OBJECTIVETo investigate the feasibility of chondrogenic phenotype differentiation of adult swine bone marrow stem cells(MSCs) in a defined medium as seeding cells in cartilage tissue engineering.

METHODSA volume of 5 ml bone marrow was aspirated from swine iliac crest and cultured in the complete medium of DMEM-LG for two weeks. The growth and ultrastructure of the cultured MSCs were observed. Immunohistochemistry and in situ hybridization were applied to detect the expression of collagen type II.

RESULTSThe MSCs changed from a spindle-like fibroblastic appearance to a polygonal shape when transferred from the complete medium of DMEM-LG to a defined medium. A large amount of endoplasmic reticulum was observed in large Golgi ccmplex and mitochondria. The differentiation of MSCs toward chondrogenic phenotype was verified by the positive result of collagen type II through immunohistochemistry and in situ hybridization respectively.

CONCLUSIONSBone marrow stem cells obtained from adult swine can differentiate to be chondrogenic phenotype when cultured in vitro. MSCs can likely be served as optimal autogenous cell source for cartilage tissue engineering.

Animals ; Bone Marrow Cells ; physiology ; ultrastructure ; Cell Differentiation ; Cells, Cultured ; Chondrocytes ; physiology ; Collagen Type II ; genetics ; Phenotype ; RNA, Messenger ; analysis ; Stem Cells ; physiology ; ultrastructure ; Swine ; Tissue Engineering ; Transforming Growth Factor beta ; physiology