Objective To compare the effect of dynamic hip screw(DHS) locked intramedullary nail and the proximal femur with (PFN) in the treatment of intertrochanteric fracture.Methods 88 patients with femoral intertrochanteric fracture were selected.According to the patients' intention and economic status,they were divided into two groups.43 cases in group A were treated by DHS internal fixation,45 cases in group B were treated by PFN internal fixation.The clinical efficacy,the healing time and complications were observed.Results The excellent and good rate in group A and group B were 95.35％,97.78％,there was no significant difference between the two groups (x2 =0.394,P ＞ 0.05).In group B,the healing time,intraoperative bleeding volume and the incidence rate of complications were significantly lower than those in A group (t =3.824,4.017,x2 =4.552,P ＜ 0.05).Conclusion DHS and PFN in treatment of femoral intertrochanteric fractures can achieve better efficacy,however,PFN can significantly reduce postoperative complications,and shorter healing time,which is worth to be widely applied in clinical.

Objective To identify differentially expressed proteins in keloid tissue and to explore the proteomic characteristics of keloid by utilizing capillary high-performance liquid chromatography (LC) and tandem mass spectrometry (MS).Methods Tissue specimens were obtained from the lesions of 5 patients with non-familial keloid and normal skin of 5 human controls.After extraction and in-solution digestion of proteins,the resultant peptides were separated and identified by LC-MS/MS.Label-free detection and quantitation of peptides were carried out by using the DeCyder MSTM software.Results A total of 570differentially expressed peptides were identified between non-familial keloid and normal tissue (all P ＜ 0.05),which correspond to 1221 proteins.A difference greater than 1.5 folds was observed in the expressions of 293 proteins,including 124 up-regulated and 169 down-regulated proteins in non-familial keloid tissue.Conclusion Differences exist in the expression of proteins between non-familial keloid and normal skin tissue.

Objective To discuss the efficacy and safety of different second-line chemotherapies in small cell lung cancer chemotherapy.Methods 170 patients with small cell lung cancer who were failure to first -line treatment were chosen,they were randomly divided into observation group and control group.The control group was given paclitaxel plus cisplatin second-line chemotherapy,the observation group used the irinotecan joint cisplatin second-line chemotherapy.The effect of the two groups was recorded.Results The effective rate of the observation group was 45.88%,which of the control group was 25.88%,the difference between the two groups was statistically significant (χ2 =7.389,P<0.05).The median survival time,total survival time,median disease-free survival time, Karnofsky score,pain score of the observation group were (10.32 ±2.41)months,(7.89 ±1.88)months,(7.54 ± 1.78)months,(83.23 ±6.89)points,(2.37 ±1.07)points,which of the control group were (7.45 ±1.03)months, (3.16 ±0.79)months,(3.89 ±0.68)months,(69.97 ±2.33)points,(4.81 ±2.13)points,the differences between the two groups were statistically significant (t =10.095,21.384,17.660,21.384,17.660,all P<0.05).In the observation group,nausea and vomiting occurred in 5 cases,thrombocytopenia in 3 cases,anemia in 1 case,leukopenia in 7 cases,hypodynamia in 12 cases.In the control group,nausea and vomiting occurred in 16 cases,thrombocytopenia in 11 cases,6 cases of anemia,leukopenia in 18 patients,hypodynamia in 26 cases,the differences between the two groups were statistically significant(χ2 =6.574,4.981,4.722,4.981,4.722,all P <0.05).Conclusion The second-line chemotherapy regimens with irinotecan used in small cell lung cancer patients can improve the treatment effect,adverse reaction mild,prolong patients'survival,it is worth popularizing in clinical.

Objective To investigate the variations of hepatic artery and its extrahepatic arteries on hepatic arteriogram and to provide benefit for transhepatic arterical chemoemblization.Methods The hepatic arteriograms of 200 cases with unresectable hepatic malignant tumor before interventional therapy were analysed.Two interventional radiologists in common reviewed the incidences of various types according to Michels' classification,the absence of proper hepatic artery,and the variations of extrahepatic arteries originating from hepatic artery.Results The most common hepatic artery variation was Michels type Ⅲ(n=17,8.5%),followed by type Ⅱ(n=10,5.0%) and Ⅴ(n=9,4.5%).Proper hepatic absence was found in 25 cases and appeared as 5 subtypes.5 kinds of extrahepatic arteries were found.The most common extrahepatic artery was the right gastric artery(n=156,78.0%),followed by cystic artery(n=126,63.0%),accessory left gastric artery(n=19,9.5%),the hepatic falciform artery(n=5,2.5%),and accessory left inferior phrenic artery(n=4,2.0%).Conclusion There are some other variations of hepatic artery beside Michels' classification,and there are many variations of extrahepatic arteries originating from hepatic artery,it is important to assure interventional therapy effect for hepatic cancer and prevent complication.

Objective To evaluate the method of flow cytometric analysis of T lymphocyte subsets in bodyfluid of the pa-tients. Methods In ovder to get T lymphocytes by 3-colour staining method utilizing SSC/CD3Percp antibody,47 bronchoalveolar lavage fluid and 50 were tested by flow cytometery. Results 1)The percentage of Th is (67.98±7.41) % ,Ts(27.36±6.41) % and Th/Ts(2.60±0.64)in BALF with lung cancer,and the percentage of Th is (37.61±4.8)% ,Ts(67.97±4.20)% ,and Th/Ts(0. 47±0.10)in BALF with lung cancer respectively, and the Th cells in BALF with lung cancer were lower than those in the benign pulmonary disorder(P<0.05), and the Th/Ts ratio was significantly lower while the Ts cells were significantly higher in BALF of lung cancer(P<0.01), comparied with the benign pulmonary disorder; 2)The percentage of Th is (35.76±7.71) % and Ts(64.31 ±7.37) % and Th/Ts(0. 57 ± 0. 18) in BALF with lung cancer, and the percentage of Th is(49.27 ± 12.88) %, Ts(44.54 ± 9. 56) %,and Th/Ts is (1.13 ± 0.29) in BALF with benign pulmonary disorder. The Th cells in BALF with lung cancer was lower than those in the benign pulmonary disorder(P<0.05) ,the Th/Ts ratio were significantly lower and the Ts cells were significantly higher in BALF of lung cancer(P<0.01) comparied with the benign pulmonary disorder;3)The percentage of This(35.76±7. 71) %,Tsis(64.31 ± 7.37) % and Th/Ts are(0.57 ± 0. 18) in BALF with lung cancer, and the percentage of Th is (49.27 ± 12. 88) %, Ts is (44.54 ± 9.56) %, and Th/Ts is(1.13 ± 0.29) in BALF with benign pulmonary disorder. Respectively, the Th cells in BALF with lung cancer were lower than those in the benign pulmonary disorder(P<0.05), the Th/Ts ratio were significantly low-er and the Ts cells were significantly higher in BALF of lung cancer(P<0.01)comparied with the benign pulmonary disorder. Con-clusion The immunity function in the patients with lung cancer was significantly decreased. Flow cytometric approach is valuable in analyzing T lymphocyte subsets in BALF.

Good clinical results were shown in nasopharyngeal carcinoma (NPC) patients received radiotherapy. The patients with long term life partially had radiation induced injuries that severely affect the quality of life (QOL). The study on QOL of those patients is important. Our article reviewed the effects of NPC QOL by treatment options, health education, rehabilitation therapy, traditional chinese medicine and qigong. The current measurement scale of NPC QOL in China was also discussed.

OBJECTIVE:To observe the effect of metformin on related indicators of obese patients with type 2 diabetes. METHODS:136 obese patients with type 2 diabetes were randomly divided into control group and observation group. All patients were given conventional examination,conventional education about diabetes,diet control and stressed physical exercise. Based on it,control group was given 80 mg Metformin tablet(Ⅱ),once a day,and monitored blood glucose levels,the dosage was adjusted based on fasting blood glucose levels to ensure blood glucose in 3.9-6.1 mmol/L;observation group was given 0.5 g Metformin en-teric-coated tablet,twice a day. The 2 groups were compared after 12 weeks treatment. The glycated hemoglobin (HbA1c),body mass index (BMI),resistin,insulin-like growth factor-1(IGF-1),inflammatory cytokines [interleukin-1β(IL-1β),interleukin-6 (IL-6),tumor necrosis factor-α(TNF-α),high-sensitivity C-reactive protein(hs-CRP)] levels in 2 groups before and after tveat-ment were observed,and the incidence of adverse reactions in 2 groups was recorded. RESULTS:After treatment,resistin,IGF-1, IL-6 and hs-CRP levels in 2 groups were significantly lower than before,and observation group was lower than control group;the differences were statistically significant(P<0.05);BMI,IL-1β and TNF-α levels in obser vation group were significantly higher than before and control group,the differences were statistically significant(P<0.05);HbA1c level in 2 groups was significiantly lower than before,the difference was statistically significant(P<0.05),but there was no significant difference between 2 groups(P>0.05);BMI level in observation group was lower than before and control group,and there was no significant difference in the inci-dence of adverse reactions between 2 groups(P>0.05). CONCLUSIONS:Metformin can effectively improve insulin resistance in obese patients with type 2 diabetes and inhibit the inflammatory reaction.

Radish phospholipid hydroperoxide glutathione peroxidase (RsPHGPx) was identified as a mitochondrion-targeting PHGPx in previous work. To determine its cleavage site of the targeting peptide, the immunoaffinity chromatography (IAC) purification approach was carried out to isolate the native RsPHGPx protein.Polyclonal antibodies directed against recombinant RsPHGPx were raised in rabbit. Monospecific anti-RsPHGPx antibodies were isolated by means of affinity chromatography using the recombinant RsPHGPx as affinity ligand, and employed in assembling an IAC column. A single-step, highly specific and easy-to-use protocol was developed for purification of the active RsPHGPx protein through the assembled IAC column. Using this approach, a specific protein of the expected molecular size was obtained from the mitochondrial fraction of radish seedlings. Western blot analysis showed that it could be specifically recognized by anti-RsPHGPx antibodies, and an enzyme activity assay indicated that it exhibited significant PHGPx activity, suggesting that the purified protein should be the desired native RsPHGPx. These results will lead to clarification of the targeting peptide and the active mature protein of RsPHGPx and will be helpful to further probe the intracellular localization mechanism and biological fun ction of this plant PHGPx.

A novel radish RsPHGPx cDNA, which encodes a functional phospholipid hydroperoxide glutathioneperoxidase (PHGPx) protein, was identified in the previous work. In the study genomic organization and the upstream regulatory sequence analysis of this gene was presented. Southern blot analysis showed that RsPHGPx gene existed in radish genome in manner of single copy. Moreover, a 3.3 kb genomic DNA fragment of RsPHGPx gene was isolated by combination of common PCR and genome-walking method. Sequence analysis on this genomic fragment demonstrated that RsPHGPx gene consists of seven exons separated by six introns, and suggested that a short 5'-flanking sequence immediately before the exon 1 should be the putative RsPHGPx promoter region, which is proceeded by the upstream neighboring biotin synthase gene. Cis-acting elements search showed that the putative promoter contains elements responsive to hormones (eg. E-Box and W-Box), abiotic stresses (eg. MYB and MYC binding sites), and light (Box Ⅱ and Ⅰ-Box), etc. Northern blot analysis indicated that the expression of RsPHGPx was subjected to up-regulation of chilling and down-regulation of ABA and successive illumination (in etiolated seedlings), implying the regulatory roles of some predicted elements. However the up-regulation effect of herbicide paraquat, which can induce oxidative stress, suggested the presence of some unknown elements in the promoter region. This is the first report on gene structure and upstream regulatory sequence analysis in reported plant PHGPx genes, which will be a prerequisite to understand regulatory mechanism of PHGPx gene expression in plants.

Objective: Our aim was to evaluate the biocompability of porcelain compounded with nanostructured material. Methods：The proliferation of the NIH3T3 cells were investigated using a tetrazolium-based colorimetric assay(MTT assay).Maceration extract of four tested materials were added into culture plate .The samples were tested with ultraviolet spectrophotometer in 490 wavelength at 1，3，5，7 days after the addition of extract. Morphology of these cells was also observed with phase-contrast microscope. Results： There was no obvious difference among three kinds of prosthetic materials in the optical absorbance and the cells growing in the extract showed normal morphology. Conclusion: The toxicity graduation of the porcelain compounded with nanostructured material is 0. This material has a good biocompability and can be used clinically.