To improve effect of teaching, we use participation style of teaching on specified situation in rehabilitation which focuse students and the students play different roles under guidance of teachers, who analyze and make a Conclusion at last.

Objective To study clinical value of multiple organ dysfunction syndrome (MODS) score to evaluate prognosis for patients with liver failure. Methods A total of 189 patients with liver failure were recruited into the study, 125 deaths and 64 survivals. Their vital sign, platelets count (PLT), prothrombin time (PT) and international normalized ratio of PT (INRPT), fraction of inhaled oxygen (FiO2), arterial oxygen partial pressure (PaO2), serum levels of total bilirubin (TB), albumin (ALB), creatinine (Cr), pressure-adjusted heart rate (PAHR), glasgow coma score (GCS), degree of aacitic fluid (DAF) and stage of hepatic encephalopathy (SHE) were evaluated within 24 hours after admission. Each of the patients scored according to the criteria of MODS score, Child-Turcotte-Pugh (CTP) score and model for end-stage liver disease (MELD) score. Difference in MODS score between death and survival groups was compared and accuracy of prognosis of MODS score, CTP score and MELD score were evaluated by the area under receiver operating characteristic (ROC) curve. Survival time of patients in the two groups classified by their MODS scores was compared with Kaplan-Meier (K-M) survival curve. Results There was significant difference in PT, INRPT, PaO2, Cr, GCS, SHE between death and survival groups, but there was no difference in TB, ALB, PLT, FiO2, PHAR and DAF between them. Mean score of MODS in death group (9±2) was significantly higher than that in survival group (7±1) (t=9.076, P<0.01). The area under ROC curve (AUC) of MODS score (0.814) was close to that of MELD score (0.827), but higher than that of CTP score (0.714). There was significant difference in survival time between the varied groups classified by MODS score (χ2=72.451, P<0.01). Conclusions Clinical value of MODS score is equivalent to that of MELD score in evaluating prognosis for patients with liver failure, even better than that of CTP score, which can be used to evaluate short-term prognosis for patients with liver failure.

Objective To study the relationship between CA125 and endometriosis.Methods We tested the level of CA125 in serum and fluid of ovarian cysts of patients with endometriosis and non-endometriosis,respectively.Results The mean CA125 concentration in the fluid of endometrial cysts of the ovary(ECO) was remarkably higher than that of the serum in ECO group(P0.05).Conclusion The level of CA125 has bearing on the occurrence,development and prognosis of endometriosis,CA125 level is positively correlated with the severity of the disease.Therefore,it is valuable to test serum CA125 level for diagnosing endometriosis.

PGD_2 and its signal transduction way play a critical role in the pathogenesis of bronchial asthma, such as immune and allergic inflammatory responses.So more and more investigators pay attention to it.This paper reviews that PGD_2 and its recepors effect on the pathologic changes in asthma.

Objective To study the gait characteristics of spastic diplegia cerebral palsy children. Methods The gaits of 26 spastic diplegia cerebral palsy children were analyzed. Results and Conclusion There were significant differences between spastic diplegia cerebral palsy children and 20 healthy subjects in both temporal-spatial parameters (stride time, stride length, stance time (%), cadence, speed) and kinematic parameters (the joint angle of hip, knee, ankle occurring at heel-contact, toe-off, the maximal extension in stand phase, maximal flexion in swing phase and total excursion on sagittal plane).

Human mammaglobin (hMAM) is a recently discovered breast tissue specific protein with a high sensitivity and specificity. The expression of hMAM in breast cancer and its relationship with breast cancer indicate that hMAM is a novel breast specific marker that can induce cytotoxic actions from immune system and therefore may have great potentials in breast cancer treatment.

and each of the resistant strains was resistant at least to 5 antibiotics.13 strains out of the 28 harbored plasmids ranging from 1 to 6 in number and 105 Md to 2.7 Md in molecular weight.The plasmid with 50 Md was the most popular one in this study,which may be the epidemic plasmid in the urologic unit.It was also found that 2 different species of bacteria isloated from the patients with infection of the urinary tract showed identical plasmid profile,which indicates that these 2 patients may be associated with each other.

Objective:To construct a eukaryotic expression vector for wild-type human Interleukin-13(whIL-13)and its mutant(mhIL-13) gene fused with enhanced green fluorescent protein(EGFP) and to analyze the expression and subcellular location of the fusion protein in COS-7 cells.Methods:The whole whIL-13 and mhIL-13 coding gene was amplified by RT-PCR. And then the expression vector for EGFP-whIL-13, EGFP-mhIL-13 fusion protein were constructed.The recombinant fusion gene were transfected into COS-7 cells.The expression and subcellular location of the fusion protein were detected by confocal microscopy.Results:The construction of expression vector were all corrected.In the positive cells, green fluorescent was found to be localized in cytoplasm,while empty in nucleus.Conclusion:The expression vectors for EGFP-whIL-13, EGFP-mhIL-13 fusion protein were constructed successfully and expressed in COS-7 cells. Fusion proteins were distributed in cytoplasm and no significant difference was observed between them.

Objective To explore the effect of δ-opioid receptor (DOR)on ischemic tolerance of rat brain.Methods The focal ischemic tolerance models of Sprague Dawley rats were established using the twice suture method with the middle cerebral artery occlusion (MCAO).A total of 24 male Sprague Dawley rats were randomly divided into sham group,ischemia (MCAO) group,sham +ischemia (sham+ MCAO) group and ischemic preconditioning + ischemia (IP+ MCAO) group (n=6,each).The neurological status was assessed using Zea-Longa neurological deficit scores at 7 days after cerebral infarction.The mRNA expressions of DOR,Bax,Bcl-2 in hippocampus in ischemic rat brain were detected by RT-PCR method.Results The Zea-Longa neurological deficit scores were 0.0±0.0,2.6±0.5,2.8±0.6 and 1.5±0.6 in Sham group,MCAO group,Sham+MCAO group and IP+ MCAO group,respectively at 7 days after cerebral infarction.The scores had significant differences among MCAO group,Sham+ MCAO group and IP+ MCAO group (both P＜0.01),but no difference between MCAO group and Sham+MCAO group(P＞0.05).The mRNA expressions of DOR and Bcl-2 were higher and Bax mRNA expression was lower in IP+MCAO group than in MCAO and Sham+ MCAO groups (all P＜0.01).Conclusions Ischemic preconditioning may increase the mRNA expressions of DOR and Bcl-2,reduce Bax mRNA expression,and improve the neurological status in rats.

BACKGROUND:Cerebral ischemia tolerance can promote proliferation of autologous neural stem cells in the hippocampus of cerebral infarction rats, but panaxtrial saponins effects on the proliferation of autologous neural stem cells in the brain have not been reported. OBJECTIVE:To explore the relationship of panaxtrial saponins, ischemic preconditioning and proliferation of endogenous neural stem cells in the hippocampus of rats at 7 days after cerebral infarction, and to observe the effect on neurobehavioral scores of rats after cerebral infarction. METHODS:Fifty Sprague-Dawley rats were included and randomly divided into five groups:sham group, ischemia group, ischemic control group, ischemic preconditioning group, and panaxtrial saponins group. In the latter four groups, acute models of cerebral infarction were established using Zea-Longa method. In the sham group, only an incision was made on the neck. The focal-focal ischemic tolerance models were established with twice suture method in the ischemic preconditioning and panaxtrial saponins groups. Sham operation was instead of ischemic preconditioning in the ischemic control group. In the panaxtrial saponins group, rats were given intraperitoneal injection of 100 mg/kg panaxtrial saponins at 7 days before modeling. RESULTS AND CONCLUSION:After 7 days of cerebral infarction, the neurobehavioral score and the number of neural stem cells in the hippocampus were significantly increased in the ischemia group (P<0.01);compared with the ischemia group, the neurobehavioral scores were lowered in the ischemic preconditioning and panaxtrial saponins groups (P<0.01), while the number of neural stem cells in the hippocampus was increased (P<0.01). However, there was no difference between the ischemic preconditioning and panaxtrial saponins groups (P>0.05). In addition, differences in the neurobehavioral scores and the number of neural stem cells in the hippocampus were insignificant between the ischemic control group and ischemia group (P>0.05). These findings indicate that panaxtrial saponins can play a role similar to ischemic tolerance, and thus improve neurologic impairment in rats with cerebral infarction.