1.Microfluidic chip digital PCR technology and its clinical application
Chinese Journal of Laboratory Medicine 2015;(9):592-594
Microfluidics-based digital PCR depends on microfluidic chip to split PCR reaction mixture into many tiny equal-volume units.Quantitative assessment of target DNA template can be obtained by counting the number of fluorescence-positive units after thermocycling.Microfluidics-based digital PCR exhibits many advantages including absolute quantification, high sensitivity and accuracy, and shows great promise in a variety of applications, such as infectious diseases diagnose, early cancer detection and prenatal diagnose. There are already several microfludics-based digital PCR products produced from sereval companies.It is believed that as the technology improves, microfluidics-based digital PCR will find broader applications and become the next-generation tool for genetic tests.
2.Optimization of Extraction Technology of Polysaccharides from Cipangopaludina chinesis by Enzyme Method
Xiaoyan LIU ; Chaopin LI ; Tao ZHU
China Pharmacy 2015;(19):2708-2710
OBJECTIVE:To optimize the extraction technology of polysaccharides from Cipangopaludina chinesis by enzyme method. METHODS:The papain enzymolysis extraction was performed. On the basis of single factors tests,taking the yield of polysaccharides as the index,four factors including enzyme concentration,pH,enzymolysis temperature and enzymolysis time in the extraction process were optimized by the orthogonal test,and verification was carried out. RESULTS:The optimal technology by enzyme method was as follows as enzyme concentration of 1.0%,pH of 7.0,enzymolysis temperature of 55 ℃ and enzymoly-sis time of 3.0 h. Under this technology,the average yield of polysaccharides was 4.98%(RSD=2.51%,n=3)and the content of polysaccharides was 73.54%(RSD=1.36%,n=3). CONCLUSIONS:The optimal extraction technology of polysaccharides from C. chinesis was simple,reasonable and feasible.
3.Composition and Diversity of Acaroid Mite Community in Houses in Xuancheng Area
Xiaoyan LIU ; Chaopin LI ; Li TAO
Journal of Environment and Health 1993;0(03):-
Objective To investigate the composition and diversity of acaroid mite community in the house in Xuancheng area in Anhui province.Methods The dust samples from 4 sites selected in each house were collected in June-July,2006,acaroid mites were isolated,counted and identified.Results 3 386 acaroid mites were obtained,belong to 6 families,12 genera and 14 species.The species richness index Rmargalef for the four habitats ranged from 0.73 to 1.75,while the species diversity index(Shannon-Wiener index) ranged from 1.36 to 1.87 and Pielou index ranged from 0.70 to 0.92.Conclusion The results of the present paper show that the habitat condition is the main factor that can directly influence the community structure and diversity of acaroid mites in the houses and the human activity is also an influencing factor to be reckoned with.
4.Intravitreal injection of conbercept for wet age-related macular degeneration
Lei ZHU ; Xiaoyan TIAN ; Tao LIU
Recent Advances in Ophthalmology 2017;37(5):473-476
Objective To observe the efficacy and safety of intravitreal injection of conbercept for wet age-related macular degeneration.Methods Retrospective clinical study.Sixty eyes of 60 cases diagnosed as wet age-related macular degeneration (AMD) definitely were intravitreally injected conbercept 0.5 mg from March 2014 to March 2016.Follow-up time was 6 months to 12 months,averaged (8.5 ± 2.4) months.Visual acuity (ETDRS charts letter),central macular thickness (CMT),leakage of choroidal neovasculaxization (CNV) and area of CNV and complications before and after the treatment were analyzed.Results Conbercept injection therapeutic times were 1-5,the average therapeutic times were 2.80 ± 0.89.At 1 month,2 months,3 months and 6 months after treatment and the last follow up,the mean letter of ETDRS charts increased (14.76 ± 5.89) letters,(19.88 ± 7.13) letters,(24.75 ± 6.74) letters,(23.94 ±6.15) letters,(22.89 ± 8.53) letters compared with pre-operation,respectively (all P <0.05).CMT decreased (70.19 ± 60.56) μm,(82.07 ± 57.97) μm,(95.40 ± 87.92) μm,(97.57 ± 46.68) μm,(107.46 ± 56.82) μm compared with pre-operation,respectively (all P < 0.05).Before treatment,the area of CNV leakage was (12.32 ± 5.67) mm2,and at postoperative 3 months,6months the area were statistically significant different (all P < 0.05).Four cases had subconjunctival flake bleeding,intraocular pressure increased slightly in 3 cases and they all recovered at 1 week after treatment.There was no serious ocular and system complication.Conclusion Intravitreal injection of conbercept for wet AMD can improve the visual acuity,decrease CMT and inhibit the CNV regression without serious complication.
5.Nursing care of a patient with hemophilic arthritis and hepatitis C undergoing unilateral hip and knee replacement simultaneously
Xiaoyan DONG ; Guiyan TAO ; Juan ZHAO ; Li ZHANG ; Xiaojuan LI
Chinese Journal of Practical Nursing 2021;37(9):688-694
Objective:To summarize the perioperative nursing experience of a patient with hemophilia arthritis complicated with hepatitis C undergoing unilateral hip and knee arthroplasty at the same time.Methods:In August 2018, a patient with hemophilic arthritis complicated with hepatitis C was admitted to the first hospital of Lanzhou University. Preparations for health education, functional exercises, examination and evaluation before operation was made. Unilateral hip and knee arthroplasty were performed at the same time, strengthen the management of incisions and catheters after the operation, actively prevent complications, and guide patients to perform functional rehabilitation exercises under the conditions of good pain management and safe coagulation factor activity; pay attention to the nursing of coagulation factor replacement treatment in the whole perioperative period.Results:After careful nursing, no serious complications occurred during the patient's hospitalization, and the position and force line of the prosthesis were satisfactory by imaging examination before discharge.Conclusion:The perioperative care of this patient proved that the above-mentioned nursing measures are effective for hemophilic arthritis patients with hepatitis C undergoing unilateral hip and knee arthroplasty at the same time.
6.Study on quality standard for Xiaozhi Tablets
Xiaoyan DING ; Songqing YAN ; Weke JIANG ; Tao ZHOU ;
Chinese Traditional Patent Medicine 1992;0(05):-
AIM: To establish the quality standard for Xaozhi Tablets(Lasiosphaera seu Calvatia, Rhizoma Dioscoreae Cirrhosae, Radix Sophorae Tonkinensis, Concha Ostreae, etc.). METHODS: Rhizoma Dioscoreae Cirrhosae and Radix Sophorae Tonkinensis were identified by TLC. Matrine was determined by HPLC. RESULTS: Rhizoma Dioscoreae Cirrhosae and Radix Sophorae Tonkinensis could be detected by TLC. Matrine showed a good linear relationship in the range of 0.2040~2.040?g and the average recovery was 97.64%, RSD was 1.49%. CONCLUSION: The methods are simple, sensitive and accurate with good reproducibility. The methods may be used for the quality control of Xiaozhi Tablets.
7.Protective mechanism of rosiglitazone on acute necrotizing pancreatitis associated lung injury in rats
Tao YIN ; Xiaoyan CHEN ; Youming DING ; Chen CHEN ; Weixing WANG
Chinese Journal of Pancreatology 2013;13(4):259-262
Objective To investigate the protective effect of rosiglitazone on acute necrotizing pancreatitis (ANP) associated lung injury in rats.Methods Seventy-five male Wistar rats were randomly divided into sham operation group (SO group),acute necrotizing pancreatitis group (ANP group) and rosiglitazone pretreatment group (ROSI group).ANP model was induced by retrograde infusion of 5% sodium taurocholate into the biliopancreatic duct.Thirty minutes after ANP induction,ANP groups were injected with 10% DMSO (0.2 ml/100 g) through femoral vein,and ROSI group were injected with ROSI dissolved with 10% DMSO (6 mg/kg) through femoral vein,while SO group was injected with normal saline,and 30 minutes later was injected with same amount of 10% DMSO.Rats were sacrificed at 3 h,6 h and 12 h after the operation.Serum amylase and lung wet/dry weight ratio (W/D) were measured,lung tissues were harvested for pathologic examinations.STAT1 protein and phosphorylation-STAT1 protein (p-STAT1) expression were detected by Western blot.Results The serum levels of amylase,lung W/D,pathologic score of lung tissues in ANP group were increased with time,and reached the peak at 12 h,which were (5017 ± 203)U/L,3.12 ±1.30,(3.33 ±0.18) score,and these were significantly higher than those in SO group (P < 0.05 or 0.01),the expression of STAT1 protein was not statistically significant,but the expression of p-STAT1 reached the peak at 3 h (5.23 ± 0.03),then it gradually decreased,but it was still significantly higher than that in SO group (0.16 ± 0.04,p < 0.01).The serum levels of amylase,lung W/D,pathologic score of lung tissues in ROSI group at 12 h were (1912 ± 164) U/L,1.83 ± 1.26,(2.78 ± 0.16),which were significantly lower than those in ANP group (P < 0.05).The expression of STAT1 protein was not statistically significant,and the expressions of p-STAT1 at 3 h,6 h,12 h was 0.41 ±0.04,0.22±0.05,0.15 ±0.03,which were significantly lower than those in ANP group (P < 0.05).Conclusions Rosiglitazone has the protective effect on ANP associated lung injury by inhibition of phosphorylation-STAT1 protein expression in the early phase.
8.Clinical study of carboprost tromethamine combined with motherwort injection in prevention of high-risk ce-sarean section hemorrhage
Xiaoyan DING ; Tao FENG ; Hui GENG ; Fang LIU ; Airong MA
Chinese Journal of Primary Medicine and Pharmacy 2014;(7):1006-1007
Objective To investigate the carboprost ammonia butyl alcohol three plus motherwort injection on prevention of high risk maternal postpartum hemorrhage effect .Methods 120 cases of cesarean section operation and potential bleeding symptom of women were selected as the research object ,and randomly divided into two groups , the observation group of 60 cases using carboprost ammonia butyl alcohol three joint of leonurus heterophyllus injec -tion for prevention and treatment ,the control group of 60 cases used oxytocin for prevention and treatment ,prevention and treatment effects were compared between the two groups .Results After treatment,the observation group 2h,24h postpartum hemorrhage were (422.41 ±213.49)mL,(35.29 ±16.44)mL,were significantly less than those in the control group (589.64 ±345.21)mL,(69.31 ±29.47)mL(t=6.732,8.915,all P<0.05);the observation group 1 cases without production after bleeding ,control group 5 cases of postpartum hemorrhage ,statistically significant differ-ences between the two groups (χ2 =4.973,P<0.05).Conclusion Carboprost ammonia butyl alcohol three plus motherwort injection on prevention of high risk maternal postpartum hemorrhage has a significant effect ,which can re-duce the cesarean section uterine bleeding and reduce the amount of bleeding ,promote uterine involution ,conducive to maternal rehabilitation .
9.Determination of antioxidant capacity and 8-iso-prostaglandin F2α levels in patients with psoriasis and their significance
Xiaoyan JIAO ; Zaipei GUO ; Tao CHEN ; Yuhong ZHANG ; Mengmeng LI
Chinese Journal of Dermatology 2012;45(6):388-391
ObjectiveTo determine the levels of 8-iso-prostaglandin F2α (PGF2α) in sera and lesions as well as antioxidant capacity in sera of psoriatic patients,and to assess their correlations with disease severity.MethodsSerum and skin tissue samples were collected from 15 healthy controlsand 50 patients with psoriasis vulgaris.Spectrophotometry was performed to determine the levels of total antioxidant capacity (T-AOC) and the activities of antioxidant enzymes such as superoxide dismutase (SOD),catalase (CAT) and glutathione peroxidase (GSH-Px) in serum samples.Enzyme-linked immunosorbent assay (ELISA) and immunohistochemical SP method were carried out to detect the expression level of 8-iso-PGF2α in the serum and tissue specimens respectively.ResultsThe psoriatic patients showed a significant decrease in the serum level of TAOC((12.78 ± 7.75) U/ml vs. (23.17 ± 8.81) U/ml,P< 0.01) as well as the activities of SOD((28.91 ±9.35) U/ml vs.(51.36 ± 7.92) U/ml,P< 0.01) and GSH-Px ((180.64 ± 47.70) U vs.(244.20 ± 66.68) U,P < 0.01 ) compared with the healthy controls.The serum T-AOC level and SOD activity were lower in patients with severe psoriasis than those with mild or moderate psoriasis ((9.06 ± 5.30) U/ml vs. (15.27 ± 8.18) U/ml,(21.63 ± 5.28) U/ml vs. (33.76 ± 8.28) U/ml,both P< 0.01 ),while there was no significant difference in the activity of GSH-Px between patients with severe and mild or moderate psoriasis.The serum CAT activity was significantly higher in patients with mild or moderate psoriasis than in the healthy controls and patients with severe psoriasis ( (36.92 ± 11.31 ) U/ml vs.( 28.55 ± 8.51 ) U/ml and (24.15 ± 9.36 ) U/ml,P < 0.05 and 0.01 ).Increased serum and lesional 8-iso-PGF2α levels were observed in psoriatic patients compared with the healthy controls ( (88.77 ± 25.27) ng/L vs.(38.34 ± 8.94) ng/L,0.0186 ± 0.0082 vs.0.0027 ± 0.0014,both P < 0.01),as well as in patients with severe psoriasis compared with those with mild or moderate psoriasis(( 114.24 ±13.93) ng/L vs.(71.78 ± 14.35) ng/L,0.0279 ± 0.0027 vs.0.0125 ± 0.0030,both P< 0.01 ).The psoriasis area and severity index(PASI) score was negatively correlated with T-AOC level,SOD and CAT activities(r =-0.384,-0.573 and -0.444,all P < 0.01 ),positively correlated with serum and lesional 8-iso-PGF2α levels (r =0.710,0.783,both P < 0.01 ),and uncorrelated with GSH-Px activity.None of the parameters was correlated with the course of disease.ConclusionThe serum and lesional levels of 8-iso-PGF2α may be a more sensitive marker for oxidative damage and disease severity.
10.The relationship between professional identity and professional self-concept among nurses
Aihua SUN ; Jing LI ; Yixiang TAO ; Jinqiu ZHANG ; Xiaoyan XU
Chinese Journal of Practical Nursing 2016;32(4):241-245
Objective To describe the status of nurses′professional identity and the self-concept and to explore the relationship between nurses′professional identity and the self-concept. This will be the base for intervening nurses′professional identity. Methods A convenience sample of 250 nurses from one major hospital in Beijing was recruited. The Macleod Clark Professional Identity Scale and the Professional Self-Concept Instrument were introduced. A standard translation procedure was carried out. Results A total of 250 questionnaires were sent out, 241 questionnaires were withdrawed with an effective rate of 96.4%. The mean score of nurses′professional identity was 42.54±8.70, which was at moderate level. The mean score of nurses′professional self-concept was 86.28 ±22.86, also at moderate level, among which,knowledgescores highest (25.91±6.00), leadership scored 20.84±8.02, inter-personal relationship scored (20.28±4.18), caring scored the lowest (19.25±4.67). There was significant difference among the professional identity in the different departments (P<0.05) , while the same results were not seen in professional self-concept. Nurses′professional identity was positively correlated with professional self-concept′s four dimensions (P<0.05). Conclusions The nurse managers should pay attention to the cultivation of professional self-concept in the training of nurses in order to improve the professional identity of nurses and the whole nurses′development.