Objective: To investigate the clinical value and significance of B ultrasonography guiding percutaneous gallbladder catheterized drainage (PGCD)in elder patients with acute chole-cystitis. Methods: The clinical data of 34 elder patients with acute cholecystitis in our hospital who were treated with PGCD were analysed retrospectively, and comparing with those clinical data of 22 elder patients with acute cholecystitis in our hospital during the same period who were treated with traditional gallbladder removal operation, and these data were evaluated with statistical analysis. Results: The incidence rate of postoperative complications and death rate in the group of elder patients with acute cholecystitis who were treated with PGCD were 0, the average stay was 18.2 days. The incidence rate of postoperative complications in the group of elder patients with acute cholecystitis who were treated with traditional gallbladder removal operation was 40.9% , the death rate of this group was 13.6% , and the average stay was 23.7 days. Statistical analysis confirmed that the difference of incidence rate of postoperative complications, death rate and average stay between these two treatments have statistical significance. Conclusion: Ultrasonography guiding PGCD is a safe, effective and easily operating method for the first treatment plan treatment of elder patients with acute cholecystitis.

Objective:To explore the correlation between lymph node metastasis rate and prognosis in non-small cell lung cancer (NSCLC) patients with stage Ⅲ A/N 2. Methods:The clinical data of 350 NSCLC patients with stage Ⅲ A/N 2 receiving surgical treatment from January 2012 to June 2015 in Shanxi Provincial Cancer Hospital were retrospectively analyzed. The patients were divided into the high lymph node metastasis rate group and the low lymph node metastasis rate group according to the optimal cutoff value of lymph node metastasis rate determined by Cox hazard regression model. The relationship between clinicopathological features and lymph node metastasis rate was analyzed. Prognostic risk factors were analyzed by using Cox regression model, and overall survival (OS) and disease-free survival (DFS) of both groups were analyzed by using Kaplan-Meier method. Results:The optimal cutoff value of lymph node metastasis rate was 0.2. There were 180 cases with high lymph node metastasis rate (>0.2) and 170 cases with low lymph node metastasis rate (≤0.2). The proportion of the patients with adenocarcinoma and the highest lymph node metastasis in the high lymph node metastasis rate group was higher than that in the low lymph node metastasis rate group [72.2% (130/180) vs. 52.9% (90/170), 52.8% (95/180) vs. 29.4% (50/170), all P<0.05]. The proportion of patients with jumping N 2 lymph node metastasis, single station N 2 lymph node metastasis and single station N 2 lymph node metastasis in the highest group was lower than that in the low lymph node metastasis rate group [51.1% (92/180) vs. 71.8% (122/170), 25.0% (45/180) vs. 44.1% (75/170), 38.9% (70/180) vs. 75.3% (128/175), all P<0.05]. Cox multi-factor regression model analysis showed that adenocarcinoma, multiple stations N 2 lymph node metastasis, lymph node metastasis rate were independent risk factors of DFS for NSCLC patients with stage Ⅲ A/N 2 ( HR = 2.201, 95% CI 1.444-3.355; HR=2.971,95% CI 1.950-4.529; HR=3.543, 95% CI 1.874-6.699; all P<0.05). Lymph node metastasis rate was an independent risk factor of OS for NSCLC patients with stage Ⅲ A/N 2 ( HR = 3.669, 95% CI 1.941-6.938, P<0.05). The 5-year OS rate of the low lymph node metastasis rate group was higher than that of the high lymph node metastasis rate group (64.00% vs. 36.58%, χ2 = 11.422, P = 0.001). The 5-year DFS rate in the low lymph node metastasis rate group was higher than that in the high lymph node metastasis rate group (45.00% vs.18.32%, χ2 = 13.624, P<0.01). Conclusion:Lymph node metastasis rate is an independent influencing factor for the prognosis of NSCLC patients with Ⅲ A/N 2 stage, and it can effectively evaluate the prognosis.

Objective To improve the diagnosis of metastatic adrenal carcinomas. Methods 123 cases of primary canc-er which had been proved by autopsy in recent 10 years were reviewed. 23 cases of metastatic adrenal carcinomas werefound in all cases,the metastatic being unilateral in 20 and bilateral in 3. Results All the 23 cases of metastatic adrenal-carcinomas had been found missed diagnosis. Conclusion 16 cases are from lung cancer,among which 8 cases are smallcell lung cancer. It is important to prevent metastatic adrenal carcinomas from small cell lung cancer.

No interstitial bleeding occurred in either type of grafts. By gross observation and under light microscopy, a smooth neointima was noticed on the surface of collagen coated grafts 30 and 90 days after implantation. In a series of 4 patients with Marfan's syndrome undergoing replacement of the aortic root, hemorrhage through the interstices of the graft was virtually eliminated. There was no complication in any patient after a follow up of 3 months. This study reveals that collagen-coated dacron grafts were totally impervious to blood during and after implantation. The collagen coating can promoting cell growth and graft healing and does not interfere with the physical properties of the dacron grafts. As a prosthesis of aorta replacement , it may be applied clinically with safety. If it is used as small diameter vascular prosthesis,the long-term patency may be improved.

One kind of glycosaminoglycans (GAG) was extracted and purified from the ink of Sepiella maindroni de Rochebrune by using the procedures of trypsin and pronase digestion, ethanol precipitation and DEAE-cellulose inon-exchange chromatography. It was proved to be homogeneous by polyacrylamide gel and cellulose acetate membrane electrophoresis. No protein absorption was shown in UV spectrum, but typical absorption of GAG was shown in the IR spectrum. It was identified by paper and thin layer chromatography of the acid hydrolyzate that the GAG was composed of galactosamine, glucuronic acid and fucose. The percentage content of galactosamine, glucuronic acid, fucose, sulfate and protein in the GAG was 25. 6%, 29.2%, 23.3%, 8.2% and 3. 5%, respectively.

Objective To develop LabVIEW-based digital PCG analyzer. Methods A kind of Digital PCG analyzer based on LabVIEW was designed. It included a heart sound sensor, an analog filtering circuit and amplifying circuit, an AD converter, a digital data transferring model and a software for PCG analyzing. Meanwhile, a kind of 3-lead ECG sampling circuit and a display model in LabVIEW were designed as a reference signal for PCG. distinction. Results This digital PCG analyzer had the functions of heart sound sampling from body surface, PCG real-time displaying on the software, spectrum analyzing for the PCG data and PCG database managing. Conclusion This kind of digital PCG analyzer based on LabVIEW can be used for assistant diagnosis of cardiac Arrhythmia and cardiac abnormal noise, and as a teaching tool for clinical auscultation.

Objective To study the drug retention of HPC MMC in bladder after intravesical instillation. Methods The pharmacokinetics of the intravesical mucous adhesive anticancer agent HPC MMC in urine and in tissue were comparatively studied with conventional mitomycin C aqueous solution(S MMC) by means of animal experiment. Results After instillation, the half life of MMC in urine in the 2 groups was 2.61 h and 0.56 h and in tissue 2.33h and 0.46 h respectively. Conclusions Suggesting that HPC MMC remained longer within the urinary bladder. It may be therapeutically more efficient.

Objective To evaluate the clinical value bronchofiberscope and perfusion in the treatment of multi- drug resistant pulmonary tuberculosis (MDR-TB).Methods Forty-two cases were treated by injecting amikacin (0.4 g), ofloxacin (0.4 g), after cleaning up exudation through bronchofiberscope and catheter intervention combined with chemotherapy therapy, while 37 controls were treated by chemotherapy only.Results By the end of 9 months, the sputum tubercle bacillus conversion rate, radiographical improvement rate were 92.9% and 90.5% respectively in the treated groups,while all these indications were higher than those of control group 62.2% and 59.5% (P

Objective To investigate the frequencies of variant of lipoprotein lipase(LPL)gene,HindIII site at intron 8 and PvuII site at intron 6,and its relation with type 2 diabetes mellitus and insulin resistance(IR).Methods PCR-RFLP method was used to determine the DNA polymorphism of 8 th intron and 6 th intron of LPL gene in 78 T2DM patients and 49 controls.Results(1)Compared with control group,the frequencies of H+H+ genotypes and alleles ″H+″ was significantly higher in type 2 diabetes patients(P

To probe course and mechanism of cellular immune respone in early syphilitic lesion, the expression levels of CD3, CD20, CD68 were determined by immunohistochemistry and a computer image analysis system. The results showed that the expressions of CD3, CD20, and CD 68 were positive in 43 cases of primary and secondary syphilis. The expression level of CD68 was higher thanthat of CD3, CD20, especially around blood vessels. The expression level of CD 20 was very low. This study indicates that T cells, B cells, and in particular macrophages play an important cellular immunologic regulating role in clearance of Treponema pallidum from the infected tissue, but the efficacy is not high enough.