1. Evaluation of 5′-untranslated region amplification and sequencing for enterovirus serotypes identification diagnosis
Shihuan TANG ; Zhenghua XIE ; Duoduo LIU ; Ying YUAN ; Manjun CHEN ; Xiaodi FAN ; Xixia DING ; Nan YU
Chinese Journal of Experimental and Clinical Virology 2018;32(5):488-491
Objective:
To evaluate an assay permitting amplification of target 5′-untranslated region (5′-UTR) sequences directly from clinical specimens and distinction among serotypes of enterovirus (EV).
Methods:
A total of 518 rectal swabs and 148 nasal swabs tested positive by pan-enterovirus real-time PCR were collected. 5′-UTR and the viral protein 1 (VP1) gene fragments were amplified and sequenced separately for serotyping. The inconsistent samples by 5′-UTR and VP1 serotyping were further determined by using the serotype-specific RT-PCR.
Results:
A total of 553 (83.0%) samples were detected by 5′-UTR serotyping and 318 (47.7%) were detected by VP1 serotyping in all 666 positive specimens, and there was significant difference in the detection rates between two methods in rectal and nasal swabs (
2.Effectiveness and safety of holmium laser therapy via flexible bronchoscopy in 40 children with tracheobronchial tuberculosis
Chao WANG ; Xia LIU ; Xiaodi TANG ; Xuelian CHANG ; Xinfeng WANG ; Chen MENG
Chinese Journal of Pediatrics 2020;58(10):824-827
Objective:To explore the effectiveness and safety of holmium laser treatment via flexible bronchoscopy in children with tracheobronchial tuberculosis (TBTB).Methods:The clinical data of 40 children with TBTB admitted in Qilu Children′s Hospital of Shandong University from February 2016 to June 2019 were retrospectively analyzed. According to the treatment approach, they were divided into conventional treatment group (20 cases) and holmium laser treatment group (20 cases). The sex, age, course of disease and lesion location before treatment, and the time till relief of atelectasis and airway obstruction after treatment were compared between the two groups with t test and χ 2 test. Results:Among the 40 cases, 24 were males and 16 females, and 35 had lymph node fistula (87.5%) and 5 had granulation proliferation (12.5%). There was no statistically significamt difference between the conventional group and laser group in sex (male ratio: 50%(10/20) vs. 75%(15/20), χ 2=2.66, P=0.10), age ((3.2±2.2) years vs. (2.2±1.8) years, t=1.41, P=0.16), course of disease (<1 month ratio: 30%(6/20) vs. 35%(7/20), χ 2=0.11, P=0.73), lesion location (single lesion ratio: 45%(9/20) vs. 60%(12/20), χ 2=0.90, P=0.34). The effectiveness was evaluated after 3 months, and the total effective rate were 100% in laser group and 40% in conventional group, the difference was significant (χ2=25.34, P<0.01). No serious adverse events occurred in the two groups during the observational period. Conclusions:Holmium laser treatment via bronchoscopy is an effective way to remove the tuberculosis foci in the trachea, which can reduce the incidence of further related complications, and could be applied in clinical approach.