ObjectiveTo compare the results of Verme ul en calculation and the enzyme immunoassay in the determination of serum free tes tosterone (FT)concentration in aging males.Methods129 healthy males over 45 years of age who have no disease or medicine that can infl uence the secretion of hormone were enrolled in the study. They were divided int o 4 age groups. The serum specimens were prepared and kept under -40 ℃ until as saying. All the samples were divided into two parts, one measured by enzyme immu noassay and the other calculated with the Vermeulen formula after determining th e total testosterone(T)and sex-hormone-binding globulin(SHBG):FT=T- 23.43FTSHBG-(T-23.43FT)?10 -9 mol/L.The data were analyzed by S PSS.ResultsThe results of FT in the 129 males were (46 .69?21.79)pmol/L by enzyme immunoassay and (396.30?317.04)pmol/L by calculatio n.Pairing test suggested that " t " was 13.01 and the difference of FT w as remarkably significant ( P

AIM:To explore the changes of excitatory amino acids and N-methyl-D-aspartate receptor (NMDAR) in brain undergoing ischemia-reperfusion injury following hepatic portal occlusion (HPO) in vivo.METHODS: The electrolytes and pH of portal vein blood, the content of Glu and Asp in brain, and the expression of NMDAR mRNA were studied with blood-gas analysis, HPLC and semi-quantitative RT-PCR method in two groups(HPO or sham), respectively. Three time points(6 h,12 h and 24h after reperfusion) were included.RESULTS: HPLC measure showed that the content of cortex Glu and Asp in group HPO elevated significantly after reperfusion compared with sham group, the peaks were at 6 h and 24 h Glu:(349?145) ?g?g-1wt,(456?203) ?g?g-1wt vs (238?24) ?g?g-1wt,(225?59) ?g?g-1wt;Asp:(134?50) ?g?g-1wt,(166?50) ?g?g-1wt vs (99?24) ?g?g-1wt,(71?20) ?g?g-1wt.Moreover, semi-quantitative RT-PCR analysis discovered that the expression of NMDAR mRNA increased significantly , the subunit NR1 mRNA was higher in reperfusion 6 h and last to 12 h(1.63?0.06 vs 1.18?0.05; 1.73?0.06 vs 1.17?0.03), the peak of NR2B mRNA was in 12 h (1.75?0.04 vs 1.18?0.05) ,but they did not further increase after reperfusion for 24 h.CONCLUSION: HPO increases the content of Glu and Asp and the expression of NMDAR in brain cortex. The Glu-NMDAR pathway plays a role in the mechanism for the brain injury after HPO. The products of IR injury might be the key factor to cause EAAs acumulation in synapse space.

Postural tachycardia syndrome (POTS) is one type of orthostatic intolerance.The treatment for POTS including non-drug treatment and medications,such as α-receptor agonists,β-recepter blockers and oral rehydration salts.The prognostic meaning of biomarkers and hemodynamic parameters in the POTS children treated with midodrine hydrochloride are discussed in this paper.

Objective To evaluate the efficacy and safety of emergency percutaneous coronary intervention(PCI) in patients with non-ST-segment elevation acute coronary syndrome(ACS).Methods A total of 233 patients(emergency group) were treated with emergency PCI within 48 h of heart attack and another 152 patients(delayed group) were treated with PCI after 3-14 days of medical therapy.All culprit lesions were treated.Procedural success rate,the time from admission to angina relief,the length of hospital stay and cardiac events incidence in 30 days were observed.Results The procedural success rates for the emergency group and the delayed group were similar(98.1% vs 95.5%),but cardiac events incidence in 30 days was significantly lower in the emergency group than that of the delayed group(2.9% vs 14.1%,P

Dermoid cysts occur in the maxillary sinus mucosa were rare. Patient's CT showed: maxillary sinuses Sinus cavity expansion, sinus wall thinning. The inside of the sinus wall disappeared, the maxillary sinus filled with soft tissue mass shadow. Bacterial culture: Staphylococcus aureus. Pathological report: a very small a mount of scattered broken squamous epithelium and keratosis, no atypia cells. Lesions consistent with epidermoid cyst. Patients with epidermoid cysts, formated probably in the process of embryonic development, the reasons of rapid growth may be considered for inflammatory stimulation.
Epidermal Cyst ; Humans ; Male ; Maxillary Sinus ; Young Adult

[ ABSTRACT] AIM:To investigate the effects of maternal limb ischemic preconditioning ( LIP) on the mitochon-drial structures and functions of the hippocampal neurons induced by reoxygenation in the intrauterine distress fetal rats. METHODS:Pregnant rats (n=40) were randomly divided into 4 groups: sham (S) group, LIP group, fetal distress ( FD) group and LIP+FD group.Intrauterine ischemia model was established through the experimental design.The ultra-structure of the mitochondria in CA1 area of the hippocampus was observed .The mitochondrial membrane potential and re-active oxygen species ( ROS) were measured .The content of ATP and MDA in the hippocampus tissue was detected.The activity of Mn-SOD was observed.RESULTS:Compared with sham group, the ultrastructure of mitochondria in CA1 area of the hippocampus was damaged in FD group and LIP+FD group.The mitochondrial membrane potential, the content of ATP and the activity of Mn-SOD were decreased.However, the content of ROS and MDA was increased.Compared with FD group, the ultrastructure of mitochondria in CA1 area of the hippocampus was intact in LIP+FD group.Furthermore, the reduced mitochondrial membrane potential and ATP content were inhibited.The activity of Mn-SOD was increased, but the content of ROS and MDA was decreased in LIP+FD group.CONCLUSION:Limb ischemia preconditioning inhibits the damage the mitochondria of fetal hippocampal neurons induced by reoxygenation in the intrauterine distress fetal rats.

after investigating the present situations of the AIDS prevalence and the prevention and treatment of AIDS in shanxi province,to analyze the problems of AIDS' social and ethics.Based on analyzing these problems,from the point of medical ethics & health Economic Ethics,aim at putting forward some counterplans,hope the prevention and treatment work of AIDS for shanxi province to promote a further Effectiveness.

Objeetive To explore the situation of rotavirus infection and extraintestinal organe damage in children in Yueqing city.Methods Two hundred and eighty-seven cases with acute rotavirus gastroenteritis in our hospital were analyzed for prospective study from October 2011 to January 2013 by stool tests.Results Rotavirus infection was found to be more common in autumn and winter.There were 223 cases (17.7％) got extraintestinal organe damage,175 cases (60.80％) got myocardial lesion,and 78 cases (27.18％) got respiratory infection.At the same time,there were 51 cases (17.77％) and 21 cases (7.31％) got liver function lesion and convulsion respectively.Among the metabolic acidosis(48 cases),39 cases were combined with myocardial lesion.While non metabolic acidosis were 239 cases (81.25％),and 136 cases were combined with myocardial lesion.Therefore,the myocardial lesion was significant correlation with metabolic acidosis (P ＜ 0.01).Iron deficiency anemia was 123 cases and combined with 15 cases (12.19％) convulsion,while the convulsion prevalence rates of non iron deficiency anemia was 6 cases (3.65 ％).There was a statistically significant difference between the convulsion prevalence rates of iron deficiency anemia and that of non iron deficiency anemia (P ＜ 0.01).Conclusion Rotavirus diarrhea can lead to extraintestinal organe damage,and the clinical doctors should pay attention to them.

Objective To search for the methods of inducing the high stability rat middle cerebral artery occlusion (MCAO) model and to noninvasively assess the method of model effect. Methods Six kinds of filaments with different diameters were used to induce rat MCAO models and their success rate, incidence of subarachnoid hemorrhage, and infarct volume were compared. The model scores were performed to assess the model effect after the operation and at 24 hours after reperfusion. Results Higher model success rate and lower incidence of subarachnoid hemorrhage were achieved when the 0. 28 mm in diameter and 0.32-0.34 mm in tip diameter filaments were used. 1he sensibility and specificity were higher when the model scores were performed at 24 hours after reperfusion. Conclusions Using the filaments of 0.28 mm in diameter and 0. 32-0. 34 mm in tip diameter for intraluminal thread could improved the stability of the models. The model scores at 24 hours after reperfusion could noninvasively assess the model effect.

Objective To investigate the feasibility and efficacy of vaginal sacrospinous ligament fixation (VSSLF) procedure which reserving uterus in the treatment of uterine prolapse. Methods 8 cases with uterine pro-lapse underwent VSSLF procedures which reserving uterus from July 2006 to December 2008. Those with incontinence d urine simultaneously underwent ventrofixation of the middle piece of the urethra and folding of the ligamentum pos-terius of the uretha and those with anterior or posterior vaginal wall prolapse underwent eolporrhaphia anterior-posteri-or. Results All patients successfully accomplished by VSSLF,without intraoperative or postoperative hemorrhage or organ injury. All patients' prolapse symptoms were relieved efficently according to the pelvic organs prolapse quantifi-cation(POP-Q). Patients were followed-up for 18 months after operation. 8 patients all recovered well without any complication related to the procedure after operation. Conclusion VSSLF is a simple,safe,effective and micro-inva-sire way for patients with uterine prolapse who want to reserve uterus.