Objective To investigate expressions of albumin during retinal neovascularization in a murine model of oxygen -in-duced retinopathy.Methods C57BL/6J mice were exposed to (75 ±2)%oxygen for 5 days and returned to room air to induce retinal neovascularization .Retinal neovascularization was observed by fluorescence angiography , and was quantified by counting the endotheli-al nuclei protruding into the vitreous cavity after hematoxylin-eosin staining.The blood-retina barrier was evaluated by Western blot a-nalysis of extravasated albumin in the retina .Results In fluorescence angiograms , irregular neovascularization and fluorescein leakage was observed surrounding the unperfused areas in the hypoxic group .Compared to the normoxic group , there was a significant increase in both-side retinal albumin protein levels in the hypoxic group at P 13, P15 , and P17.Conclusions Albumin expression was in-creased in murine retina under hypoxia , and might be used to detect blood-retinal barrier damage of oxygen-induced retinopathy .

Gastrointestinal stromal tumor (GIST) is originated from the gastrointestinal mesenchymal stem cells,composed of undifferentiated or pluripotent spindle and epithelioid cells,often occurs in the whole range of the gastrointestinal tract and occasionally in the omentum,mesenterium and other areas which are outside of digestive tract.The treatment is difficult due to broad-spectrum biological behaviour of GIST,while surgery may be the only potential method for curing GIST with a risk of recurrence.Currently,there is still not an evaluative standard in the choice of surgery or imatinib therapy as well as the risk of recurrence.The F/NIH consensus,Armed Forces Institute of Pathology (AFIP) standard,modified standard of National Institutes of Health(NIH) and consensus of mathematical model which have been widely used cannot accurately evaluate risk probability of recurrence,so the current researches have focused on the postoperative risk assessment for GIST.In recent years,the nomogram model has been applied to predict the risk of GIST recurrence by some scholars,with the better outcomes.

Long-term potentiation(LTP)is an important form of synaptic plasticity and an objective indicator to investigate learing and memory synaptic mechanisms.With the development of brain slice technology,more and more experiments associated with LTP are carried out on brain slices,which aim to investigate the mechanism in biology and the change in physiology or biochemistry are carried out on the brain slice.This paper gives an overview of recent advances in research of LTP with technology of brain sliceby suchexamples as follows:The regulated expression mechanisms of long-term potentiation at CA1 synapses,the characteristics of LTP induced in hippcampal slices and its relation with the slice-recovery conditions,the enhancement of the magnitude of early longterm potentiation at CA1 hippocampal synapse by the activation of dopamine receptor,and the enhancement of associative long-term potentiation by the activation of β-adrenergic receptors at CA1 synapses in rat hippocampol slices.

Objective To investigate the effect of fast-aging on the excitability of hippocampus CA1 neurons in mouse and the possible interaction between fast-aging and hippocampus. Methods Using brain slice and extracellular recording technique to record the firing of hippocampus CA1 neurons in fast-aging(SA M-P/8) and normal control mice, after preprocessing, neural firing train were obtained. Using neural firing rate and inter-spike-inter to investigate interaction between fast-aging and hippocampal neural firing. Results The neural firing rate of hippocampus CA1 neurons in fast-aging mice is (1.052±0.364) Hz(n=14), while the neural firing ratein normal control mice is (4.416±1.306) Hz(n=22). In fast-aging mice, 80.5% inter spike intervel(ISI) is longerthan 1sec, but in normal control mice, 95.6% ISI is shorter than 0.5sec. Conclusion The decreased firing rate of hippocampus CA1 and longer ISI observed in the fast-aging mice indicates that fast-aging significantly inhibit hippocampal CA1 neurons excitability.

Objective To explore a rapid method for classification of microorganisms.Methods The electrophorese fingerprinting, direct sequence of 16S rDNA and 16S-23S rDNA ISR after PCR, multiplex PCR for 16S rDNA and antibiotic resistance genes, were utilized to explore fast approaches of extracting total DNA from different clinical specimens.Results The specific 16S-23S rDNA ISR fingerprinting fragments were shown on the genus or species level in bacteria and fungi.So fingerprinting can be used to identify pathogenic microorganisms, to differentiate the evolution relations or to set the phylogenetic tree by comparing their DNA banding patterns with those of standard strains (NCCLS). Multiplex PCR was able to examine the special genes of genus or species, mecA gene, TEM, SHV and CTX gene in staphylococcus and ESBLs(E.coli or K.pneumoniae) at the same time.Conclusion The part of 16S rDNA sequencing and 16S-23S rDNA ISR genotypes by gel electrophoreses were useful for bacterial species identification in addition, it was clearly more rapid and accurate than culture technique, and the large numbers of strains can easily be examined.Multiplex PCR could provide a good method for identification of microorganisms and analysis of antibiotic resistance at the same time.

OBJECTIVE To survey the knowledge about the prevention by hepatitis B vaccination among vaccine recipients and inquiry persons,and formulate the measure of health education on prevention of HBV. METHODS 349 cases of hepatitis vaccines and inquiry persons in infirmary from Oct 2006 to Feb 2008 were selected,and making questionaire investigation for the understanding of HBV infection. RESULTS The initiative consciousness of prevention to HBV was manily focused on young people. The 20 to 40 years old people were 81%,3% below 20 years and 16% over 40 years among the investegatees. The correct rate of cognition was 36.4%. CONCLUSIONS To propagandize the knowledge about HBV and make the people to understanding the HVB,and increase the consciousness for prevention. To reduce the mistake cognition for HBV infection and encourage the high-risk people to receive the hepatitis vaccination.

OBJECTIVE To evaluate the methods of improving the air quality in the outpatient departmant area.METHODS The amount of outpatients in different time periods in the morning were calculated in the specialist consulting area;the bacteria in the air collected from the clinic room were collected and cultured in general nutrition agar medium and compared the efficacy for the improvement of air quality between the new and the old methods.RESULTS The air quality of the specialist consulting area was improved significantly through the ventilation and cleaning-floors depending on the amount of outpatients(P

Objective　To study the preventive effect of hepatic metastasis from colorectal cancer and the immune function influence on postoperative patients with colorectal cancer treated by TIL, rIL-2 and cyclophosphamide by portal vein infusion. Methods　One hundred and twenty-four patients with advanced colorectal cancer (Dukes B and Dukes C stage) were randomly divided into immunotherapy group and control group. The control group were treated with 5-Fu and MMC by intravenous injection after resection of colorectal cancer. The immunotherapy group were treated with TIL, rIL-2 and cyclophosphamide by portal vein infusion after resection of the tumor, then with chemotherapy as the control group. All cases were followed-up for 3 years. Results　Eight cases (12.3%) had hepatic metastases from colorectal cancer in the immunotherapy group, but nineteen cases (33.2%) in the control group. There was significant difference between the two groups (P>0.05). The NK, IL-2 activity and CD3, CD4, CD4/CD8 levels in peripheral blood of the immunotherapy group increased significantly after treatment (P<0.05). Conclusions　The TIL, rIL-2 and cyclophosphamide treatment after resection of colorectal cancer is effective in improving antitumorgenic immune function, and preventing hepatic metastases.

Objective:To explore the influencing factors of false-positive serological reaction of syphilis.Methods:A total of 166 patients with false-positive serological reaction of syphilis (false-positive group), 145 patients diagnosed with early syphilis without treatment (positive control group) and 124 persons undergoing entry physical examination (negative control group) were included from January 2017 to February 2020 in Beijing Tongren Hospital, Capital Medical University. The gender, age and underlying diseases of the three groups were compared. Logistic regression was used to analyze the influencing factors of false-positive serological reaction of syphilis. The efficacies of chemiluminescence immunoassay (CLIA) and toluidine red unheated serum test (TRUST) were compared. Paired t test or chi-square test was used for statistical analysis. Results:In the false-positive group (166 cases), the age of 117 cases were more than 50 years old and 49 cases <50 years old. There were significant differences in age ((53.1±13.8) vs (24.7±2.8), t=22.56, P<0.01), autoimmune disease (36.7%(61/166) vs 6.5%(8/124), χ2=35.93, P<0.01), hepatitis (9.6%(16/166) vs 3.2%(4/124), χ2=4.92, P=0.026) and tumor (6.6%(11/166) vs 0.8%(1/124), χ2=4.68, P=0.030) between the false-positive group and the negative control group. There were significant differences in gender (there were 91(54.8%) males and 75(45.2%) females in the false-positive group, and 103(71.0%) males and 42(29.0%) females in the positive control group, χ2=8.67, P=0.003), age ((53.1±13.8) vs (34.4±12.9), t=20.13, P<0.01) and autoimmune disease (36.7%(61/166) vs 6.9%(10/145), χ2=39.14, P<0.01) between the false-positive group and the positive control group. Multivariate logistic regression analysis showed that gender (odds ratio ( OR)=2.692, 95% confidence interval ( CI) 1.504-4.816, P=0.001), age ≥50 years old ( OR=30.512, 95% CI 15.959-58.335, P<0.01), autoimmune disease ( OR=2.677, 95% CI 1.258-5.695, P=0.011) and hepatitis ( OR=4.408, 95% CI 1.799-10.799, P=0.001) were the influencing factors of false-positive serological reaction of syphilis. In the false-positive group, the positive rate of TRUST was 84.9% (141/166), which was higher than that of CLIA (23.5%(39/166)). The difference was statistically significant ( χ2=126.25, P<0.01). CLIA was 1.0-10.0 cut off index (COI) in 36 patients, and >10.0 COI in three patients.The difference was statistically significant ( χ2=52.51, P<0.01). The titers were ≤1∶4 in 139 patients and≥1∶8 in two patients with TRUST positive.The difference was statistically significant ( χ2=262.35, P<0.01). The sensitivity and specificity of CLIA were 95.2% and 96.0%, respectively, and those of TRUST were 77.2% and 91.1%, respectively. Conclusions:The influencing factors of false-positive serological reaction of syphilis include patients age ≥50 years and with autoimmune disease or hepatitis. The false-positive rate of TRUST is significantly higher than CLIA.

Objective To investigate the situation of drug resistance and nosocomial infection of multi-drug resistant bacteria (MDR),guidance for clinical rational use of antibiotics.Methods A total of 1521 strains of MDR was isolated from January 2015 to December in Beijing Tongren Hospital,using matrixassisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) identification of bacteria,VITEK-2 Compact and Kirby Bauer (KB) method for drug sensitivity test.Results In 1 521 strains of MDR,Acinetobacter Baumanii were 589 strains (38.7％),nosocomial infection rate were 16.6％;350 strains of Escherichia coli (23.0％),nosocomial infection rate were 9.0％;249 strains of Staphylococcus aureus (16.4％),nosocomial infection rate were 2.7％;171 strains of Klebsiella pneumoniae (11.2％),nosocomial infection rate were 14.3％;150 strains of pseudomonas aeruginosa (9.9％),nosocomial infection rate were 64.7％;12 strains of Enterococcus faecium (0.8％),nosocomial infection rate were 16.7％.MDR Acinetobacter Baumanii,MDR Pseudomonas aeruginosa,extended-spectrumβ-lactamase (ESBL) + Escherichia coli and ESBL + Klebsiella pneumoniae resistance rate to Imipenem were 100％,91.5％,0.6％ and 55.6％.Conclusions MDR pseudomonas aeruginosa (MDR-PAE),MDR acinetobacter baumanii (MDR-AB) and ESBL + Klebsiella pneumoniae were highly resistant,and the nosocomial infection rate were higher.