Objective To study the epidemical status of notifiable infectious diseases in navy from 1992 to 2006,in order to set a scientific basis for working out the measures of preventing from infectious diseases.Methods The reported data were classified by the current national approach and descriptively analyzed concerning infectious diseases during a period of 1992 to 2006 based on the Infectious Disease Surveillance and Reporting System of Chinese PLA.Results 6626 cases of 34 different notifiable infectious diseases were reported in PLA Navy during 1992-2006.Class A,B and C notifiable infectious disease were all reported.There was one death case.Viral hepatitis,dysentery and pulmonary tuberculosis ranked the top situation composing 78.84% of the total cases.The types of infectious diseases during 1992-1999 were less than that during 2000-2006,while the incidence was opposite.Gastrointestinal diseases were the major infectious diseases,accounting for 50.89%.The reported incidence of gastrointestinal diseases declined,and that of respiratory diseases ascended.Blood-borne and sexually transmitted infectious diseases accounted for 18.04%.Natural focal and arthropod-borne disease accounted for 4.95%.Conclusion Certain achievements in prevention and control of infectious diseases have been made since the present century.However,gastrointestinal and respiratory diseases should remain to be regarded,especially for viral hepatitis,pulmonary tuberculosis,dysentery,chicken pox and mumps.In the meantime the propaganda and education on the knowledge of disease prevention should be strengthened among the focus groups.

Recent studies suggest that astrocyte elevated gene 1 ( AEG-1 ) is almost highly expressed in all types of malignant solid tumors and correlates with poor prognosis,which becomes a prognostic marker for many kinds of tumors.As a strongly basic protein,AEG-1 possesses a transmembrane domain and multiplenuclear localization signals.It is present in the cell membrane,cytoplasm and nucleus.As an oncogene,AEG-1 palys an important role in a virety of malignant biological behaviors of cancer,which range from cell proliferation,apoptosis,migration,adhesion,invasion to tumor angiogenesis and chemotherapy resistance.Its critical role in tumor genesis and progression has made it a potential therapeutic target.

目的探讨应用非手术方法治疗老年患者腰椎管狭窄症的疗效。方法对74例老年(>60岁)腰椎管狭窄的患者采用手法矫正椎体的假性滑移、药物治疗、适当的腰背肌功能锻炼综合治疗。随访至少2年。采用自定标准评定疗效。结果优(无疼痛及间歇性跛行,无功能限制,不用止痛剂)34例,良(偶然疼痛及间歇性跛行,轻度功能受限,偶尔使用止痛剂)27例,一般(中度的疼痛及间歇性跛行,中度功能限制,无运动,经常使用止痛剂)11例,差(中度每天疼痛及间歇性跛行,或偶尔严重疼痛,每天日常生活活动受限,应用强止痛剂)2例。结论对老年腰椎管狭窄症采取综合的非手术方法能取得一定疗效。

Objective To explore the clinical efficacy of biapenem treatment of in hepatic failure complicated with bacterial infection.Methods 30 patients with hepatic failure complicated with bacterial infection were randomly divided into two groups.Treatment group were administered biapenem(Zhengda Tianqing Pharmaceuticals)0.6g/d,while the control group were administered cefopergone sodium tazobactam sodium for Injection(Qili Haikou Pharmaceuticals)4.0g/d for one week.Results There were significan differences in the curative rate and effective rate of biapenem and cefopergone sodium tazobactam sodium,which were 86.67％ 73.33％ and 53.33％ 、33.33％respeitively(P＜0.05).Conclusion Biapenem in treatment of hepatic failure complicated with bacterial infections was effective against infection with mild adverse reactions.

Objective To investigate the effect of endoplasmic reticulum stress in renal damage caused by hyperlipidemia. Methods Forty male Wistar rats were randomly divided into two groups: normal control group (NC, n=20) and high-fat group (HF, n=20). Rats in NC group were fed with normal diet while those in HF group were fed with high-fat diet. Five rats in each group were randomly chosen in week 4, 8, 12 and 18. Serum lipid, urine protein in 24 hours and the pathological changes of renal tissues were observed; the apoptosis of renal cells was detected by TUNEL staining; the expression of GRP78 protein in the kidney was examined by immunohistochemistry. The expression of GRP78 mRNA and CHOP mRNA was examined by RT-PCR. Results Compared with those in NC group, serum lipid as well as the expression of GRP78 mRNA and protein in the kidney were increased in week 4, 8, 12 and 18 in HF group(P<0.05). In contrast, urine protein in 24 hours, the apoptosis index of renal cells and the expression of CHOP mRNA were increased in week 8, 12 and 18 (P<0.05). Conclusion CHOP pathway of endoplasmic reticulum stress is involved in renal damage caused by hyperlipidemia.

AIM: To investigate the role of endoplasmic reticulum stress in renal injury caused by hyperlipidemia and the influence effect of simvastatin. METHODS: Thirty male Wistar rats were randomly divided into three groups: rats in control group (n=10) were fed with normal diet; rats in high fat group (n=10) were fed with high fat diet; animals in simvastatin+high fat group (n=10) were fed with high fat diet and were received simvastatin 10 mg·kg~(-1)·d~(-1) by gastric irrigation. After 18 weeks, the quantitative urine protein in 24 h, the serum cholesterol and triglycerides levels were tested. The pathological changes of renal tissue were observed under optic microscope. The expressions of GRP78 and p-JNK in renal tissues were examined by immunohistochemistry. The apoptotic cells in the kidney were detected by TUNEL staining. The mRNA expressions of GRP78 and CHOP were examined by RT-PCR. RESULTS: The quantitative urine protein in 24 h, the serum lipid, the expressions of GRP78 and p-JNK proteins, the mRNA expressions of GRP78 and CHOP as well as the apoptotic cells in renal tissues were increased in high fat group (P<0.01).The quantitative urine protein in 24 h, the serum lipid, the expression of GRP78 and p-JNK proteins, the mRNA expressions of GRP78 and CHOP as well as the apoptotic cells in renal tissues were remarkably reduced in simvastatin+high fat group than those in high fat group (P<0.05). CONCLUSION: The endoplasmic reticulum stress is engaged in the renal injury caused by hyperlipidemia. The simvastatin play a role in renal protection by inhibiting the endoplasmic reticulum stress in the kidney.

[Objective] To investigate the effects of the bioactive constituents of green tea polyphenols epigallocatechin gallate (EGCG) on low density lipoprotein receptor (LDLR) function of HepG2 cells.[Methods] The optimal concentration and cell proliferation of HepG2 cells were determined by CCK8 assay,and Western blotting was used to determine LDLR and PCSK9 protein levels,respectively,and LDL uptake in HepG2 cells was detected by fluorescence microscope.[Results] EGCG elevated LDLR protein expression,reduced PCSK9 protein expression and promoted LDL uptake in HepG2 cells.[Conclusion] EGCG may increase LDLR abundance by down-regulating PCSK9 protein and attenuating LDLR protein degradation,which providing a new approach for lipid lowering therapy.

OBJECTIVE:To ensure a long-term medication in children with asthma,prevent the attack of asthma and ensure safe,effective and correct drug use in these patients.METHODS:The pharmaceutical care for the asthma children was carried out through pharmacists’ involvement in assisting doctors to establish the treatment plan,carrying out medicine-use education and drug use consultation for patients,setting up the medicine-use record and so on.RESULTS & CONCLUSION:The practice of pharmaceutical care can markedly improve the compliance,safety and efficacy of drug use in asthma children as well as improving their quality of life.

OBJECTIVE:To discuss the way for clinical pharmacists to carry out pharmaceutical care.METHODS:We summarized the content,method and experiences of carrying out pharmaceutical care based on our clinical practice in Cardiology department.RESULTS & CONCLUSION:To provide all-round high quality pharmaceutical care for patients,clinical pharmacists should improve their own qualities by continuous learning knowledge and theories related to their own profession as well as improving their practical skills.

Objective To explore the effect of uremic serum on apoptosis in human umbilical vein endothelial cells(HUVECs) and the intervention by recombinant human erythropoietin(rhEPO) in this process.Methods From Dec.2008 to Apr.2009,10 uremic patients and 10 healthy volunteers were enrolled in this study.HUVECs were divided into 3 groups:control group(including 10% healthy serum medium),uremic group(including 10% uremic serum medium) and rhEPO treatment group(rhEPO at 5,10 or 15 U/ml was added to 10% uremic serum medium).After 24 hous's intervention,cell apoptosis was evaluated by TUNEL assay,and reactive oxygen species were detected by colorimetry.Results HUVECs apoptosis index and ROS level were higher in the presence of uremic serum than those of healthy serum(P