A novel fingerprint system of Chinese medicinal materials employing both DNA and chemical means has been proposed. To establish the fingerprint system is very effective to the quality control of Chinese medicinal materials, because it will be controlled from chemical substance and genetic information. The chemical components and DNA fingerprints will show the complex chemical components and biological source, respectively. DNA fingerprint could give a further proof to distinguish Chinese materia medica from the chemical. The combination of chemical and DNA fingerprints is constructive to quality control of Chinese materia medica, the cultivar identification and the selective breed of good varieties for medicinal use. To carry out the fingerprint system of Chinese medicinal materials, it is necessary to establish fingerprint database of Chinese medicinal materials, including chemical and DNA fingerprint, as well as many detection methods, and the pattern recognition system by computer is also necessary.

Objective To explore the relationship between ischemia/reperfusion (IR) and the expression ET-3 and GFAP. Methods Thirty-six adult male mice were randomly divided into three groups: ⑴ ischemia/reperfusion group(IR,n=24), bilateral common carotid arteries(BCCA) of mice were ligated for 7 minutes and reperfused for 1day,3day,5day and 10day; ⑵ a sham operation group (SO, n=6); ⑶ normal control group(NG, n=6). The ET-3 and GFAP expressions in the layers Ⅲ-Ⅵ of frontal and parietal lobes of mice in the three groups was detected by immunohistochemisty. Results The expressions of ET-3 and GFAP in the layers Ⅲ-Ⅵ of frontal and parietal lobes significantly increased in I/R group(P

Objective To investigate the distribution characteristics of inhalant and ingestive allergens in patients with allergic rhinitis(AR)in Lishui area,and then provide evidences for the clinical diagnosis,treatment and prevention of AR.Methods The detection data of serum sIgE antibodies to allergens from 1 610 patients with suspected AR visited the Department of Otolaryngology of Lishui Central Hospital during January 2020 and December 2022 were collected,and the distribution characteristics of various allergen spectra in AR patients were further analyzed.Results A total of 1 020 patients were diagnosed with AR and the positive rate of serum sIgE an-tibodies to allergens was 63.35%.The positive rate(66.59%)in male AR patients was significantly higher than that in females(59.48%,P＜0.01).The main allergen among AR patients in Lishui area was house dust mite.The top five inhalant allergens were house dust mite(77.94%),mixed mold(10.20%),cat dander(9.22%),tree pollen(5.88%),and dog dander(3.43%).The top five ingestive allergens were milk(23.33%),crab(7.75%),cashew nut(6.67%),shrimp(4.41%),and egg protein(3.33%).The positive rates of serum sIgE antibodies to milk,crab,and shrimp in males were significantly higher than that in females(P＜0.05).There were significant differences in the positive rates of serum sIgE antibodies to house dust mite,mulberry tree,cockroach,tree pol-len,and grass pollen among different age groups(P＜0.05).The positive rates of serum sIgE antibodies to egg protein,milk,crab,and amaranth were significantly different(P＜0.05).There were seasonal differences in the positive rates of serum sIgE antibodies to house dust mite,mulberry,mixed mold,tree pollen,and grass pollen(P＜0.05).Conclusion The allergens of AR patients in Lishui area are diverse and complex,and are affected by factors such as gender,age and season.

Objective To evaluate the value of ultrasound elastography combined with serum markers in the assessment of chronic hepatitis B fibrosis. Methods 100 patients with chronic hepatitis B liver fibrosis who were diagnosed and treated in our hospital from January 2015 to December 2017 were randomly selected. All patients were given hepatic histopathological tests, serological tests, and ultrasound elastography. The liver biopsy results were taken as the final diagnosis results. The accuracy of classification of hepatitis B fibrosis by the combined diagnosis of ultrasound elastography and serum markers was observed. Results The total accuracy of the combined examination was 91.00％ with histological examination as the final results. There was no statistically significant difference between the results of the two examinations (P>0.05). The ultrasound elastography score and FIB4 score in the patients with hepatitis B fibrosis grade F1-F4 were increased along with the increase of grade, and there was statistically significant difference (P<0.05). Conclusion Ultrasound elastography combined with serum markers has an important clinical value in the assessment of the degree of chronic hepatitis B fibrosis. It is non-invasive, easy to operate and highly accurate, and can provide a reliable basis for disease diagnosis and staging.

Because of the unsatisfactory treatment options for breast cancer (BC), there is a need to develop novel therapeutic approaches for this malignancy. One such strategy is chemotherapy using non-toxic dietary substances and botanical products. Studies have shown that Panduratin A (PA) possesses many health benefits, including anti-inflammatory, anti-bacterial, anti-oxidant and anti-cancer activities. In the present study, we provide evidence that PA treatment of MCF-7 BC cells resulted in a time- and dose-dependent inhibition of cell growth with an IC50 of 15 µM and no to little effect on normal human MCF-10A breast cells. To define the mechanism of these anti-proliferative effects of PA, we determined its effect critical molecular events known to regulate the cell cycle and apoptotic machinery. Immunofluorescence and flow cytometric analysis of Annexin V-FITC staining provided evidence for the induction of apoptosis. PA treatment of BC cells resulted in increased activity/expression of mitochondrial cytochrome C, caspases 7, 8 and 9 with a significant increase in the Bax:Bcl-2 ratio, suggesting the involvement of a mitochondrial-dependent apoptotic pathway. Furthermore, cell cycle analysis using flow cytometry showed that PA treatment of cells resulted in G0/G1 arrest in a dose-dependent manner. Immunoblot analysis data revealed that, in MCF-7 cell lines, PA treatment resulted in the dose-dependent (i) induction of p21WAF1/Cip1 and p27Kip1, (ii) downregulation of Cyclin dependent kinase (CDK) 4 and (iii) decrease in cyclin D1. These findings suggest that PA may be an effective therapeutic agent against BC.
Apoptosis* ; Breast Neoplasms* ; Breast* ; Caspases ; Cell Cycle Checkpoints* ; Cell Cycle* ; Cell Proliferation* ; Cyclin D1 ; Cyclins ; Cytochromes c ; Down-Regulation ; Drug Therapy ; Flow Cytometry ; Fluorescent Antibody Technique ; Humans ; Inhibitory Concentration 50 ; Insurance Benefits ; MCF-7 Cells ; Phosphotransferases