1.Risk factors and re-myringoplasty for perforation after tympanic membrane repairing.
Guan-sen NI ; Yi QIAO ; Xiao ZHONG ; Wen-wen CHEN
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2005;40(8):625-626
Adolescent
;
Adult
;
Child
;
Female
;
Humans
;
Male
;
Middle Aged
;
Myringoplasty
;
Risk Factors
;
Tympanic Membrane Perforation
;
etiology
;
surgery
;
Young Adult
2.lnfluence factors of dry eye among aerospace science and technology staff and the health guidance
Qian-Wen, LI ; Xiao-Lin, HAO ; Zhong-Chen, ZHANG
International Eye Science 2014;(12):2240-2243
AlM: To investigate and evaluate the prevalence of xerophthalmia among aerospace science and technology staff and its influencing factors for the eye health guidance.
METHODS: The staff underwent physical examination in our examination center from September 2013 to June 2014 were preliminary screened, and those patients with symptoms of dry eye were investigated with questionnaires and diagnosed by basic lacrimal secretion test ( Schirmer l ) , breakup time of tear film ( BUT ) test and fluorescein ( FL) staining test .
RESULTS: There were 606 complaints of dry eye symptoms of the 1 000 premiers, distributed in random (60. 6%), and 432 employees were diagnosed (43. 2%). The positive rate of dry eye was 71. 3%. The causes of xerophthalmia, besides of gender, age, also include a variety of factors such as air-conditioner, heating, video terminals, sleep time, etc.
CONCLUSlON: Dry eye, an epidemic disease, has a variety of symptoms and complex etiology, the main causes of aerospace science and technology staff with dry eye is working environment and some local factors. We can give appropriate health guidance according to the different factors. Meanwhile the relevant series of dry eye screening should also be taken as a routine examination in ophthalmic examination.
5.Determination of residual organic solvents and macroporous resin residues in Akebia saponin D.
Qiao-han WANG ; Xiao-lin YANG ; Wei XIAO ; Zhen-zhong WANG ; Gang DING ; Wen-zhe HUANG ; Zhong-lin YANG
China Journal of Chinese Materia Medica 2015;40(10):1960-1964
According to ICH, Chinese Pharmacopoeia and supplementary requirements on the separation and purification of herbal extract with macroporous adsorption resin by SFDA, hexane, acetidine, ethanol, benzene, methyl-benzene, o-xylene, m-xylene, p-xylene, styrene, diethyl-benzene and divinyl-benzene of residual organic solvents and macroporous resin residues in Akebia saponin D were determined by headspace capillary GC. Eleven residues in Akebia saponin D were completely separated on DB-wax column, with FID detector, high purity nitrogen as the carry gases. The calibration curves were in good linearity (0.999 2-0.999 7). The reproducibility was good (RSD < 10%). The average recoveries were 80.0% -110%. The detection limit of each component was far lower than the limit concentration. The method is simple, reproducible, and can be used to determine the residual organic solvents and macroporous resin residues in Akebia saponin D.
Chromatography, Gas
;
instrumentation
;
methods
;
Drug Contamination
;
prevention & control
;
Organic Chemicals
;
analysis
;
Reproducibility of Results
;
Resins, Synthetic
;
chemistry
;
Saponins
;
analysis
;
isolation & purification
6.Determination of 27 elements in Maca nationality's medicine by microwave digestion ICP-MS.
Gui-fang YU ; Hai-jie ZHONG ; Jun-hua HU ; Jing WANG ; Wen-zhe HUANG ; Zhen-zhong WANG ; Wei XIAO
China Journal of Chinese Materia Medica 2015;40(23):4545-4551
An analysis method has been established to test 27 elements (Li, Be, B, Mg, Al, Sc, Ti, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, Ga, As, Sr, Mo, Cd, Sn, Sb, Ba, La, Hg, Pb, Bi) in Maca nationality's medicine with microwave digestion-ICP-MS. Sample solutions were analyzed by ICP-MS after microwave digestion, and the contents of elements were calculated according to their calibration curves, and internal standard method was adopted to reduce matrix effect and other interference effects. The experimental results showed that the linear relations of all the elements were very good; the correlation coefficient (r) was 0.9994-1.0000 (Hg was 0.9982) ; the limits of detection were 0.003-2.662 microg x L(-1); the relative standard deviations for all elements of reproducibility were lower than 5% (except the individual elements); the recovery rate were 78.5%-123.7% with RSD lower than 5% ( except the individual elements). The analytical results of standard material showed acceptable agreement with the certified values. This method was applicable to determinate the contents of multi-elements in Maca which had a high sensitivity, good specificity and good repeatability, and provide basis for the quality control of Maca.
Lepidium
;
chemistry
;
Mass Spectrometry
;
methods
;
Microwaves
;
Reproducibility of Results
;
Trace Elements
;
chemistry
;
isolation & purification
7.Chemical constituents from endophyte Chaetomium globosum in Imperata cylindrical.
Li SHEN ; Li ZHU ; Zhong-qi WEI ; Xiao-wen LI ; Ming LI ; Yong-chun SONG
China Journal of Chinese Materia Medica 2015;40(23):4645-4649
Isolation and purification of chemical constituents from solid culture of endophyte Chaetomium globosum in Imperata cylindrical was performed through silica gel column chromatography, gel filtration over Sephadex LH-20 and preparative HPLC. Nine compounds were obtained and their structures were determined as chaetoglobosin F(1), chaetoglobosin Fex(2), chaetoglobosin E(3) cytoglobosin A(4), penochalasin C(S), isochaetoglobosin D (6), N-benzoylphenylalaninyl-N-benzoyphenylalaninate(7), uracil(8) and 5-methyluracil(9), respectively, based on HR-MS and NMR data and comparison with literatures. Compound 7 was isolated from Chaeeomium sp. for the first time. In vitro cytotoxicity of compounds was evaluated using MTT mothed and 1,3,4 and 5 showed inhibition activity to the human cervical carcinoma cell HeLa with IC50 values of 99.43, 23.77, 97.92, 86.25 micromol x L(-1), while positive cotolocisnin Ad apno1ch alse IC50 24.33 micromol x L(-1).
Biological Factors
;
chemistry
;
pharmacology
;
Cell Line
;
Cell Survival
;
drug effects
;
Chaetomium
;
chemistry
;
Endophytes
;
chemistry
;
Humans
;
Molecular Structure
;
Poaceae
;
microbiology
;
Spectrometry, Mass, Electrospray Ionization
8.Optimization of extraction technology for salidroside, tyrosol, crenulatin and gallic acid in Rhodiolae Crenulatae Radix et Rhizoma with orthogonal test.
Xin LUO ; Xue-jing WANG ; Yi-wu ZHAO ; Wen-zhe HUANG ; Zhen-zhong WANG ; Wei XIAO
China Journal of Chinese Materia Medica 2015;40(18):3590-3593
The extracting technology of salidroside, tyrosol, crenulatin and gallic acid from Rhodiolae Crenulatae Radix et Rhizoma was optimized. With extraction rate of salidroside, tyrosol, crenulatin and gallic acid as indexes, orthogonal test was used to evaluate effect of 4 factors on extracting technology, including concentration of solvent, the dosage of solvent, duration of extraction, and frequency of extraction. The results showed that, the best extracting technology was to extract in 70% alcohol with 8 times the weight of herbal medicine for 2 times, with 3 hours once. High extraction rate of salidroside, tyrosol, crenulatin and gallic acid were obtained with the present technology. The extracting technology was stable and feasible with high extraction rate of four compounds from Rhodiolae Crenulatae Radix et Rhizoma, it was suitable for industrial production.
Chemical Fractionation
;
methods
;
Chemistry, Pharmaceutical
;
methods
;
Coumarins
;
isolation & purification
;
Drugs, Chinese Herbal
;
isolation & purification
;
Gallic Acid
;
isolation & purification
;
Glucosides
;
isolation & purification
;
Phenols
;
isolation & purification
;
Phenylethyl Alcohol
;
analogs & derivatives
;
isolation & purification
;
Rhizome
;
chemistry
;
Rhodiola
;
chemistry
9.Effect of bicuculline on first spike latency of the neurons in the inferior colliculus of mice.
Journal of Southern Medical University 2009;29(5):918-921
OBJECTIVETo evaluate the effect of bicuculline on the first spike latency of the neurons in the inferior colliculus of mice and investigate the role of GABA inhibition in sound signal processing of the neurons.
METHODSIn vivo extracellular recording was performed on the inferior colliculus of 13 BALB/c mice (4 to 5 weeks old) to record the neuronal response to pure tones. Bicuculline, a GABA-A receptor antagonist, was applied to the neurons iontophoretically through one channel in the three-barrel glass-pipettes. The first spike latency and other response properties of the characteristic frequency were recorded for analysis.
RESULTSA total of 30 well-isolated single neurons were recorded. Increased spike counts characterized 96% of the neurons, with either increased (40%) or decreased (60%) latency of neuronal responses. Characteristic frequency alterations occurred in 50% of the neurons with increased spike latency, and the minimum threshold showed linear changes.
CONCLUSIONGABAergic inhibition may participate in the latency formation and increased frequency selectivity of mouse inferior colliculus neurons by lateral inhibition. The changes in the first spike latency can be indicative of the information integration in GABAergic neurons at the synaptic level.
Acoustic Stimulation ; psychology ; Action Potentials ; drug effects ; Animals ; Bicuculline ; pharmacology ; Female ; GABA Antagonists ; pharmacology ; Inferior Colliculi ; physiology ; Male ; Mice ; Mice, Inbred BALB C ; Neurons ; physiology ; gamma-Aminobutyric Acid ; physiology
10.Meningeal hemangiopericytoma: a clinicopathologic study of 17 cases.
Hai-Bo WU ; Hai-Yan WENG ; Min DING ; Ping GU ; Xiao-Qiu WANG ; Wen ZHONG
Chinese Journal of Pathology 2011;40(4):251-252
Adult
;
Aged
;
Antigens, CD34
;
metabolism
;
Chondrosarcoma, Mesenchymal
;
metabolism
;
pathology
;
Diagnosis, Differential
;
Female
;
Follow-Up Studies
;
Hemangiopericytoma
;
metabolism
;
pathology
;
radiotherapy
;
surgery
;
Humans
;
Male
;
Meningeal Neoplasms
;
metabolism
;
pathology
;
radiotherapy
;
surgery
;
Meningioma
;
metabolism
;
pathology
;
Middle Aged
;
Neoplasm Recurrence, Local
;
Proto-Oncogene Proteins c-bcl-2
;
metabolism
;
Solitary Fibrous Tumors
;
metabolism
;
pathology
;
Vimentin
;
metabolism
;
Young Adult