Objective To the expression of cytokines assay in peripheral blood and hematoma fluid of hypertensive cerebral hemorrhage,and the hemodynamic changes in brain tissue around the hematoma by using perfusion computed tomography imaging(PCTT)and immune turbidimetric analysis and analyze its influence on prognosis.Methods Fifty-six cases of hypertensive intracerebral hemorrhage patients were inspected with conventional CT and PCTT,quantitative analysis of hematoma and the contralateral corresponding area around the cerebral blood flow(CBF),cerebral blood volume(CBV),mean transit time (MTT),comparing acute and subacute phase CBF,CBV,MTT change.Peripheral blood,hematoma fluid compiement C_3 and C_4 levels were detected.Results In 56 patients with acute phase ipsilateral CBF,CBV,MTT were(19.3 4±3.1)ml/(min·100 g),(1.4 4±0.4)ml/g,(16.5 4±3.1)s,respectively,and in eontralateral were(50.3 4±3.4)ml/(min·100 g),(2.4±1.1)ml/g,(4.8±2.6)s,the corresponding index difference between the two sides were statistically significant(P<0.05).Subaeute phase ipsilateral CBF,CBV,MTY were(29.7±2.1)ml/(min·100 g),(1.9±0.6)ml/g,(8.9±2.7)s,the in contralateral were (48.2±6.3)ml/(min·100g),(2.3±0.7)ml/g,(4.7±1.6)s,the corresponding index difference between the two sides were statistically significant(P<0.05).Subacute phase CBF,CBV,MTT compared with acute phase,the difference Wag statistically significant(P<0.05).Acute and subacute phase of complement C_3 and C_4 in peripheral blood and hematoma fluid content of the differences were statistically significant(P<0.05).Acute and subaeute phase of edema ratios were 0.9 and 0.4,the difference was statistically significant (P<0.05).Conclusions The cerebral blood flow and cerebral perfusion of the brain tissue amund hematoma after the cerebral hemorrhage are decreased.PCTI Can analyze the intracerebral hemodynamic changes of the brain tissue around hematoma after the cerebral hemorrhage,which is helpful to treat hypertensive cerebral hemorrhage and estimate its prognosis.

BACKGROUND:Spinal cord injury can cause the motor, sensory and autonomic dysfunction below the damaged surface, so the repair of spinal cord injury has been the problem of neuroscience research. OBJECTIVE:To analyze the mechanism, experimental research and clinical application of olfactory ensheathing cel transplantation for the repair of spinal cord injury, and to investigate the effect of olfactory ensheathing cel transplantation on neurological function recovery. METHODS:The basic experimental and clinical researches on olfactory ensheathing cel transplantation for the repair of spinal cord injury were retrospectively analyzed, in order to analyze the relationship between the number and time of the survival cells and the structure and function of the damages spinal cord after olfactory ensheathing cel transplantation. The inclusion and exclusion criteria of the included clinical researches, and the sources, types and transplantation method of the cells used for transplantation were clearly defined, then the effective and objective evaluation criteria was established. RESUTLS AND CONCLUSION:The animal experiments of olfactory ensheathing cel transplantation for the repair of spinal cord injury have achieved satisfactory effects. The spinal cord function score and the sensory and motor function were significantly improved compared with those before transplantation (P<0.001). The successful animal experiment could provide basis for the clinical experiment and application. Parts of the clinical experiments were fol owed-up for 5 years. Because of a smal amount of the cases in the clinical research and the short fol ow-up period, the final recovery of large number of cases cannot judged yet, further observation and research were required. The great significance of the research result is to help to find a reasonable strategy that can make the olfactory ensheathing cells displayed repairing effect sufficiently.

Objective To investigate the surgical treatment method for senile gastric cancer complicated with portal hypertension. Methods Retrospective analysis was used for 88 elderly patients who suffered from gastric cancer complicated with portal hypertension.Among the cases,35 cases were treated with standard radical resection,38 cases treated with cytoreductive surgery,9 cases with palliative operation,and 6 cases cured with jejunal fistula.Operation complications,death and mortality,the risk factors of postoperative complications were observed. Results For all the patients,the operation time was (170±33) min,and the intraoperative bleeding was (490±100) ml.In 35 patients (39.8％) occurred operation complications,among which the incidence of incision infection and anastomotic leakage was the highest, which accounted for 14.3％ and 11.4％,respectively.In 5 years,69 patients were in death,the mortality rate was 78.4％ which most resulted from liver failure (21.7％).Logistic regression analysis showed that age,tumor diameter,operation time and perioperation bleeding were the risk factors for death ( 95％ CI:6.92-13.33,7.39-18.26,11.34 24.85,8.69-19.27). Conclusions The surgical approach for elderly gastric cancer patients complicated with portal hypertension should be chosen carefully,otherwise it will bring serious operation complications,and even endanger the lives of patients.

The author analyzed the present human resources at logistics departments of public hospitals,pointing out the way out for HR innovation,and probing into the practices of changing the management philosophy,in an effort of building a complete hospital logistics HR system,and developing an excellent hospital logistics team.

Objective To investigate feasibility, efficacy and safety of holmium laser lithotripsy under ureteroscopy to treat ureteral calculi. Methods Clinical data of 162 patients with ureteral calculi treated by holmium laser lithotripsy under ureteroscopy in our hospital from June 2006 to October 2009 were reviewed retrospectively. Stone-broken rate,lithagogue rate and complication were observed. Results The overall rate of successful fragmentation for all levels of ureteral calculi in a single procedure achieved 96. 30% (156/162). The success rates of fragmentation for the upper and mid-lower segment of ureteral calculi was 86.11% (31/36)and 99.21% (125/126),respectively. In 156 patients, the postoperative follow-up of 2- 4 weeks revealed that the stone free rate was 98.08 % (153/156). The mean operative time was (36 ± 11 )mains ( ranged from 15 to 90) and hospital stay ranged from 1 to 10 days. Conclusion Holmium laser lithotripsy under ureteroscopy is feasible,high effective and safe for the treatment of ureteral calculi, especially for those with middle or lower levels ureteral calculi.

Objective To explore the related factors of plasma superoxide dismutase (SOD) levels in elderly patients with coro‐naryheartdisease.Methods 232elderlypatientswithcoronaryheartinthehospitalwereenrolledinthestudy,thepatientswhose plasma SOD≥129 U/mL were included as group A ,plasma SOD levels <129U /mL were divided into group B ,the difference ob‐served two groups were compared for each observation indicators .Results The two groups in age ,CRP ,TXB2 ,6‐Keto‐PGF1a were statistically significant different(P<0 .05) ,while in the gender composition ,ALT ,TG ,HDL ,LDL ,TC ,Cr ,Hcy was no significant difference(P>0 .05);the proportion of patients with stroke ,bacterial pneumonia/acute exacerbation of chronic bronchitis in the two groups were statistically significant different(P<0 .05);in the proportion of hypertension ,type 2 diabetes ,cancer was no statistical‐ly significant difference(P>0 .05);Non conditional Logistic regression results shown that the main factors that led to the decrease of SOD in elderly patients with coronary heart disease were age increase ,CRP increase ,TXB2 increase ,6‐Keto‐PGF1a decrease , stroke ,bacterial pneumonia/acute exacerbation of chronic bronchitis .Conclusion Old age ,elevated CRP ,elevated TXB2 ,decreased 6‐Keto‐PGF1a ,stroke ,bacterial pneumonia/acute exacerbation of chronic bronchitis are the factors leading to the decrease of plasma SOD in aged patients with coronary heart disease .

The multi-media teaching mode is more and more widely used in all kinds of teaching activities. This paper describes multi-media teaching and its development.It focuses on discussing the multi-media materials and their collecting methods, collecting tools as well as the common document formats.

OBJECTIVE: To establish the preparation process and TLC for Rutong Capsules. METHODS: The preparation process was optimized by orthogonal experiment design. The components in the preparation were identified qualitatively by TLC, and the quality standard of the preparation was established. RESULTS: Radix Angelicae Sinensis, Fructus Aurantii, Radix Bupleuri were able to be identified by TLC, and the physical and chemical characteristics of Poria and Rhizoma Atractylodes macrocephala were able to be identified. CONCLUSION: The method adopted in the study is simple, accurate and reproducible, and it is applicable for the preparation and TLC of Rutong Capsules.

OBJECTIVE:To optimize the technologic conditions for separation and purification process of Astragaloside Ⅳ using macroreticular resins.METHODS:The optimal extraction process and purification technology using macroreticular resins were investigated by orthogonal experiment design with extract yield and content of Astragaloside Ⅳ as evaluation indexes,and the influencing factors were also investigated.RESULTS:The optimal conditions for extraction were as follows:sixfold amount of water was added to extract Astragaloside Ⅳ for 3 times with 1 h each time.The isolation and purification of Astragaloside Ⅳ were influenced by relative density,elution dosage and velocity.CONCLUSION:The established optimal technologic conditions for the extraction,separation and purification of Astragaloside Ⅳ using macroreticular resins proved to be feasible.

Objective:To observe the prevention of glutathione(GSH)against chronic liver injury in rat.Methods:The chronic liver injury model was made by intracutaneous injection of carbon tetrachloride twice a week in rats for 24 times.From the 8th week after the carbon tetrachloride injection,GSH was given by oral or peritoneal administration once a day for 60 days.The animals were sacrificed at the end of the experiment.The serum level of ALT,AST were measured.The liver tissue was studied with histopathological or immunohistochemical assay.Results:GSH markly decreased the serum level of ALT,AST and improved liver tissue inflammation and lessend the liver fibrosis in histopathology compared with the model group.In the immunohistochemical stain,the deposition of type Ⅰ collagen of liver tissue significantly decreased in GSH treatment group compared with the model group.Conclusion:GSH can protect live from injury induced by toxic material.