Objective To assess radiation dose and image quality of chest CT examinations that acquired with Flash scan technique combined with iterative reconstruction.Methods Thirty continuous patients required no-contrast chest CT with Flash scan mode and contrast enhanced chest CT with conventional CT scan mode.For each examination,the images were reconstructed by filter back projection (FBP) and sinogram affirmed iterative reconstruction (SAFIRE).The volume CT dose index (CTDIvol),dose-length product (DLP),and effective dose (ED) were estimated.The image noise,signal to noise ratio (SNR),overall subjective image quality,and respiratory motion artifacts were evaluated blindly by two radiologists.Results For Flash scan and conventional scan,SAFIRE reduced the image noise 35％ and 16％,respectively;and increased SNR 21.9％ and 25％ compared to FBP,respectively (P ＜0.05).There was significant lower subjective image scores acquired by SAFIRE than by FBP.Flash scan reduced the CTDIvol (mGy) 40.9％,DLP 32.1％,and ED 26.9％ compared to conventional CT scan mode.The motion artifact scores were reduced significantly with Flash scan mode compared to conventional scan mode.Conclusions Flash scan technique of the chest can obtain image with minimum respiratory motion artifact due to dramatically increased scan speed,and when SAFIRE was combined with,it reduces significantly radiation dose and image noise.

Objective To determine the role of valproate in the expression of SCN3A and provide new evidence for the clinical treatment of epilepsy. Methods We treated the N1E-115 cells with sodium valproate and examine the expression of SCN3A mRNA. We also constructed the expression vector of the SCN3A gene promoter then transfected the vector into N1E-115 cells ,respectively detected the level of SCN3A mRNA and the expression level of firefly luciferase of N1E-115 cells with valproate treated and untreated. Results Valproate inhibited the expression of SCN3A significantly including the level of SCN3A mRNA and luciferase protein. Conclusion Valproate inhibits the seizures by reducing the expression of the SCN3A, which provides a new understanding of the treatment of epilepsy.

Objective To discuss the effect of the modified structure of oxygen equipment on oxygen inhalation effect of patients receiving oxygen therapy.Methods The structure of oxygen equipment was modified.and 80 patients who required oxygen therapy were divided into the modified group and the non-modified group according to odd and even number of hospital admission sequence randomly with 40 patients in each group.The modified group used the modified oxygen equipment,the non-modified group used traditional one.The differences of respiration,heart rate and blood oxygen saturation rate between the two groups before oxygen inhalation,30 minutes and 2 hours after oxygen were compared.Results There was no significant difference in the respiration,heart rate and blood oxygen saturation rate between the two groups.Conclusions The modified oxygen equipment doesn't affect the oxygen therapy effect of patients,besides,it avoids the problems of pollution and the complicated disinfection procedure of the structure of the humidifier bottle,fluid and breathing vessel of the traditional equipment.

Objective: To prepare the compound Yuxingcao dispersible tablets and investigate the dissolution of baicalin in vitro. Methods: The formulation of dispersible tablets was optimized by an orthogonal design test in terms of disinterating time.Results: The optimal disintegrants were composed of CMS-Na 5%,MCC 20%,L-HPC 10%,and disintegration time of prepared compound Yuxingcao dispersible tablets followed the quality specification of dispersible tablet in Chinese Pharmacopoeia 2005. The in vitro dissolution test indicated that more than 95% of baicalin dissolved in 3 minute from dispersible tablets. Concolusion: The formulation and preparation process of compound Yuxingcao dispersible tablets were simple and feasible.

Objective To investigate the protective effect of Limb ischemic postconditioning ( LIPostC) on blood brain barrier (BBB) and explore the influence on the the expression of tight junction protein claudin-5,occludin in cerebral ischemia-reperfusion injury of mice.Methods Ninety male CD1 mice were divided into three groups randomly:Sham-operated group(sham group), ischemia-reperfusion group(I/R group) and LIPostC group.Mouse middle cerebral artery cerebral ischemia-reperfusion model was established by modified-occlusion method. Neurological deficit scores, brain water content, infarct volume, BBB dysfunction were measured at 24 h after reperfusion.Western blot and RT-qPCR were used to analyze the expressions of claudin-5, occludin.Results Compared with I/R group, neurological deficits scores were significantly decreased, brain edema was relieved, infarct volume was reduced, and the expression of claudin-5, occludin up-regulated, the differences were statistically significant(all P<0.05).Conclusion LIPostC can protect the BBB of cerebral ischemia-reperfusion injury mice by increasing the expression of claudin-5,occludin, and that will provide a theoretical basis for its clinical application.

Objective To study the distribution of the sulfur dioxide (SO2) in the different organs of mice after exposed to SO2. Methods The Kunming mice were exposed to SO2 at different doses in a chamber in which the concentration of SO2 could be monitored. The concentrations of sulfite in livers, kidneys, spleens and testis from male mice were determined by high performance liquid chromatography (HPLC) with fluorescence detection (FD). After reduction, pre-column derivation to tissue homogenates of livers, kidneys, spleens and testis, the mixture was centrifuged, and 5 ?l of the resulting supernatant was directly injected into HPLC with analytical column C18(200 mm ? 5.0 mm, 5 ?m), where the mobile phase consisted of a mixture methanol-water(contained 0.25% acetic acid ) (10:90,V/V), pH was 2.8 adjusted with phosphoric acid, and the exciting and emitting wavelength of fluorescence detection were 392nm and 479nm,respectively. The mobile phase was pumped at a flow rate of 1.0 ml/min, and the column temperature was 30 ℃. Results The standard curve of sulfite was linear in the range from 0.063 ?g/ml to 1.26 ?g/ml (r=0.997 8), and the minimal detectable concentration was 0.05 ?g/ml (S/N=3) with a recovery rates of 95%-101%. The relative standard deviations (RSD) of intra-day and inter-day RSD were less than 10%. The results showed that sulfite concentration in all organs tested from mice in SO2-exposed groups was significantly increased (P

Objective To evaluate the role of contrast enhanced ultrasound in treatment of ultrasound guided high intensity fo‐cused ultrasound ablation for uterine fibroids .Methods Forty eight women with uterine fibroids were allocated into contrast en‐hanced group(25cases ,42fibroids) and non contrast enhanced group(23 cases ,35 fibroids) .The average volume of fibroids and the non perfused volume and the fractional ablation and adverse effects between the tow groups were compared .Results Non perfused volume was observed in all fibroids of the two groups after high intensity focused ultrasound ablation through sulphur hexafluoride micro bubbles .The average non perfused volume was 19 .5(18 .2)cm3 and 22 .5(14 .5)cm3 ,fractional ablation was (78 ± 18)% and (87 ± 13)% of the uterus immediately after HIFU respectively .The average non perfused volume was 18 .6(18 .2)cm3 and 19 .5 (18 .7)cm3 ,fractional ablation was (85 .7 ± 15 .2)% and (87 .6 ± 8 .0)% of the uterus two days after HIFU .There was no signifi‐cant difference was observed in average non perfused volume and the fractional ablation between the two groups(P>0 .05) .During HIFU procedure ,more patients in non contrast enhanced group complained sacral pain than those in contrast enhanced group(P<0 .05) .Conclusion Contrast enhanced ultrasound is an accurate method in assessing the treatment effect of HIFU and could be safely used during HIFU treatment for uterine fibroids ,shorten therapy time ,and decrease side reaction .

Objective To detect the serum levels of carbohydrate antigen 199(CA199),carbohydrate antigen 125 (CA125)and carcino-embryonic antigen(CEA)in different population,and to explore their significances in diagnosis of pancreatic cancer.Methods The serum levels of CA199,CA125 and CEA in 56 patients with pancreatic cancer (pancreatic cancer group ), 5 6 patients with benign pancreatic disease (benign pancreatic disease group ) and 56 healthy volunteers(normal control group)were detected,then the positive rates of CA199,CA125 and CEA and the sensitivities and specificities of CA199,CEA and CA125 for the diagnosis of pancreatic cancer were compared among three groups.Results The levels of serum CA199,CA125 and CEA of the patients in pancreatic cancer group were significantly higher than those in benign pancreatic disease group and normal control group (P<0.05). The positive rates of CA199,CEA and CA125 in pancreatic cancer group were 87.50 %,71.42%,and 60.71%, respectively. The positive rates of the three tumor markers of the patients in pancreatic cancer group were obviously higher than those in other two groups (P<0.05).The sensitivity of CA199(87.50%)was the highest,while the specificity of CEA (9 1.9 6%)was the highest. The method of parallel combined testing increased the diagnostic sensitivity to 95.12% and negative predictive value to 90.47%,but its specificity and the positive predictive value were decreased. Serial combined examination increased the diagnostic specificity to 92.86% and the positive predictive value to 89.19%,but its sensitivity and negative predictive value were decreased.Conclusion Combined detection of serum levels of CA199,CA125 and CEA has higher sensitivity and specificity than single-marker detection in the diagnosis of pancreatic cancer.

Objective To investigate health care professionals′attitude of patient involvement in patient safety and analyze its influencing factors. Methods A convenience sample of 946 health care professionals recruited from twelve hospitals in Shanghai was employed for a cross-sectional survey. Results The general attitudes of patient involvement in patient safety scored 3.86 ±0.56, the perception of the content of patient involvement scored 3.97±0.54, the average score of caring involvement (4.12±0.59) was larger than the average score of decisive involvement (3.88±0.58), the average score of complaining involvement was the least (3.39±0.81), the difference had statistical significance (F=583.797, P<0.01). The regression analysis showed that identity, professional title, hospital grade influenced health care professionals′general attitude of patient involvement in patient safety and that identity, hospital grade influenced health care professionals′attitude of the content of patient involvement. Conclusions In health care professionals′opinion, patient involvement in patient safety has both advantages and disadvantages. They preferred to caring involvement, followed by decisive involvement, then complaining involvement. So, hospital managers should view patient involvement in patient safety discriminately and construct rational involvement model ofpatients willing, medical support, promote interaction and harmony build.

Objective To explore the effects of family and telephone follow-up on quality of life of the patients with tota1 hip arthroplasty(THA).Methods One hundred and twelve patients with THA were randomly divided into experiment group and control group with 56 cases in each group.The experiment group was treated with rehabilitative instruction by family and telephone follow-up.The control group was treated with outpatient follow-up after leaving the hospital.The living quality in both groups were compared by the medical outcomes study 36-item short-form health survey(SF-36 QOL)one year after leaving the hospital.Results One-year after leaving the hospital,the total score and demension score of experiment group were better than those of the control group.There was significant difference between the control group and in experiment group(P<0.05).Conclusion Family and telephone follow-up is an effective implementation for THA patients to accomplish physiological functional recovery and promote the quality of life.