Since human imprinted genes in 11P15.5 was found,it had been proved that various human physiological abnormality associated with it.As one of the first identified imprinted non-coding RNA genes,more attention is paid to lncRNA H19 by many scholars not only because it's linked with Beckwith-Wiedemann syndrome (BWS) and Russell syndrome (SRS),but also H19 gene is the only imprinted gene belonging to long non-coding RNA related with a variety of tumors which can also produce microRNA675 and lncRNA 91H coming from H19 gene antisense-coding.This paper will review possible mechanisms of H19 gene in tumorigenesis process and its research status in Cancer therapy from four aspects:imprinting feature,single nucleotide polymorphisms,lncRNA character and its possible action signal axis in cancer.

At present, coronary stent has been extensively used in clinic. It can significantly improve acute obstruction complication and vascular restenosis following percutaneous transluminal coronary angioplasty. However, restenosis also takes place after stent implantation, in particular for complex pathological changes. It has been demonstrated that thrombus, inflammation, and smooth muscle hyperplasia are three important stages for in-stent restenosis, and vascular intima hyperplasia is the major mechanism. After retrieving related articles, this paper discussed biocompatibility and restenosis of metal stent and drug-eluting stent, analyzed influence of stent implantation on collateral circulation function and inflammatory reactions, and explored the application of biodegradable stent.

Objective To investigate the correlation of serum adiponectin (APN),C-reactive protein (CRP) levels and carotid atherosclerosis plaque in patients with acute cerebral infarction (ACI).Methods Sixty patients with ACI (ACI group) were diagnosed by head CT and MRI examination.And sixty cases of healthy check-up were as control group.The serum APN and CRP were examined by double antibody sandwich enzyme linked immunosorbent assay method,and carotid atherosclerosis was examined by ultrasound.Results The serum APN and CRP levels in ACI group were (4.36 ± 2.54),(8.93 ± 2.89)mg/L,and in control group were (8.43 ± 3.51),(1.12 ± 0.52) mg/L.The serum APN level in ACI group was significantly lower than that in control group,and the serum CRP level in ACI group was significantly higher than that in control group (P ＜ 0.05).In ACI group,the serum APN level after treatment was significantly increased compared with that before treatment,the serum CRP level after treatment was significantly decreased compared with that before treatment.The differences were (6.44 ± 2.31) and (4.10 ± 0.72) mg/L respectively.The rate of carotid atherosclerosis in ACI group was 78.33％ (47/60),and the rate of carotid atherosclerosis plaque was 71.67％(43/60).The stable plaque was in 13 cases and instability plaque was in 30 cases.The rate of carotid atherosclerosis in control group was 26.67％(16/60),and the rate of carotid atherosclerosis plaque was 13.33％(8/60).The stable plaque was in 6 cases and instability plaque was in 2 cases.The differences between the two groups had statistical significance (P ＜ 0.05).In ACI group,the serum APN and CRP levels in ACI without carotid atherosclerosis plaque were (6.89 ± 2.37) and (5.41 ± 1.38)mg/L,in ACI with carotid atherosclerosis plaquewere (3.28 ± 1.99) and (9.42 ±3.70) mg/L.Carotid atherosclerosis plaque and serum APN level showed negative correlation (r =-0.76,P ＜ 0.05),and serum CRP level was positively correlated (r =0.81,P ＜ 0.05).The serum APN and CRP levels in ACI with stable plaque were (4.32 ± 2.14) and (6.65 ± 2.69) mg/L,in ACI with instability plaque were (2.11 ± 1.35) and (10.11 ± 3.82) mg/L.Carotid artery plaque stability and serum APN level showed negative correlation (r =-0.69,P ＜ 0.05),and serum CRP level was positively correlated (r =0.77,P＜ 0.05).Conclusions The occurrence of ACI is closely related to carotid atherosclerotic plaque.The serum APN level in ACI patients is significantly lower than that in normal crowd,but serum CRP level in ACI patients is significantly higher than that in normal crowd.The rate of carotid atherosclerosis is significantly increased,most of which is instability plaque.Carotid atherosclerosis plaque and serum APN level has negative correlation,and serum CRP level is positively correlated.Carotid artery plaque stability and serum APN level has negative correlation,and serum CRP level is positively correlated.

Objective To characterize the clinical features of patients with high level of carbohydrate antigen (CA)724 in order to facilitate the recognition of this clinical manifestations.Methods Eight-two cases of hospitalized patients diagnosed with gout were selected and divided into 2 groups according to the level of CA724,and the clinical features and laboratory indexes were compared between the 2 groups.T test was used in the numerical variable data between groups,x2 test was used in the categorical variable data.Correlation analysis was tested using Spearman analysis.Results The incidence of gout stones in the group with high level of CA724 was 61％,which was significantly higher than that in the group of normal CA724 of 28％.There were statistically significant differences between the two groups (x2=8.894,P＜0.05).The incidence of kidney stones in the group with high level of CA724 was 65％,which was significantly higher than that in the group of normal CA724 of 22％.There were statistically significant differences between the two groups (x2=15.013,P＜0.05).But there were no statistically significant differences in age of onset,blood uric acid,erythrocyte sedimentation rate,and serum creatinine between the two groups (P＞0.05).Conclusion The significantly elevated level of CA724 canappeare in gout patients,But it isnotcorrelated with tumor,which should be paid attention by clinician.It helps to avoid unnecessary checks and reduce the psychological and financial burden of patients.

OBJECTIVE:To investigate the compatible stability of cefepime dihydrochloride mixed with total parenteral nutrient(TPN). METHODS:Cefepime dihydrochloride mixed with TPN at room temperature for 24 h. The appearances of the mixture were observed at 0,2,4,6,8,10,24 h and the change of pH value was evaluated. The contents of cefepime dihydrochloride were determined by HPLC. RESULTS:No change was observed in the contents of cefepime dihydrochloride in total parenteral nutrient within 24 h. There was no significant change in the appearance,color and pH value of nutrient. CONCLUSION:At room temperature cefepime dihydrochloride in TPN is stable within 24 h.

Skin toxicities are the most common adverse effects of epidermal growth factor receptor inhibitors(EGFRI).It can result in significant physical and psyehological discomfort,and even leads to interruption or dose modification,and consequently affect the curative effect. Nowadays this adverse effect has attracted lot of attention.It is proposed that clinical management and treatment measures for skin toxicities should be further studied to improve the patients life quality,and guarantee the continuity and best curative effect of the treatment.But more researches on the pathogenesis and its prognostic significance are still expected.

The diagnosis and treatment of age-related macular degeneration (AMD) is an international hotspot of eye research.Successful clinical applications of anti-VEGF drugs promoted both basic research and clinical practice of AMD.A number of countries and professional societies have established clinical guidelines for AMD management,including the epidemiology,risk factors,diagnosis,classification,and treatment process.These AMD guidelines are mostly based on recently published results of clinical trials,provided good model of evidence based medicine.It is urgent and necessary to have our own guideline which is suitable for Chinese patients.Reviewing and learning existed guidelines will help us to improve the clinical practice of AMD in China.

Objective To study the effects and advantages of the treatment for simple bone cysts of the long bones in children with AO titanium elastic nail and autologous bone marrow injection. Methods Eighteen patients with a simple bone cyst at humerus or femur were managed with AO titanium elastic in-tramedullary nail combined with autologous bone marrow injection. There were eleven boys and seven girls in total, with the mean age of 8.1 years, ranged from 6 to 12 years. Twelve cysts was located in the proximal end of humerus, five in the proximal end of femur and one in the middle part of the shaft of femur. Twelve cysts were active and six inactive according to Neer standard. Radiographic evaluation was performed three, six, twelve and eighteen months after operation during the 11 to 23 months follow-up by taking anteroposteri-or and lateral X-ray pictures. Then we measured residual radiolucent areas on X-ray pictures and did statistic research with group comparison. Results Six cysts (33.3%) healed completely, and twelve (66.7%) healed with residual radiolucent areas which were visible on X-ray pictures 3 to 6 months after operation. Residual radiolucent areas on X-ray pictures gradually reduced during the different phases and it has statistical signif-icance by group comparison. There was no recurrence or major complication except for one patient with mild irritation of skin caused by the end of the nail up to now. Conclusion The treatment for simple bone cysts of the long bones in children combined with AO titanium elastic nail and autologous bone marrow injection not only has satisfactory healing rate but also can provide early stability to the long bones, and accelerate the healing of cysts which allows early mobilization and thus obviates the long-term need for a plaster cast and decreases the prevalence of the most common complication: a pathological fracture.

Objective To explore the personnel framework strategy of multi-disciplinary treatment (MDT) for colorectal cancer. Methods Combined the characteristics of large public hospital, with recognized treatment pathway in international MDT for colorectal cancer and management of medical human resource, colorectal cancer MDT project team summarized a system of multi-disciplinary treatment-working for colorectal cancer of West China Hospital (MDT-CRC-WCH) by own management mechanism and subject feature. Results Based on the main four principles——whole, match, voluntary and interactive, MDT-CRC-WCH constructed reciprocation “concentric circle” with the team of directors, coordinators, colorectal surgeons, related professors, nursing and other assistants.Conclusion With construction of multi-layers, MDT for colorectal cancer is completing MDT personnel framework and suitable layers relation. And how to construct prolong active and innovative system of MDT human resource management needed more research.

Objective To explore the whole constructive conception and organization structure strategy of multi-disciplinary team (MDT) for colorectal cancer (CRC). Methods Combined the characteristics of large public hospital, with recognized treatment pathway in MDT for CRC and the way of medical project construction, MDT for CRC project team summarized a system of MDT for CRC of West China Hospital (MDT-CRC-WCH) by own characteristics and subject feature. Results MDT for CRC summarized the 5 basic characteristics about profession, classification, interaction, optimization and fast. The project has the core competencies: system new operation types for colorectal cancer and volunteer culture. By the matrix organization structure, MDT set the main departments: database team, follow-up team, nursing team and public team. Conclusion With effective MDT whole construction and suitable organization structure, MDT will develop in long time.