Objective To investigate Anti-aging function of Placenta freeze-dried powder on mice. Method 2 month old female-KM mice were divided into five groups:normal control group, aging model group, positive control group, placenta freeze-dried powder low dose group and high dose group. Except normal control group,the rest of groups were treated with method of subcutaneous continuous injection of D-galactose for 42 days in order to establish mice aging model. Meanwhile, the corresponding drugs, via intragastric administration, were ingested by different treated groups and observe the change of immunity and physiological regulation related in mice. Results Compared with aging model group, thymus index and spleen index in placenta freeze-dried powder low dose group and high dose group increased obviously (P<0.05), content of MDA in brain decreased significantly (P<0.05), proportion of CD 4 and CD 8 lymphocyte in total lymphocyte, female hormone(P and E 2), IgG and haemopoietic factor in peripheral blood increased remarkably. Conclusion Placenta freeze-dried powder could slow the aging process on mice via immunity enhancement and improving physical regulation.

OBJECTIVETo investigate changes in gene expression that occur upon treatment with human umbilical cord mesenchymal stem cells (UC-MSCs) for hepatic cirrhosis using a rat model system.

METHODSHepatic cirrhosis was induced in Sprague-Dawley rats by subcutaneous injection of carbon tetrachloride and oral administration of alcohol.UC-MSCs were isolated from human umbilical cord and the cells' immunophenotype and differentiation towards osteogenic and adipogenic lineages were confirmed.The UC-MSC sample or vehicle alone (phosphate buffered saline, PBS) was transplanted by intravenous injection.Histopathological staining and serological testing were used to compare the liver morphology and function among the different groups.The gene expression in the PBS group and UC-MSC group were detected by gene microarray and differences between the groups were statistically analyzed by t-test.

RESULTSTransplantation of the UC-MSCs improved liver function in the hepatic cirrhosis rats.Comparison of the gene expression profiles of the PBS group and the UC-MSC group showed that the latter had up-regulation of the genes related to the complement and coagulation cascades and down-regulation of the genes related to cell proliferation, cell cycle, and collagen synthesis.

CONCLUSIONUC-MSC therapy might improve liver function in cirrhosis by increasing the expression of genes related to the complement and coagulation cascades and by decreasing genes involved in cell proliferation and collagen deposition.

Animals ; Carbon Tetrachloride ; Cell Differentiation ; Cell Proliferation ; Down-Regulation ; Gene Expression Profiling ; Humans ; Liver Cirrhosis ; Mesenchymal Stromal Cells ; Rats ; Rats, Sprague-Dawley ; Transcriptome ; Umbilical Cord ; Up-Regulation

Objective To assess the retention of ²⁴¹Am in the whole-body bone of worker by measuring the retention of ²⁴¹Am in the skull. Methods A whole-body counter with high-purity germanium detector was used to measure the 59.5 keV full-energy peak count of ²⁴¹Am in the skull. The efficiency of the measurement was calibrated by the digital skull phantom combined with the geometric model of the detector. The retention of ²⁴¹Am in the worker’s skull was calculated. Results The proportion of skull dry weight relative to the whole-body bone dry weight was used as the coefficient to calculate the ²⁴¹Am deposition in the whole-body bone from the measured activity of skull ²⁴¹Am. Conclusion With the retention of ²⁴¹Am in the skull and the proportion of skull dry weight, the retention of ²⁴¹Am in the whole-body bone of the worker was calculated to be about 806 Bq.

Objective Based on the lanthanum bromide scintillator detector, the calculation method of G(E) function was developed to measure the air absorbed dose rate. Methods Firstly, the gamma energy spectrumof the lanthanum bromide detector were simulated and the response functions with different energies were determined with Monte Carlo simulation method. Then, the G(E) function was calculated by the least square method. Finally, the air absorbed dose rate measured by the lanthanum bromide detector using G(E) function conversion method was compared with the theoretical calculation value based on the point source experiments. Results The experimental verification results showed that the relative deviation between thecalculated value with G(E) function and the theoretical calculation value wasmostly controlled within ± 6%, which verified the accuracy of G(E) function. Conclusion The results showed that the method of G(E) function could be applied to calculate the gamma radiation dose rate based on the in-situ the gamma spectrum with LaBr₃ detector.