and western medicine treatment of primary amenorrhea with the clinical curative effect of infertility. [Method]In2013 January by Professor Xu Xiaofeng, doctor diagnosed primary amenorrhea with infertility and 1 patient of typicalcase, combined traditional Chinese medicine(TCM) and western medicine artificial menstrual cycle for 3 months, 3 months after the Chinese medicine Tiaozhou method combined with low dose of HMG with HCG inovulation induction treatment. [Result]After the treatment, the patients had pregnancy 70D, blood hormone and B Ultrasound examination were normal. [Conclusion]Combined treatment of TCM and western medicine is better than pure Chinese medicine or western medicine, suggesting that combination of TCM and western medicine is effective for the treatment of primary amenorrhea with infer-tility.

An analysis on the human resources, equipments and budgets of the township hospitals in Wenzhou City discovered the following setbacks: Changeable management system, poor positioning of their functionality, difficulty to play their roles, insufficient funding, inadequate infrastructure, slow development, lack of medical technicians, low level of medical services, and lack of development momentum. Based on these findings, the author proposed the following to upgrade their functionality and promote their development: Clearly positioning their functionality, improving the financial assurance mechanism, deepening system reforms in these hospitals, and further clarifying job divisions.

Mesenteric venous thrombosis (MVT) is a kind of rare intestinal anemic disease.Because the clinical manifestations of MVT do not have high specificity,early diagnosis is difficult.In recent years,with huge progress of medical imaging technology,particularly the clinical application of spiral CT and MRI,the early diagnosis of MVT became possible.With the development of interventional therapies,especially the maturity of the interventional treatment technology such as mesenteric artery perfusion with papaverine,thrombectomy and thrombolysis via percutaneous transhepatic portal vein and so on,MVT mortality rate reduced greatly.The diagnosis and therapy status of MVT were reviewed in this article.

Benefits in survival and quality of life have been demonstrated in patients with advanced gastric cancer receiving chemotherapy plus optimal supportive care versus optimal supportive care alone.The taxanes, which are among the most promising cytotoxic agents in clinical use have considerable activity in a number of tumor types.Paclitaxel,especially low-dose weekly administration,has shown encouraging activity in the treatment of advanced gastric cancer as a single agent.Patients treated with the low-dose weekly paclitaxel regimen had a statistically superior response rate and time to disease progression as well as clinically significant prolongation of survival.And it offers a new option for those advanced gastric patients such as the aged,poor performance status(PS) or unsuitable for combination chemotherapy,and those even with no indications for rountine chemotherapy.This review is focused on the clinical reports of low-dose weekly administration of paclitaxel for patients with advanced gastric cancer.

Purpose:To explore the development change of histopathology of the mouse forestomach neoplasm inducd by B(a)P.Methods:A part of the mice were killed each four weeks after they were given benzo(a)pyrene (concentration 5mg/ml) by lavage for four weeks, twice per week. The histopathology of the forestomach was then observed.Results:Forestomach neoplasm was noticed from the 17~(th) week in the mice. Local gland hyperplasia, arrangement disorder gradually developing into glandular abnormality hyperplasia and then transformed into early stomach cancer and finally into aggressive stomach cancer could be observed.Conclusions:Latent period of the mouse forestomach neoplasm induced by B(a)P is about three months. The development course is atrophic gastritis, atypical hyperplasia and stomach cancer.[

BACKGROUND: The incidence of cognition disturbance after cerebral infarction is about 20% -30% and effective drugs for its prevention and treatment are anticipated.OBJECTIVE: To investigate the effect of nicergoline on cognition disturbance after cerebral infarction and explore its mechanism.DESIGN: Randomized controlled trial based on patients.SETTING: Neurological department in a medical university.PARTICIPANTS: A total of 60 patients admitted to the Neurological Department of the Second Hospital Affiliated to Chongqing Medical University for cerebral infarction during October 1999 and April 2001 were recruited in this study, and randomly divided into two groups, nicergoline treatment group and control group with 30 in each.METHODS: Mini-mental state examination(MMSE) score was evaluated and cerebral blood flow was determined with transcranial Doppler' s ultrasonography within one week after admission and three months after admission, respectively, and data were analyzed with SPSS software.MAIN OUTCOME MEASURES: MMSE score and velocity of blood flow in treatment and control groups.RESULTS: MMSE score in memory, calculation and recollection decreased significantly[ (1.2 ± 1.3), (2. 2 ± 2. 1) and(1.0 ± 1.7), respectively] in control group but did not change much[ (3.9 ± 1.4), (4. 4 ± 1.9) and(4.0 ± 1.6) ]in treatment group. The velocity of blood flow in control group decreased apparently, while it increased in treatment group[(58.31 ±10. 15) and(65.79 ±9.74) cm/s in the right middle cerebral artery].CONCLUSION: Nicergoline can prevent and treat vascular cognition disturbance, and improvement of blood supply may be one of the mechanisms.

The outbreak of high-pathogenicity avian influenza viruses causing severe illness and death has severely disrupted poultry production and trade and posed a significant threat to public health.In recent thirty years,the virus has been endemic in Southeast Asia,China,Europe and Africa.Although the virus shows little ability of transmission between humans,a process of genetic reassortment could occur in a human co-infected with avian influenza A virus and a human strain of influenza A virus.So H5N1 represents a potential source of the next pandemic influenza.Direct avian-human H5N1 virus transmission is the predominant means of human infection,through direct contact with infected birds and virus-contaminated fomites or with fertilizer containing poultry feces.After several rounds of infection the viruses acquire the mutations required for crossing species barrier and infect a range of mammalian species including humans.Currently,vaccination is still the key strategy to prevent severe illness and death from pandemic influenza.Antiviral drugs such as NA inhibitors play an important role in antiviral treatment.The features of avian influenza virus and its mechanism of transmission in animals and humans are herewith reviewed,and the pathopoiesis of the virus,the current status of vaccine development and antiviral treatment are also discussed.