To establish a spectrophotometric method for detecting selenium in drinking water,some subjects,such as the features of absorption spectrum of the reacting product of selenium and diaminonaphthalene,disturbance of other coexisting ions,detection range,detection limit,precision and accuracy were studied.The results showed that:the maximum absorption wavelength of the reacting product of Se4+and 2,3-diaminonaphthalene was 377 nm,the detection limit,molar absorptivity (ε377) and linear range of this method was 0.018 μg/50 ml,2.7×104 L/(mol·cm)and 0.2～10 μg/50ml respectively.The regressive equation was A＝0.077C＋0.014 with a correlation coefficient r value of 0.9999.The relative standard deviation (RSD) and recovery rate were ＜54.7% and 96.5%～106% respectively.This method was simple,sensitive and accurate,could meet the requirement of the routine monitoring of selenium in drinking water.

Objective To study the relationship between anti-? 2-glycoprotein I(a? 2GPⅠ) and extracellular matrix (ECM) in patients with post-hepatitis B cirrhosis (PHBC).Method The of patients with chronic hepatitis B (CHB) and PHBC were studied for the serum a? 2GPⅠ levels by ELISA with purified ? 2GPⅠ as antigen, and we investigated serum level of collagen IV (IV-C)?hyaluronic acid (HA) and laminin (LN) in these patients.Result High positive rate was observed compared with control group (P

Objective To explore the method for simultaneous determination of arsenic and mercury in drinking water. Methods Atomic fluorescence spectrometry was applied for simultaneous determination of arsenic and mercury in drinking water. The best working conditions of the determination, such as the height of flame of atomizer, current of hollow cathode lamp, reducer, acidity and required amount of NaBH 4 were defined in this assay. Results The determination range of arsenic, the correlation coefficient and detection limit for arsenic were 0～40 ng/ml,0 999 5 and 0 10 ng/ml respectively, and were 0～80 ng/ml, 0 999 3 and 0 02 ng/ml for mercury respectively. The precisions and recovery rates of the simultaneous determination of arsenic and mercury in drinking water were 0 94%～5 61% and 94%～110%. Conclusion This method with advantages such as simple, rapid, sensitive, high accuracy and less interference, was suitable for simultaneous determination of arsenic and mercury in drinking water.

Objective To explore the clinical values of strain rate imaging(SRI) in detection of regional function of left ventricular in patients with hypertension.Methods Twenty-two healthy volunteers and 30 patients with hypertension were examined with SRI.Strain rates of left ventricular segments along axis were analyzed by strain curves,and the peak SR at systole,isovolumic relaxation phase,early diastole and atrial contraction were calculated.(Results Compared) with the healthy subjects,patients with hypertension had appreciably lower SR on all walls in systole((P

Objective To investigate the efficacy and mechanism of compound glycyrrhizin on patients with serious hepatitis. Methods Thirty patients who were hospitalized from August 2005 to June 2007 with diagnosed with serious hepatitis were enrolled into treatment group and treated by compound glycyrrhizin injection ( 80 to 100 ml per day,for 3 consecutive weeks) and common supporting medicines,while the other 30 patients in control group were treated only with same supporting medicines. Mortality,biochemical parameters, plasma levels of endotoxin and inflammatory factors in patients of both groups were observed during the treatment. Results By the end of three-week of treatment,8 patients in the treatment group died with the mortality of 26. 7% ( 8 /30) . Thirteen patients died in the control group and the mortality was 43. 3% ( 13 /30) . Serum ALT and AST levels in treatment group were significantly lower than those of control group during the treatment. The average level of serum total bilirubin and plasma prothrombin time in treatment group was lower than those of control group by end of the third treatment week. The level of TNF-alpha in treatment group was lower than that of control group during treatment. The levels of plasma endotoxin and interleukin-6 in treatment group were significantly lower than those of the control group at the second and third treatment week. Conclusion Compound glycyrrhizin improves the biochemical parameters of patients with serious hepatitis,and probably,improves the survival of patients with severe hepatitis. The implying mechanism might be that compound glycyrrhizin declines plasma endotoxin levels and lessen cytokine-induced secondary hepatic injuries.

Objective: To optimize the ultrahigh pressure extraction ( UPE) process for forsythoside from Forsythia suspensa by Box-Behnken experimental design. Methods:On the basis of single factor screening, a three-factor and three-level Box-Behnken ex-perimental design was employed with liquid/material ratio( X1 ) , extraction pressure ( X2 ) and extraction time ( X3 ) as the independent variables. The response variable was the extraction yield of forsythoside. Results:The optimal UPE conditions for forsythoside were as follows:the ratio of solvent to material (ml·g-1) was 70, the extraction pressure was 151 MPa, and the extraction time was 114 s. With the optimal extraction process, the extraction yield of forsythoside was 13. 15 mg·g-1 . Conclusion:As a novel extraction tech-nology for Chinese herbal medicines, UPE procedure has higher extraction yield, lower extraction temperature, shorter extraction time and less power consumption, which provides a brand-new method for the extraction of forsythoside from Forsythia suspensa.

Objective To evaluate the values of integrated backscatterr (IBS) and dobutamine stress echo- cardiography (DSE) in the diagnosis of coronary artery disease(CAD).Methods Thirty-one suspected CAD patients received DSE and IBS analysis before selective coronary angiography(SCA).Using SCA results as the golden standard of the diagnosis,the diagnostic sensitivity(Se),specificity(Sp),and accuracy rate (Ac) of conventional eye-view and DSE-cyclic variation integrated backscatter (CVIB) were compared. Results The CVIB of myocardial segments perfused by stenotic coronary artery was smaller than that of segments perfused by patent coronary artery during high dose of DSE. For conventional DSE eye-view method, the Se,Sp,and Ac were 80%,72% and 77%, while that of DSE-CVIB were 90%,72% and 83%, respectively. Conclusion IBS is a valuable method in assessing the myocardial wall motion in DSE, and the combination of the two methods can improve the diagnostic accuracy rate of CAD.

0.05).The transfection efficiencies were improved significantly in the ultrasound plus contrast agent and VEGF plasmid group(MI 1.2,1.4,and 1.6) compared with pure plasmid group(P0.05);there were significant differences between ultrasound plus contrast agent and VEGF165 plasmid(MI 1.4,1.6) and ultrasound plus contrast agent and VEGF165 plasmid group(MI 1.2)(P

Sinacalia davidii (Franch) Koyama. grows only in China, its chemical constituents have never been studied before. The compounds were isolated by column chromatography on silica gel and Sephadex LH-20, and the structures were identified on spectroscopic data (MS, 1H-NMR, 13C-NMR). Six compounds were isolated from S. davidii, and were characterized as p-sitosterol (1), 3-oxo-2alpha, 23-dihydroxy-olean-12-en-28-oic acid (2), 28-hydroxy-olean-12-en-3, 11-dione (3), 3beta-methoxy-olean-11-oxo-18-ene (4), luteolin (5) and 2alpha-hydroxy-ursolic acid (6). All the six compounds above were isolated from S. davidii for the first time.
Asteraceae ; chemistry ; Chromatography, High Pressure Liquid ; Magnetic Resonance Spectroscopy ; Mass Spectrometry ; Organic Chemicals ; analysis ; isolation & purification

Objective To observe the mortality of fragile hip fractures and evaluate the death-associated risk factors.Methods 100 men and 186 women aged 50 to 97 (mean,77.09± 10.65) years old who had fragile hip fracture over 50 years old from 2010 to 2012 were followed up,and the clinical data were retrospectively analyzed.Three months,one year and the total mortality of following time were calculated.Mortality-related risk factors were evaluated including age,gender,and surgery,duration from injury to operation,pulmonary infection,number and kind of complications.Results The 286 patients were followed up between 6 months and 42 months,with 21.42±9.88 months in average.The three month mortality was 7.69％,the patients who were followed up over one year were 231 cases,the one year mortality was 16.02％,and the total mortality of following time was 17.48％.The mortality was associated with age,gender,surgery,duration from injury to operation,number of complications,pre-injury cardiovascular disease and respiratory system diseases,and pulmonary infection.A Binary Logistic Regression analysis revealed that the independent risk factors affecting the mortality included age (OR=5.385,P=0.003),surgery (OR=21.217,P=0.000),number of complications (OR=9.038,P=0.000),pre-injury cardiovascular disease (OR=3.201,P=0.041).Conclusion The early mortality of fragile hip fractures was high and was associated with many risk factors.Age,surgery,number of complications and pre-injury cardiovascular disease were the independent risk factors affecting the mortality of fragile hip fractures.The positive treatment with complications,early surgery in condition allowed,can lower the early mortality.