Selenium, iodine and zinc are the essential trace elements for human body and the important components of many kinds of hormones, proteins and enzymes. The three elements can play important biological roles by affecting the hormones, proteins and enzymes in human body, one of the three elements can influence the biological effects of the other two. For preventing and curing some deficiency diseases and supernutrition, to study the correlated biological effect of the three trace elements will demonstrate the important theory significance and practical value.

Objective To investigate the clinical application value of fluorescence PCR in vitro diagnosis(IVD)reagent kits in the HLA-B27 detection by comparing 2 kinds of HLA-B27 IVD reagent kit approved by CFDA.Methods A total of 573 clinical blood samples were collected and detected for HLA-B27 by the approved reagent kits based on the fluorescence PCR technique and the flow cytometry.The samples with inconsistent testing results by the two kits were further confirmed by the PCR sequencing.At the same time,about 5% samples of the positive results detected by the fluorescence PCR method were extracted for conducting the re-testing.Results Among 573 samples,191 samples were HLA-B27 positive and 382 cases were HLA-B27 negative by flow cytome-try;the same samples had 194 cases of HLA-B27 positive and 379 cases of HLA-B27 negative by real-time PCR.With flow cytome-try as reference of the final results,the positive coincidence rate of the two kinds of kit was 96.33%(184/191),the negative coinci-dence rate was 94.76%(362/382),27 samples had inconsistent results from the two kinds of assay(accounting for 4.71% of the to-tal number of samples),the total coincidence rate was 95.29% [(184+362)/573],the Kappa value was 0.896(P =0.02);the chi-square test P =0.021,the two kinds of testing method had the high consistency,but the differences existed in the testing results. The re-testing results by PCR sequencing(including 27 samples with inconsistent results by two kinds of kit)were entirely consist-ent with the fluorescence PCR testing results.Conclusion Compared with the authority method flow cytometry for HLA-B27 tes-ting in clinic,the fluorescence PCR kit may present more accurate judging ability for the HLA-B27 testing on the basis of ensuring the higher consistency of the testing results,is easier compared with the sample preparation and operating procedures,and has the stronger clinical application value and prospects s.

BACKGROUND:Interleukin-1βand matrix metal oproteinase-13 can promote the metabolism of chondrocytes, inhibit the capacity of synthesizing and repairing, induce the degradation of extracellular matrix, and play a crucial role in the occurrence of osteoarthritis.
OBJECTIVE:To observe the effects of extracorporeal shock wave therapy on the interleukin-1βand matrix metal oproteinase-13 expression in rabbits with experimental knee osteoarthritis.
METHODS:Thirty New Zealand rabbits were randomly and equal y divided into treatment group, model group and control group, with 10 rabbits in each group. Model of knee osteoarthritis was established in both the treatment group and model group, using modified plaster cast in extension position for 6 weeks. Then the rabbits of treatment group were treated with extracorporeal shock wave therapy, each 1 000 impulse, at the energy flux density of 0.1 mJ/mm2. There were no treatments in the control group. The rabbits in each group were sacrificed at 4 weeks after treatment, the knee synovial fluid and articular cartilage were col ected from the rabbits. The pathological changes of knee joint were detected using hematoxylin-eosin staining and toluidine blue staining. The interleukin-1βand matrix metal oproteinase-13 expression in the synovia was detected using ELISA and immunohistochemical staining respectively.
RESULTS AND CONCLUSION:The interleukin-1βconcentration in the synovial fluid was significantly higher in the treatment group and model group than the control group (P<0.01), and the treatment group after treatment showed a lower concentration than the model group (P<0.05). Mankin scores in treatment group and model group were significantly increased compared with the control group (P<0.01), and the treatment group after treatment showed a lower score than the model group (P<0.05). The interleukin-1βand matrix metal oproteinase-13 positive expression rates in the treatment group and model group were significantly increased compared with the control group (P<0.01), and the treatment group after treatment showed a lower rate than the model group (P<0.05). The extracorporeal shock wave therapy can downregulate the expression of interleukin-1βand matrix metal oproteinase-13, promote the synthesis of new col agen.

Objective To research the growth of human umbilical vein endothelial cell(HUECV) cultured with different dose of selenium. Methods HUVEC were cultured with selenium in various concentration (50、100、500、1000、2000 nmol/L) for 48 h in vitro. The morphological changes of cells were observed, activity of cell was measured by MTT method, and at the same time the levels of SOD, GSH-Px, MDA, NO, iNOS, eNOS in the culture were determined. Results At the doses of 1 000-2 000 nmol/L, the cells were damaged and revealed related morphological change. Cell activity was significantly higher at the dose of 100 nmol/L compared with that of control (P

Objective:To investigate affection of ureaplasma urealyticum (UU) infection on the production of calculus of the urinary and reproductive system in male rat. Methods:Sprogue-dawley(SD) rats were infected with UU4 (serotype 4) through repeated natural sexual intercourses for 8 weeks. The urinary and reproductive system were detected . Results:Twelve point five percents of rate infected with UU had soft calculus in urinary tracts, while 27.5 percents of rate infected with UU had soft or hard calculus. Conclusion: Infection with UU may lead to the production of calculus in urinary and reproductive system in male rats

Objective To explore the effect of extracorporeal shock wave (ESW) on expression of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) in cartilage in rabbits with knee osteoarthritis. Methods 30 rabbits were randomly divided into control group, model group and treatment group, with 10 rabbits in each group. Model of knee osteoarthritis was established with modified plaster cast in extension position for 6 weeks except the control group, and the treatment group was treated with ESW (each 1000 impulse, the energy flux density of 0.1 mJ/mm2). The rabbits were sacrificed respectively 4 weeks after ESW treatment, the general and histological changes of articular cartilage were examined and immunohistochemical staining was used to observe the expression of IL-1β and TNF-α in cartilage. Results The expression of IL-1β and TNF-α were significantly lower in the treatment group than in the model group (P<0.01). Conclusion ESW can down-regulate the expression of IL-1β and TNF-α in chondrocytes with osteoarthritis.

Objective To evaluate the different effeotions among laparoseopic choleeysteotomy (LC)combined with bile duct exploration (LCBDE)and stone removal,LC with endoacopic sphincterotomy (EST),and open-choleeysteetomy (OC)with exploration of common bile duct (ECBD)for treating choleeys-tolithiasis with choledocholithiasis.Methods Among 289 cases of choleeystolithiasis and choledocholithla-sis,132 patients were treated by OC with ECBD,36 cases by LC with LCBDE,121 cases by EST combined with LC.The stone residual rate and leakage of bile rate,operation time,the loss of blood in operation,re-covery time of gastrointestinal function,length of hospital stay of the three groups were compared.Results There was no statistical difference in the stone residual rate and leakage of bile rate among the three groups,but OC with ECBD group had significantly longer operation time,more loss of blood in operation,later re-covery time of gastrointestinal function and longer hospital stay than the other groups.Conclusions There are respective indications,advantages and disadvantages in the three groups.We found that the operation of LC with LCBDE is the better choice for the patients of cholecystolithiasis with choledocholithiasis,single choledocholithiasis;However,the patients with obviously infection of biliary tract or acute pancreatitis is not suitable for this method.

Objective: To investigate the antitumor actions of polysaccharide extracts from the fruiting body of coriolus versicolor (CVE). Methods:Hepatoma HepA cells were injected into mice subcutaneously. Different doses of CVE were given by gavage. On the 7 th and 14 th day, tumor inhibitive rates were calculated. ELISA was performed to measure the serum IgG level; MTT was used to examine CVE′s effects on the proliferation of T lymphocytes of thymus. Immunohistochemistry was used to determine CVE′s influence on the expression of tumor related genes P53 and VEGF in liver. Results: CVE may evidently inhibit the growth of the transplanted HepA tumors. Its effects on the serum IgG level and on the proliferation of T lymphocytes of thymus were also significantly. Also, CVE markedly decreased the expression of P53, VEGF genes in liver. Conclustion: CVE had significant antitumor effects in vivo . The mechanisms may involve immune modulation effects and antimetastasis actions.

ObjectiveTo evaluate the role of transurethral resection of the prostate (TURP) in the management of prostate carcinoma.MethodsTURP were randomly performed on 19 suspected patients with prostate carcinoma, based on their mean serum PSA level (14.5 ng/ml) and negative result of anal touch,so as to take more biopsy samples and to make histopathologic diagnosis.Meanwhile,another 8 patients with prostate carcinoma underwent palliative TURP so as to resolve bladder outlet obstruction.ResultsOf the 19 suspected patients, 6 (33%) were pathologically diagnosed to have prostate carcinoma.These 6 patients were all alive during a follow-up for 3 months to 6 years.Obstructive symptom was relieved in 4 patients who underwent palliative TURP,but repeat palliative TURP was needed in 2 patients 3 months after initial operations.ConclusionsIn certain circumstances,TURP may play a role in the diagnosis of prostate carcinoma. In addition, it relieves obstructive symptom caused by enlarged prostate. However, the long-term effects are unpredictable.

Objective To detect the changes of gastric emptying function by gastroenteric contrastenhanced ultrasonography and to offer the evidence for the diagnosis and treatment of diabetic gastroparesis.Methods The gastric emptying function was measured by gastroenteric contrast-enhanced ultrasonography in 35 cases of diabetic gastroparesis (DGP,DGP group) and 35 volunteers (control group).The gastric emptying rate(GER) and emptying time(ET) after taking orally gastroenteric ultrasound developer in DGP group were observed and compared with control group.Results The GER in DGP group was lower than that in control group [(27.05 ± 11.32)％ vs.(45.38 ± 15.08) ％] (P ＜ 0.05).The ET in DGP group was longer than that in control group[(102.43 ± 15.76) min vs.(75.31 ± 11.52) min] (P ＜ 0.05).Conclusions The method of gastroenteric contrast-enhanced ultrasonography is a simple,and repeatable imaging method,which can be used to make a real-time assessment on the gastric dynamic changes,but don't influence gastroenteric function and can provide a dependent and noninvasive examine means for clinical physician to test the gastric emptying function in DGP.