2.Effects of different fungi on symbiotic seed germination of two Dendrobium species.
China Journal of Chinese Materia Medica 2014;39(17):3238-3244
The epiphytic orchid, Dendrobium aphyllum and D. devonianum are used as traditional Chinese medicine, and became locally endangered in recent years because of over-collection. We test the effect of inoculations of endophytic fungi FDaI7 (Tulasnella sp.), FDd1 (Epulorhiza sp. ) and FCb4 (Epulorhiza sp.), which isolated from D. aphyllum, D. denonianum and Cymbidium mannii, respectively, on artificial substrate in these two Dendrobium species. In the symbiotic germination experiment, FDaI7 and FDd1 were effective for protocorm formation and seedling development of D. aphyllum and D. denonianum separately. After 60 days, 14.46% of the D. aphyllum seeds grown to protocorms and 12.07% developed to seedlings inoculated only with FDaI7, while contrasted with 0 when inoculated the other two isolates and non-inoculation treatment. However, in D. denonianum, seeds only grown to protocorms and developed to seedlings when inoculated with FDd1, the percentages were 44.36% and 42.91% distinguishingly. High specificity was shown in symbiotic germination on artificial substrate of Dendrobium. Protocorms could further develop to seedlings within or without light when inoculated the compatible fungi. However, light condition (12/12 h Light/Dark) produced the normal seedlings, while dark condition (0/24 h L/D) produced the abnormal seedlings. These may suggest that the development of young seedlings require light based on the effective symbiotic fungi. These findings will aid in seedling production of simulation-forestry ecology cultivation, conservation and reintroduction of Dendrobium.
Basidiomycota
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classification
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physiology
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Darkness
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Dendrobium
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classification
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growth & development
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microbiology
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Germination
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Host-Pathogen Interactions
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Light
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Plants, Medicinal
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classification
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growth & development
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microbiology
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Seedlings
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growth & development
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microbiology
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radiation effects
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Seeds
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growth & development
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microbiology
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Species Specificity
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Symbiosis
5.Analysis on the Use of Psychotherapeutic Agents in Our Hospital During 2004~2006
Qirong JIANG ; Yun YE ; Shunlin XIAO
China Pharmacy 2007;0(35):-
OBJECTIVE:To analyze the clinical application and trend of psychotherapeutic agents in our hospital.METH-ODS:The application of psychotherapeutic agents in our hospital from 2004 to 2006 was analyzed statistically in respect of drug varieties,consumption sum,DDDs and defined daily cost etc.RESULTS:The consumption sum of antipsychotic agents showed a year-on-year upward trend over the 3 year(379,100 yuan,438,800 yuan and 694,100 yuan,respectively).However,the consumption sum of antidepressants showed a downward trend over the 3 years(444,800yuan,440,200yuan,and 335,800 yuan,respectively).The total consumption of the two(antipsychotic agents and antidepressants) made up over 90% in the total psych-otherapeutic agents.CONCLUSION:Of the total consumption sum of psychotherapeutic agents,antipsychotic agents and antidepressants have made their way into the lead,of which,paroxetine,risperidone and olanzapine showing highest percentages.
6.Efficacy observation of Guan's quadruple therapy for kidney disease on the treatment of chronic renal failure.
Zheng ZUO ; Xiao-Mei CHEN ; Yun-Wu JIANG ; Xiao-Yun TANG ; Zun-Xin GUAN
Chinese Acupuncture & Moxibustion 2014;34(7):641-645
OBJECTIVETo compare the differences in the clinical efficacy between Guan's quadruple therapy for kidney disease and the conventional western medication in the treatment of chronic renal failure (CRF).
METHODSEighty patients were randomized into an observation group and a control group, 40 cases in each one. In the observation group, besides the physician instruction of low protein and low phosphorus diet in the two groups, Guan's quadruple therapy for kidney disease was applied, including (1) the isolated herbal moxibustion at Shenshu (BL 23) and Pishu (BL 21), or Ganshu (BL 18), Zusanli (ST 36) and Guanyuan (CV 4) alternatively; (2) acupuncture at the auricular points such as shen (CO10), jiaogan (AHR6), Shenshangxian (TG2), fei (CO14) and pizhixia (AT4); (3) injection of mixture of astragalus injection and lidocaine injection at Feishu (BL 13), Pishu (BL 21), Shenshu (BL 23) and the others; (4) modified Shenshuai Yihao decoction according to syndrome differentiation. In the control group, the conventional western medication was used. After 6 months of treatment, the differences were observed between the two groups in the clinical efficacy, serum creatinine (Scr), blood urea nitrogen (BUN), endogenous creatinine (Ccr) and 24 h urine protein quantitation (UPQ).
RESULTSIn the observation group, after treatment, the remarkably effective rate was 50.0% (20/40) and the total effective rate was 82.5% (33/40), which were superior to 25.0% (10/40) and 45.0% (18/40) in the control group (both P < 0.05). After treatment, the levels of Scr, BUN and 24 h UPQ were reduced apparently (all P < 0.05), and the level of Ccr was increased apparently in the two groups (both P < 0.05). After treatment, the levels of Scr, BUN and 24 h UPQ were reduced much more apparently in the observation group as compared with those in the control group (all P < 0.05).
CONCLUSIONGuan's quadruple therapy for kidney disease achieves a better efficacy on CRF compared with the conventional western medication. This therapy improves renal functions, relieves clinical symptoms and physical signs and benefits the life quality of patients.
Acupuncture Therapy ; Adult ; Combined Modality Therapy ; Drugs, Chinese Herbal ; administration & dosage ; Female ; Humans ; Kidney Failure, Chronic ; drug therapy ; physiopathology ; therapy ; Kidney Function Tests ; Male ; Middle Aged ; Young Adult
8.Epithelial neoplasms associated with osteoclast-like giant cells.
Yun-xiao MENG ; Ying JIANG ; Zhao-hui LU ; Jie CHEN
Chinese Journal of Pathology 2010;39(9):642-645
Adenocarcinoma, Mucinous
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pathology
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Breast Neoplasms
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pathology
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Carcinoma, Ductal, Breast
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pathology
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Carcinoma, Renal Cell
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pathology
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Carcinoma, Squamous Cell
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pathology
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Cystadenocarcinoma, Mucinous
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pathology
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Cystadenoma, Mucinous
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pathology
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Female
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Giant Cells
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pathology
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Humans
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Osteoclasts
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pathology
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Ovarian Neoplasms
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pathology
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Pancreatic Neoplasms
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pathology
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Thyroid Carcinoma, Anaplastic
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Thyroid Neoplasms
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pathology
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Tongue Neoplasms
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pathology
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Urologic Neoplasms
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pathology
9.Optimization of Fermentation Conditions of Lipase Produced by Pseudomonas cepacia PCL-3
Xiao-Feng WANG ; Jiang-Ke YANG ; Yun-Jun YAN ;
China Biotechnology 2006;0(10):-
The fermentation conditions of alkaline lipase producing by Pseudomonas cepacia PCL-3 were optimized.Based on the analysis of single factorial experiments,dextrin was the most suitable carbon source,peptone and urea were the suitable compound nitrogen sources among the examined materials.Three significant factors(urea,inoculum and initial pH) were selected from the eight factors related to lipase production by Plaekett-Burman method,and were further optimized with response surface analysis.And then,steepest ascent procedures were applied to define the optimal response region of the three factors.The obtained optimal conditions were urea 0.15%,inoculum 3.05% and initial pH 8.38,under which conditions,the enzyme activity was improved from 25.37 U/ml to 48.88 U/ml,enhanced 1.93 folds.Starting from the flask conditions,the highest lipase activity of 47.69U/ml was achieved by batch fermentation in a 10 L fermentor after 52 h of the cultivation.
10.Correlation between HCV genotype and anti-HCV antibody level in hepatitis C patients
Lingli JIANG ; Xueliang WANG ; Yun BAO ; Yanqun XIAO ; Jian ZHANG
Chinese Journal of Laboratory Medicine 2016;39(2):95-98
Objective To investigate the relationship between anti-HCV antibody level and hepatitis C virus genotype in the patients.Methods Total of 603 anti-HCV positive serum samples were collected during 2013 to 2014 by retrospective research method.HCV RNA were detected in anti-HCV positive samples by repeat test and the genotype were detected in HCV RNA positive samples.The distribution of anti-HCV level in different hepatitis C genotype patients was analyzed and the body's response to viral antibodies and viral genotype correlation with anti-HCV concentration interquartile range was explored.Rates among genotype groups were compared using chi-square test.Results Totally 412 of 603 (68.33%) samples were anti-HCV positive by double reagent screening.174(42.3%) samples were detected as HCV RNA positive.The distributions of different anti-HCV level in different genotype patients were 1a(n =8) 1/8,1/8,4/8,2/8;1b(n =112)25.9% (29/112),17.0% (19/112),25.9% (29/112),31.3% (35/112);2a(n =14)3/14,4/14,5/14,2/14;3a(n =11)3/11,6/11,2/11,0/11;3b(n =16)4/16,11/16,1/16,0/16;6a(n =8)2/8,2/8,1/8,3/8 with anti-HCV concentration interquartile range respectively.The anti-HCV concentration distribution was different in patients with different HCV genotypes.The anti-HCV concentration distribution in patients of 1 b,2a and 6a genotypes were evently,while anti-HCV level was relatively high in 1a (13.65) and relatively low in 3b (8.77).There were differences in different genotypes of antibody concentrations (x2 =35.2,P < 0.05).Conclusions There was correlation between anti-HCV level and HCV genotype.Because there were fewer cases in some genotypes,it was necessary to investigate more samples to corfirm the above results.