Objective:To investigate the proliferation inhibitory effect of curcumin on human ovary cancer cell line SKOV 3 and the mechanisms.Methods:The MTT method was used to assay the biologic activities of curcumin at different time and in different doses.The cell cycle distribution was detected by flow cytometric analysis.The cell ultrastructure was observed by electron microscope.The expression of apoptosis-associated genes protein was detected with immunohistochemical technique.Results:Curcumin could effectively inhibit SKOV 3 in a dose- and time-dependent manner.IC 50 of curcumin to SKOV 3 was 79.96?mol/L in 72 hours.The cell growth was arrested at G 2/M stage.Curcumin could lead to apoptosis.When treated with curcumin for 72 hours,the expression of MTP53 and Bcl-2 were down-regulated,while the expression of Bax and Fas were up-regulated.Conclusion:Curcumin can inhibit the proliferation of SKOV 3.The apoptosis is related to the down-regulation of MTP53 and Bcl-2 and up-regulation of Bax and Fas.

Objective:To evaluate the clinical profile,treatment methods,and prognosis for malignant germ cell tumors of the ovary.Methods:A total of 38 patients with malignant ovarian germ cell tumors(15 yolk sac tumors,10 dysgerminomas,9 immature teratomas,and 4 mature cystic teratomas with malignant transformation)were analyzed retrospectively.Results:The median age of the patients was 25 years(range 11～54).There were 18 FLGO stage Ⅰ tumors (47.4%),7 stage Ⅱ and Ⅲ tumors respectively(18.4%),2 stage Ⅳ tumors (5.3%),and 4 recurrent tumors (10.5%).The overall 2-year survival rate was 76.3%,the survival rate of patients with stageⅠ-Ⅱdisease was 96.0%.Of the cases who preserved fertility,6 patients had the desire of fertility,4 healthy live births were recorded.Conclusion:The cure rate of malignant ovarian germ cell tumors has improved greatly.The staging and treatment approaches are important prognostic factors.Conservative surgery and preservation of fertility are feasible.

Objective To explore the perioperative nursing of patients with refractory paroxysmal positional vertigo treated with posterior semicircular canal occlusion. Method A retrospective analysis was performed to the experience of nursing 18 patients with refractory paroxysmal positional vertigo treated with posterior semicircular canal occlusion.Results The treatments for 17 cases were effective.Two of them had postoperative vertigo and then cured after further treatments.During one year follow-up,17 of them reported no occurrence of vertigo except only one care reporting paroxysmal positional vertigo. Conclusion Preoperative mental care and postoperative observation of the disease are critical for the enhanced curative effects.

Objective To explore the effect of psychological intervention with pantoprazole sodium in treatment of gastric ulcer. Methods A total of 80 patients with gastric ulcer from March 2015 to January 2017 were randomly divided into two groups, control group and observation group; Two groups were treated with pantoprazole sodium, the control group received conventional care, while the observation group was given psychological care.The rehabilitation of the two groups was observed and compared . Results The effective rate of observation group was 92.5％, significantly higher than that of the control group(77.5％); The satisfaction rate of the observation group was 97.5％, significantly higher than that of the control group 72.5％; the differences between the two groups were statistically significant.Conclusion The implementation of psychological nursing of pantoprazole in treatment of gastric ulcer in the process, can consolidate the curative effect to be fully reflected, help patients recover in time, and improve patient care for acceptance,it is worthy of reference.

Objective　To determine serum true insulin (TI) and proinsulin (PI) levels in patients with coronary heart disease (CHD), and to explore the relationships of them to other cardiovascular risk factors. Methods　Highly specific ELISA assays for TI and PI and RIA for immunoreactive insulin (IRI) were used. The fasting serum IRI, TI, PI levels in 30 nondiabetic patients with CHD and 30 healthy, age-matched control subjects were investigated. And the fasting and 2h postprandial plasma glucose, serum lipids and blood pressure were also determined in each individuals. Results　The levels of serum IRI, TI and PI were significantly higher in patients with CHD than those in controls (P＜0.05), while the value of insulin sensitivity index (ISI) was lower in patients with CHD than in controls (P＜0.05). There were significant independent correlations between TI, PI and age, BMI, plasma glucose, serum lipids. Conclusion Nondiabetic patients with CHD have hyperinsulinemia and insulin resistance and there is an independent association between proinsulin and dyslipidemia.

Objectives To investigate the effect of different physical activities on the blood pressure in elderly patients with hypertension,and to provide evidence for the prevention and treatment of hypertension.Methods 256 elderly patients with grade 1,2 hypertension and no other complications,and 256 healthy elderly people were selected as the control subjects.According to the blood pressure and the level of physical activity,the patients were divided into hypertension combined with low physical activity group(HL group),hypertension combined with middle physical activity group(HM group),normal blood pressure combined with low physical activity group(NL group)and normal blood pressure combined with middle physical activity group (NM group).The changes of blood pressure were compared in quiet,during and after physical activity.Results The diastolic blood pressure(DBP) and systolic blood pressure(SBP) measured at rest,peak exercise and 5 minutes after exercise were significantly higher in group HL and HM group than in NL group and NM group,and the differences between groups were statistically significant(SBP:F=11.846、18.337、10.976,all P＜ 0.001 for all;DBP:F=13.182、11.694、11.453,all P＜0.05).The DBP and SBP in HL group at rest and 5 minutes after exercise were significantly higher than those in the HM group,and the difference was statistically significant(P＜0.05).The change magnitudes of DBP and SBP were significantly lower in HL group than in the HM group,and the differences were statistically significant (P ＜ 0.05).Conclusions Taking part in an appropriate exercise can improve blood pressure level and a recovery capability of post-exercise blood pressure in elderly patients with hypertension.

Objective To investigate the relationship between interleukin (IL)-17 and IL-10 in psoriasis.Methods The expression of IL-17 and IL-10 was measured by enzyme-linked immunosorbent assay in 32 patients with psoriasis (psoriasis group) and 30 normal controls (control group).The correlation of IL-17 and IL-10 was analyzed.Results The expression of IL-17 in psoriasis group was higher than that in control group[(57.59 ± 11.99) ng/L vs.(33.27 ± 6.49) ng/L] (t =9.84,P ＜ 0.01).The expression of IL-10 in psoriasis group was lower than that in control group [(25.38 ± 4.32) ng/L vs.(32.01 ± 5.54) ng/L] (t =5.27,P＜ 0.01).There was negative correlation between IL-17 and IL-10 in patients with psoriasis (r =-0.70,P ＜ 0.01).Conclusion The pathogenesis of psoriasis may be related to imbalance of IL-17 and IL-10.

Objective To study the feasibility of the ultrasonic scalpel in the management of cystic blood vessels during laparoscopic cholecystectomy(LC). Methods Abdominal blood vessels of two dogs were managed by ultrasonic scalpel under the conditions of two step output power,low tension,low holding force and blunt scalpel head.The blood vessels were sealed and arteries and veins of (1～11)mm were cut.On this basis,the technique was applied to the management of cystic vessels during laparoscopic cystic and biliary operation. Results The sealing and cutting effect was good for the blood vessels of less than 9mm 140/150(93 3%).No blood oozing was found at the cutting ends of blood vessels during and after operation.In all 706 cases of laparoscopic gallbladder and biliary duct operations,no bleeding was found at the cutting ends of blood vessels during and after operation. Conclusions Ultrasonic scalpel can be directly used to seal and cut cystic artery during laparoscopic cholecystectomy.

Objective To assess the clinical effect of colpomyomectomy.Methods 79 hysteromyoma patients who had indication of myomectomy were randomized into two groups.One group(42 patients) received colpomyomectomy,the other group(37 patients) received celiomyomectomy.Observing the clinical effect of intraoperation and postoperation between the two groups respectively.Results Comparing the colpomyomectomy group and celiomyomectomy group,the former operation time reduced(P

Objective To establish the best extraction technology of Zhike oral liquid.Methods Using orthogonal experiment method, total flavonoids and baicalin as evaluation index, extraction time, solvent times, extraction times as factors, the optimal extraction process parameters were selected.Results The best extraction process of Zhike oral liquid:extraction time was 1h, solvent amount was 10 times, 2 times of extraction.Conclusion The extraction process is feasible, the test result is accurate and reliable, which can provide the experimental basis for the extraction process of Zheke oral liquid.