OBJECTIVE: To evaluate the efficacy and safety of recombinant human Ad-p53 injection (rAd-p53) combined with cisplatin in the treatment of malignant pleural effusions of non-small-cell lung cancer (NSCLC). METHODS: 46 patients with malignant pleural effusions of NSCLC were randomly divided into groupⅠ, Ⅱand Ⅲ. The groups were given cisplatin, rAd-p53 and combination medication, respectively once a week. The efficacy and quality of life were evaluated after 4 weeks. RESULTS: There were significant difference in among three groups in effective rate and improvement rate of life quality (P0.05). CONCLUSION: rAd-p53 combined with cisplatin has sound effect on malignant pleural effusions of NSCLC without aggravating effect on the toxicity of chemotherapy, which is worth of spreading in the clinic.

Objective To investigate the effect of postoperative analgesia with oxycodone on T cell function after operative of cesarean section with chronic hepatitis B.Methods Sixty cesarean sec-tion women with chronic hepatitis B undergoing CS,aged 22-35,were randomly divided into two groups:oxycodone group (group O)and morphine group (group M).The changes of immune cells (Th1,Th2)and liver function were recorded after the analgesia (immediate,postoperative 24 h,48 h,72 h).The total number of pressing analgesia pump and the cumulative amount of PCA were re-corded.Results The Th1 of group O was higher than that of group M at 24 h,48 h after operation (P <0.05),while there was no significant difference of Th1 and Th2 in group M.The total patient-controlled pressing times and accumulated amount of PCA of group O were significantly lower than those in group M (P <0.05).There was no significant difference in the incidence of adverse reactions between the two groups.Conclusion Oxycodone can activate T cell function in postoperative analge-sia,while morphine causes the inhibition of Th1 cells.

Objectives To analysis the expression difference of eIF4E in bone marrow between the acute myeloid leukemia (AML) and the non-tumor patient,before and after induction therapy,and the different subtypes of AML,and to explore the relation between eIF4E and other molecular biology abnormalities in AML.Methods The bone marrow specimens of newly diagnosed AML and non-tumor control patients were collected.The expression level of eIF4E was detected by RT-PCR.Results The positive rate of eIF4E was 65.2 ％ (101/155) in AML.eIF4E turned to negative in 12 patients (17.6 ％) who got complete remission after induction therapy.eIF4E was negatively expressed in bone marrow of the nontumor control patients.The positive rates of eIF4E were 75.0 ％ (15/20) and 80.8 ％ (21/26) in M4 and M5 type AML,respectively,and was 59.6 ％ (65/109) in other subtype AML (P ＞ 0.05).FLT3/ITD gene mutation was found in 26 cases of newly diagnosed AML.The eIF4E expression and FLT3-ITD gene mutation were independent each other (P ＞ 0.05).Conclusions eIF4E is positive in most of the newly diagnosed AML and turns to negative in part of AML achieving complete remission.The expression of eIF4E is no difference among different subtype of AMLs.eIF4E and FLT3-ITD gene mutation are independent each other.

Objective:To investigate the changes of plasma moderate molecule substance (MMS)level in pediatric patients with congenital heart disease during open heart surgery. Method: Siteen cases were studied. Blood samples were taken before anesthesia, after tracheal intubtion, after sternotomy. and rewarming during cardiopulmonary bypass, to measure the plasma MMS levels with ultraviolet absorption spectrophotometry. Result: As compared with that before anesthesia, the MMS levels after tracheal intubtion,sternotomy,and rewarming during cardiopulmonary bypass were significantly elevated(P

Objective To explore the biological characteristics of mucinous adenocarcinoma deriving from chronic anal fistula and its diagnosis and treatment. Method A retrospective study was made to analyze clinical and pathological features of 4 cases of chronic anal fistula from which mucinous adenocarcinoma developed in our hospital from 1986 to 1997. Results There were three males and one female averaging 50 years old. All patients had chronic history of perianus abscess or anal fistula. This rare mucinous adenocarcinoma originated from the anal fistula and the associated anal glands. The canceration of anal fistula was due to chronic inflammation and scarring. The definitive diagnosis of the carcinoma depends on biopsy of the fistula and the related tumor. Metastasis to groin lymph nodes was found in two patients. Three cases underwent Miles operation with chemotherapy; two of the three patients also had resection of groin lymph nodes. Three patients survived more than five years. One patient treated only with chemotherapy died three months later. [WT5”HZ] Conclusion [WT5”BZ] Mucinous adenocarcinoma following chronic anal fistula is less malignant and has better prognosis compared to primary rectal and anal mucinous adenocarcinoma.

Objective To assess the risk factors of adrenal incidentalomas during follow up. Methods In 43 patients with adrenal incidentalomas, the significant risk factors were screened by univariate analysis, and the multivariate analysis was evaluated by logistic regression. Results After an average of (4.0?1.2)years of follow up,4 out of in 21 patients with adrenalectomy were verified to be malignant. By means of multivariate analysis, tumor size ( P =0.018)and abnormal level of hormonal or its metabolic products ( P =0.039)were the risk factors for adrenal incidentalomas and adrenalectomy should be considered when indicated. Conclusions The gains and losses of open surgery for adrenal incidentalomas should be weighed up.If the evaluation of risk factors reveals a possibility of malignancy,adrenalectomy should be undertaken.

0.05).[Conclusion]Both SF and NH/C have good biocompability with BMMSCs and could be used as an ideal scaffold material in tissue engineering.

Objective: To establish an on-line column switching liquid chromatography for quantitative determination of vitamin D3 in poultry eggs and their products. Methods: Antioxidant-protected samples were saponified, extracted with a mixture of ethyl acetate-n-hexane mixture (6︰4, v/v) and subjected for determination using two-dimensional liquid chromatography. One-dimensional chromatography was performed with the UniChiral® OD-5H column (4.6 mm × 100 mm, 5 μm), acetonitrile: methanol (75︰25, v/v) and water as a mobile phase for gradient elution at a flow rate of 1.00 mL/min to complete vitamin D purification, and two-dimensional chromatography was performed with the Agilent Eclipse PAH column (2.1 mm × 100 mm, 3.5 μm, column temperature of 35 ℃), detection wavelength of 264 nm for separation and determination of vitamin D3 and internal standard vitamin D2 with the internal standard method. Results: Vitamin D3 had a good linear relationship within the range of 2.5 to 100 ng/mL, with r2 of >0.999. The mean spiked recovery rate of vitamin D3 was 101.23% to 102.08% in egg yolk powder, with relative standard deviation (RSD) of 3.91% to 5.85%, detection limit of 1.5 μg/100 g, and quantitative limit of 4.9 μg/100 g, and the limits of detection and quantitation of vitamin D3 were 0.15 μg/100 g and 0.49 μg/100 g in whole egg liquids. Conclusions The method is simple, rapid, highly accurate, sensitive and reproducible, which is suitable for rapid and quantitative determination of vitamin D3 in poultry eggs and their products.

0.05)respectively.Conclusions:Treatment of condylar process fracture should be based on the type of the fracture.

BACKGROUND:The mesenchymal stem calls (MSCs) have multiple-direction differentiation potential.How to induce human mesenchymal stem cells (hMSCs) into chondrogenic in vitro is an advanced researching direction.OBJECTIVE: Experimental study on chondrogenic differentiation of mesenchymal stem cells from human bone marrow in study culture medium so as to observe the characteristic of the cells in the continuous progressive process,such as culture of the primary generation and passage culture.DESIGN: A single sample and open study.SETTING : Department of Orthopaedics, Children's Hospital Affiliated to Soochow University.MATERIALS:A total of 5 children with single bone cyst were selected from Children's Hospital Affiliated to Soochow University from August 2002 to September 2003,including 3 boys and 2 girls aged from 3 to 12 years.All of them donated 5 Ml bone marrow, which was added with 1 Ml (20 U/Ml) heparinization, from which MSCs was obtained. Main reagents were detailed as follows: F-12 (Gibco, USA), fetal calf serum (Sijiqing Bioengineering Company, Hangzhou), trypsin (Sigma, USA), transforming growth factor-β1 (TGF-β1) (Sigma, USA) and vitamin C (the Third Medical Company,Nanjing; batch number: 021017).METHODS:① Standard culture medium:0.1 volume fraction of fetal calf serum,100 U/Ml penicillin,100 U/Ml streptomycin, 5.8 g/L Hepes, 2 g/L NaHCO3 and 0.3 g/L glutamine were added into F-12 culture medium (Ph 7.2-7.4). ②Study culture medium: 50 μg/L vitamin C and 1 μg/L TGF-β1 were added into standard culture medium (Ph 7.2-7.4). ③Cell culture of the primary generation: 12 Ml Hanks which contained 1 Ml of heparinization (20 μ/Ml) and 5 Ml bone marrow was centrifugated. Then the cells which had nucleolus were rinsed. Then cells were cultured in a 50 Ml plastic culture bottle at the density of 3×109 L-1 and were cultured in 6-well culture plate at the density of 1×109 L-1. One bottle and 3 well was used study culture medium,the other bottle and 3 well was used standard culture medium.The cells were cultured in incubator containing 0.05 volume fraction of CO2 and saturated tumidity at 37℃. The medium was changed every 48 hours. The cells were observed under inverted microscope. ④ Passage culture: When the primary generation cells had been cultured with standard culture medium for 10-14 days, we would find that the cells covered 80％ of the bottle bottom. Then 2.5 g/L of trypsin containing 0.2 g/L of EDTA was used to digestion and passage. The third generation cells were separated at the ratio of 1:1, and were cultured in two bottles. One bottle used standard culture medium, the other used study culture medium. At the same time, the cells in 6-well plate which contains coverslip were deal with in the same way. 3-wells used standard culture medium, and the others used study culture medium. Culture time was7 days.The cells were observed under inverted microscope.⑤ Index detection:Alcian blue staining was used to show the expression of the glycosaminoglycan of the primary generation and the third generation. Type- Ⅱ collagen immunohistochemical staining was used to show the express of the type- Ⅱ collagen of the primary generation and thethird generation.MAIN OUTCOME MEASUREMENTS:① Morphological observation; ② secretion of glycosaminoglycan; ③ expression of type- Ⅱ collagen.RESULTS: ① Observation under inverted microscope: Most of the hMSCs in primary culture were spindle; a few of them were wide, flat and polygon. After passage, the cells were found in a uniform spindle shape. The cells, which were induced in TGF-β1 and vitamin C, became round or oval shape. ② The positive rate of primary generation was 82.4％, the 3rd generation which were cultured in study culture medium was 76.3％ (x2 =1.14,P＞ 0.05), and the 6th generation was 68.5％(x2 =5.22, P ＜ 0.05). The cells, which were culturedinstandardculturemedium, were negative. ③ The 3rd generation and 6th generation cells which were cultured in study culture medium, were found that brown-yellow granules distributed in cytoplast. It was the positive reaction. The cells, which were cultured in standard culture medium, were negative.CONCLUSION:MSCs derived from human bone marrow may be inducedinto hondrocytes under study culture medium which contained TGF-β1 and vitamin C in vitro.The express of the glycosaminoglycan and type Ⅱ collagen show the characteristic of chondrocyte.