Object To study the chemical constituents of alkaloids of Amsonia sinensis Tsiang extracted with methanol. Methods Compounds were separated by exchange resin, macroporous resin and silica gel column chromatography. Their structures were elucidated by spectroscopic analysis. Results Three compounds were isolated from total alkaloids of A. sinensis extracted with methanol. They were identified as: rhazidigenine (Ⅰ), amsonic acid (Ⅱ), trans-sinapic acid methylester (Ⅲ). Conclusion Compound Ⅱ is a new one, and compound Ⅰ and Ⅲ are obtained from this plant for the first time.

Object A systematic study on the chemical constituents of Incarvillea arguta (Royle) Royle was carried out Methods The constituents were isolated on silica gel column chromatography, preparative TLC and Sephadex LH 20 column chromatography, identified by physicochemical properties and the sturctures were elucidated by spectral analysis (MS, 1HNMR, 13 CNMR) Results Three compounds were identified as argutine A (Ⅰ), argutine B (Ⅱ) and boschniakinic acid (Ⅲ) Conclusion Compound Ⅰ and Ⅱ are new monoterpene alkaloids, and compound Ⅲ is isolated from Incarrillea A Juss plants for the first time

Objective To measure normal range of CD4 from Chinese adults in Shanghai area by flow cytometry. Methods CD4 and CD8 value were measured through flow cytometry in adult blood specimens from various age and gender group. Results There were no difference in CD4 value among different sex and age groups, with the mean value being 726.99?255.21. However, CD8 and CD4/CD8 ratio vary significantly among different groups. The mean value of CD8 was 539.58?134.07, while the mean value of CD4/CD8 1.49?0.57. Conclusions The mean value of CD4 in Shanghai adults is about 100/mm 3 lower than that in American.

Objective To construct recombinant plasmid with p24 and gp41 gene, express fusion protein in E.coli. Methods To design primer with restriction endonuclease position,and amplify p24 and gp41 by RT-PCR, link both into pMD18-T vector.To choose correct clone with target gene.Then p24 fragment will be cleaved and linked into pMD18-T vector within gp41 gene. Both post-linked gene will be cleaved and linked into pET21a vector. The vector will be transformed into E.coli. And protein is highly effective expressed in E coli. Western blotting proved that the expressed product could react with 6?his antibody. Result Fusion protein p24-gp41 is highly effective expressed in E.coli. Conclusion Fusion protein p24-gp41 is highly effective expressed in E.coli in pET21a vector.

Objective: To study the executive and attention function of patients with depression. Methods: We used the Wisconsin Card Sorting Test (WCST) and Continue Performance Test (CPT) to study the executive and attention function in 66 patients with schizophrenia, 42 patients suffering from depression, and 50 normal controls. Results: Schizophrenic patients and depressive patients performed worse than the normal controls in WCST (P

From the total alkaloids of the root of Kopsia lainanensis Tsiang, eight compounds were isolatcd and identificd as coronaridine (Ⅰ), heyneaninc (Ⅱ), tabersonine (Ⅲ), 11-methoxytabersonine (Ⅳ), scandine (Ⅴ), N-methoxycarbonyl-11, 12-methylenedioxykopsinaline (Ⅵ), kopsinine (Ⅶ) and N-methoxycarbonyl-12-methoxykopsinaline (Ⅷ) by means of physical constants and spectral analysis

Objective To explore the application value of spiral CT angiography in etiological diagnosis of spontaneous intracranial hematoma before surgery. Methods 48 patients with spontaneous intracranial hematoma following surgical indication assessed by CT scan were ascertained etiological diagnosis performed by CT angiography before surgery.Results The causes of intracranial hematoma were cerebral aneurysm(2 patients), arteriocenous malformation (5 patients), hypertension(40 patients), and no image in one patient performed by CT angiography.Conclusion CT angiography has important value in etiological diagnosis of spontaneous intracranial hematoma before surgery.

Objectives To analyze the characteristic of HIV-1 viral infectivity factor (Vif) gene variants isolated from Shanghai. To construct the prokaryotic expression vector of HIV-1 vif gene and understand its immunogenieity. Methods HIV-1 vii genes were amplified and sequenced from 23 serum samples of HIV-1 infected patients in Shanghai and then compared with the international standard HIV-1 strain. Subsequently, these amplified Vif fragments were sub cloned into pETS2b(+)expression vector. The recombinant prokaryotie plasmids pETS2b (+)-HIV-1/Vif were then transferred into BL21DS(Star)cells for expressing and purifying HIV-1 Vif protein. HIV-1 Viff rat polyclonal antibody was then preparing by injecting the purified Vif proteins into the mice. ELISA was used to determine the purity of Vif proteins and the immunogenicity of its polyclonal antibodies. Results The nucleotide acid mutation rate of HIV-1 vif gene in Shanghai AIDS patient was (0. 179±0. 006)% compared with the international standard HIV-1 strain. Some similar mutations in vif gene were found in HIV-1 strains isolated from Shanghai while the amino acid sequence between 151 and 240 in Vif protein was conserved. The construction of HIV-1 Vif prokaryotic expression plamids and the preparation of Vif polyclonal antibodies were successfully done in this study. The reaction between recombinant HIV-1 Vif protein and the serum from HIV-1 infected patient was not significantly different from that between the recombinant protein and healthy control serum(P＞0.05). HIV-1 Vif polyclonal antibody reacted differently with recombined HIV-1 Vif protein compared with healthy control serum samples (t=178.61, P＜0.01). Conclusions The Vif gene mutation rate is high in HIV-1 strains isolated from Shanghai compared with international standard HIV-1 strain. The prokaryotic expression plasmids of HIV-1 vif antigen are successfully constructed and Vif polyclonal antibodies are prepared well.

Objective To investigate the clinical application effect of independently designed subcutaneous tunnel in endo‐scopic thyroid operation .Methods A total of 480 cases of endoscopic thyroid operation were completed in our department from February 2005 to December 2014 ,in which 315 cases adopted the independently designed subcutaneous tunnel for separating the subcutaneous tissues in anterior thoracic region and the other 165 cases used the puncture stick for separating and constructing the subcutaneous tunnel .Results Using independently designed subcutaneous tunnel for constructing the subcutaneous tunnel in the anterior thoracic region had statistical difference in the operative time and intraoperative bleeding amount compared with the punc‐ture stick(P<0 .05) ,and the difference of postoperative complications between the two groups was statistically signifficant (P<0 .05) .Conclusion Adopting the independently designed subcutaneous tunnel for constructing subcutaneous tunnel has the advan‐tages of short time consuming ,less bleeding and fewer complications .The level and size of the constructed subcutaneous tunnel are easy to grasp ,so which is more convenient for endoscopic thyroid operation .

Aging changes of somatostatin (SS) mRNA-positive neurons in the dorsal hippocampus of old rats were studied by in situ hybridization histochemistry and image analysis. In the young rats,SS mRNA-positive cell bodies were mainly located in the pyramidal and polymorphic cell layers of the CA1 and CA2 regions, and in the strata radiatum and polymorphic cell layer of the CA3 region, and in the polymorphic cell layer of dentate gyrus. In the old rats,SS mRNA-positive cell bodies were concentrated in the polymorphic cell layer and denate gyrus. A marked decrease of number of SS mRNA-positive cell bodies and a significant increase of gray value of SS mRNA-positive cell bodies were found in the dorsal hippocampus and dentate gyrus of the old rats as compared with the young rats. No significant age difference in the sectional area of SS mRNA-positive cell bodies was observed in the dorsal hippocampus. The results showed that there were apparent changes with aging of the SS mRNA-positive cell bodies in the dorsal hippocampus of the rats. The significance of the changes remains to be studied further.