Objective To compare the prognosis efficacy between total laparoscopic gastrectomy and or-dinary gastric cancer for familial gastric cancer.Methods The selected 60 patients with ordinary gastric cancer from August 2012 to 2014 in our hospital as the control group,the same period in familial gastric cancer of 40 pa-tients were chosen as control group.All patients were performed by total laparoscopic gastrectomy.Results The operative time,blood loss,postoperative discharge time compared in the two groups were showed no significant differences.However,the postoperative hospital stayed in the observation group and the number of lymph nodes were significantly longer more than the control group( P<0.001) .The postoperative pulmonary infection,intesti-nal obstruction,abdominal infection,anastomotic leakage,anastomotic bleeding and other complications in the ob-servation group were significantly lower than in the control group( P<0.001) .After the investigation,the dyspha-gia,pain,anxiety,physical appearance,dry mouth scores in the observation group were significantly higher(P<0. 001).Conclusion Familial gastric cancer has unique clinical and pathological features,Compared to ordinary stomach,the total laparoscopic gastrectomy treatment of familial gastric cancer need more lymph nodes dissection, it can cause more complications,it has some impacts on the quality of life for patients.

Objective To discuss the influence of age factor on the outcome of the treatment of solitary bone cyst by Depo-Medrol infiltration. Methods 24 cases with Solitary Bone Cyst were analyzed. Age ranged from 4 to 23 years old,mean 10.8. Female 9 cases,male 15 cases. Humerus 18 cases,femur 2,tibia and fibula 2,radius and ulna 1, pelvis 1.16 cases(66.6%)Solitary Bone Cyst at proximal,7 cases(29.2%)at distal,1 (4.2%)at medio. 16 cases (66.6%)on the right,8 cases(33.4% )on the left. All cases were treated with Depo-Medrol infiltration. Mean follow up period was 4.5 years; review every case's result by the X-ray and clinical signs and symptoms. Results Group A 8 cases (33.4%) healed, Group B 9 cases (37.5%) healed. There was no significant difference in the healing rate be-tween Group A and B (P=0.653). Conclusions We conclude that the healing rate can not affected by age (P=0.653). In our opinion,the origin of Solitary Bone Cyst is disturbance of bone growth on physis,so we suggest the in-sertion point of inject needles close to the physis and sufficient to touch the physis.

Objective To discuss the opportunity of operation and selective in the early stage of treatment method. Methods To analyze the clinical data of 31 cases of patient traumatic floating knee since June 1999 to Au-gust 2006 at my hospital. The results of operative treatment and clinical condition were reviewed. Results In this group there were wound infection 5 cases,amputation 1 case,two stage plant the bone technique and inner fiction 2 cases. After following up patients 1 to 3 years(average 25 months). All of the patients were recovered. According to kerstin at working out standard the limbs function of floating knee damage was evaluated so as to obtain the good percentage of 87 % of the knee joint function. Conclusion A.s usually, the complication of multiple organs injury in floating knee damage was most likely to occur if there had been position priority disposed of important internal organs damage and shock strives in 24 hours properly. The fixed thighbone and shinbone fracture with the emergency dis-posal of important blood vessel damage can reduce the amputation rate.

Objective To evaluate the prognostic significance of metastastic lymph node ratio in patients with gastric cancer. Methods Clinical data of 319 patients with gastric cancer who underwent radical gastrectomy with at least 15 lymph nodes dissected per patient were analyzed retrospectively. Patients were divided into 4 groups according to the TNM staging system (1997,5th edition): pN0 (no positive lymph node), pN1(with 1～6 metastatic lymph nodes), pN2 group (with 7～15 metastatic lymph nodes), and pN3 group (with more than 15 metastatic lymph nodes), or into 4 groups according to the lymph node metastatic ratio: rN0 group (without metastasis of lymph node), rN1 group (with a lymph node metastatic ratio of <30%), rN2 group (with a lymph node metastatic ratio of 30%～60%), and rN3 group ( with a lymph node metastatic ratio of >60%). Survival was determined by the Kaplan-Meier method and differences were assessed by the Log-rank test. Multivariate analysis was performed using the COX proportional hazard regression model in forward stepwise regression with SPSS 13.0. Results Kaplan-Meier survival analysis demonstrated that the metastatic lymph node ratio significantly influenced the postoperative survival time. According to the lymph node metastasis system 77 patients were in group 1, 109in group 2, 60 in group 3, and 73 in group 4. The 2-year survival rate was 84.4%,66.1%, 35.0%,and 15.1% respectively, 3-year survival rate was 80.5%,45.0%,15.0% and 5.5% respectively, and 4-year survival rate was 71.4%,33.0%,5.0% and 1.4% respectively. If cases with the same positive number of lymph node (pN) group were stratified futher by lymph node metastatic ratio, there were no significant differences in the 3-year survival rate among the new rN groups (P>0.05), but there was a great difference in the distribution of prognosis among different groups. COX proportional hazard regression model analysis showed that the metastatic lymph node ratio was an independent factor for poor prognosis. Lymph node metastatic ratio was closely related with the location, modality of the operation and infiltration depth. Conclusions The staging system based on metastatic lymph node ratio is more reliable than the system based on the number of metastatic lymph nodes in the prediction of the prognosis of gastric cancer.

Objective To explore the changes of MR diffusion imaging (DWI) appearance in newborn rats with hypoxic‐ischemic brain damage(HIBD) ,and its relationship with the changes of Triphenyl tetrazolium chloride (TTC) staining .Methods Using liga‐tion of the left carotid artery method to establish three different degrees of HIBD animal models ;DWI was performed at each time point(6-12 h ,12-24 h ,3 d ,7 d);Fresh brain tissue taken from another model groups of newborn rats in 12 h ,24 h ,3 d ,7 d were staining in TTC ,then we observed its relationship with DWI .Results The lesion location of three model groups mainly distributed in the left side of cortex and subcortical region ,with prolonged hypoxia time ,hippocampus ,lateral side white matter ,thalamus were also have varying degrees of involvement .The right side of the cortex and subcortical in some cases involved .TTC staining showed posi‐tive results in 3 d ,its loss stained area were consistent with DWI abnormal signal area .Conclusion DWI can be evaluation of HIBD lesions early .The early lesions of HIBD mainly distributed in the left side of cortex and subcortical region .

Objective To analyze the growth and development condition of the children with cerebral palsy,and to investigate the influence of puberty on their adult height.Methods 56 children with cerebral palsy were selected as research subjects.34 healthy children were selected as control group.Their height and weight were measured,the development condition of their sexual character was checked,and then standard deviation of height and weight,height age and bone age were calculated,adult height and target height were predicted and analyzed.Results The standard deviation of height and weight in cerebral palsy group was (-1.29 ± 1.39) and (-0.77 ±1.20) respectively,which was lower than that of the normal control group[(0.40 ±0.95),(0.38 ± 1.01)] (t =-6.270,-4.6 7 6,all P ＜ 0.0 5).In preadolescent cerebral palsy group,the chronobiological age was bigger than their height age and bone age,the difference was significant (t =6.381,7.939,all P ＜ 0.05),but there was no significant difference between height age and bone age (P ＞ 0.05),there existed no significant difference between predicted adult height and target height(P ＞ 0.05).In adolescem cerebral palsy group,the chronobiological age and bone age were larger than their height age,which indicated significant difference (t =3.438,-3.759,all P ＜ 0.05),but there was no significant difference between the chronobiological age and bone age (P ＞ 0.05),the predicted adult height was lower than target height,the difference was significant (t =-5.204,P ＜ 0.05).Conclusion The growth and development of children with cerebral palsy would usually fall behind the normal children,but showed similarity in terms of starting age and process of puberty.After puberty,their bone age would increase dramatically,their predicted adult height would fall behind target height distinctly.

Objective By extra-body imitation release test with prolongation,medicines action on sustainedreleased injection of hydro-cortisone microeapsule was understood. Methods Making use of HPLC method was inspected to result of medicinal lower and die, solution. At the same time, the disaolvent course of microcapsule wall was observed by microscope and electron microscope. Results The release test at beginning 12 - 24 hours with rdease drugs measure was very lower-degree,along with the time more prolong in mierocapsule substance to contact of PBS solution(24～48h) was began disintegrate. The rdease of medicinal concentration also accompany with rising, the rising different concentration PBS solution from starting up to the highest as to general need 3～5 days and keep releasing for several days. Conclusion Sustained-released injection of hydrocortisone mierocapsule can be effectual action of prolongation released time in hind hydrocortisone.

Objective To assess the value of primary resection and anastomosis with intraoperative colonic defecation in the patients with obstructive left colonic cancer. Methods From January 2000 to January 2008, 39 patients undergoing emergency laparotomy for left colonic cancers with complete obstruction were analyzed retrospectively. Results The patients were 25 males and 14 females, with a median age of 68.5 years (range: 57～78 years). The primary tumors were located at splenic flexure (3/7.7%), descending colon (8/20.5%), sigmoid colon (15/38.5%), boundary of sigmoid colon and rectum (8/20.5%), and superior segment of rectum (5/12.8%). Primary resection and anastomosis with intraoperative colonic de-fection were performed in 18 patients with left hemicolectomy, 13 patients with sigmoid colectomy and 8 pa-tients with anterior resection. Early complications included wound infection in 4 patients (wound disruption in 1 patient) and pulmonary infection in 5 patients. One patient complicated with anastomotic leakage and intra-abdominal abscess died of tumor metastasis after reoperation. Another one died of respiratory failure secondary to pulmonary infection. Morbidity and mortality was 25.6% and 5.1% respectively. Conclusion Primary resection with intraoperative colonic defecation can be applied to patients with malignant colonic complete obstruction with good operative results.

Objective To assess the value of primary resection and anastomosis without intraoperative irrigation in the patients with obstructive left colonic cancer. Method Between January 2000 and January 2008, 93patients underwent primary resection and anastomosis for colonic cancers were analyzed retrospectively. Primary resection and anastomosis without intraoperative colonic irrigation (decompression by manual defecation) was performed in 43 patients with obstructive left colonic caner and traditional left-sided colectomy in 50 cases without obstruction. Both groups of patients were comparable in terms of gender, nutritional status, underlaying disease, tumor location and stage, etc ( P = 0.83,0.13,0.29,0.51,0.38). The average age of the patients with colonic obstruction was significant older than that of the cases without obstruction (61.2 ± 8.6 vs. 58.1 ±7.8, P =0.010).The operative results were compared between patients with obstructive colonic cancer and cases without obstruction.Results The mean hospital stay of the primary anastomosis group and traditional left-sided colectomy group were (16.6±7.8) d and (12.4±5.4) d respectively, and the former was significant longer than the latter (P =0.002). The costs of hospitalization in the two groups were (50192.8 ± 39727.4) RMB and (46489.3 ±29543.1)RMB respectively (P = 0.04) . The morbidity and mortality in the two groups were 25.6% (11/43) vs. 18%(9/50) (P =0.375) and 2.3% (1/43) vs. 2.0% (1/50) (P =0.714) respectively, and there were no significant difference between the two groups. Conclusions Primary resection and anastomosis without intraoperative colonic irrigation (decompression by manual defecation) compares favorably with traditional left-sided colectomy in safety and efficiency for left colonic cancer with obstruction.

Objective To investigate the effects of aging on procollagen α polypeptide gene transcription and protein expression in rat vascular smooth muscle cells.Methods Vascular smooth muscle cells from thoracoabdominal aorta in neonate and 9 months old healthy Wistar rats were cultured in vitro.Results Transcription polymerase chain reaction(RT PCR) and Western blotting were used to detect type Ⅰ and Ⅲ pro-collagen α polypeptide mRNA and protein.The RT-PCR displayed that type Ⅰ procollagen α polypeptide mRNA expression had no significant difference between young group and adult group [(76.62±1.05) vs.(78.37±2.42),P＞0.05].Type Ⅲ procollagen α polypeptide mRNA expression was (105.40 ± 2.66) in young group and (123.10 ± 3.81) in adult group(P＞0.05).Type Ⅰ procollagen α polypeptide mRNA expression was (3.13 ±0.54) in young group and (4.63 ± 1.03) in adult group (P=0.05).Type Ⅲ procollagen α polypeptide mRNA expression had no significant difference between the adult and young groups[(6.86 ±0.41) vs.(7.68±0.63),P＞0.05].Type Ⅰ and type Ⅲ procollagen α polypeptide protein expressions were increased significantly in adult group as compared with the young group [(0.10 ± 0.03) vs.(0.06±0.03),(0.58±0.06) vs.(0.40±0.02),both P＜0.05].Conclusions Aging increases the procollagen α polypeptide level in vascular smooth muscle cell,which may involve in the development of vascular remodeling and atherosclerosis.