The service of Traditional Chinese medical institutions consists Outpatient Care and Inpatient Care. The author summarizes the service situation of Traditional Chinese medical institutions in the last six years, by contrasting and analyzing the following quotas: the total number of treatment in the emergency door, the number of discharged patients, hospital admissions per 100 outpatient emergency treatment, rate of utilization of hospital beds, the average days of staying in hospital and doctors work efficiency, and then give some reasonable suggestions.

Ameloblastoma ; Humans ; Laryngeal Neoplasms ; Male ; Middle Aged

Objective To discuss pedicle screw system in the treatment of unstable thoracolumbar fraetures of the results of surgical treatment.Methods Pedicle screw system for thoracolumbar unstable fracture of 46 cases.Results 39 cases were followed up for an average of 17 months after the intervertebral space height and thoracolumbar physiological curvature satisfactory recovery.The average Cobb angle and the average spinal stenosis index has been markedly improved,Frankel raise a grade:three.Conclusion Posterior short section of the pediele screw system is the treatment of thoracic and lumbar fractures of the effective ways is satisfied with the reduction,reliable fixation.

Child ; Humans ; Male ; Paralyses, Familial Periodic ; diagnosis ; therapy

Objective To explore the therapeutic effectiveness and pathways of human umbilical cord mesenchymal stem cells (hUCMSCs) transplantation for acute hepatic failure in rats.Method hUCMSCs were isolated from umbilical cord with attachment culture method,and the surface antigens were tested by flow cytometry.Forty-eight male Sprague Dawley rats were randomly divided into four groups.The animal model of acute liver failure was induced by injecting intraperitoneally with 50％ olive oil solution of carbon tetrachloride (2.5 ml/kg).The treatment groups were injected with hUCMSCs suspension separately through the tail vein or injected into the liver 24 h post-modeling.Blood serum and liver tissues were collected at several time points to analyze the improvement of liver function and histological repair.Real-time PCR was used to detect the expression of human CK8,CK18 and AFP mRNA in liver tissues.Immunohistochemistry was used to detect the expression of human CK18 in liver tissues.Result There were statistically significant differences among liver functions after transplantation (P＜0.05).hUCMSCs improved histological status through enhancing hepatocellular regeneration and reducing inflammatory cells.Real-time PCR results showed that the expression of CK8,CK18 and AFP mRNA was obviously increased in the tail vein transplantation group and hepatic lobe injection transplantation group as compared with the model group (P＜0.05).Immunochemistry results revealed that transplanted hUCMSCs in animal liver could differentiate into functional hepatocyte-like cells that expressed human CK18 as hepatocyte-specific marker in the tail vein transplantation group and hepatic lobe injection transplantation group.No significant differences in histological repair and grade of differentiation were examined between the tail vein transplantation group and hepatic lobe injection transplantation group (P＞0.05).Conclusion hUCMSCs can prompt the repair of acute liver failure and enhance pathological repair.Transplanted cells in animal liver can differentiate into functional hepatocyte-like cells that expressing hepatocyte-specific markers.Transplantation of hUCMSCs via the tail vein or direct injection into the liver had the similar therapeutic effects.

Objective To investigate the effect of di-2-ethylhexyl phthalate (DEHP) on the proliferation of SK-N-SH human neuroblastoma cells and its underlying mechanism. Methods Cells were cultured in estrogen-free improved Dulbecco's Modified Eagle's Medium and then divided into 5 groups: no treatment (control group); treated with 17β-estradiol (E_2 group); treated with DEHP (DEHP group); treated with both E_2 and phosphatidylinositol-3-kinases (PI3K) inhibitor LY294002 (E_2 + LY294002 group); treated with both DEHP and LY294002 (DEHP + LY294002 group). The absorbance value (AV) was measured on day 0, 2, and 5. DNA proliferation index (PI) and apoptotic index (AI) were determined by flow cytometry on day 5. Caspase-3 protein, protein-serine-threonine kinase (Akt) and phosphor-Akt (Ser473) protein expression were analyzed by Western blot on day 5. Results The AV of All groups increased on day 2, and 5. The AV of E_2 and DEHP groups were higher than that of the control group (P<0. 001), but the AV of E_2 + LY294002 and DEHP + LY294002 groups were lower than those of E_2 and DEHP groups (P<0.01) on day 2 and 5. On day 5, PI of E_2 and DEHP groups were also higher than that of control (P<0.01). However, PI of E_2 + LY294002 and DEHP + LY294002 groups were lower than those of E_2 and DEHP group (P<0.01) on day 5. There was no significant difference in AI and caspase-3 protein expression among the groups. At the same time, phosphor-Akt (Ser473) protein expression of E_2 and DEHP groups increased obviously, compared with the control group. Compared with E_2 and DEHP groups, E_2 + LY294002 and DEHP + LY294002 groups decreased significantly. However, Akt protein expression was equal among those groups. Conclusions DEHP can promote the growth of SK-N-SH cells to a level similar to that of E_2, with activation of the PI3K/Akt signaling pathway.

A study on the reform of the evaluation method for college students' innovative experiment was carried out on account of the major problems existed in the present evaluation system.A set of evaluation indicators emphasizing process evaluation was constructed based on the theory of developmental evaluation.Also the performance appraisal methods for all indicators were determined.Practice suggested that the evaluation method based on developmental evaluation was able to promote the ability of scientific research and innovation of college students.

0.05),but these positive rates were notable decreased comparing with nPCR and immunohistochemistry techniques(P

Spinal cord injury (SCI) is a kind of severe central nervous system trauma causing motion and/or sensation dysfunction. MicroRNAs are short non-coding RNAs that suppress the translation of target genes, and play an important role in gene regulation involved in spinal cord development and SCI, which constitute novel targets for therapeutic intervention to promote repair and regeneration.

Objective To observe the antioxidative effect of Ginkgo biloba extract (EGb) on motor function after spinal cord injury (SCI) in rats. Methods 36 healthy adult Sprague-Dawley rats were divided randomly into EGb group (group A), methylprednisolone (MP) group (group B) and control group (group C) with 12 rats in each group. SCI model was made by Allen's mode (10 g×25 mm) at the level of the 9th thoracic vertebra (T9). Group A was given 40 mg/kg EGb daily by intraperitoneal injection, while group B was given 30 mg/kg MP at the first injection and then 5.4 mg/kg every 6 hours for 4 times. Group C received an equal volume of normal saline daily. On the 1st, 3rd and 7th days after SCI, 4 rats were selected randomly from each group. The motor function were assessed by Basso-Beattie-Bresnahan scale (BBB). The activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in serum were determined. Results At every time point after surgery, the BBB scores were significantly higher in groups A and B than in group C (P<0.05). Compared to group C,the activity of SOD increased and the content of MDA decreased significantly in groups A and B on the 3rd and 7th days after SCI (P<0.05).Conclusion EGb may reduce the level of oxidative stress and promote the recovery of the motor function in rats after SCI.