Tetraspanins are a class of cell surface glycoproteins that are expressed very broadly, which can form a complex tetraspanins net by combining with many kinds of cell surface molecules such as integrins, signal proteins, growth factors and so on.In recent years, more and more studies suggest that tetraspanins are closely related to the invasion and metastasis of malignancies and show a certain clinical value, which can be used as new diagnosis and prognosis indicators of malignancy.

Objective To observe the manifestations of adult adrenal glands in intraoperative ultrasound(IOUS).Methods A total of 71 patients who underwent surgery for liver,biliary or pancreatic lesions were enrolled.Adrenal glands manifestations in IOUS were observed,and the displaying rate and the measured thickness of adrenal glands of IOUS were compared with those of CT/MRI.Results Adult adrenal gland manifested as bilateral weak echogenic structures with clear boundary and irregularly shape in IOUS.CT/MRI displaying rate of adrenal gland was 97.18％(138/142),of IOUS was 95.07％(135/142).IOUS displaying rate of the left side adrenal gland was 97.18％(69/71),of the right side adrenal gland was 92.96％(66/71),including 4 adrenal glands not shown on CT/MRI.The thickness of adrenal gland measured with IOUS was greater than those with CT/MRI(all P＜0.05).Conclusion IOUS of Adult adrenal glands present as bilateral weak echogenic structures with clear boundary and irregularly shape.IOUS displaying rate of adult adrenal gland was higher of the left side than the right side.

Ubiquitin specific protease 33 (USP33) is a multifunctional protein regulating diverse cellular processes. The expression and role of USP33 in lung cancer remain unexplored. In this study, we show that USP33 is down-regulated in multiple cohorts of lung cancer patients and that low expression of USP33 is associated with poor prognosis. USP33 mediates Slit-Robo signaling in lung cancer cell migration. Downregulation of USP33 reduces the protein stability of Robo1 in lung cancer cells, providing a previously unknown mechanism for USP33 function in mediating Slit activity in lung cancer cells. Taken together, USP33 is a new player in lung cancer that regulates Slit-Robo signaling. Our data suggest that USP33 may be a candidate tumor suppressor for lung cancer with potential as a prognostic marker.
Blotting, Western ; Cell Line, Tumor ; Cell Movement ; genetics ; physiology ; Cohort Studies ; Down-Regulation ; Female ; Gene Expression Regulation, Neoplastic ; HEK293 Cells ; Humans ; Immunohistochemistry ; Intercellular Signaling Peptides and Proteins ; metabolism ; Kaplan-Meier Estimate ; Lung Neoplasms ; genetics ; metabolism ; pathology ; Male ; Middle Aged ; Nerve Tissue Proteins ; metabolism ; Prognosis ; RNA Interference ; Receptors, Immunologic ; metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; Signal Transduction ; genetics ; physiology ; Ubiquitin Thiolesterase ; genetics ; metabolism

Objective:To explore the mediating role of interdisciplinary cooperation ability between the level of hospice care practice and difficulty degree of hospice care for medical staff, in order to improve the quality of hospice care services for medical staff, and to provide evidence for relevant managers to formulate effective measures to reduce the implementation of hospice care difficulties.Methods:This study was a cross-sectional survey. Using convenience sampling method, the general data questionnaire, Palliative Care Difficulties Scale (PCDS), Palliative Care Self-Report Practice Scale (PCPS) and Modified Index of Interdisciplinary Collaboration (MIIC) were used to investigate 362 medical staff in all hospice care institutions of Shiyan City from July to August 2022.Results:The total score of PCDS in 362 medical staff was (40.58±13.44) points, (67.47 ± 12.50) points for PCPS and (108.36 ± 21.46) points for MIIC. There was a positive correlation between the total score of MIIC and PCDS ( r=0.500, P<0.01). The total score of PCPS was negatively correlated with the total score of MIIC ( r=-0.337, P<0.01) and the total score of PCDS ( r=-0.189, P<0.01). The interdisciplinary cooperation ability of medical staff in hospice care had a complete mediating effect between the level of practice and the degree of difficulty (Effect value=-0.190, 95% CI-0.274 to -0.126), and the mediating effect accounted for 98.5% of the total effect value. Conclusions:The practice level of hospice care can not directly affect the implementation of the difficulty degree, but by taking active measures to improve the interdisciplinary cooperation ability, so as to enhance the practice level, and then reduce the implementation of hospice care difficulty degree.

BackgroundBipolar disorder is a severe mental disorder characterized by cycling between mania/hypomania and depression， yet its underlying pathophysiological mechanism remains unclear. Several prior studies have suggested a potential role for voltage-gated calcium channel subunit genes in the etiology of bipolar disorder， particularly in their influence on brain structure. ObjectiveTo investigate the differences in grey matter volume （GMV） for individuals with bipolar disorder compared to healthy controls， and to explore the potential influence of calcium channel voltage-dependent T-type α₁ G subunit （CACNA1G） rs757415 polymorphism on GMV in bipolar disorder and clarify the specific brain regions associated with this genetic variation， thus offering a new opportunity to gain insight into the pathophysiological mechanism of bipolar disorder. MethodsA cohort of 289 patients who met the Diagnostic and Statistical Manual of Mental Disorders， fourth edition （DSM-IV） criteria for bipolar disorder were selected for participation. These patients were either admitted to hospital or examined in outpatient clinic for bipolar disorder at the Mental Health Center of West China Hospital， Sichuan University between September 2013 and December 2022. Another 322 healthy individuals were concurrently recruited as a control group from Sichuan University and surrounding communities. All participants underwent brain imaging using a 3.0 T magnetic resonance scanner to acquire data on GMV. Additionally， the presence of the CACNA1G rs757415 polymorphism was validated using the imLDRTM technique. Spearman correlation analysis was utilized to investigate potential relationship between abnormal brain regions identified through GMV data and clinical characteristics of the patients. Then the genotype-by-diagnosis interaction effect for CACNA1G rs757415 on GMV was observed using the full factor method. ResultsThe study successfully enrolled 173 patients with bipolar disorder and 207 healthy controls who completed all the necessary procedures. Analyses revealed decreased GMV for patients with bipolar disorder compared to healthy controls in the left cerebellar declive extending to cerebellar anterior/posterior lobe， fusiform gyrus， parahippocampal gyrus and inferior occipital gyrus （t=5.664， P<0.05）； in the right cerebellar anterior/posterior lobe， fusiform gyrus， parahippocampal gyrus extending to lingual gyrus （t=4.583， P<0.05）； in the bilateral anterior cingulate/paracingulate gyri， superior frontal gyrus and precuneus （t=7.543， P<0.05）； in the left lingual gyrus and superior temporal gyrus （t=6.593， P<0.05）； and in the right insula entending to central operculum （t=7.153， P<0.05）. Correlation analysis indicated that the duration of bipolar disorder was positively correlated with cerebrospinal fluid volume （r=0.258， P=0.003）， whereas negatively correlated with the GMV in the left cerebellar declive extending to cerebellar anterior/posterior lobe， inferior occipital gyrus and parahippocampal gyrus （r=-0.204， P=0.019）， in the right cerebellar anterior lobe extending to right cerebellar posterior lobe， fusiform gyrus， parahippocampal gyrus and lingual gyrus （r=-0.238， P=0.006）， in the bilateral superior frontal gyrus extending to anterior cingulate/paracingulate gyri and precuneus （r=-0.219， P=0.012）， in the left lingual gyrus extending to superior temporal gyrus （r=-0.296， P=0.001）， and in the right insula extending to central operculum （r=-0.257， P=0.003）. A significant genotype-by-diagnosis interaction effect for CACNA1G rs757415 on GMV was observed in the right parahippocampal gyrus - fusiform gyrus - cerebellum 4-5 （F=19.967， P<0.05） . In the control group， individuals carrying the non-risk allele showed increased GMV in the right parahippocampal gyrus - fusiform gyrus - cerebellum 4-5 compared to those carrying the risk allele. In contrast， within the patient group， risk allele carriers exhibited increased GMV in the same brain regions when compared to non-risk allele carriers. Moreover， the GMV in the right parahippocampal gyrus - fusiform gyrus - cerebellum 4-5 of patients with bipolar disorder carrying risk alleles was increased compared to healthy controls. ConclusionCACNA1G rs757415 polymorphism may affect the GMV in the right parahippocampal gyrus， fusiform gyrus and cerebellum 4/5 of patients with bipolar disorder. ［Funded by National Natural Science Fundation of China， （number， 82071524）］

【Objective】 To establish a dry fluorescent luminescence method for the detection of antibodies to hepatitis C virus (HCV) and evaluate its clinical application. 【Methods】 Anti-HCV antibody was detected by double-antigen sandwich dry fluorescent luminescence method established using multi-epitope chimeric antigen. The established method was used to detect national reference samples(positive 20, negative 20), and a total of 349 clinical samples, including 108 HCV patients, 36 patients with other diseases and 205 healthy individuals, which were tested in parallel with enzyme-linked immunoassay (ELISA) to evaluate the performance of the established method. 【Results】 The concordance rate of positive and negative(each 20) reference samples were both 100% (20/20), and the CV of precision reference sample was 9.16%, which met the requirements of national reference samples. In clinical performance evaluation, the AUC value was 0.984, and the sensitivity and specificity of the dry fluorescent luminescence method were 96.30% (104/108) and 96.27% (233/241). The overall concordance rate between dry fluorescent luminescence method and ELISA was 97.71% (341/349) (Kappa=0.952). 【Conclusion】 The dry fluorescence luminescence method of HCV antibody is simple and rapid, with high sensitivity and high specificity, and can be used in clinical application.

Retrograde adeno-associated viruses (AAVs) are capable of infecting the axons of projection neurons and serve as a powerful tool for the anatomical and functional characterization of neural networks. However, few retrograde AAV capsids have been shown to offer access to cortical projection neurons across different species and enable the manipulation of neural function in non-human primates (NHPs). Here, we report the development of a novel retrograde AAV capsid, AAV-DJ8R, which efficiently labeled cortical projection neurons after local administration into the striatum of mice and macaques. In addition, intrastriatally injected AAV-DJ8R mediated opsin expression in the mouse motor cortex and induced robust behavioral alterations. Moreover, AAV-DJ8R markedly increased motor cortical neuron firing upon optogenetic light stimulation after viral delivery into the macaque putamen. These data demonstrate the usefulness of AAV-DJ8R as an efficient retrograde tracer for cortical projection neurons in rodents and NHPs and indicate its suitability for use in conducting functional interrogations.
Animals ; Haplorhini ; Axons ; Motor Neurons ; Interneurons ; Macaca ; Dependovirus/genetics* ; Genetic Vectors