To understand the current situation of public health service system by using diect network report,system report and questionaire surey, so as to provide reasomable suggestion for the existing problems.

Traditional Chinese medicine (TCM) with the characteristics of holistic view and treatment based on syndrome differentiation, has rich clinical experience thousands of years and demonstrates promising effects to cure complex disease. However, due to the features of multi-component, multi-target and synergistic effect existed in TCM, the effective substances and mechanisms of action are not clear, the qualities of TCM are out of control, and scientific and correct assess system is waiting to be established. The network pharmacology is a novel subject based on the construction of multi-layer networks of disease-phenotype-gene-drug to predict the drug targets in a holistic view, and promote efficiency of drug discovery. Methodologically, network pharmacology integrated the notions of comprehensive research and systematic assessment which agree with the characteristics of holistic view and treatment based on syndrome differentiation in Chinese medicine. Our paper reviewed the challenge and chance within the modernization of TCM, the concept and technology of network pharmacology, and its preliminary application in investigation of TCM. The theoretical system of network pharmacology is emphasized, and the potential prospect of its application in modernization in TCM is focused.

Congenital disorders of glycosylation(CDG) are a rapidly growing group of genetic diseases that are due to defects in the synthesis of glycans and in the attachment of glycans to other compounds. Most CDG are multisystemic diseases often involving severe psychomotor retardation. The CDG causing sialic acid deficiency of N-glycans can be diagnosed by isoelectric focusing of serum transferrin. Glycan structural analysis, yeast genetics and knockout animal models are essential tools in the elucidation of novel CDG. In this review, we focus on the current knowledge of the pathogenesis and identification of nine primary glycosylation diseases.

The distribution of afferent terminals from VL nucleus to cruciate cortex was studied by using nerve fibers degeneration and electron microscopic technigues. Cortical area 4 could be further divided into three subdivisions according to their geographical relations to the eruciate sulcus: an anterior, a posterior and a lateral zone, respectively. It was demonstrated that the degenerating axon terminals were mainly distributed in anterior and lateral zones. Much less terminals were observed in the posterior zone. Totally 112 terminals were observed in the anterior zone. Among them, 78.6％ were seen in layer Ⅲ, 19.6％ in layer Ⅰ, 1.8％ in layer Ⅴ. Of the 112 terminals found in the lateral zone, 62.5％ were distributed in layer Ⅲ, 31.2％ in layer Ⅰ and 6.3％ in layer Ⅴ. On the other hand, only 33 degenerating terminals were found in posterior zone. their distributions were: 51.6％ in layer Ⅰ, 42.4％ in layer Ⅲ and 6.0％ in layer V. Approximately 75％ of the degenerating axon terminals examined in these three zones made synaptie contacts with dendritic spines, and 25％ with dendritic shafts. The vast majority of these synapses could be classified as asymmetrical type synapse.In area 6, most of the degenerating terminals were encountered in layer Ⅰ and Ⅲ. All of them formed asymmetrical type synapses with dendritic spines. No degenerating terminals could be found in area 3a.

Synaptic connections of commissural axon terminals from contralateral motor cortex have been studied by using anterograde degeneration and electron microscopy in the cortical area 4? of cat. The results showed that most of the commissural terminals display electron-dense type degeneration, and 92 degenerating terminals were identified in area 4?. The number of terminals in layer Ⅰ was nearly equal to layer Ⅲ. In layer Ⅲ, an asymmetrical synapse formed between a degenerating terminal and the soma of a bipolar cell was observed. In layers Ⅴ-Ⅵ, no degenerating terminal could be found. Among the total degenerating terminals, 77.2% were found to make synapses with dendritic spines, 21.7% made synapses with dendritic shafts and 1.1% with soma, and all of these synapses were of the assymmetrical type.

Objective:To compare the effects of levosimendan injection at single dose and multiple doses on the left ventricular function , biomarkers and neuroho-rmonal activity in the patients with acute exacerbation of advanced heart failure .Methods:Totally 39 patients with chronic heart failure at acute exacerbation were divided into two groups , single dose group and multiple doses group .All the patients were given loading dose of 6μg· kg-1 levosimendan at baseline , and then given 0.1μg· kg-1 · min-1 continuous 24h in-fusion pump.The above regimen was administered once again after one month and six months in the patients of multiple doses group . Left ventricular end systolic volume ( LVESV ) , left ventricular end diastolic volume ( LVEDV ) , left ventricular ejection fraction (LVEF), mitral annulus systolic velocity (SM), myocardial performance index (MOI), blood brain natriuretic peptide (NT-proB-NP), tumor necrosis factor alpha (TNF-α) and interleukin 6(IL-6) were compared between the two groups before the treatment , and after tge three-day and 6-month treatment.Results:Compared with those before the treatment , LVESF, LVEF, Sm, MPI, NT-proB-NP, IL-6 and TNF-αin in the two groups were significantly improved after the three-day treatment (P<0.05).The indices were sig-nificantly improved in multiple doses group after the 6-month treatment when compared with those before the treatment and in single dose group, and the differences were statistically significant (P<0.05).Conclusion: Repeated intravenous administration of levosi-mendan is superior to the single dose administration in improving hemodynamics and inflammatory factors .

ObjectiveTo compare the effect of salt bag compression and arterial compressor haemostat for patients after cerebral angiography.Methods Four hundred and ninety-five patients undergoing cerebral angiography were divided into two groups according to the methods of arterial compression:salt bag compression group (243 cases) and arterial compressor haemostat group (252 cases) in which 131 patients used the YM-GU-1229 arterial compressor haemostat and 121 patients used the NZ-1 arterial compressor haemostat.The associated complications within 3 days were recorded.ResultsThe incidence of complications in salt bag compression group was 3.70％(9/243),in arterial compressor haemostat group was 9.92％ (25/252),and there was significant difference between the two groups(P ＜ 0.01 ).The incidence of complications in YM-GU-1229 arterial compressor haemostat was 12.21％ ( 16/131 ),in NZ-1 arterial compressor haemostat was 7.44％(9/121 ),and there was no significant difference between the two arterial compressor haemostats (P ＞ 0.05).ConclusionThe incidence of complications of arterial compressor haemostat is higher than that of salt bag compression.

Objective To observe whether the bone marrow stromal cells (BMSCs) which carry BMP-2/Tet-on genes and are microcapsulated by alginate polylysine alginate (APA) and seeded onto calcium phosphate cement (CPC) scaffolds can express bone morphogenetic proteins-2(BMP-2) and secrete collagen proteins.Methods The BMSCs from rats were prepared,transfected with adenoviruses carrying BMP-2/Tet-on genes,and then microcapsulated by APA.Eight porous CPC scaffolds were prepared and mounted onto a 12-hole plate.The experiment was conducted in 3 groups,with 4 parallel specimens in each group.Microcapsulated BMSCs with the target gene were seeded onto the CPC scaffolds in the experimental group; the transfected BMSCs were seeded into the plate holes as positive controls; APA microcapsules without cells were seeded onto the CPC scaffolds as blank controls.The culture medium in each group was taken for ELISA examination on 3,6,9 and 12 days to evaluate the BMP-2 concentrations respectively.The samples in the experimental group were fixed,sliced and dyed to observe secretion of collagen.Results On 3,6,9 and 12 days after culture,the BMP-2 concentrations were 4713.98 ± 178.50 μg/mL,3288.85 ± 194.38 μg/mL,1292.25 ± 300.11 μg/mL and 337.19 ± 84.49 μg/mL respectively in the experimental group; 4663.87 ±242.99 μg/mL,3250.67 ±293.72 μg/mL,1276.74 ± 157.10 μg/mL and 293.65 ±92.48 μg/mL respectively in the positive controls; 105.14 ± 10.93 μg/mL,91.42 ± 18.00 μg/mL,89.63 ± 12.99 μg/mLand 108.72 ± 23.90 μg/mL respectively in the blank controls.There were significant differences between the 3 groups at each same time point (P ＜ 0.05).The blank controls were significantly different from both the experimental group and the positive controls (P ＜ 0.05),but there were no significant differences between the experimental group and the positive controls (P ＞ 0.05).Collagen fibrils were observed by optic microscopy in the sections of the experimental group.Conclusion As microcapsulated BMSCs can survive in CPC scaffolds and express a significant amount of BMP-2 and secrete collagen proteins as expected,they can be used for further experiments in vivo.

Aniline Compounds ; poisoning ; Humans ; Poisoning ; therapy

MOS 36-Item Short-Form Health Survey has been widely recognized, but the application in the field of traditional Chinese medicine rheumatism was less. This article aimed to summarize the application of MOS 36-Item Short-Form Health Survey in Chinese medicine for rheumatism. This may provide certain reference for the future application.