Objective:To estabhish rat model of epilepsy induced by the Lithium-repeated low-dose pilocarpine.Methods:A totol of 173SD male rats were divided into the normal control group,seizure-induced group and Chloral Hydrate group randomly.Lithium chloride was injected at a dose of 3 mEq/kg intraperitoneally(ip) 19h prior to the administration of pilocarpine.Rats were injected with pilocarpine,10mg/kg ip,every 30min,and this injection was repeated for 2~4 times.We then determined the rat activity,EEG,the percentage of rats exhibiting status epilepticus(SE),the mortality rate,the proportion of rats developing spontaneous seizures,the latency for the first spontaneous motor seizure,and the frequency of the spontaneous recurrent seizures.Results:The lithium-repeated low-dose pilocarpine resulted in SE after 1~4 injections;the average period between seizure and injection was 16min and the success rate was 73%.The mean dose of pilocarpine needed to induce SE was 25.9mg/kg.When SE was interrupted after 90min,mortality rate was below 10%,Spontaneous seizure occurred 2.4~5.7 times a day averagely,and average latency to the first spontaneous seizure was approximately 40days.All seizure incidence was over level Ⅲ and most were level Ⅳ/Ⅴ;EEG was similar to that of human temporal epilepsy;seizure of rats in control group was level 0.Conclusion:Lithium-repeated low-dose pilocarpine induced rats model of epilepsy is characterized by easy building,with high successful rate and lower mortality of the animal.This model has mimic features of human status epilepticus and temporal epilepsy in seizure behavior and electroencephalographic findings.

0.05),while compared with I/R group, the decrease of positive cells counting in the CC group was significance(P

Objective:To explore the vascular protective effects and mechanism of fasudil in rats after experimental subarachnoid hemorrhage.Methods:Forty-eight rats were divided into sham-operated control group,subarachnoid hemorrhage group and treatment group.Model induced by autologous blood injection into cisterna magna,and rats were killed on day 1,day 3,day 7 and day 14 after subarachnoid hemorrhage.Morphological change of basilar artery was observed by light microscopy,diameter and wall thickness of basilar artery were measured respectively.eNOS expression in basilar artery was detected by immunohistochemical SP method.Results:Vasospasm of basilar artery initiated on day 1,reached its peak on day 3,and mitigated on day 7 after SAH.eNOS expression decreased at each time point in SAH group.Compare with SAH group,diameter and wall thickness of basilar artery were significantly reduced on day 3 and day 7 in treatment group(P

Objective To investigate the correlation between mechanical ventilation (MV) initiation time during cardiopulmonary resuscitation (CPR) and the prognosis of patients with in-hospital cardiac arrest (IHCA) in emergency . Methods Retrospective analysis of clinical records of patients with IHCA in emergency from January 2011 to April 2016 treated in Zhangye People's Hospital of Hexi University was performed. Patients with restoration of spontaneous circulation (ROSC) and were on MV with aged over 18 years were divided into early treated group (≤ 10 minutes) and later treated group (> 10 minutes) according to the initiation time of MV. Corneal reflex, pupillary reflex, pain-avoidance responses and motor response 24 hours after CPR, neurological function and cure rate of the two groups were analyzed. Results 210 patients were selected into our study including 130 males and 80 females (mean age: 60.24±13.17 years). There were no significant differences in gender, age, type of heart rate and etiological factor of cardiac arrest (CA) between the MV early stage group (124 cases) and the MV late stage group (86 cases). The restoration of corneal reflex, pupillary reflex, pain-avoidance responses, motor response and achievement ratio of CPR in early group were higher than those of later group (respectively, 59.68% vs. 31.40%, 59.68% vs. 31.40%, 54.84% vs. 24.42%, 54.84% vs. 24.42%, 70.16% vs. 51.16%, all P < 0.01); The D-dimer levels in the early group patients were significantly lower than those in the later group (μg/L: 478.39±57.21 vs. 510.05±62.83, P < 0.01). However, no statistical difference was observed between the two groups with respect to pH (7.24±0.72 vs.7.13±0.67, P > 0.05); The average hospitalized day of the early group was significantly shorter than that of the later group (days: 24.15±3.04 vs. 30.28±4.17, P < 0.01); Besides, the early group showed a higher survival rate at discharge and had more cases with neurologic level of grade 1-2 than those of the later group (Respectively, 41.94% vs. 26.74%, P < 0.05; 44.35% vs. 15.12%, P < 0.01). Conclusion Initiation MV on IHCA patients in the early stage of CPR (≤ 10 minutes) could help improve the hypoxic condition and prognosis of neurological function, and increase the achievement ratio of CPR.

Objective:To evaluate online bidding of equipments in research institutions.Methods: To analysis and practice the new online bidding procedure of central government procurement center.Results: Through the practice, discussed problems, proposed the measures of application. Conclusion:The online bidding’s advantages are obvious. This way will improve the quality of equipments and promote the procurement efficiency.

Objective To investigate the effects of autologous platelet-rich plasma in the treatment of refractory wound.Methods Fifteen cases with refactory wound ( 1 1 males and 4 females) involving six cases with diabetic ulcer,five with post-surgical ulcer wounds and four with pressure sore were enrolled into the study.The autologous platelet-rich gel was applied to the wounds after debridement and dressing change was done every week.The results of wound bacterial culture were regularly detected and the growth of granulation was observed.Skin graft or flap graft surgery was performed to close the wound when the wound condition was permitted.Results Of all,three patients had spontaneous healing after the change of dressing,four got healing after flap graft and eight attained healing after skin graft.There was no recurrence or sinus after treatment.Conclusion Autologous platelet-rich gel accelerates wound healing,improves wound condition,and raises success rate of surgical closure of the wound.

Esophageal carcinoma represents high incidence and mortality rate in China.Irinotecan (CPT-11) has shown promising activity in a number of gastrointestinal cancers,including esophageal cancer.This article reviews recent clinical trials of irinotecan-based chemotherapy and radiochemotherapy in esophageal carcinoma.

The determination of tumor markers is of great value for esophageal squamous cell carcinoma. This article reviews the application status of routine tumor markers and the progression on the role of tumor markers in early diagnosis, predicting chemotherapy or radiotherapy response, monitoring disease recurrence and evaluation of prognosis in esophageal squamous cell carcinoma.

Macrophages are major effecter cells of nonspecific immune response,the polarization of which plays a great role in inflammation,repairing and angiogenesis.According to functional phenotypes,macrophages can be polarized to classically activated type (M1),which could promote angiogenesis,and alternatively activated type (M2),which could inhibit angiogenesis.The proportion of M1/M2 could modulate the growth of choroidal neovascularization (CNV).Under the conditions of aging and injury within the retina,macrophages may polarize to M2,which could generate several proangiogenic factors,initiating and promoting the formation of angiogenesis and fibrous scar.Therefore,regulation of macrophage polarization is expected to inhibit angiogenesis and provide new insight for treatment of CNV.

Objective To preliminarily investigate the effect of high mobility group box 1 (HMGB1) in the pathogenesis of chronic obstructive pulmonary disease (COPD) by detecting the HMGB1 level in bronchoalveolar lavage fluid(BALF) of the COPD patients and analyzing the relation between HMGB1 and airway limitation .Methods The research subjects were divided into the COPD group (stable COPD ,20 cases) and control group[20 cases of upper airway cough syndrome(UACS)] .All subjects were performed the bronchoscopic examination as well as bronchoalveolar lavage .The total cell counting and percentage of neutrophilic granulocytes in BLAF were detected and compared between the two groups .The HMGB1 ,IL‐1βand TNF‐αlevels in BLAF were detected by using ELISA and compared between the two groups .The relation between HMGB1 with IL‐1βand TNF‐αand the rela‐tion between HMGB1 with pulmonary function(FEV1% predicted value) in the COPD group was analyzed .Results The total cell counting and percentage of neutrophilic granulocytes of BLAF in the COPD group were higher than those in the control group (P<0 .01) .The levels of HMGB1 ,IL‐1β,TNF‐αof BALF in the COPD group were higher than those in the control group(P<0 .01 , P<0 .05 ,P<0 .01) .In the COPD group ,the HMGB1 level in BALF was positively correlated with the IL‐1βand TNF‐αlevels(r=0 .79 ,P< 0 .01 ;r= 0 .48 ,P< 0 .05) ,while negatively related with the pulmonary function value(FEV1% predicted value ,r=-0 .70 ,P<0 .01) .Conclusion HMGB1 participates in and promotes the occurrence and development of COPD airway inflamma‐tion and the HMGB1 level in BALF is correlated with the severity of COPD .