The levels of salivary testosterone (ST), serum total testosterone (TT), LH, FSH and estradiol (E,) were measured with RIA in 20 men aged 21 to 47 years, with Graves' disease (GD) before and during treatment with thioureas. The results were compared with those of 20 sex-and age-matched healthy subjects. Prior to treatment, the level of ST in 20 patients (156.7?10.7pmol/L, Xr?SE) was significantly lower than that in normal males (245.8?23.8), but the levels of serum TT (49.5?4.1nmol/L), LH (29.6?3.4IU / L) and FSH (16.3?1.8IU / L) were above normal (TT 20.8?1.4, LH 15.6?1.4, FSH 9.0?1.3). With decrease of serum T, and T4 during treatment, ST gradually increased while TT, LH and FSH declined. When the patients were treated for 2 months, ST (203.5?15.6), TT (23.7?2.1) and LH (18.3 ?1.9) became comparable to controls. The levels of E2, while not significantly changed before (201.6?27.2pmol / L) and during treatment (206.8?30.8), all were higher than those in controls (119.5?12.2). There was a significant correlation between the levels of LH and those of T3 and T, (r= -0.270 and -0.267 respectively) as well, but no significant correlation between LH and ST levels(r= -0.106). The results suggested that free testosterone reflected by ST was reduced in men with GD and the concentration of ST was not influenced by SHBG.

OBJECTIVE: To determine whether serum adipocytokines and leptin level are associated with bone mineral density (BMD) in postmenopausal women. METHODS: Serum adiponectin and leptin level were measured by ELISA in 336 postmenopausal women. BMD was measured by dual energy X-ray absorptiometry (DXA) at the whole body, lumbar spine, hip, and forearm. Body compositions including lean tissue mass and body fat mass were measured by DXA, and their relationship was analyzed. RESULTS: Serum adiponectin levels were negatively related to BMD at the whole body, the lumbar spine, the hip, and the forearm (r = -0.181, r = -0.208, r = -0.228, r = -0.203, and P < 0.05, respectively). After adjustment for age and fat mass, the correlation with BMD still remained (r = -0.131, r = -0.140, r = -0.159, r = -0.172, and P<0.05, respectively). Serum leptin levels were positively related to BMD was at the hip and the forearm (r = 0.162, r = 0.210, and P < 0.05, respectively). After adjustment for age and fat mass, the correlation with BMD at the forearm remained (r = 0.157, P<0.05), but the correlation with BMD at the hip disappeared. In the multiple linear stepwise regression analysis, year since menopause, lean mass, adiponectin, and estradiol were independent predictors of BMD at the body, the hip and the forearm; year since menopause, lean mass, body mass index, adiponectin, and extradiol were independent predictors of BMD at the lumbar spine. CONCLUSION Adiponectin is the independent predictor of BMD.
Absorptiometry, Photon ; Adiponectin ; blood ; Aged ; Aged, 80 and over ; Bone Density ; physiology ; Enzyme-Linked Immunosorbent Assay ; Female ; Humans ; Leptin ; blood ; Middle Aged ; Postmenopause ; blood ; metabolism