OBJECTIVE:To establish a fingerprint analysis method of Pinellia ternata from the west of Hubei province.METHODS:The samples of Pinellia ternata from different areas harvested at different time and processed by different methods were analyzed by GC method to establish their fingerprint data.RESULTS: The fingerprints of Pinellia ternata have been established using GC data of the chromatographic peaks of five phytosterols from various samples,and these samples had a similarity of between 0.877 and 0.999.CONCLUSION: The fingerprints of phytosterols of Pinellia ternata are stable thus can be used as a new method for the quality control of Pinellia ternata.

Objective: To develop a scale to measure attitudes toward homosexuals in Chinese university students.Methods:Based on surveys and literatures, we conducted the pre-test and test for accessing the construct validity of the scale by EFA ( exploratory factor analysis) and CFA (confirmatory factor analysis) in 1010 Chinese university students.Results: There were three components that could explain 63.84% of the total variance with loading between 0.501 and 0.851 in the scale.The three components could explain 46.67%,10.27% and 6.90% of the variance separately.They were named cognitive tolerance, emotional tolerance and behavior tolerance.The scale had NFI 0.979,NNFI 0.981,CFI 0.985,RMSEA 0.073 by CFA.The Cronbach'?coefficient was 0.931 and the test-retest reliability was 0.839 (with P

Objective:To investigate the impact of self-concealment,self-disclosure,coping style and per- ceived social support on university students'loneliness.Methods:Loneliness and related factors were assessed among 482 university students using scales including UCLA Loneliness Scale,Self-concealment Scale(SCS),Self-disclosure Index(SDI),Simplified Coping Style Questionnaire(SCSQ)and Perceived Social Support Scale(PSSS).Results: The level of university students'loneliness was not high(36.5?7.4);males experienced more loneliness than fe- males(37.4?7.5/35.4?7.3,F=8.25,P0.05). Regression analysis showed that SCS,SCSQ and PSSS predicted UCLA effectively(?=0.207,-0.218,0.157, -0.380).The testing of mediating effect indicated that SCS had direct and indirect impact on UCLA through nega- tive coping style and PSSS;SDI had only indirect impact on UCLA through positive coping style and PSSS.Conclusion:SCS,SDI,SCSQ and PSSS are important factors influencing UCLA,and the intervention of univer- sity students'loneliness should focus on these variables.

Curdlan is a water insoluble exopolysaccharide produced by Alcaligenes faecalis under nitrogen-limiting conditions. After excretion, the polysaccharide is attached the cell wall. Thus enhancement of biomass production during the cell growth phase is important to curdlan production. A strategy of increasing nitrogen source to improve biomass production was adopted for curdlan production by Alcaligenes faecalis (ATCC 31749). In the batch fermentation of curdlan, a relatively higher NH4Cl level of 3.6 g/L with continuous glucose feeding increased the cell density leading to improvement of curdlan production. However, excessive NH4Cl would inhibit curdlan production and biomass production was not improved significantly. In addition, feeding of ammonia water at the initial phase replaced NaOH solution to control pH at 7.0. Subsequently, feeding of NaOH solution was resumed to control pH at 5.6 for curdlan production after ammonia was consumed. As a result, biomass production and curdlan yield were both enhanced remarkably. Feeding of ammonia water during the first 24 h led to biomass production of 18.8 g/L. However, higher cell density did not lead to increase in curdlan production. The maximum curdlan production (72 g/L) was obtained by feeding ammonia water for the first 14 h, during which the cell density was about 11.9 g/L.
Alcaligenes faecalis ; cytology ; metabolism ; Ammonium Chloride ; pharmacology ; Cell Culture Techniques ; methods ; Cell Proliferation ; Fermentation ; beta-Glucans ; metabolism