The immune mechanisms of food allergy relate to allergen and immunologic response.Different kind of allergens and different immunologic response will impact on the clinical features,diagnoses and management of food allergy.It is benefiting for clinical practice to insight the immune mechanisms of food allergy.

Objective To analyze the reaction in rabbit's esophagus after inserting metallic nitinal stent and obtaining suggestion for the clinical application.Methods The selfexpanding nitinal stent was placed in each of eight rabbits's esophagus. The rabbits were killed at 2,4,6,8 weeks after implantation. Tissue around the stent was investigated with naked eye and microscopy. Results Chronic inflammation and edema were seen in the submucous tissue 2 week after stenting. Slight stenosis occurred at the regions of two ends of the stent and scattered vegetations like polyp were seen with naked eye at 4 weeks, with inflammatory cell infiltration, partial mucous polypoid hyperplasia under microscopy. The pathological changes were more obvious at 6 weeks comparing with the 4 weeks. The lower end of one stent was embedded in esophageal wall. Two rabbits of 8 weeks' group died of obstruction with food tarried inside the stents.Conclusions The restenosis after stenting were mainly seen at the region of terminals of stent. Thus reformation of stent should be taken place urgently especially at the terminals for the prevention of restenosis.

BACKGROUND:An appropriate biomaterial can be directly combined with autologous or al ogeneic skin cel s to construct tissue-engineered skin, which can accelerate skin repair after transplantation onto the skin wounds. It is a good idea to solve the deficiency in skin sources. OBJECTIVE:To study the biological characteristics of the complex with bone marrow mesenchymal stem cel s and to investigate the application of this complex in skin defect repair. METHODS:Twenty-eight Sprague-Dawley rats, SPF grade, were randomized into two groups (n=14 per group). By adjustment of temperature, time, pressure and area of NC perm instrument, scald models were made in rats. Rats in the treatment group were given the repair using composite bone marrow mesenchymal stem cel s, while those in the control group were given vaseline gauze repair. Repair effects were compared between two groups. RESULTS AND CONCLUSION:(1) After 72 hours of culture, the composite bone marrow mesenchymal stem cel s smal with round shape distributed dispersedly. After 5 days of culture, the cel s began to stretch and the cel morphology became unstable. After three passages, the cel morphology became stable. The results of antigen identification showed the expression of CD44 and CD29 but the low expression of CD45 and CD34 in bone marrow mesenchymal stem cel s. (2) Twenty-eight days after repair, there was no obvious scar on the wound surface of the treatment group, but a little shrinkage and obvious scar stil existed in the control group. Moreover, in the control group, the epidermal layer of the skin was relatively thick, and the connection between the basal layer and the dermis was unsatisfactory. In the treatment group, obvious epidermal cel stratification, neat arrangement, and tight connection between the epidermis and dermis were observed. In summary, bone marrow mesenchymal stem cel s are a special class of cel s that have pluripotent ability and are more readily available. These cel s are the preferred target cel s for skin defect repair to promote early healing of the skin and improve blood circulation defect site, which are confirmed to have high clinical value.

BACKGROUND: In recent years, acel ular human cadaveric dermis has been applied for repairing dermal defects and fil ing soft tissues, but this repair material is expensive and difficult to obtain. OBJECTIVE: To explore the repair effect of tissue-engineered acel ular dermis on wound healing of skin ulcer. METHODS: Sprague-Dawley rats were randomly divided into autologous reticular dermis and acel ular dermis groups. The tissue-engineered acel ular dermis was prepared by hypotonic solution, 2.5 g/L trypsin, 0.5% Triton X-100 and PBS solution, and in the meanwhile, a rat model of skin ulcer was established. Then, autologous skin graft and tissue-engineered acel ular dermis were transplanted into the autologous reticular dermis group and acel ular dermal group, respectively. Subsequently, comparative study about the repair effect and relative mechanism between two groups was performed. RESULTS AND CONCLUSION: The tissue-engineered acel ular dermis showed a white and grainy shape at room temperature. And hematoxylin-eosin staining showed that: the internal structure of tissue-engineered acel ular dermis exhibited a dendritic distribution, and the gap between col agen fiber bundles was relatively large. At 2, 3, 4 and 8 weeks after dermal transplantation, the survival rate of skin graft in the acel ular dermis group was significantly higher than that in the autologous reticular dermis group (P < 0.05). And the wound contraction rate in the acel ular dermis group was significantly higher than that of the autologous reticular dermis group at 3, 4 and 8 weeks after dermal transplantation (P < 0.05). In addition, the average diameter and clearance rate of col agen fibers in the normal skin around wound in the acel ular dermis group were significantly higher than those of autologous reticular dermis group (P < 0.05). To conclude, it is relatively simple to prepare tissue-engineered acel ular dermis under mild conditions, and the prepared tissue-engineered acel ular dermis presents a regular shape. Moreover, this acel ular dermis achieves desired outcomes in repairing dermal defects, which can promote wound healing by reducing the intradermal DNA residual and significantly improving some biomechanical properties in vivo.

Objective To study the status of knowledge-attitude-practice (KAP) of environmental health among urban and rural residents and the influencing factors. Methods Totally,4 607 urban and rural residents from Lanzhou,Taiyuan,Shanghai and Qingdao were recruited and investigated by the self-designed questionnaire from July 3rd to August 10th 2007. In the questionnaire,a certain number of related questions were designed mainly on environmental health knowledge,attitude and practice. The datas were analyzed with SPSS13.0 software. Results The average scores of environmental health knowledge,attitude and practice of urban and rural residents were 4.14?1.30,4.88?0.85 and 4.05?1.11 respectively. The main influencing factors of environmental health knowledge were education background (?=0.220,P

Objective To understand the indoor air pollution in Beijing. Methods Based on the data from epi-demiological investigation and air pollution monitoring, the investigation was carried out by choosing 3 districts in Beijing including Dongcheng, Shijingshan and Haidian during Jan-Mar in 2000. 70 houses were selected in each district randomly by cluster sampling, the indoor concentrations of PM10, PM2.5 and SO2 in bedroom and kitchen were monitored. The data on concentrations of PM10, PM2.5 and SO2, and the environmental conditions of each sampling place were all recorded in the unified questionaires. Results The total average concentrations of pollutants in indoor air of 3 districts were 0. 555 mg/m3 for PM10 and 0. 512 mg/m3 for PM2.5 respectively. The total average concentration of 0.052 mg/ m3 for SO2 was below the ralated National Stardard. The concentrations of PM10 and PM2.5 in the bedrooms and kitchens in each district all exceeded the related national standards, and revealed no significant differences between bedrooms and kitchens. The SO2 levels in kitchens in Dongcheng District and Haidian District were significantly higher than those in bedrooms(P

Intervertebral disc degeneration (IVDD) takes great affection on people health all over the world.With the extension of human life and aging,the number of sick people will grow rapidly.At present,both conservative and surgical treatment can only alleviate the symptoms,rather than the fundamental treatment for the process of degeneration.Therefore,the application of cell therapy in the treatment of degenerative disc regeneration is an innovative treatment.However,it's a great bottleneck for exogenous transplanted stem cells to survive in degenerated intervertebral disc with harsh environment containing strong force,acidic pH,hypoxia,hyperosmolarity and limited nutrition.In recent years,tissue endogenous repair has been demonstrated to be present in almost all human tissues,and endogenous stem cell play an important role in the process.Similarly,nucleus pulposus was also confirmed the existence of such a kind of tissue specific stem cells-nucleus pulposus derived stem cells (NPSCs)-a new promising candidate for the treatment of IVDD.NPSCs,as a new kind of stem cells,hold either the characteristic of stem cell and the special potential of themselves,such as the nice adaption of the environment in intervertebral disc and the superior potential of cartilage differentiation.Nonetheless,the isolations of NPSCs were different;cell phenotypes were lack of wildly acceptable standard and the biological characteristics were various among different NP sources.In addition,whether the application of NPSCs could be used in nucleus pulposus tissue engineering still calls for further research.Due to the unclear in degeneration mechanism of IVDD,the study on degeneration mechanism of NPSCs may play a great important role in clarifying this issue.

Objective To investigate the expressions and correlation of monocarboxylate transporter-1 (MCT1) and CD147 in human gliomas,and analyzee the relationship between their expressions and prognosis.Methods Immunohistochemistry SP methods were applied to detect the expressions of MCT1 and CD147 in 28 cases of low grade gliomas and 32 cases high grade gliomas.Their expression level and correlation were analyzed statistically.Prospective cohorts were set to acquire follow-up data.Kaplan-Meier curve and Cox multi-factor model were used to analyze patients’ prognosis.Results Both CD147 and MCT1 had strong expressions in human gliomas.The positive expression rate of MCT1 and CD147 (93.8% and 90.6%) and the strong positive expression rate (73.3% and 72.4%) in high grade gliomas were significantly enhanced compared with that of low grade gliomas (positive expression rate:46.4% and 50%,strong positive expression rate:30.8% and 35.7%,P

Objective To evaluate the method and result of thyroid artery embolization as a new therapy for Graves disease.Methods Ten patients with Graves disease underwent selective thyroid artery embolization. Totally 25 thyroid arteries were embolized with PVA microspheres. The indications to this therapy were as following: Graves disease with recurrent clinical symptoms or with leucopenia during the period of treating with administration of antithyroid drugs or recurrence after subtotal thyroidectomy. Results Serum level of thyroid hormones dropped significantly [median FT 3 from 20.90pmol/L(13.36～50.92pmol/L) to 7.81pmol/L(3.67～35.3pmol/L), median FT 4 from 57.9pmol/L(30.96～57.9pmol/L) to 28.13pmol/L(20.44～39.60pmol/L), ( P

Aim To study the effect of saikogenin d (SGD) on prostaglandin E 2(PGE 2) production in C 6 rat glioma cells. Methods Radioimmunoassay was applied to determine PGE 2 production in the cells. Scintillation counting was used to measure liberation of -arachidonic acid (AA) from the cells labeled with -AA. Results In vitro, SGD alone at 1～20 ?mol?L -1 did not affect PGE 2 release from cells, but inhibited its release induced by A23187, a Ca 2+ ionophore. The inhibition was concentration-dependent, with the IC 50 value of about 3 ?mol?L -1. SGD (2～10 ?mol?L -1 ) had no inhibitory effect on A23187-induced AA release or on the conversion of AA to PGE 2 in microsomal preparations. Conclusion SGD inhibites A23187-induced PGE 2 production in C 6 rat glioma cells in vitro, without either inhibition of free AA liberation or a direct inhibition of cyclooxygenase (COX) activity.