1.Clinical application and effects of two different liposuction methods in breast augmentation by autologous fat grafting
Qian ZHANG ; Jie SHI ; Xiaodian LI ; Yaguang TIAN ; Kai TAO
Chinese Journal of Medical Aesthetics and Cosmetology 2019;25(1):25-28
Objective To explore the effect of different harvest methods of liposuction on the autologous fat grafting.Methods The clinical data of 60 patients with fat grafting for breast augmentation from January 2014 to January 2017 were analyzed retrospectively.According to the method of liposuction,60 patients were divided into the water-jet assisted liposuction group (30 cases) and negative-pressure machine liposuction group (30 cases).The surgical time of different liposuctions and the fat survival were compared after breast augmentation.The clinical effect of different liposuction methods was analyzed by follow-up one year after operation.Results Sixty patients completed the surgery.The fat survival rate of water-jet assisted liposuction was (66.71±2.68) %,and the fat survival rate of the negative-pressure machine liposuction was (51.44 ± 1.16) %.There were statistically significant difference between the two groups (P<0.05).As for the operation time of liposuction,the water-jet assisted liposuction group was (33.28 ± 2.96) min,the negative-pressure machine liposuction group was (52.91± 5.03) min;there were statistically significant difference between the two groups (P < 0.05).There was no statistically significant difference in satisfactory rate (P>0.05).Conclusions Compared with negative pressure liposuction,water-jet assisted liposuction using autologous fat grafting in breast augmentation can improve the survival rate of fat transplantation.This technique has good clinical application value.
2. Si-PTN inhibits the growth of hypertrophic scar fibroblasts
Qian ZHANG ; Jie SHI ; Yaguang TIAN ; Xiaodian LI ; Kai TAO
Chinese Journal of Plastic Surgery 2019;35(6):601-606
Objective:
To analyze the effects of PTN in the growth of hypertrophic scar fibroblasts.
Methods:
The primary scar fibroblasts were extracted from fresh hypertrophic scar tissues of 18 patients, to restrict the si-PTN cell line. After successful construction, the scar fibroblasts in logarithmic growth were divided into si-PTN group and control group, transfected with 2 μg si-PTN and control nonsense chains, respectively. MTT, PI staining and Annexin V-FITC/PI assay were used to detect the proliferation and apoptosis of si-PTN and control groups. Western blot and RT-PCR were used to detect the expression level of Cyclin D1, Bcl-2 and Bax in si-PTN and control groups.
Results:
The inhibition rates of si-PTN on the proliferation of scar fibroblasts were 14.49%, 13.24%, 20.78% and 23.12% at 1, 2, 3 and 4 days, respectively. The difference was statistically significant compared with the control group (
3.Amifostin in protection of kidney from cisplatinum injury.
Huijuan CUI ; Shujun ZHANG ; Peiwen LI ; Zhongzhen GUAN ; Xiaofei SUN ; Keng SHEN ; Ming WU ; Xiaodian HU ; Shujun LIU ; Lijun DI ; Shucai ZHANG
Chinese Journal of Oncology 2002;24(1):48-50
OBJECTIVETo evaluate Amifostin's effect on protecting kidney from cisplatinum (DDP) injury and its adverse reactions and safety.
METHODS193 Patients were divided into two groups randomly: 102 in group A (treatment group) and 91 in group B (control group). Indexes such as blood routine, blood calcium, liver function, blood urea nitrogen (BUN), cretinine (C), and urinary N-acetyl-beta-D-glucosaminidase (NAG)/C and micro-albumin (MAB/C) were monitored at different intervals before or after treatment.
RESULTSIn the two courses of treatment in both groups, the deviation (D) values of MAB/C before treatment and on D2 in group A were lower than those in grop B (P < 0.05), so were those before treatment and on D4, D6, D10 and D14 (P < 0.01). The D-values of NAG/C before treatment and on D4, D6, D10 and D14 in the first course of group A were obviously lower than those on the corresponding days in group B (P < 0.01), so were those before treatment and on D2, D4, D6, D10 and D14 in the second course (P < 0.01).
CONCLUSIONThe reduction of MAB/C and NAG/C by Amifostin in group A demonstrates that: Amifostin is able to effectively protect the renal function, regardless of the type of tumor. In contrast with group B, Amifostin in group A shows no protection for tumor in lung cancer and ovarian cancer. The main side effects of Amifostin are mild hypotension, nausea, vomiting and hypocalcemia in some patients.
Adult ; Aged ; Amifostine ; adverse effects ; therapeutic use ; Antineoplastic Agents ; adverse effects ; Cisplatin ; adverse effects ; Humans ; Kidney Diseases ; chemically induced ; prevention & control ; Middle Aged ; Protective Agents ; adverse effects ; therapeutic use
4.Antitumor efficacy of bispecific antibody mAb04-MICA to human leukemia cell K562 in vitro and in vivo
Xiaodian DU ; Fumou SUN ; Minne YUAN ; Fei WANG ; Yali LIU ; Pengzhao SHANG ; Min WANG ; Juan ZHANG
Journal of China Pharmaceutical University 2018;49(1):117-124
This study aimed to investigate the efficacy of a bispecific antibody mAb04-MICA on human leukemia cell K562 both in vitro and vivo. mAb04-MICA was previously found to posses excellent anti-angiogenic activity, and have the ability to recruit immune surveillance in tumor microenvironment. In this study, the affinity of mAb04-MICA to VEGFR2 and NKG2D was identified by ELISA. CCK8 was used to detect the effect of mAb04-MICA on K562 proliferation. The cross reactivity of mAb04-MICA to murine VEGFR2 was determined by flow cytometry assay. To evaluate the antitumor activity of mAb04-MICA,tumor volume,tumor weight and the survival of K562 tumor-bearing nude mice were analyzed. The anti-angiogenic activity was determined by immunohisto-chemistry. The results indicated that mAb04-MICA could target to VEGFR2 and NKG2D,and inhibit K562 pro-liferation specifically. Besides,mAb04-MICA showed high binding capacity to murine VEGFR2. The bispecific antibody exhibited superior antitumor efficacy to the maternal monoclonal antibody and prolonged the survival of tumor-bearing mice. The expression of Ki-67,p-VEGFR2,VEGF and CD34 in mAb04-MICA treated group was significantly reduced. The results indicated that mAb04-MICA could attenuate the phosphorylation of VEGFR2 and impair angiogenesis of the tumor microenviroment. Therefore,mAb04-MICA could be further developed as a potential tumor targeted immunotherapeutic agent for leukemia.
5. Effect of integrated nursing care mode on lower extremity deep venous thrombosis in bedridden patients
Qin HUANG ; Huimin LI ; Xiaodian ZHANG
Chinese Journal of Practical Nursing 2019;35(12):920-923
Objective:
To explore the influence of integrated nursing care mode on the incidence of deep vein thrombosis in lower extremities in bedridden patients.
Methods:
82 cases of chronic bed rest patients in our hospital were selected as clinical subjects. The time of admission was from December 2016 to December 2017. The patients were divided into control group (routine nursing, 41 cases) and observation group (medical nursing integrated nursing, 41 cases). To compare the influence of nursing intervention on the incidence of deep venous thrombosis of the lower extremities.
Results:
After treatment, the incidence of deep vein thrombosis in the control group was 24.39% (10/41), and that in the observation group was 4.88% (2/41). The difference was statistically significant (
6.Establishment of Mathematical Models for Skeletal Age Determination of Extremitas Sternalis of Clavicle in Shanxi Adolescents.
Hua Hua ZHANG ; Chen ZHAO ; Hu Yue LIU ; Na LI ; Ya Hui WANG ; Jun Hong SUN ; Li LU
Journal of Forensic Medicine 2020;36(5):631-635
Objective To develop mathematical models for skeletal age determination with multiple statistic method based on the correlation between age and the growth of the epiphysis of extremitas sternalis of clavicle in Shanxi adolescents. Methods The 562 Shanxi sternoclavicular joint samples (454 cases of modelling, 108 cases of external verification) were scanned by the thin-section computed tomography. After volume rendering was obtained, indicators such as area of epiphysis, area of metaphysis, longest diameter of epiphysis and longest diameter of metaphysis of both extremitas sternalis of clavicle were collected. Indicators such as the ratio of area of epiphysis to area of metaphysis, and the ratio of longest diameter of epiphysis to longest diameter of metaphysis of both sides were calculated. Then multiple linear regression and random forest discriminant models were used to build mathematical models for age determination of adolescents. Results The obtained indicators exhibited a strong correlation with age (r>0.85). The multiple linear regression model for males and females (all 4 indicators entering the model) based on the ratio of longest diameter of epiphysis to longest diameter of metaphysis and the ratio of area of epiphysis to area of metaphysis had an internal validation accuracy rate (±1.0 year) of over 92% and 108 cases had an external validation accuracy rate of over 70% (±1.0 year). The out of bag error rates of random forest discriminant models were less than 2% for people over 18.0 years old (≥18.0 years old) and under 18.0 years old. The external validation accuracy rates of the 108 cases were over 80%. Conclusion The regression and discriminant models established in this study have certain reliability and accuracy and can be used in age determination of Shanxi adolescents.
Adolescent
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Age Determination by Skeleton
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Clavicle/diagnostic imaging*
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Epiphyses/diagnostic imaging*
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Female
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Forensic Anthropology
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Humans
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Male
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Models, Theoretical
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Osteogenesis
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Reproducibility of Results