Objective To explore the changes of MR diffusion imaging (DWI) appearance in newborn rats with hypoxic‐ischemic brain damage(HIBD) ,and its relationship with the changes of Triphenyl tetrazolium chloride (TTC) staining .Methods Using liga‐tion of the left carotid artery method to establish three different degrees of HIBD animal models ;DWI was performed at each time point(6-12 h ,12-24 h ,3 d ,7 d);Fresh brain tissue taken from another model groups of newborn rats in 12 h ,24 h ,3 d ,7 d were staining in TTC ,then we observed its relationship with DWI .Results The lesion location of three model groups mainly distributed in the left side of cortex and subcortical region ,with prolonged hypoxia time ,hippocampus ,lateral side white matter ,thalamus were also have varying degrees of involvement .The right side of the cortex and subcortical in some cases involved .TTC staining showed posi‐tive results in 3 d ,its loss stained area were consistent with DWI abnormal signal area .Conclusion DWI can be evaluation of HIBD lesions early .The early lesions of HIBD mainly distributed in the left side of cortex and subcortical region .

Objective To investigate the effects of aging on procollagen α polypeptide gene transcription and protein expression in rat vascular smooth muscle cells.Methods Vascular smooth muscle cells from thoracoabdominal aorta in neonate and 9 months old healthy Wistar rats were cultured in vitro.Results Transcription polymerase chain reaction(RT PCR) and Western blotting were used to detect type Ⅰ and Ⅲ pro-collagen α polypeptide mRNA and protein.The RT-PCR displayed that type Ⅰ procollagen α polypeptide mRNA expression had no significant difference between young group and adult group [(76.62±1.05) vs.(78.37±2.42),P＞0.05].Type Ⅲ procollagen α polypeptide mRNA expression was (105.40 ± 2.66) in young group and (123.10 ± 3.81) in adult group(P＞0.05).Type Ⅰ procollagen α polypeptide mRNA expression was (3.13 ±0.54) in young group and (4.63 ± 1.03) in adult group (P=0.05).Type Ⅲ procollagen α polypeptide mRNA expression had no significant difference between the adult and young groups[(6.86 ±0.41) vs.(7.68±0.63),P＞0.05].Type Ⅰ and type Ⅲ procollagen α polypeptide protein expressions were increased significantly in adult group as compared with the young group [(0.10 ± 0.03) vs.(0.06±0.03),(0.58±0.06) vs.(0.40±0.02),both P＜0.05].Conclusions Aging increases the procollagen α polypeptide level in vascular smooth muscle cell,which may involve in the development of vascular remodeling and atherosclerosis.

The ompA gene of Chlamyia psittaci in cows was amplified by PCR with primers designed based on those reported in GenBank.The amplified ompA gene was inserted into the bacterial plasmid vector pGEX-4T-1 and then transformed into E.coli BL21(DE3) with IPTG induction. The gene was derived from plasmid pMD18-T vector and then sequenced.It was demonstrated that this recombinant fusion protein of approximately 68kD in molecular mass was highly expressed in inclusion body and more pure proteins would be produced after purification.The fusion protein specifically reacted with positive sera of bovine Chlamydia as demonstrated by Western blotting. These results indicate that this recombinant fusion protein shows good reactivity and could be used to develop the diagnostic kit for bovine Chlamydia and genetic engineering vaccine.

Objective:To explore the application effect of case reporting model in newly recruited nurses.Methods:A total of 149 newly recruited nurses in First Hospital of Qinhuangdao from August 2016 to August 2018 were selected as the observation group. A total of 79 newly recruited nurses from August 2014 to August 2015 were selected as the control group. The newly recruited nurses in the observation group were trained in accordance with the requirements of the routine Syllabus for Newly Recruited Nurses and reported cases for 6 months, once a month. The control group was trained in accordance with the requirements of the Syllabus for Newly Recruited Nurses. The head nurses evaluated and compared the professional theoretical ability, active communication ability, problem solving ability, work cooperation ability, emergency capability, expression and communication ability and PPT production ability of the two groups of newly recruited nurses.Results:In the evaluation of newly recruited nurses by head nurses, the excellent rates and the good rates of professional theoretical ability, active communication ability, problem solving ability, work coordination ability, emergency capability, PPT production ability, expression and communication ability of newly recruited nurses in the observation group were all better than those in the control group, and the differences were statistically significant ( P<0.05) . Conclusions:The implementation of the case report model in standardized training of newly recruited nurses can promote active learning of newly recruited nurses, enhance the ability of newly recruited nurses to analyze the disease, and improve their job competence.

Objective To develop an evaluation management information system for new nurses(EMISNN),and to explore the effectiveness of its application in the training of new nurses.Methods A research and development team was set up to design and build the EMISNN.EMISNN is a system which comprises 3 main modules,namely data integration module,query statistics module and strategy generation module.From August 2021 to April 2023,the new nurses of Qinhuangdao city,a tertiary A hospital who joined in 2022 were selected as an experimental group,and the new nurses joined in 2021 were selected as a control group by the convenience sampling method.The experimental group adopted the data analysis and strategies provided by the EMISNN to guide the training of the theory and practice of surgery,while the control group adopted the routine training plan,and then the performance of the 2 groups nurses in the theory test of surgical nursing and their satisfaction with the training were compared.Results After training,the experimental group was(73.51±8.51)points,while the control group was(69.14±8.01)points,and the difference between the 2 groups was statistically significant(t=3.112,P=0.002).The training satisfaction of the experimental group was(34.53±2.80)points,while the control group was(32.42±2.70)points,and the difference between the 2 groups was statistically significant(t=4.486,P＜0.001).Conclusion The application of the EMISNN conduces to the precision and intelligence of new nurses trainings,and it is also conducive to enhancing the satisfaction of new nurses with the training and improves the training quality.