Objective To analyze the prescriptions containing Panlongqi Pieces for clinical application;To guide reasonable usage of medicine in clinic. Methods Adopted retrospective investigation method by selecting 1178 prescriptions of Chinese patent medicine containing Panlongqi Pieces from January to March in 2014. The unreasonable elements in these prescriptions were analyzed. Results Among the 1178 prescriptions, there were 585 unreasonable prescriptions, occupying 49.66%. Problems in the application of Panlongqi Pieces in clinic lie in differentiation of syndromes, combination of medication, incompatibility of medicine in prescription, and usage and dosage. Conclusion Doctors should attach importance to differentiation and reasonable usage in clinical application, which can achieve expected therapeutic effect.

Objective To analyze the usage of TCM decoction pieces in our hospital during 2011-2014;To provide references for clinical reasonable medicine using. Methods Statistic analysis was carried out from the aspects of annual amount of prescription, dosage, annual sales, and top 10 TCM decoction pieces among sales rank through management system of hospital pharmacy. Results Sales amount and dosage of TCM decoction pieces increased significantly. During the four years, six same TCM decoction pieces ranked among the dosage of top 10 TCM decoction pieces, namely Astragali Radix, Paeoniae Radix Alba, Angelicae Sinensis Radix, Salviae Miltiorrhizae Radix et Rhizoma, fried Atractylodis Macrocephalae Rhizoma, and Chuanxiong Rhizoma. The amount of prescription increased obviously. Conclusion TCM decoction pieces are commonly used in our hospital;dosage increased continuously;usage is generally reasonable.

Objective To establish relevant management measures and guarantee the safety of clinical medication by analyzing the quality of warehouse entry inspection for Chinese decoction pieces.Methods According to the records of warehouse entry inspection for Chinese decoction pieces during January 2014 to December 2014, reasons for unqualified Chinese decoction pieces were collected and analyzed.Results Our hospital checked and accepted 2376 batches of Chinese decoction pieces, among which 98 batched were unqualified (4.12%); the reasons for unqualified Chinese decoction pieces mainly included improper storage, adulteration, and nonstandard preparation, which accounted for 48.97%, 19.39% and 18.37% respectively.Conclusion There are some problems in the Chinese decoction pieces in our hospital. Dispensary of TCM should enhance the management of check and acceptance of Chinese decoction pieces, and improve quality for check and acceptance and quality of Chinese decoction pieces.

The role of neutrophil in the pathogenesis of many diseases attracts more attentions than before. Neutrophil elastase (NE), a powerful neutrol proteinase capable of causing major tissue destruction in many diseases, is mainly present in the azurophil granules of neutrophil. A solid-phase enzyme-linked immunosorbent-biotin assay for human NE has been developed. Only 20?l of plasma sample is needed. Non-smoking healthy adults have 33.7?6.7ng NE/ml plasma; patients with chronic obstructive pulmonary diseases, 48.0+ 10.9 ng NE/ml; patients with pyogenic dermatisis, 69.1?15.7 ng NE/ml; patients with systemic lupus erythematosus, 61.0?13.9 ng NE/ml; patients with aplastic anemia, only 12.3?5.3 ng NE/ml. All of these results have significant differences compared with the result of healthy adults. The results of assay showed that this quantitative assay with good specificity and accuracy may be a better criterion for investigating the effect of NE on pathogenesis of some diseases.

Objective: To explore the effect of sequential external counterpulsation on sudden deafness.Methods: All the patients were treated with sequential external counterpulsation instrument produced by Guangzhou South-China Medical Instrument Company.Results: After treatment,the hearing threshold of 60 cases was all improved,with an increase from 5 to 53 db,and with an average threshold shift of 29?8.7db.86.7% of cases acquired satisfied therapeutic effects except 8 cases whose hearing threshold increased by less than 15db.Conclusion: the results showed that,the treatment of sudden deafness with Sequential External Counterpulsation has good therapeutic effects.Earlier and longer treatment means better effect

Based on the issue of medical English teaching, according to the immersion theory,we improved teachers' oral English, gave students a good foreign language circumstance, set students as the teaching center.to let them join the teaching activity and graspe medical English. All teaching reform worked well.

Objective To observe the change of renal morphological structure and apoptosis of renal cells. Methods Healthy adult male Wistar rats were randomly divided into control group(n=5),2 weeks CCl_4 group(n=5) and 6 weeks CCl_4 group(n=5).The control group were injected oily solution with 0.12ml/100g rat weight,the CCl_4 group were subcutaneously injected 60% carbon tetrachloride oily solution with 0.3ml/100g rat weight.At the first day of 3 weeks and 7 weeks,2 weeks CCl_4 group and 6 weeks CCl_4 group rats were respectively killed.The morphological structure in renal central section was observed after HE staining.The expressions of Caspase-3 and Bax were detected by immunohistochemistry.The apoptosis of rat renal cells was detected by TUNEL staining. Results The structures of rat renal tubules and corpuscle was normal in the control group.We detected the histological change in proximal tubules in the 6 weeks CCl_4 group,tiny tomentums of epithelial cells fell off,cells was vacuolization.The structure of renal corpuscle was normal.The pathological changes of 2 weeks CCl_4 group were similar,but relative little and limited.There was no Caspase-3 and Bax positive cell in control group and 2 weeks CCl_4 group,but lots of Caspase-3 and Bax positive cells were in 6 weeks CCl_4 group,mainly fastened on epithelial cells of proximal tubules near the medulla,and brown granules were mostly located in the cytoplasm.There was no positive cell in control group by TUNEL staining.There were a lot of apoptosis epithelial cells of proximal tubules,renal glomerular also had a few of TUNEL positive cells in the 2 weeks CCl_4 group.Apoptosis cells mainly located in the epithelial cells of distal tubules in the 6 weeks CCl_4 group,a few of apoptosis epithelial cells were in proximal tubules.Conclusion In carbon tetrachloride induced renal injuried rats,apoptosis cells mainly happened in epithelial cells of proximal tubules in 2 weeks.The apoptosis of epithelial cells of distal tubules presented 6 weeks later.

Objective To survey the unconventional blood sugar level induced by quinolones and promote the rational drug use in clinical. Methods Collecting and analysis the unconventional blood sugar level induced by quinolones reported during the period of 1995 ～ 2008 using CHKD net. Results 107 cases of unconventional blood sugar level were reported by using 9 kinds of quinolones and among them 70 cases with hypoglycemia and 3 cases died of hypoglycemic coma. Another 37 cases were found with hyperglycemia. Conclusion Should reinforce the supervi-sion of rational use of quinolones and monitor the blood sugar level when the elderly and the diabetic of renal insuffi-ciency in clinical use.

Lactoferrin has antitumoral activity. Lactoferrin exerts anti-carcinogenic effects via its ability to modulate host immunity, suppress proliferation, induce apoptosis, block cell cycle, inhibit angiogenesis, regulate carcinogen-metabolizing enzymes and sequester iron, etc. Safety and no toxicity of lactoferrin provide a new selection for cancer treatment.

BACKGROUND: Several studies have demonstrated that degradable polycaprolactone film has no apparent influences on cellular immune function of rat critical organs including heart, liver, and kidney, and possesses the prerequisite of anti-adhesion formation.OBJECTIVE: To investigate the role of degradable polycaprolactone film in preventing rat peritoneal adhesion following surgery.DESIGN, TIME AND SETTING: The present randomized, controlled animal experiment was performed at the Department of Physiology, Jilin University.MATERIALS: A total of 120 male Wistar rats, weighing 180-220 g, were included in this study. Peritoneal adhesion was induced in all rats. Non-bio-degradable film was sourced from Wuhan Boster Company, China. Polycaprolactone film was purchased from U.C.C Company. England.METHODS: All 120 rats were randomly divided into 3 groups, with 40 rats per group: control, non-bio-degradable film, and polycaprolactone film. Peritoneal adhesion was induced in all rats. The non-bio-degradahle film and polycaprolactone film groups were subjected to peritoneal wound cover with non-bio-degradable film and polycaprolactone film, respectively. From day 1 following surgery, each rat daily received an intraperitoneal injection of 4 mL 2 g/L pseudomonas enzyme, for a total of 3 successive days.MAIN OUTCOME MEASURES: At 1, 3, 7, and 30 days following surgery, 10 rats were selected from each group for observation of peritoneal adhesion.RESULTS: Totally 120 rats were included in the result analysis. Peritoneal adhesion was successfully induced in rats from the control group. From day 7 following surgery, the peritoneal adhesion rate was significantly lower in the polycaprolactone film group than in the control group (P<0.01). In the non-bio-degradable film group, the peritoneal adhesion rate exhibited decreasing followed by increasing tendency; by day 30, the adhesion rate was significantly higher compared to the polycaprolactone film group (P<0.01).CONCLUSION: Polycaprolactone film is degraded by pseudomonas enzyme to avoid the new adhesion caused by foreign stimulation.