Objective To detect the expression of protein kinase C(PKC) in multidrug resistant cells of colorectal carcinoma after ionizing irradiation and observe the effect of PKC on occurrence and development of the multidrug resistant.Methods The effect of PKC on multidrug resistant HCG-8 cells of colorectal carcinoma after treated with X-ray was detected by indirect immunofluorescence technique and flow cytometry.Results Compared with sham-irradiation group,the positive percentage of PKC was increased significantly(P

0.05).The percentage of eosinophils in induced sputum in asthmatics(16.3?7.2)% was higher than those in healthy volunteers(0.7?0.6)% and in COPD(6.5?5.4)%(P

OBJECTIVE To characterize the pathogenic distribution and drug resistance of nosocomial infection occurred in tumor hospital and therefore provide the information in rational administration of antibiotics to tumor patients complicated with infection.METHODS Flora cultivation and isolation were operated with the methods described by the National Clinical Laboratory Operational Regulations.Flora was identified with the VITEK-32 automatic identifier,and bacteria-susceptibility test was operated with Kirby-Bauer method.RESULTS Totally 1280 strains of pathogenic bacteria were isolated;they comprised 874 strains of G-bacteria and 416 strains of G+ bacteria.In this study,the isolation rates of Pseudomonas aeruginosa,Escherichia coli,Acinetobacter,Enterobacteriaceae and Klebsiella were 14.92%,14.92%,14.06%,13.91% and 10.47%,respectively;whereas that of Streptococcus Group D and Staphylococcus aureus were 24.06% and 7.70%,respectively.Both E.coli and S.aureus were much resistant to antimicrobial agents tested;while imipenem and vancomycin might be chosen for the G-and G+bacteria infection.CONCLUSIONS The bacterial spectrum and their drug-resistance characteristics in the tumor hospital are quite similar to that encountered in general hospitals.So the information on nosocomial infection control from the latter is of great importance in directing on and carrying out the same job in tumor hospital.

The multi-media teaching mode is more and more widely used in all kinds of teaching activities. This paper describes multi-media teaching and its development.It focuses on discussing the multi-media materials and their collecting methods, collecting tools as well as the common document formats.

Description of the importance of satisfaction evaluation for public hospitals,and design of the path and study methodology for such an evaluation.This evaluation pools the comments from government regulators,hospital management,hospital staff of doctors,nurses and technicians,patients or physical examination takers,and community residents at large.By means of fuzzy comprehensive evaluation,it built a comprehensive evaluation model for public hospital satisfaction,and carried out applied studies for such a model.

Objective To study the clinical curative effect of Azithromycin in the treatment of mycoplasma pneumonia.Methods 24 cases of mycoplasma pneumonia hospitalization patients were selected and made full analysis of their medical records.Results 24 acute patients successfully cured through Azithromycin with good clinical curative effect,beginning with symptoms of fevering,coughing,wheezing,and small blisters in lungs,accompanied by external pulmonary manifestations such as diarrhea,myocardial injury and positive results of serum Mb-IgM test.Conclusion Azithromycin in treatment of mycoplasma pneumonia could quickly retum temperature,and reduc hospitalized time.

ObjectiveTo investigate the onset of the measurability and the evolution of the subjective acuity in normal infant.MethodsTwo hundred and sixty - four normal infants were measured using the crowded HOTV recognition symbol system for subjective acuity and the closed-circuit operant preferential looking system(COPL) controlled by computer for grating acuity.The onset of measurability and the evolution of the subjective acuity of the normal infant sample were recorded and analyzed.ResultsIn the study part of the infants with 24-month old began to recognize the subjective vision symbols ( 26.32％ ).The measurability of subjective acuity of the infant group with 46 months old arrived to 100％.In the infants with 24-months old,the subjective acuity of the right eye was 4.86 ±0.05,left eye was 4.88 ± 0.08.In the infants with 46-months old the subjective acuity was 5.0 for both right and left eyes.According to Logistic regression analysis,the education background of the mother,and the instruction for infant showed the significant influencing factor.ConclusionThe study suggest that the crowded HOTV recognition symbol system controlled by computer provided a method to explore the onset of the subjective vision and the development of the infant.

Objectives To establish the mouse heart transplantation model and observe the effect of anti-rejection of IL-12 antibody.Methods Twenty-five mice undergoing heterotopic heart graft were divided into 4 groups:isograft one(n=5),simple homograft one(n=6),γ-globulin treated one(n=6)and IL-12 antibody treated one(n=8).Results The survival time of cardiac graft in the groups was as follows:＞ 100 days in isograft group,7.3 days in simple homograft group,7.5 days in r-globulin treated group and 15.4 days in IL-12 antibody treated group.Conclusions A stable mouse heart transplantation modd was established.IL-12 antibody could significantly prolong the survival of mouse cardiac graft and antagonize rejection of organ transplantation.

Objective To analyze the clinical effect of intracranial hematoma by drilling skull in treatment with hypertensive brain hemorrhage.Methods 188 patients with hypertensive cerebral hemorrhage were classified into control group with 94 cases and traditional group with 94 cases.The control group were treated with conservative medicine and observation group were treated with intracranial hematoma by drilling skull.The clinical efficacy and defection score of nerves function were observed and compared between the two groups.Results The total effective rate in observation group was significantly higher than that in control group(P＜0.05) while the death rate in observation group was significantly lower than that in control group (P＜0.05),Compared with pre-treatment,after treatment 2 weeks and 4 weeks,the Barthel index in two groups were significantly increased(P＜0.05).Moreover,after treatment 2 weeks and 4 weeks,the Barthel index in observation group was significantly higher than that in control group(P＜0.05).Conclusion The intracranial hematoma by drilling skull can enhance effective rate,decrease death rate and improve the ADL ability.

Objective To the expression of cytokines assay in peripheral blood and hematoma fluid of hypertensive cerebral hemorrhage,and the hemodynamic changes in brain tissue around the hematoma by using perfusion computed tomography imaging(PCTT)and immune turbidimetric analysis and analyze its influence on prognosis.Methods Fifty-six cases of hypertensive intracerebral hemorrhage patients were inspected with conventional CT and PCTT,quantitative analysis of hematoma and the contralateral corresponding area around the cerebral blood flow(CBF),cerebral blood volume(CBV),mean transit time (MTT),comparing acute and subacute phase CBF,CBV,MTT change.Peripheral blood,hematoma fluid compiement C_3 and C_4 levels were detected.Results In 56 patients with acute phase ipsilateral CBF,CBV,MTT were(19.3 4±3.1)ml/(min·100 g),(1.4 4±0.4)ml/g,(16.5 4±3.1)s,respectively,and in eontralateral were(50.3 4±3.4)ml/(min·100 g),(2.4±1.1)ml/g,(4.8±2.6)s,the corresponding index difference between the two sides were statistically significant(P<0.05).Subaeute phase ipsilateral CBF,CBV,MTY were(29.7±2.1)ml/(min·100 g),(1.9±0.6)ml/g,(8.9±2.7)s,the in contralateral were (48.2±6.3)ml/(min·100g),(2.3±0.7)ml/g,(4.7±1.6)s,the corresponding index difference between the two sides were statistically significant(P<0.05).Subacute phase CBF,CBV,MTT compared with acute phase,the difference Wag statistically significant(P<0.05).Acute and subacute phase of complement C_3 and C_4 in peripheral blood and hematoma fluid content of the differences were statistically significant(P<0.05).Acute and subaeute phase of edema ratios were 0.9 and 0.4,the difference was statistically significant (P<0.05).Conclusions The cerebral blood flow and cerebral perfusion of the brain tissue amund hematoma after the cerebral hemorrhage are decreased.PCTI Can analyze the intracerebral hemodynamic changes of the brain tissue around hematoma after the cerebral hemorrhage,which is helpful to treat hypertensive cerebral hemorrhage and estimate its prognosis.