Objective To research the association of IR and β-cell function with MS in T2DM.Methods 116 T2DM subjects were divided into two groups:MS(n=60)and T2DM(n=56),and FIns,FPG,BP and other items were measured.Results HOMA-IR was significantly higher in MS than in T2DM and control group(P<0.05).HOMA-β was significantly lower in MS than in T2DM and control group(P<0.05).And FPG,waist circumference(WC)in MS group were positively correlated with the IR level(P<0.05).Conclusions The HOMA-IR was highest in T2DM with MS,and HOMA-β was lowest in T2DM with MS

Objective To evaluate the epidemiology, prophylaxis and treatment of bacterial infections after orthotopic liver retransplantation.Methods A retrospective study on bacterial infections in 22 patients undergoing othotopic liver retransplantation was carried out.Results Bacterial infections were confirmed in 8 of 22 preoperation and in 20 of 22 (90.9 %) postoperation. The median time of bacterial infections was 7 days after liver retransplantation. Fifty-one pathogenic organisms including 25 types were separated. The most commonly seen infected sites were biliary tract (50.0 %, 11/22), abdomen (45.4 %, 10/22), respiratory tract (36.4 %, 8/22), blood (31.7 %, 7/22), incision ((9.1) %, 2/22), bowel (4.5 %, 1/22), veins with venipuncture (4.5 %, 1/22). Seven cases of sepsis died.Conclusion The infectious rate after liver retransplantation is high and the types of bacterial disseminating loosely on organs are varied. The mortality will be increased if the patients develop sepsis. Reasonable use of immunosuppressants, selection of antibiotics and the supporting therapy are the keys to prevent and treat infections following liver retransplantation.

Objective To investigate the expression and pathological diagnostic utility of ?-methylacyl-coenzyme A racemase(P504S)in prostate cancer(PC). Methods The specimens of 42 cases of PC,18 cases of prostatic intraepithelial neoplasia(PIN)and 25 cases of benign prostatic hyperplasia(BPH)tissues were immunohistochemical stained with P504S,34?E 12 and vascular endothelial growth factor(VEGF)antibody,respectively. Their staining extent and intensity were analyzed and compared. Results Of the 42 PC cases,33(79%)showed positive P504S staining with moderate to strong staining intensity, which was significantly stronger than that of PIN and BPH cases,while adjacent normal glands were all negative. Weakly positive P504S staining was found in 6(33%)of 18 PIN cases and 2(8%)of 25 BPH cases,while it was negative in others.The specimens of BPH cases had a complete basal cell layer and were positive for 34?E 12 ;while 40 cases of PC had no basal cell layer and only 2 cases had discontinuous basal cell layers with weak staining.The basal cell layers of PIN cases were positive for 34?E 12 and the staining was a little weaker than that of BPH cases.All the specimens of BPH,PIN and PC cases showed positive staining for VEGF,the extent and staining intensity had no significant difference among these groups. Conclusions P504S has high sensitivity and good specificity in the diagnosis of PC,and is helpful for differentiating the suspected cases when combined with 34?E 12.

To explore the adhesion of peripheral blood lymphocytes and eosinophils and lung microvascular endothelial cells (LMEC) in asthma. Guinea pigs were divided into asthma model group and control group; the adhesion rates of isolated peripheral lymphocytes, eosinophils and the cultured LMEC monolayers were assayed.The mRNA expressions of VCAM 1 and eotaxin in LMEC were determined by RT PCR.The DNA binding activities of NF ?B and AP 1 of LMEC were detected by electrophoretic mobility shifts assay (EMSA). Under the stimulation with serum of asthma group:①the adhesion rates of lymphocytes, activated eosinophils and LMEC were significantly elevated( P

Aims To construct the N-terminal Strep-tagged ( NS-tagged) fusion protein expression vector, and to apply the vector to express NS-tagged fusion proteins of Chlamydia RNA polymerase subunit. Meth-ods By using PCR method, NS fusion protein tag and a new multiple cloning sites (MCS) were inserted into pET21c-DH plasmid by primers to replace the original T7 protein tag and MCS. The newly introduced Not I cutting site was chosen for self-ligation of PCR prod-uct. Then, the cyclized PCR product was transformed into DH-5α competent cells. The positive clones were selected by PCR and sequencing. To get NS-tagged fu-sion proteins of chlamydial RNA polymerase subunits, the α, β and β′ subunits were inserted between BamH I and Sal I cutting sites of the newly constructed ex-pression vector. Then, the NS-α, NS-β and NS-β′ ex-pression vectors were transformed into Arctic Express expression cells. The fusion protein expression statuses of transformed cells were identified by Commassie blue staining and Western blot. Results The NS-tagged fusion protein expression vector pET21c-NS-MCS was successfully constructed, and NS-α, NS-β and NS-β′fusion proteins were obtained by using this newly con-structed expression vector. Conclusions In this pro-ject, we constructed an NS-tagged fusion protein ex-pression vector and applied it to express NS-α, NS-βand NS-β′ fusion proteins. Our study can lay a solid foundation for the study of transcriptional regulation of Chlamydia genes.

To introduce a rehabilitation program for total hip replacement (THR), including muscle strength training; rehabilitation for scar and adhesion, scoliosis, hip control, etc. The principle and the cautions were discussed.

Objective To investigate the diagnostic significance of the relative band power(RBP) of EEG in acute focal cerebral ischemia (AFCI). Methods EEG monitoring was performed in 20 patients with AFCI (51 lesions) in neurological intensive care unit (NICU) and 20 patients with normal EEG (control group) in NICU. The changes of bilateral RBP were observed and analyzed comparatively. RBP in the infarction group was compared with that in the control group. The diagnostic capabilities of all wave-band RBPs were assessed with the receiver operating characteristic (ROC) analysis. Results AFCI shoved asymmetry on all the wave-band RBPs. The RBP of of δwave on the lesion side was significantly higher than that on the normal side (P <0.01). RBPs of α, β and θ waves were decreased significantly (P <0.05). RBP of δ wave in the infarction group was increased significantly compared to the control group (P < 0.01 ). RBPs of α and β waves were decreased significantly (P <0.01). The diagnostic accuracy of the RBP of α was the highest, and both β and δ waves also had significance for the diagnosis of AFCI. Conclusions RBP had an important significance for the early diagnosis of AFCI. Conclusions RBP had an important significance for the early diagnosis of AFCI.

Objective To analyze the correlative factors of peptic ulcer recurrence in the elderly. Methods From January to December 2009,169 elderly patients (≥ 60 years old)with peptic ulcer delected by edoscopy were enrolled,whose treatment and usage of medication were analyzed.Data of treatment and recurrence in 3-year follow-up were recorded.Mann-Whitney rank sum test and Logistic regression analysis were performed to analyze the correlated factors.Results The potential risk factors associated with recurrence of peptic ulcer in the elderly were screened and analyzed by single factor analysis,and ulcer size, ulcer location, concomitant usage of drugs, smoking and condition of Helicobacterpylori (H .pylori )infection at the end of follow-up were found to be correlated with recurrence of peptic ulcer in the elderly.After adjusting age and gender,the potential risk factors were analyzed by a Logistic stepwise regression model.Smoking (OR = 1 .788,P = 0.001 ),combined medication (OR=6.202,P =0.015 ),ulcer size (OR =2.697,P =0.032 )and condition of H .pylori infection at the end of follow-up (OR=43.784,P =0.007)were found to be correlated with recurrence of peptic ulcer in the elderly.Conclusion Smoking,combined medication,ulcer size and condition of H .pylori infection at the end of follow-up have an impact on peptic ulcer recurrence in the elderly.

OBJECTIVEthe main purpose of this study is to develop an assay for detection of skin viability in situ.

METHODSTwo nucleic acid dyes, SYTO 13 and ethidium bromide (EB), are used to assess the viability of skin tissue. The viability of cells can be determined by different colors under fluorescence microscope. In this study, the recovery of skin restored at 4 degrees C for different periods is measured by SYTO/EB dyes and a type of indirect assessment, WST-1. Then both results are compared.

RESULTSThe results measured by two methods showed that the viability of skin tissue was nearly 30% after two weeks storage. At any point of experiment, the data from two methods have not obvious variance.

CONCLUSIONThe assessment of skin viability by SYTO/EB is also quick, sensitive and convenient. At the same time the viability of any type of cells can be observed in situ. So it can be considered as an available method for detection of skin viability.

Animals ; Cell Survival ; physiology ; Cryopreservation ; Ethidium ; Male ; Models, Animal ; Rats ; Skin ; cytology ; metabolism ; Skin Physiological Phenomena ; Tissue Preservation ; methods

Aim To look for novel small-molecule inhibitors of CDK9 through structure-based virtual screening and biological activity determination.Methods Homology modeling of CDK9 was based on the 3-D structure of other cyclin-dependent kinase family members,and then virtual screening by DOCK(molecular docking)of database of small molecule was carried on.MTT method was used in inhibition of tumor cell growth in vitro,while Western blot was used for further study of molecular mechanisms.Results From the top 1000 compounds with the best DOCK energy score,27 compounds were selected for biological assay based on the diversity of chemical structure and functional group.12 of 27 selected compounds showed significantly inhibition activity on tumor cell proliferation,and only one compound in 12 with half-maximum inhibition concentration(IC50)values less than 20 ?mol?L-1 named C-21 was selected for further molecular mechanism study.The western blotting data showed C-21 compound could effectively inhibit CDK9 from phosphorylating large subunit C-terminal of RNA polymerase Ⅱ in a dose-dependent manner.Conclusions Through homology modeling,virtual screening by computer,determination of biological activity and experimental studies of molecular mechanism,a new promising lead compound targeted for CDK9 was found and confirmed.