Objective By systematically analyse from 1950 to 2013 Chinese medicine nursing research situation,the paper aimed to give a comprehensive insight into the current situation of the development in Traditional Chinese Medicine (TCM) nursing,speed up the nursing literature resources development and utilization of traditional Chinese medicine and promote the comprehensive development of the discipline.Methods Using China National Knowledge Infrastructure (CNKI) as the data resource,the method of literature metrology was adopted to retrieve the valid papers,including published time distribution,regional distribution,the dispatch of productive organization,issuing system of institution,different number distribution of authors,the author fund of cooperative research situation,the papers published periodical distribution,the cited frequency content.Results A total of 3 569 effective papers in this study were selected,of which papers from 2010 to 2013 accounted for 46.70％.19 institutions had the highest paper production,dispatch agencies belonged to different systems,among whichhospital and school accounted for a total amount of 96.30％,the core authors were 38 in the discipline research field,they published a total of 194 articles,but the two literature metrology index analysis of cooperation degreeand co-author rate showed that the authors lacked overall cooperation in the field of research.Conclusions Although the core authors had formed in the field of study,but through the analysis of parrot card law,the subject has not yet to reach maturity that the subject of the author's ideal distribution state.In scientific research activities for the future,researchers should strengthen cooperation,seek more high-level papers funded in order to enhance the development of TCM nursing level.Nurse practitioners of traditional Chinese medicine and related scientific research personnel should pay attention to related academic papers from areas prone to paper and paper productive organization.Relevant academic journals,especially the core journals in the field of research should increase Chinese medicine nursing papers from low level hospitals and basic medical service organization.At the same time we look forward to professional nursing journal of traditional Chinese medicine being founded.

Objective To evaluate the damaging degree of hepatic cell in the patients with hepatocirrhosis. Methods 30 cases of hepatocirrhosis and 15 cases of normal controls were performed the dynamic imaging with 99mTc-EHIDA. By the three compartmental model configurations, we acquired the time-activity curers of the liver cell,and calculated the function indexes liver cell extraction and excretion. Results The peak time and mean residence time in the hepatocirrhosis groups were higher than those in the normal controls(P

Objective To investigate the serum concentration of serum Fas(sFas) in patients with Graves' disease(GD) before and after ~(131)I therapy.Methods Serum levels of sFas were measured with enzyme linked-immuno-sorbent assay(ELISA) in 61 patients before and after ~(131)I therapy,and the results were compared with that the control subjects.Results The concentration of sFas was significantly higher in GD patients compared with that in the control subjects[(174.8?94.6)ng/L vs (96.6?10.2)ng/L,P0.05],and was significantly lower in patients with euthyroidism after 131 I therapy[(111.2?13.2)ng/L,P

Immunity reconstruction treatment using dendritic cells-cytokine induced killer cells (DC-CIK)not only is effective in killing cancer cells, but also enables a quick recovery of chemotherapy patients, thus has received more and more attention for its clinical use. Radix astragali polysaccharide, the extract of traditional Chinese medicine radix astragali, not only has an anti-tumor function, but also can enhance the immunity of cancer patients and reduce the side effects of the chemotherapy. Further researches are needed to validate the combined use of these two treatments in alleviating clinical symptoms of post-chemotherapy breast cancer patients.

Objective To investigate the effects of the transfection of DNMT1 antisense oligonucleotide wrapped in liposome on the promotor methylation and expression of progesterone receptor in leukemia cell lines so as to find a new pathway of methylation for gene therapy. Methods Artificially synthesized DNMT1 antisense oligonucleotide MG88 wrapped in liposome was transfected into the leukemia cell line. Changes of DNMT1 & PRB mRNA were detected by RT PCR, and the changes of the methylation status of PRA & PRB were analyzed by MSP. Results After transfection of leukemia cell line with MG88, DNMT1 mRNA decreased significantly. PRA & PRB promotors were demethylated, and the expressions of PRB mRNA increased significantly. Conclusion MG88 can induce the demethylation of PR promoters in leukemia cell line, resulting in the increase of PRB mRNA expression. So MG88 can be used in the experimental study of DNA methylation for gene therapy.

OBJECTIVE:To investigate the effects of multidrug resistance gene 1(MDR1)C3435T polymorphism on the dose of analgesia agents(dezocine combined with sufentanil)after joint replacement. METHODS:300 patients receiving joint replace-ment were selected from Tianjin People's Hospital and Tianjin Port Hospital during Jan. 2014-Feb. 2016. They were given dezocine and sufentanil for postoperative analgesia. PCR-RFLP was used to determine MDR1 C3435T polymorphism;VAS scores,Ramesy scores,the dose of dezocine+sufentanil and the occurrence of ADR were compared among different genotypes. RESULTS:Among 300 patients,there were 100(33.3%),102(34.0%)and 98(32.7%)cases of MDR1 C3435T CC,CT and TT genotype,respec-tively,the frequencies of which were all in line with Hardy-Weinberg balance (P>0.05). There was no statistical significance in VAS scores and Ramesy scores among different genotypes 0,24,48 h after surgery(P>0.05). No excessive sedation was found. The dose of dezocine+sufentanil in CT and TT genotype were all significantly lower than CC genotype 0-24 h,>24-48 h after sur-gery,with statistical significance(P<0.05). There was no statistical significance in drug dose between CT and TT genotype during above periods (P>0.05). The incidence of postoperative nausea and vomiting (PONV),ADR in TT genotype were significantly lower than CC and CT genotypes,with statistical significance (P<0.05). There was no statistical significance in the incidence of PONV and ADR between CC genotype and CT genotype,and in the incidence of itch among different genotypes(P>0.05). CON-CLUSIONS:With similar sedation and analgesic effect,MDR1 mutant type have lower resistance to dezocine+sufentanil,and smaller drug dose is need,the incidence of ADR is lower. MDR1 genotype can be regarded as an important indicator of clinical in-dividualized treatment.

Objective To investigate sensitivity and specificity of myositis specific autoantibodies (MSAs) in polymyositis/dematomyositis(PM/DM ) and other neuromuscular disease. Methods Taking serum from 63 patients with PM/DM (PM/DM group)and 60 definite neuromusclar disease(non-myositis) patients(control group). All the sera were detected for two kind autoantibodies:anti-Jo-1,anti-SRP by immunoblotting method. Calculating sensitivity,specificity of anti-Jo-1 and anti-SRP autoantibodies for the diagnosis PM/DM. Results Positive ratio for anti-Jo-1 and anti-SRP autoantidodies were 17% and 5% respectively in the PM/DM group, while none of the sera from control group detected positive.The specificity of both autoantibodies diagnosis for PM/DM were 100% and (95% CI:94%~100%).The sensitivity was 22%( 95%CI:13%～34%). Conclusion Anti-Jo-1 and anti-SRP autoantibodies are highly specific to PM/DM diagnosis.

Objective To explore the relevance among HER-2 gene amplification,microvessel density(MVD),tumor size,lymph node metastasis and histological grading,as well as to discuss the significance of the levels of HER-2 gene amplification and MVD on the prognostic judgment of breast cancer.Method From 2001 to 2005,30 breast cancer specimens were collected from the Affiliated Hospital of Academy of Military Medical Sciences.Coloring in situ hybridization(CISH) was used to examine HER-2 gene amplification in paraffin-embedded tissue samples.Microvessels in tissue were labeled by CD34 antibody using IHC,and the MVD was then counted under optical microscope.Result HER-2 gene amplification was found in 11 of 30 cases(36.7%).MVD in breast cancer samples with HER-2 gene amplification was 67.91?24.36,significantly higher than those samples without HER-2 gene amplification(47.48?13.27,P

Objective To investigate the feasibility of an ileal reservoir with self controllable urination. Methods A segment of ileum was under taken,the middle portion of which was made into a N shaped reservoir and the proximal portion used as the afferent limb.The distal ileum was pulled out and the two skin flaps were taken and encircled the efferent ileum to constitute the urinary outflow.A urinary controller similar to penile clamp was applied to control the urination with a balloon catheter. Results Ten dogs and five patients underwent this procedure and the patients were followed up for 3 to 14 months.Urodynamic examination showed that on the third month,the reservoir in animals reached a maximum capacity of (150?40)ml and a full filling pressure of (24.4?5.3)cmH 2O.The reservoir in the patients reached a maximum capacity of (290?80)ml,a full filling pressure of (36.3?8.2)cmH 2O,a maximum urinary flow rate of (20.3?4.7)ml/s with no residual urine.X ray examination showed good excretory function of the kidney without hydronephrosis.All patients can control urination themselves through the use of the urinary controller. Conclusions The new procedure has the advantages of self control lable of urination,simple surgical performance and few complications.

Objective To investigate the clinical features of anuria caused by pediatric congenital bilateral ureteral stenotic obstruction,and to improve the diagnosis and treatment of the disease.Methods This series included 7 infants(2 males and 5 females;age range,35-57 d) with congenital bilateral ureteral stenotic obstruction.The clinical presentations were characterized by sudden anuria or oliguria.B-ultrasound,cystography and puncture pyelography were performed in all of them,and magnetic resonance urography(MRU) in 3.Based on the definite diagnosis,resection of stenotic segment of the ureter or ureteral implantation was performed on them.Results The diagnosis was confirmed by puncture pyelography in all of the 7 cases.Six cases underwent phase I surgery and recovered.No ureteral stenosis and cystoureteral reflux occurred during a follow-up of 2-4 years.The renal function was normalized in them.One case had stenosis recurrence due to scar formation,and was cured by the second operation.Conclusions Anuria caused by pediatric congenital bilateral ureteral stenosis should be diagnosed and treated as early as possible.Definite diagnosis can be established by puncture pyelography and MRU.Resection of the obstructive segment of the ureter,protection of renal function,and ureteral implantation are the main therapies.